Study on Segmented Correlation in EEG Based on Principal Component Analysis

In order to explore the correlation between the adjacent segments of a long term EEG, an improved principal component analysis(PCA) method based on mutual information algorithm is proposed. A one-dimension EEG time series is divided equally into many segments, so that each segment can be regarded as an independent variables and multi-segmented EEG can be expressed as a data matrix. Then, we substitute mutual information matrix for covariance matrix in PCA and conduct the relevance analysis of segmented EEG. The experimental results show that the contribution rate of first principal component(FPC) of segmented EEG is more larger than others, which can effectively reflect the difference of epileptic EEG and normal EEG with the change of segment number. In addition, the evolution of FPC conduce to identify the time-segment locations of abnormal dynamic processes of brain activities,these conclusions are helpful for the clinical analysis of EEG.     

Speech Separation Based on Robust Independent Component Analysis

In this paper, we applied RobustICA to speech separation and made a comprehensive comparison to FastICA according to the separation results. Through a series of speech signal separation test, RobustICA reduced the separation time consumed by FastICA with higher stability, and speeches separated by RobustICA were proved to having lower separation errors. In the 14 groups of speech separation tests, separation time consumed by RobustICA was 3.185 s less than FastICA by nearly 68%. Separation errors of FastICA had a float between 0.004 and 0.02, while the errors of RobustlCA remained around 0.003. Furthermore, compared to FastICA, RobustlCA showed better separation robustness. Experimental results showed that RohustICA was successful to apply to the speech signal separation, and showed superiority to FastlCA in speech separation.     

Effect of Sleep Deprivation on Eye Movement Behavior in Flight Simulation

To examine the effect of sleep deprivation （SD） on eye movement be- havior in flight task, four subjects who were skilled in flight simulator participated in the experiment, which were asked to perform a level flight task in a flight simulator. Eye movement data and flight performance data were measured at the following hours： 11：00, 15：00, 04：00, 11：00, 15：00. The subjects workload and fatigue were assessed with the method of national aeronautics and space administration-task load index （NASA-TLX） and rating of perceived exertion （RPE）. Eye movement indices of average pupil area, av- erage saceade amplitude and average saccade velocity decreased during the 32 h of SD and they all showed significantly change in the final SD while the index of average fixa- tion time increased in the final SD. Flight performance deteriorated during the 32 h of SD, but not significantly. The feeling of fatigue and workload reported by subjects both increased during the 32 h of SD. Daily rhythm effects on the measured indices were also found, there were a obviously change at the hour of 04：00. 32 h of SD has obvious effect on eye movement behaviors which have close relation to fatigue because of SD. The eye movement measurement can be served as a tool to continually monitor fatigue online.     

States of Water in Hydrogels Containing with Glyceryl Methacrylate

Hydrogel materials were prepared by thermopolymerization with different content of glyceryl methacrylate and hydroxyethyl methacrylate. The different states of water in swelling hydrogels were described and studied by differential scanning calorimetry （DSC）. It was found that the hydrophilicity of GMA was stronger than HEMA, the water content and bound water of GMA hydrogel are higher than HEMA hydrogel. With the increase of GMA content, the content of free water in hydrogel increased. When GMA content was lower than 50%, the increase of GMA content also increased the content of bound water; but when GMA content was higher than 50%, the increase of GMA content decreased the content of bound water, which was caused by the chain hydrogen bond formed on the GMA chain with hydroxyl group each other.     

Attenuated Listeria monocytogenes as a cancer vaccine vector for the delivery of CD24, a biomarker for hepatic cancer stem cells

Attenuated Listeria monocytogenes （LM） is a promising candidate vector for the delivery of cancer vaccines. After phagocytosis by antigen-presenting cells, this bacterium stimulates the major histocompatibility complex （MHC）-I and MHC-II pathways and induces the proliferation of antigen-specific T lymphocytes. A new strategy involving genetic modification of the replication-deficient LM strain AdalAdat （Lmdd） to express and secrete human CD24 protein has been developed. CD24 is a hepatic cancer stem cell biomarker that is closely associated with apoptosis, metastasis and recurrence of hepatocellular carcinoma （HCC）. After intravenous administration in mice, Lmdd-CD24 was distributed primarily in the spleen and liver and did not cause severe organ injury. Lmdd-CD24 effectively increased the number of interferon （IFN）-7-producing CD8＋ T cells and IFN-7 secretion. Lmdd-CD24 also enhanced the number of IL-4- and IL-lO-producing T helper 2 cells. The efficacy of the Lmdd-CD24 vaccine was further investigated against Hepa1- 6-CD24 tumors, which were inguinally inoculated into mice. Lmdd-CD24 significantly reduced the tumor size in mice and increased their survival. Notably, a reduction of T regulatory cell （Treg） numbers and an enhancement of specific CD8＋ T-cell activity were observed in the tumor-infiltrating lymphocytes （TILs）. These results suggest a potential application of the Lmdd-CD24 vaccine against HCC.     

Soluble Expression in Escherichia Coli and Purification of Human Carboxylesterase 1

Objective： To achieve an optimized method for soluble expression of human carboxylesterase 1 （hCE-1） in escherichia coil and purification by Ni2＋-NTA agarose affinity chromatography, to get improved protein yield and purity for further development of hepatocellular carcinoma （HCC） diagnosis ELISA kits. Methods： The best antigen epitopes of hCE1 were predicted by comparing secondary structure, flexible regions, hydrophilicity, antigenic index surface probability of residues. Afterwards, pET-42a （＋） with a His-tag and a GST-tag was applied to form recombinant plasmid pET-42a （＋）/hCE1, which facilitated purification when using Ni2＋-NTA agarose affinity chromatography. Protein quality was measured by SDS-PAGE and BCA protein assay. Western-blot identification was also performed to ensure the correct expression of hCE1 protein. Results： The residues from 500 to 567 near C-terminal of hCE1 protein were considered the best epitopes which exhibited high hydrophilicity and high surface probability and relatively flexible secondary structure and low homology compared with hCE2 and hCE3. His-hCE1 500-567 fusion protein was achieved by IPTG-inducted expression with an expected mass of 42 kDa. After purification, the final product was specially identified, which reached over 95%purity and more than 10 mg/L of microbial culture. In Western blot, the purified fusion protein was recognized by anti-hCE1 monoclonal antibody, along with previous sequencing validation, which demonstrated the correct preparation of soluble hCE1 protein. Conclusion： This is an efficacious and affordable strategy to generate fusion hCE1 of high quality in E coli, which facilitates preparation of hCE1 monoclonal antibody and further HCC diagnosis research.     

The Study of Image Segmentation Based on the Combination of the Wavelet Multi-scale Edge Detection and the Entropy Iterative Threshold Selection

This paper proposes an image segmentation method based on the combination of the wavelet multi-scale edge detection and the entropy iterative threshold selection. Image for segmentation is divided into two parts by high- and low-frequency. In the high-frequency part the wavelet multiscale was used for the edge detection, and the low-frequency part conducted on segmentation using the entropy iterative threshold selection method. Through the consideration of the image edge and region, a CT image of the thorax was chosen to test the proposed method for the segmentation of the lungs. Experimental results show that the method is efficient to segment the interesting region of an image compared with conventional methods.     

Design of Calibration Device for Pulse Oximeter Simulator

Saturation of peripheral oxygen （SpO2） is one of the most important parameters of vital signs. Pulse oximeter based on near-infrared spectroscopy is commonly used as a non-invasive method to measure SpO2 yl. Currently, medical device manufacturers as well as metrology measurement agencies in China usually use pulse oximeter simulator as the commonly accepted functional calibration equipment for pulse oximeters. So far, no experimental protocol or devices can be used to test the accuracy and reliability of a pulse oximeter simulator. Therefore, a set of new metrology apparatus with the name of calibration device for pulse oximeter simulator have been designed in order to make a traceable system for the calibration or verification of pulse oximeter simulators. The principles and some research methods of this calibration device for pulse oximeter simulator will be discussed in this paper. Besides that, many experiments have been applied in order to guarantee the accuracy as well as traceability of this set of device.     

Numerical Simulation of Coronary Artery Bypass Graft with an Assistant Graft

The conventional bypass design is to implant a graft on the stenosed host artery allowing blood to flow bypass the stenotic artery. However, restenosis is a challenging problem which finally results in reoperation. The purpose of this paper is to propose a new bypass graft design of coronary artery with an assistant graft for the treatment of coronary artery stenosis. An additional assistant graft was employed in the new design compared with the conventional ETS anastomosis. Numerical simulations were performed by means of finite volume method using computational fluid dynamics （CFD） solver. Results demonstrated that the new anastomoses model provided a more smooth flow at the distal ETS anastomosis without any stagnation point on anastomotic bed and vortex formation in the heel region. Oscillatory shear index （OSI） and time-averaged wall shear stress gradient （TAWSSG） at the artery bed of the distal ETS anastomosis were reduced. The coronary artery bypass graft with an assistant graft is feasible to improve the local hemodynamics and diminish the probability of restenosis in the treatment of coronary artery stenosis.     

Coherent Point Drift Registration Combined with Image Feature and its Application

A key step of constructing active appearance model is requiring a set of appropriate training shapes with well-defined correspondences. In this paper, we introduce a novel point correspondence method （FB-CPD）, which can improve the accuracy of coherent point drift （CPD） by using the information of image feature. The objective function of the proposed method is defined by both of geometric spatial information and image feature information, and the origin Gaussian mixture model in CPD is modified according to the image feature of points. FB-CPD is tested on the 3D prostate and liver point sets through the simulation experiments. The registration error can be reduced efficiently by FB-CPD. Moreover, the active appearance model constructed by FB-CPD can obtain fine segmentation in 3D CT prostate image. Compared with the original CPD, the overlap ratio of voxels was improved from 88.7% to 90.2% by FB-CPD.     

Effect of Exposing Low-Frequency Electric Fields on the Proliferative Capacity and Morphological Features of Olfactory Ensheathing Cells in vitro

Olfactory ensheathing cells （OECs） transplanted into the damaged spinal cord may be considered as a valuable remedy explorations for spinal cord repair. The proliferation of transplanted olfactory ensheathing cells depends on various environmental factors and effective cues, which may include electrical fields （EFs）. In this study, we investigated the proliferative capacity, morphologic alterations of olfactory ensheathing cells derived from neonate rat that occurd when exposed to two EFs of 20 Hz, 50 mV and 20 Hz, 100 mV for 6 h. For both EF treatments, the MTT results revealed that the cellular proliferation of exposed group during the last 6 h of the experiment was statistically higher than that of control group. Then, we investigated morphological structure changes in the cells stained by Coomassie brilliant blue. Compared with control group, most of cells were present at intensively proliferating appearance including the microfilaments were long and thick and the accumulated appearance of cells. It is conceivable that electrical fields as a new approach may promote the growth and proliferation of OECs and may be engineered to control the survival of transplanted OECs in injured spinal cord. Although our results have been suggesting that EFs may be non-chemical strategies for cell proliferation, the fundamental mechanisms remain to be elucidated.     

国外心理危机干预研究

本文对近年来20余篇外文文献进行总结,概览了国外心理危机干预的最新研究现状,重点阐述了心理危机干预的概念、评估方法、基本模式和严重突发事件应急报告技术.提出了在危机干预中存在的不足,指出了加强危机干预研究的意义,为我国心理危机干预的进一步发展提供借鉴.     

A New Computer Assisted Orthopaedic Surgery System： WATO

Background： The traditional manual orthopaedic technology heavily re- lies on a surgeon＇s experience, so it certainly increases the instability of the surgery. Therefore, computer assisted orthopaedic surgery （CAOS） is becoming a hot research topic for its high accuracy and stability. We developed a new CAOS system WATO, which is mainly designed for total knee replacement （TKR）. Methods： WATO system provides the interactive software for a surgeon＇s preoperative planning. Based on its two infrared cameras, infrared markers and infrared probe, WATO system gives a simple surgical positioning procedure of femur and tibia without additional surgery for the placement of fiducial markers. According to the reference alignment axis from positioning procedure, a surgeon can move the robot of WATO system to do accurate bone resection. Safety checking is also considered in WATO system. Results： Extensive experiments were conducted on phantoms and cadaver bones to verify the accuracy and stability of WATO system. Experimental results showed that TKR using WATO system had better performance compared with traditional and navigated TKR. Conclusion： WATO system shows its superiority in TKR, and has a broad application prospect in the future. We will develop its new functions for other orthopaedic surgery such as total hip replacement （THR）. Current disadvantages such as bigger skin incision have to be resolved in the future.     

开设医疗器械类高职专业面临的问题及对策

改革开放以来我国经济高速增长,对各类人才的需求日益迫切,从20世纪末高校扩招以来高等教育飞速发展,因面向的主体不同致使我国的高等教育被人为的划分为普通高等教育和高等职业教育,相对普通高等教育来讲高等职业教育起步晚、受重视程度明显不足,但随着市场经济的日益成熟,市场这个无形的手对教育结构的调整显现了惊人的力量,特别是近年以来各级政府教育主管部门对高等职业院校的关注日益增强,如雨后春笋般拔地而起的高等职业院校有力地证明了这点。     

Analysis of Heart Rate Variability in Chinese PLA Navy Global Visiting Task Group

Objective： To analyze the heart rate variability in Chinese PLA navy global visiting task group. Methods：We analyzed the heart rate variability in Holter in 77 men and 4 women for 5-15 days before voyage, and 65-75 and 115-125 days after voyage, and 29 men and 3 women for 5-15 days after having finished voyage. Results：NN50 and VLF were lower in 77 men and 4 women for 65-75 days after voyage than that was 5-15 days before voyage （P＆lt;0.01）. SDANN was lower in 77 men and 4 women for 115-125 days after voyage than that was 65-75 days after voyage （P＆lt;0.01）. SDNN, SDANN, SDNN index, RMSSD, NN50, PNN50,Triangular index, VLF, HLF, VAI and VLI were lower in 77 men and 4 women for 65-75 days after voyage than that was 5-15 days before voyage （P＆lt;0.01）.Conclusion： These findings suggest that voyage may reduce heart rate variability for a long time.     

外训后勤部队军人心理应激特点及相关因素的路径分析

目的 探讨外训后勤部队军人心理应激特点及相关因素.方法 采用军人心理应激自评问卷（PSET）,症状自评量表（SCL-90）,抑郁自评量表（SDS）,状态-特质焦虑问卷（STAI）作为评价工具,对外训后勤部队的224名军人进行调查.结果 224名外训军人PSET量表总评T分为（49.88±9.89）,其中心理应激程度较高的有7人（T分≥70,3.1％）.在警觉性增高（24.6％）、睡眠不佳（10.3％）、兴趣减退（6.7％）、心身疲倦（6.7％）方面发生人数较多.外训军人不同性别、集体氛围、级别、文化程度PSET得分存在显著差异（t/F=2.44,2.91,5.01,4.01;P＜0.01,P＜0.05）.在路径分析中,SCL-90阳性项目数、特质焦虑、SDS对心理应激有直接效应;而恐怖和敌对具有间接效应.结论 外训后勤部队军人存在心理应激反应,心理应激受军人心理健康、特质焦虑、抑郁的直接影响,提高军人心理健康水平和心理素质有利于降低军人应激水平.     

Expression of IL-1α, IL-6, TGF-β, FasL and ZNF265 During Sertoli Cell Infection by Ureaplasma Urealyticum

To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum （UU）-induced model. We investigated the expressions of IL-1α, IL-6, TGF-β, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-β and FasL, the high levels of IL-1α and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to those of TGF-β and FasL in vitro and in vivo for Sertoli cells infected with UU.     

Biomechanical Effect of Chinese Immobilization Using Little Splint

Immobilization using little splint is an original innovation of Chinese people for the fracture fixation, which is simple to use and clinically effective. It was found that Chinese immobilization using little splint can make the non-invasive, uncovering, and trouble free healing of bone fracture via harmonious unity of the structure stability and the force balance, of the motion stability and the stress adaptability, of the constant and discontinuous physiological stress. The biomechanical effect of Chinese immobilization using little splint, including entirety, dynamic, and functional fixity, is the root cause of its inheritance and the use up to now, and also is a direction of today＇s fracture fixation towards personalization, individuality and entirety.     

A Miniaturized System for Neural Signal Acquiring and Processing

To collect neural activity data from awake, behaving freely animals, we develop miniaturized implantable recording system by the modem chip：Programmable System on Chip （PSoC） and through chronic electrodes in the cortex. With PSoC family member CY8C29466,the system completed operational and instrument amplifiers, filters, timers, AD convertors, and serial communication, etc. The signal processing was dealt with virtual instrument technology. All of these factors can significantly affect the price and development cycle of the project. The result showed that the system was able to record and analyze neural extrocellular discharge generated by neurons continuously for a week or more. This is very useful for the interdisciplinary research of neuroscience and information engineering technique. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.