稀土掺杂对纳米TiO2薄膜微结构及其抗凝血性能的影响

采用溶胶-凝胶法制备了均匀的TiO2薄膜及稀土掺杂TiO2薄膜。通过掺入不同稀土元素及浓度,利用X射线衍射、X光电子能谱、扫描电子显微镜等材料表征方法,研究了掺杂稀土元素对TiO2薄膜微结构、成分、形貌及亲水性的影响。结果表明:在TiO2薄膜中添加适量的稀土元素能细化TiO2晶粒,薄膜的亲水性明显提高。血小板粘附实验和动态凝血实验表明:掺杂稀土有利于改善TiO2薄膜的抗凝血性能,适当的掺杂量能使薄膜的血液相容性得到优化。分析了稀土元素对材料的血液相容性能的影响机制。     

聚多巴胺辅助溶胶-凝胶TiO_2薄膜表面固定牛血清白蛋白

在钛表面涂覆溶胶-凝胶TiO2薄膜,再利用聚多巴胺薄膜结合牛血清白蛋白(BSA)分子,以改善血液相容性。X射线光电子能谱分析表明TiO2薄膜表面形成了聚多巴胺薄膜和BSA分子层。接触角测试结果表明聚多巴胺薄膜和BSA分子层使试样的接触角升高,但表面能和界面张力下降。血液相容性实验表明,与TiO2涂层试样相比,结合BSA分子的试样具有更好的抗凝血性能和抗血小板聚集性能。     

氧化镧掺杂非晶碳薄膜的血液相容性

采用射频磁控溅射方法制备了氧化镧掺杂的非晶碳薄膜.利用拉曼光谱,光电子能谱等方法, 研究了氧化镧掺杂对非晶碳薄膜的微结构、成分、形貌及亲水性的影响.结果表明:在非晶碳薄膜中掺入氧化镧后,薄膜中sp3/sp2的比率发生显著变化,薄膜的疏水性明显提高.血小板粘附实验表明,掺杂氧化镧有利于提高非晶碳薄膜的抗凝血性能,适当的掺杂量能使薄膜的血液相容性得到优化.并分析了氧化镧对材料的血液相容性能的影响机制.     

血液相容性氧化钛薄膜最佳掺杂含量的理论计算

我们把一个从对透明导电薄膜材料研究中得到的最佳掺杂含量定量理论推广到血液相容性氧化钛薄膜材料，该理论建立了电子薄膜材料的某一物理性能与晶体结构，制备方法和掺杂剂含量之间的联系，给出了一个能够拟合实验曲线的具有确定物理意义的抛物线方程，该方程的极值点确定了最佳掺杂含量与晶体结构和制备方法之间的定量关系，进而得到了一个掺杂最佳含量的表达式，分析血液相容性氧化钛薄膜材料的钽掺杂改性的实验结果，应用最佳掺杂含量表达式定量计算了血液相容性氧化钛薄膜材料的钽最佳掺杂含量。     

离子束合成Ti—O薄膜对热解碳生物材料表面改性的研究

采用离子束增强沉积在低温各向同性热解碳表面进行了Ｔｉ－Ｏ薄膜合成研究，获得了化学计量比及非化学计量比的ＴｉＯ２薄膜，采用动态凝血时间测定、血小板粘附行为研究、全血动态接触研究、溶血率测定等方法进行了血液相容性评价，用声发射薄膜结合力测定、针盘磨损试验及显微硬度分析研究了热解碳表面薄膜的力学性质。研究表明，经Ｔｉ－Ｏ薄膜沉积，热解碳的血液相容性和表面力学性质获得了改善，提出了血液相容性机理模型。     

金属表面TiO2薄膜的溶胶-凝胶法制备及其血液相容性研究

通过溶胶 凝胶法制备TiO2 薄膜对 316L不锈钢和NiTi形状记忆合金进行表面改性处理。研究发现 ,经 5 0 0℃处理 1h的薄膜结构致密 ,膜层均匀平滑 ,薄膜主要由锐钛矿相TiO2 构成 ,随热处理温度的提高 ,锐钛矿相逐渐转变为金红石相。电化学腐蚀和动态凝血时间及溶血率测试表明 ,通过溶胶 凝胶法制备TiO2 膜进行表面改性的 316L不锈钢和NiTi合金的抗模拟体液腐蚀性提高 ,动态凝血时间延长 ,溶血率下降 ,说明溶胶 凝胶法制备TiO2 膜可以提高金属植入物的血液相容性     

TiO2-x薄膜与热解碳血液相容性的对比研究

本文用血小板粘附试验证实了TiO2 x薄膜具有优于热解碳的血液相容性 ;并通过对材料表面 (界面 )能参数与血浆蛋白吸附关系的分析 ,阐述了两种材料表面蛋白质的不同吸附行为是导致其血液相容性差异的重要原因。     

TiO2-x薄膜与热解碳血液相容性的对比研究

本文用血小板粘附试验证实了TiO2-x薄膜具有优于热解碳的血液相容性;并通过对材料表面(界面)能参数与血浆蛋白吸附关系的分析,阐述了两种材料表面蛋白质的不同吸附行为是导致其血液相容性差异的重要原因.     

金属医用材料表面微磁场改善的血液相容性

背景:在316L不锈钢、NiTi合金的表面用溶胶凝胶法制备含SrFe12O19磁性粉末的TiO2薄膜,磁化后在材料表面形成微磁场,可以延长其动态凝血时间,降低其溶血率,改善材料的血液相容性。目的:讨论材料表面微磁场与血液相互作用的机制。方法:用柠檬酸法制备SrFe12O19粉末,用X射线衍射仪(XRD)、扫描电镜(SEM)等分析了磁性粉末的成分及形貌,用磁强计检测了SrFe12O19粉末的磁性能;用溶胶凝胶法在316L不锈钢、NiTi合金的表面制备含SrFe12O19磁性粉末的TiO2薄膜,并用X射线衍射仪分析了薄膜成分;通过动态凝血时间和溶血率的测定结果分析材料表面微磁场与血液相容性的关系。结果与结论:材料表面产生的磁场阻碍了血液中Ca2+、和纤维蛋白原、血小板及红细胞等分子在材料表面上的黏附,从而抑制了凝血反应的进行,因而具有抗血栓的作用,提高了材料与血液之间的相容性。     

金属生物材料表面微磁场对血液相容性影响的研究

用溶胶-凝胶法在316L不锈钢及NiTi合金基片上制备含SrFe12O19磁性粉末的TiO2薄膜，磁化后在材料表面建立微磁场。用透射电镜（TEM）分析磁性粉末的尺寸及形貌，并用X射线（XRD）、粗糙度仪对薄膜的成份及表面粗糙度进行分析。腐蚀实验表明，含磁粉的TiO2薄膜能够减少金属材料受到体液的腐蚀，阻止毒性镍离子等的析出。动态凝血时间和溶血率的测定结果表明，用含磁粉的TiO2薄膜产生的微磁场可使316L不锈钢和NiTi合金动态凝血时间延长，溶血率下降，说明试样表面的微磁场使材料具有较好的血液相溶性。     

Antithrombogenic investigation of surface energy and optical bandgap and hemocompatibility mechanism of Ti(Ta(+5))O2 thin films

Recent improvements in the antithrombogenic properties of blood contacting biomaterials permit a hybrid design of layers for biomedical applications such as artificial heart valves and stents. Using magnetron sputtering and thermal oxidation, titanium oxide thin films containing tantalum. Ti(Ta(+5))O2, are fabricated to meet the challenge of enhanced hemocompatibility. The blood compatibility is evaluated in vitro by clotting time and platelet adhesion measurement, and in vivo experiments are also conducted. The Ti(Ta(+5))O2 films exhibit attractive blood compatibility exceeding that of low isotropic pyrolytic carbon. Physical properties such as surface energy and semiconductivity are found to play important roles. Our calculated results reveal that the smaller surface force gamma(s) of the film and the smaller blood film interfacial tension gamma(c,blood) are partially responsible for the enhancement of the blood compatibility. Based on the optical bandgap model, the film possesses better hemocompatibility because its optical bandgap of 3.2 eV is wider than that of fibrinogen having a bandgap of 1.8 eV. These factors result in thinner protein layers on the film surface, less protein denaturing, and overall excellent antithrombogenic properties.     

热解碳人工心脏瓣膜材料表面改性研究

对氮离子注入热解碳及在热解碳表面离子束增强沉积钛氧化物薄膜进行了研究,采用Ｘ光电子能谱仪,俄歇电子能谱仪测定了表面改性层的成价和价态,用四探针方法测定了表面电阻率,用接触角方法测定了材料表面能,用动态凝血时间及血小板粘附评价了表面改性层的血液相容性。研究表明,热解碳经表面Ｎ离子注入后有碳氧化合物形成,其血液相容性有所改善,而采用离子束增强沉积合成ＴｉＯ２－ｘ薄膜则使热解碳备注相 容性获得了显著改善     

Wear resistance of experimental titanium alloys for dental applications

The present study evaluated microstructure, microhardness and wear resistance of experimental titanium alloys containing zirconium and tantalum. Alloys were melted in arc melting furnace according to the following compositions: Ti-5Zr, Ti-5Ta and Ti-5Ta-5Zr (%wt). Hemispheres and disks were obtained from wax patterns that were invested and cast by plasma. Microstructures were evaluated using optical microscopy and X-ray diffraction (XRD) analysis and also Vickers microhardness was measured. Hemispherical samples and disks were used for 2-body wear tests, performed by repeated grinding of the samples. Wear resistance was assessed as height loss after 40,000 cycles. The data were compared using ANOVA and post-hoc Tukey test. Ti-5Zr presented a Widmanst?tten structure and the identified phases were α and α' while Ti-5Ta and Ti-5Ta-5Zr presented α, β, α' and α" phases, but the former presented a lamellar structure, and the other, acicular. The microhardness of Ti-5Zr was significantly greater than other materials and cp Ti presented wear resistance significantly lower than experimental alloys. It was concluded that wear resistance was improved when adding Ta and Zr to titanium and Zr increased microhardness of Ti-5Zr alloy.     

氧化钛薄膜的血液相容性机理探讨

考究了影响氧化钛薄膜血液相容性的因素,提出了血液相容性机理的看法,认为血液相容性是表面能和功函数共同作用的结果。表面能决定蛋白质吸附,而功能函决定蛋白质的分解,并给出了一个表面能区域,指出一个良好血液相容性材料,不仅要有合 表面能,还要有较小的功函数。     

Preparation and characterization of tantalum functional coating on titanium implant北大核心

BACKGROUND: Titanium (Ti) alloy is widely used in clinical orthopedics, because of its good biocompatibility. However, as a biological inert material, it is lack of osteoinductive activity, which is easy to cause implant loosening. Therefore, it is necessary to modify the surface of titanium implant for enhancing its osteogenic activity. OBJECTIVE: To prepare Tantalum (Ta) functional coating on titanium surface by sol-gel method, and characterize the physicochemical properties and osteogenic properties of the coating. METHODS: Ta functional coating was prepared on the surface of medical Ti sheet by sol-gel method (named as Ti-Ta). The surface morphology and element composition of the coatings were characterized by scanning electron microscope and energy spectrum analysis. The surface hydrophilicity of Ti sheet, Ta sheet, and Ti-Ta coatings was evaluated by contact angle test. Rabbit bone marrow mesenchymal stem cells were co-cultured with the Ti, Ta and Ti-Ta samples. Cell adhesion morphology on the material surface was observed by scanning electron microscope. Cell adhesion and survival were observed by fluorescence staining. Cell proliferation activity was detected by CCK-8 method. Alkaline phosphatase staining and alizarin red S staining were used to evaluate the osteogenic differentiation ability of cells on the material surface. RESULTS AND CONCLUSION: (1) Scanning electron microscope images showed that nanoparticles were uniformly distributed on the surface of Ti-Ta sample and no cracks appeared. The elemental analysis showed that elements on the coating were mainly composed of Ta, O, Ti. Ti-Ta surface had better hydrophilicity than that of Ti and Ta. (2) Twelve hours after seeding, cells spread and adhered to the surface of Ti-Ta, protruding a large number of filamentous pseudopodia to the distance and connecting with adjacent cells. For cells on the surface of Ti and Ta, they showed long fusiform shapes, with a small amount of filamentous pseudopodia extending around. (3) The fluorescence staining results of living/dead cells showed that at 72 hours after seeding, a large number of living cells were observed on all surfaces. The number of adherent cells was more than that of Ti sheet and Ta sheet, and the number of living cells on the surface of Ti- Ta coating was more than that on Ti sheet and Ta sheet (P < 0.05). (4) CCK-8 results showed that Ti-Ta had the strongest ability to promote cell proliferation (P < 0.05). (5) The content of alkaline phosphatase and the number of calcium nodules on the surface of Ti-Ta surfaces were much higher than those on the Ti and Ta surfaces. (6) In conclusion, the Ti surface modified by Ta coating was more favorable for the adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells. © 2022, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.     

纯钛种植体表面微弧氧化涂层的生物相容性

背景：各种纯钛种植体表面微弧氧化涂层效果不尽相同。 目的：观察3种不同微弧氧化涂层种植体钛片对小鼠成骨细胞的细胞增殖、碱性磷酸酶活性和β1-integrin的基因表达水平的影响。 方法：采用国际常用小鼠成骨细胞系(MC3T3-E1),3种不同涂层钛片作为影响因素,纯钛作为对照,采用MTT法和电镜法观察细胞附着和细胞增殖,PNPP法测定碱性磷酸酶的活性,RT-PCR法检测β1-integrin在小鼠成骨细胞中的表达。 结果与结论：MTT值、碱性磷酸酶值、β1-integrin的基因表达水平和电镜观察均显示含钙、磷、镁、锌元素的二氧化钛涂层钛片生物相容性最好,含钙磷盐的二氧化钛涂层钛片次之,二氧化钛涂层钛片最差。小鼠成骨细胞在其多孔,含有钙、磷、镁、锌元素表面的黏附及增殖最优。     

Effects of fluoride concentration and elastic tensile strain on the corrosion resistance of commercially pure titanium.

The corrosion resistance of commercially pure (CP) titanium in 1% NaCl + 0 approximately 1% NaF solution (pH = 6) under different elastic tensile strains was investigated by using electrochemical impedance spectroscopy (EIS) measurement technique. The polarization resistance (Rp), which is inversely proportional to the corrosion rate, of CP titanium in the test solution was obtained from the EIS data. Different elastic tensile strains, namely 0%, 1%, 2%, and 4%, were applied on the test specimens by using a tensile test machine during the corrosion tests. Corrosion morphology was characterized by using a scanning electron microscope. Surface chemical analyses were performed by using energy dispersive spectroscopy and X-ray photoelectron spectroscopy. The results showed that the NaF concentration and the elastic tensile strain had a statistically significant influence on the Rp (P<0.001). The Rp decreased on increasing the NaF concentration and the elastic tensile strain. When the NaF concentration was lower than 0.10%, the Rp value (> 3.4 x 10(5) omega cm2) was mainly ascribed to the formation of a protective titanium dioxide (TiO2) on the metal surface, regardless of the elastic tensile strain applied. However, when the NaF concentration was higher than 0.1%, the protectiveness of TiO2 was destroyed by fluoride ions, leading to severe corrosion of CP titanium.     

Photocatalytic self-cleaning keratins: A feasibility study

Anatase nanocrystals were successfully synthesized and deposited on protein keratin-type wool fibers with good compatibility and significant photocatalytic self-cleaning activity using the sol-gel process. Due to the low chemical resistance and liability to photo-degradation of protein materials, the effect of the acid catalyst used in the sol synthesis was studied. The sols were prepared using oxidizing and non-oxidizing catalysts, nitric acid and hydrochloric acid, respectively, for the hydrolysis and condensation reactions of the titanium dioxide precursor. The size distribution and crystallinity of the sols were characterized by X-ray diffraction spectroscopy and photon correlation spectroscopy. The compatibility of sol formulations and wool is thoroughly compared and discussed by analyzing fibers photo-degradation, surface morphology and self-cleaning properties including stain degradation and colorant decomposition. The UV absorption and mechanical properties of wool fibers before and after coating are also discussed.