共查询到10条相似文献,搜索用时 171 毫秒
1.
S. B. Cheknev 《Bulletin of experimental biology and medicine》1995,119(4):396-399
The proliferative activity of human natural killers (CD16+CD56+ cells) in the presence of 100 and 1000 IU/ml human recombinant interleukin-3 is investigatedin vitro. It is shown that recombinant interleukin-3 reliably enhances natural killer proliferation, causing a 9–15.2-fold increase
of3H-thymidine uptake by CD16+CD56+ cells both in complete culture medium and in conditioned medium. The effect of the factor is 3.9–6.4 and 3.6–8.9-fold more
potent than that of recombinant interleukin-2 and granulocyte-macrophage colony-stimulating factor, respectively, in the same
doses.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
4, pp. 409–412, April, 1995
Presented by S. V. Prozorovskii, Member of the Russian Academy of Medical Sciences 相似文献
2.
Lysis of pulmonary fibroblasts by lymphokine (IL-2)-activated killer cells—a mechanism affecting the human lung microenvironment? 下载免费PDF全文
R ZAMBELLO L TRENTIN C ENTHAMMER A CIPRIANI C AGOSTINI G SEMENZATO 《Clinical and experimental immunology》1996,105(2):383-388
In this study we investigated whether IL-2-activated killer cells may bind and exert lytic activity against non-transformed lung fibroblasts. We demonstrated that human lymphokine-activated killer (LAK) cells generated in vitro following incubation with recombinant IL-2 of either peripheral blood mononuclear cells (PB-LAK) or lymphocytes obtained from bronchoalveolar lavage (BAL-LAK), but not resting cells, can lyse normal lung fibroblasts obtained from transbronchial lung biopsies in a 4-h 51Cr release assay. Both autologous and allogeneic fibroblasts were consistently lysed by LAK cells, thus suggesting that the phenomenon we observed is not MHC-restricted. Since fibroblasts can bind IL-2 through specific receptors, we evaluated whether long-term culture with rIL-2 could modulate the susceptibility to lysis of target cells. Our data showed that autologous fibroblasts were more resistant to lysis than allogeneic fibroblasts when they were cultured with rIL-2. Since LAK cells have been demonstrated to release a series of different immunomodulatory cytokines, we evaluated the effect of short-term incubation of fibroblasts with different factors, including IL-1, IL-2, IL-3, IL-4, IL-6, tumour necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), on the binding and the lysis mediated by LAK cells. These cytokines were not directly cytotoxic on fibroblasts. Only IFN-γ was found to have a significant protective effect against the lysis. Our data support the concept that a self-directed cytotoxicity against pulmonary fibroblasts is generated during lymphocyte activation with rIL-2. 相似文献
3.
V. L. Nazarova E. R. Andreeva V. V. Tertov B. S. Gel'dieva A. N. Orekhov 《Bulletin of experimental biology and medicine》1995,120(2):839-842
Scavenger receptor was soughtin situ in human aortic smooth-muscle cells and in a primary culture of intact human aortic intima using antibodies to scavenger
receptor. For identification of smooth-muscle cells, double staining making use of antibodies to murine α-actin was used.
The presence of scavenger receptor in smooth-muscle cells of the intima and media of human aorta was demonstrated on aortic
slices. In cultured smooth-muscle cells from normal human aortic intima scavenger receptor was distributed over the entire
surface of the cell membrane, forming clusters in some places. These results suggest that human aortic smooth-muscle cells
express scavenger receptor.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
8, pp. 195–198, August, 1995
Presented by V. N. Smirnov, Member of the Russian Academy of Medical Sciences 相似文献
4.
E. Yu. Gusev N. N. Kevorkov V. L. Ponosov 《Bulletin of experimental biology and medicine》1994,118(5):1196-1198
CBA, CC57BR, C57B1/6, BALB/c, and outbred white mice were intraperitoneally or subcutaneously (C57B1/6 strain) immunized with
sheep red cells in a dose optimal for the development of delayed-type hypersensitivity but subthreshold for antibody production.
Seven days later the mice were reimmunized with sheep red cells in various doses subcutaneously (CBA, C57B1/6, BALB/c, outbred
mice) or intraperitoneally (CBA, CC57BR, outbred mice), and 5 days after reimmunization the intensity of antibody production
and delayed-type hypersensitivity was assessed. Intact mice were controls. The immunization was found to selectively enhance
delayed-type hypersensitivity in C57B1/6, CC57BR, and BALB/c mice and to intensify antibody production in CBA mice; both phenomena
were observed in outbred mice.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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11, pp. 499–501, November, 1994
Presented by K. P. Kashkin, Member of the Russian Academy of Medical Sciences 相似文献
5.
A. D. Mikhailov A. A. Malakhov E. S. Revazova T. I. Valyakina T. V. Yudicheva 《Bulletin of experimental biology and medicine》1995,119(2):201-203
Two human melanoma cell variats-with low and high metastasizing activity-are obtained by successive passaging on mice with
combined immunodeficiency. After the development of a subcutaneous tumor, tumor cells are detected only in the bloodstream
of animals with a highly metastasizing tumor, in mice with combined immunodeficiency the number of these cells being much
greater than that in nude mice. These results indicate a preeminent influence of the nature of tumor cells on the dissemination
of metastasizing cells.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
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2, pp. 206–208, February, 1995
Presented by Yu. N. Solov'ev. Member of the Russian Academy of Medical Sciences. 相似文献
6.
N. B. Dolzhanskaya A. L. Shvartsman V. S. Gaitskhoki 《Bulletin of experimental biology and medicine》1994,117(2):166-167
A genetic-engineering construction is developed containing the full-size cDNA of human α-1-antitrypsin, controlled by the
promotor and enhancer elements from cytomegalovirus. It is shown that, after transfection with this recombinant DNA, it is
properly expressed in heterologous animal cells.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, vol. 117, N
o
2, pp. 166–167, Feburary, 1993
Presented by A. N. Klimov, Member of the Russian Academy of Medical Sciences 相似文献
7.
A. L. Pukhal'skii A. P. Toptygina E. A. Kalashnikova S. D. Shiyan V. V. Nasonov N. V. Bovin A. G. Lyutov F. T. Bairushin 《Bulletin of experimental biology and medicine》1994,118(1):755-757
The mechanisms of the antiproliferative effect of α1-acid glycoprotein (AGP) isolated from the blood of healthy donors (nAGP) and from the ascitic fluid of patients with stomach
cancer (aAGP) are studied. Three fractions of AGP are divided into 3 groups according to their ability to bind to concanavalin
A (ConA): AGP not binding to ConA (AGP-1) and AGP weakly (AGP-2) and strongly (AGP-3) binding to ConA. It is shown that native
preparation of aAGP has a more potent inhibitory effect on lymphocyte proliferation than native preparation of nAGP. The most
potent inhibitory effect is exerted by AGP-3. Native preparation of aAGP does not affect the secretion of interleukin-2 (IL-2)
by lymphocytes, whereas AGP-1 inhibits this process. The weakly bound fraction has a stimulatory effect both on the proliferative
response and on IL-2 secretion.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
7, pp. 71–73, July, 1994 相似文献
8.
I. S. Komolov A. A. Bulatov E. E. Makarovskaya G. P. Elizarova S. Yu. Serpukhovitin S. Yu. Kasumova E. I. Marova 《Bulletin of experimental biology and medicine》1994,118(5):1241-1244
Some features of the morphological cellular structure of prolactin secreting human pituitary adenomas and their secretion
of prolactin and somatotropic hormone in primary suspension cultures were investigated. A possiblein vitro proliferation of lactotrophs was established. The inhibitory effect of somatostatin and its synthetic analog sandostatin,
on prolactin secretion in prolactinomas was found to be less than in somatotropic hormone-secreting pituitary tumors.
Presented by A. N. Konovalov, Member of the Russian Academy of Medical Sciences
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
11, pp. 543–546, November, 1994 相似文献
9.
F. V. Donenko S. M. Sitdikova A. O. Kabieva B. E. Polotskii Z. O. Machaladze M. I. Davydov L. V. Moroz 《Bulletin of experimental biology and medicine》1995,119(2):206-208
Preincubation of cells of BDF1 hybrid mice with P388 leukemia with doxorubicin and buthionine sulfoximine leads to the manifestation
of a therapeutic effect of the antibiotic. Injection of buthionine sulfoximine and ethacrinic acid to mice with leukemia does
not alter the therapeutic effect of the antibiotic.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
2, pp. 212–214, February, 1995
Presented by N. N. Trapeznikov, Member of the Russian Academy of Medical Sciences 相似文献
10.
M. S. Pokrovskaya L. K. Romanova T. B. Mladkovskaya G. V. Kulikova 《Bulletin of experimental biology and medicine》1994,118(6):1337-1339
Different doses of the isobutyl ester of retinoic acid were administered intraperitoneally into rats for two weeks. The dose
of 1 mg/kg caused a significant increase in the mitotic activity of cells of the macrophagal series in alveoli; an anti-inflammatory
effect was observed at a dose of 10 mg/kg. The isobutyl ester of retinoic acid did not affect the cell ratio (alveolar macrophages,
lymphocytes, neutrophils, eosinophils) in the internal medium of the lungs.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
12, pp. 645–647, December, 1994
Presented by N. K. Permyakov, Member of the Russian Academy of Medical Sciences 相似文献