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1.
连云港市723例献血员弓形虫感染调查   总被引:1,自引:1,他引:0  
弓形虫病(Toxoplasmosis)又称弓形体病。为人兽共患性疾病。据文献报道,弓形虫感染在我国也普遍存在,国内报道也屡见不鲜。其传播途径除通过饮食、水源污染、接触感染牲畜外,也可以通过输血感染。国内有关献血员弓形虫感染的情况报道甚少。为此,我们对723例献血员进行了弓形虫循环抗原、抗体IgG、IgA、IgM的检测,现将结果报告如下。  相似文献   

2.
638名供血者弓形虫感染状况调查   总被引:2,自引:0,他引:2  
弓形虫病是由病原体弓形虫感染引起的一种人兽共患性疾病,在我国各地分布很广,人群平均感染率在4%-9%[1].其传播途径除饮食、接触感染的禽畜、母婴垂直传播外,亦可通过输血感染.为了解本地供血者弓形虫感染状况,本文对638名供血员的血清进行弓形虫抗原、抗体的检测.现将其结果报道如下.  相似文献   

3.
弓形虫病(Toxoplasmosis)又称弓形体病。为人兽共患性疾病。据文献报道,弓形虫感染在我国各地普遍存在,报道屡见不鲜。其传染途经除通过饮食、水源污染和密切接触感染牧畜外,本人抵抗力的强弱也有关。国内尚未见有从事放射性工作人员弓形虫感染情况的调查。为此,我们进行了调查,现将结果报告如下。  相似文献   

4.
弓形虫病是一种世界广泛流行的人兽共患寄生虫病,在人类多呈隐性感染或亚临床经过,而免疫功能低下时,容易发生原发弓形虫感染,尤其是孕期感染,可影响胎儿发育,引起流产和先天性弓形虫病,是人类围产期隐性感染影响新生儿出生缺陷的主要病原体之一。近年来国内已在开展围孕期弓形虫抗体筛选检测项目,然而,对检测结果与受检者感染状态的评估很少有系统性方案报道。本研究对孕早期系列血清检测弓形虫抗体IgG与IgM,拟对孕早期弓形虫感染进行综合评价。  相似文献   

5.
ELISA弓形虫IgG抗体试剂盒的研制   总被引:2,自引:0,他引:2  
弓形虫病 (Toxoplasmosis)是一种人兽共患的寄生虫病。病原体为弓形虫 (Toxoplasmagondii) ,属球虫类原虫 ,广泛分布于世界各地 ,人和动物普遍易感。许多欧美国家的血清试验阳性率达 2 5~ 50 %。我国自 1 980年以来 ,在全国范围内进行了人和动物弓形虫病的流行病学调查 ,所调查地区均发现弓形虫感染 ,说明我国弓形虫感染和弓形虫病分布十分广泛 ,人体感染弓形虫后 ,体内最先产生的是循环抗原 (CAg) ,最早出现的抗体是IgM抗体 (病期的 7~ 8天 ) ,它是早期弓形虫感染诊断的主要指标 ,随后出现IgA抗体…  相似文献   

6.
弓形虫病(toxoplasmosis)又称弓形体病,是由弓形虫所引起的一种人兽共患性疾病,广泛流行于世界各地。当人体遭受各种疾病而免疫功能降低时极易感染此病,且大多为隐性感染,临床表现也复杂多样,肿瘤病人伴发弓形虫感染,甚至造成死亡的文献已有报道。弓形虫病在我国亦普遍存在。本文总结374例肺部良、恶性疾病患者血清弓形虫抗原、抗体的检测结果,现报告如下。  相似文献   

7.
哈尔滨地区孕早期妇女弓形虫感染的检测分析   总被引:5,自引:2,他引:3  
弓形虫病是一种人畜共患疾病 ,广泛流行于世界各地。弓形虫病对人类健康的最大危害是造成先天性弓形虫感染 ,孕早期感染弓形虫时 ,可引起胎儿宫内感染而导致流产、胎儿中枢神经系统损害等 ,检测弓形虫特异性IgM抗体 ,可确定患者为近期感染。为探讨孕早期妇女的弓形虫感染情况 ,我们对 15 68例孕 5 12周妇女进行了血清弓形虫IgM抗体检测 ,现将结果报告如下。资料与方法一、检测对象 :1996年 7月至 1999年 6月 ,对来我院进行孕期检查的 15 68例妇女进行血清弓形虫IgM抗体检测 ,年龄 2 1 33岁 ,孕 5 12周。二、检测方法 :采用ELI…  相似文献   

8.
肿瘤与弓形虫感染的关系探讨   总被引:1,自引:0,他引:1  
弓形虫病(Toxoplasmosis)是一种人兽共患性疾病,临床表现复杂多样,人体多为隐性感染,当机体受到其它疾病侵害或免疫力降低时,可转为急性发作。肿瘤病人伴发弓形虫感染,甚至造成死亡,文献已有报道。为了探讨恶性肿瘤与弓形虫感染的关系,我们特搜集国内外有关文献,对肿瘤患者弓形虫的易感性、以及肿瘤与弓形虫并存的相互关系等问题作一综述,以供参考。 肿瘤患者弓形虫的易感情况 天津医学院赵红等用ELISA法对肿瘤病人进行了血清弓形虫抗体的检测,以了解天津地区肿瘤患者弓形虫的感染情况。肿瘤患者240例,阳性40  相似文献   

9.
输血感染弓形虫及其对策   总被引:1,自引:0,他引:1  
弓形虫病(toxoplasmosis)又称弓形体病,是由弓形虫(toxoplasma gondii)所引起的一种人畜共患性疾病,广泛流行于世界各地。弓形虫病在我国分布很广,全国各省、市、自治区(包括台湾省)均有弓形虫感染的报道,人群感染率为4.86%。弓形虫是一种细胞内的寄生原虫,常存在于脑、肌肉和其它器官中。在免疫功能正常的人体内,繁殖力受到抑制,常形成包囊,而无临床症状,但在新生儿或免疫功能不良的病人中,则大量繁殖,并出现各种症状。由于弓形虫寄生部位及机体反应性的不同,发病者临床表现复杂,主要侵犯眼、脑、心、肝、肺及淋巴结等。孕妇受染后弓形虫可通过胎盘感染胎儿,  相似文献   

10.
弓形虫抗体在人体内的消长情况及其临床意义   总被引:3,自引:2,他引:1  
弓形虫病(Toxoplasmosis)是由弓形虫引起的一种人兽共患的寄生虫病,分布范围广,对人类危害大,孕妇感染弓形虫后,可通过胎盘垂直传播给胎儿,损害胚胎发育,引起胎儿严重的神经系统发育畸形和智力障碍,降低人口素质,甚至引起死亡。自五十年代起,国外学者从不良妊娠结局者的月经血、乳汁、恶露、胎盘、羊水及胚胎等标本中分离出弓形虫。近期国内研究表明,我国孕妇弓形虫感染亦较普遍,对下一代的健康带来严重威胁。因此,弓形虫病已成为围产医学中一个重要的原虫病。 弓形虫病最可靠的诊断方法是确认病原,但一般比较困难,因此,应用血清学方法检测弓形虫抗休已被公认是重要的辅助诊断手段。 弓形虫病的血清学方法种类很多,如Sabin-Fe-  相似文献   

11.
Toxoplasmosis in heart and heart and lung transplant recipients.   总被引:9,自引:5,他引:4       下载免费PDF全文
Of the first 250 heart and 35 heart and lung transplant recipients at Papworth Hospital, Cambridge, who survived for more than one month after transplantation, 217 heart and 33 heart and lung patients were investigated serologically for evidence of Toxoplasma gondii infection. Six patients acquired primary T gondii infection, most probably from the donor organ. Five patients experienced T gondii recrudescence, two of whom had recovered from primary infection a few years earlier. Two patients died from primary T gondii infection and the severity of symptoms in the other patients with primary infection was related to the amount of immunosuppressive treatment. Prophylaxis with pyrimethamine (25 mg a day for six weeks) was introduced for T gondii antibody negative transplant recipients who received a heart from a T gondii antibody positive donor after the first four cases of primary toxoplasmosis. Of the seven patients not given pyrimethamine, four (57%) acquired primary T gondii infection. This compared with two of the 14 patients (14%) given prophylaxis.  相似文献   

12.
To examine the immunological changes in cats concurrently infected with feline immunodeficiency virus (FIV) and Toxoplasma gondii, kittens (four per group) were inoculated with FIV, T. gondii, both agents, or no pathogens. Blood mononuclear cells and plasma were collected weekly for lymphocyte assays and serology. At week 14, spleen and lymph node cells were used for lymphocyte assays; brains and mesenteric lymph nodes were used for isolation of T. gondii. More T. gondii organisms were present in tissues of the dually infected cats than in tissues of cats with toxoplasmosis alone. Two dually infected cats and one cat infected with T. gondii developed chorioretinitis. Spleen, lymph node, and blood mononuclear cells from dually infected cats had the greatest reduction in mitogenic responses. By week 3, cats infected with FIV underwent a decrease in the number of CD4 cells that was not changed by concurrent T. gondii infection; the number of CD8 cells increased only in cats infected with T. gondii alone. For cats infected with T. gondii, the responses of lymphocytes to T. gondii antigen were not affected by FIV infection; the responses to FIV antigen were negligible in all groups. Overall, this study indicates that FIV infection favors T. gondii proliferation. Also, the establishment of toxoplasmosis may enhance FIV-induced immunodeficiency and is likely to cause a more rapid disease progression than that from infection with FIV alone.  相似文献   

13.
Lymphocyte blastogenic transformation in response to Toxoplasma lysate antigen was markedly impaired in six of eight patients with chronic, latent Toxoplasma gondii infection and treated Hodgkin's disease. None of these patients with serum antibody to T. gondii measured by the Sabin Feldman Dye test and impaired lymphocyte transformation to T. gondii antigens had clinical or serologic evidence of disseminated, active infection with T. gondii. Partial depletion of adherent mononuclear leukocytes improved the impaired lymphocyte transformation of three of six patients; treatment of cultures from all patients with indomethacin improved their blastogenic transformation but culture with normal heterologous serum did not. These studies indicate that lymphocyte blastogenic response to T. gondii antigens is impaired in some patients with chronic, latent T. gondii infection and treated Hodgkin's disease but that this impairment of lymphocyte function is not sufficient to cause reactivation of chronic, latent T. gondii infection.  相似文献   

14.
An enzyme linked immunosorbent assay (ELISA) kit for detecting IgG class antibody to T gondii was compared with the latex agglutination test to determine the specificity as a screening method in 12 patients who had undergone heart transplantation (recrudescence of T gondii infection n = 3, donor acquired infection n = 3; acute cytomegalovirus infection n = 6). The latex agglutination test detected antibodies to primary T gondii infection much earlier in the infection than the ELISA, but the ELISA method was useful for detecting previous infection. It is concluded that the ELISA technique is more complex to perform than the latex agglutination test but the use of IgM and IgG assays combined could reduce the number of samples sent to the reference laboratory and thus reduce the time taken to obtain a final result.  相似文献   

15.
Han  Yajing  Nie  Lihong  Ye  Xiaohong  Zhou  Zixing  Huang  Shiqi  Zeng  Chengli  Guo  Congcong  Ou  Meiling  Xiao  Di  Zhang  Baohuan  Huang  Chuican  Ye  Xingguang  Jing  Chunxia  Yang  Guang 《Parasitology research》2018,117(3):689-695
Parasitology Research - Type 2 diabetes mellitus (T2DM) is a major global health problem. The rate of infection with Toxoplasma gondii (T. gondii) is more than one-third of the total world...  相似文献   

16.
Peroral infection of Toxoplasma gondii is thought to reflect the typical infection route of naturally acquired toxoplasmosis in humans. We have investigated possible differential roles of toll-like receptor 2 (TLR2) and TLR4 in host defense against naturally acquired murine toxoplasmosis. After peroral inoculation of T. gondii ME49 cysts, TLR4-deficient C3H/HeJ mice were more susceptible to infection than wild-type (WT) C3H/HeN mice, as shown by increased cyst number and low production of cytokines, which are the key factors in protective immunity. When mice were inoculated by intra-peritoneal inoculation of T. gondii, there were no significant differences in the number of brain cysts and cytokine productions between C3H/HeJ and C3H/HeN mice. Histopathologic examination revealed severe inflammation in the small intestine of C3H/HeJ (TLR4-deficient) mice, while an increased number of TLR4-positive mononuclear cells was found in C3H/HeN (WT) mice. To confirm these phenomena, TLR2(-/-) or TLR4(-/-) mice were infected perorally with T. gondii cysts. TLR4(-/-) mice were more susceptible to infection compared with TLR2(-/-) and C57BL/6 mice. Nuclear factor-kappa B activation through TLR4 agonistic activity of T. gondii ME49 was demonstrated by luciferase assay using stably expressing mouse (m) TLR2 or mTLR4/mMD-2 transfectants. We demonstrate here for the first time that innate immune recognition by TLR4 is involved in protective mechanisms against peroral infection with T. gondii ME49. These results suggest that the small intestine plays an important role in the induction of innate immunity in naturally acquired toxoplasmosis.  相似文献   

17.
弓形虫感染可激发宿主产生Th1型免疫反应,因此研究弓形虫疫苗尤其需要关注系统性T细胞免疫应答与保护力的关系。本研究被弓形虫Pru株包囊口服感染BALB/c小鼠,成功建立了弓形虫慢性感染小鼠模型。慢性感染小鼠的脾细胞用弓形虫可溶性抗原刺激后可以产生特异性增殖。进一步分析发现弓形虫特异性CD3+T、CD4+T和CD8+T细胞都可以产生IFN-γ。本研究建立的弓形虫慢性感染小鼠模型可以产生较强的系统性T细胞免疫应答,为弓形虫疫苗研制的效果评价提供重要参考。  相似文献   

18.
This paper describes the cloning, purification, and serological applications of matrix antigen MAG1 of Toxoplasma gondii. The expression system used allows the production of a large amount of T. gondii recombinant protein, which was assessed for its potential use in an enzyme-linked immunosorbent assay (ELISA) for detection of T. gondii infection in humans. Serum samples from 117 patients with different stages of infection, along with 10 serum samples from seronegative patients obtained for routine diagnostic tests, were used. The results were compared with those of an ELISA that uses a native T. gondii antigen extract. The MAG1 antigen detected antibodies more frequently from the acute stage (97.3%) than from the chronic stage (7.5%) of toxoplasmosis. Hence, this antigen may be used as a tool for detection of T. gondii immunoglobulin G antibodies in persons with acute toxoplasmosis.  相似文献   

19.
Resistance to Toxoplasma gondii involves the development of a highly polarized Th1-type cytokine expression. SAG1 transgenic mice are highly susceptible to T. gondii infection due to their non-reactivity to SAG1 of the protozoan parasite. Here we describe cytokine profiles during the acute phase of T. gondii infection, which are associated with the susceptibility of SAG1 transgenic mice. SAG1 transgenic mice showed a 4.5-fold increase in susceptibility upon inoculation with a sublethal dose of the Beverley strain of T. gondii compared to their wild-type counterparts (mortality: 81 vs. 18%, respectively). When analysis of the most important cytokines involved in the mediation of resistance to infection was carried out, SAG1 transgenic mice exhibited low production levels of IL-12, IFN-gamma and TNF-alpha in sera during the acute phase of T. gondii infection. Antibody and T cells specific for SAG1 were not mounted upon SAG1 stimulation in SAG1 transgenic mice. Moreover, in vitro studies indicated that in SAG1 transgenic mice IFN-gamma and IL-12 production was lower than in their wild-type counterparts, although levels of TNF-alpha increased in SAG1 transgenic mice on day 9 after infection. Low IgG2a levels were detected in SAG1 transgenic mouse sera. Unresponsiveness to SAG1 of T. gondii renders SAG1 transgenic mice unable to develop a strong Th1-based protection against T. gondii infection. These results provide evidence that SAG1 is a pivotal antigen involved in the induction of immune responses towards the development of Th1-protective immunity during T. gondii infection.  相似文献   

20.
Toxoplasma gondii is capable of invading and multiplying within murine peritoneal macrophages. Previous studies have shown that treatment of macrophage monolayers with recombinant gamma interferon but not tumor necrosis factor (TNF) is associated with intracellular killing of T. gondii by macrophages. Furthermore, infection of macrophages with T. gondii prevents their stimulation for mycobactericidal activity by TNF. Since transforming growth factor beta (TGF-beta) is known to suppress a number of functions in macrophages, we investigated the influence of infection with T. gondii on macrophage TNF receptors and on production of TGF-beta. Infection with T. gondii was associated with increased production of TGF-beta and downregulation of TNF receptors. This effect was observed early after infection and was partially inhibited by anti-TGF-beta 1 antibody.  相似文献   

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