新生儿窒息血清肌酸激酶、肌酸激酶同工酶、肌钙蛋白I和肌红蛋白检测的临床意义

目的 探讨新生儿窒息后肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、肌钙蛋白I( cTnI)和肌红蛋白在心肌损伤早期诊断的临床意义.方法 采用免疫化学发光法和酶动力学法来对60例窒息新生儿及30例健康足月新生儿进行CK、CK-MB、cTnI和Mb检测.结果 窒息新生儿组CK、CK-MB、cTnI和Mb值与健康新生儿组比较均显著升高(P＜0.01),且随窒息程度加重而升高.结论 窒息对新生儿心肌产生损伤,CK、CK-MB、cTnI和Mb能较好地反映心肌损害程度.     

外周血 cTnI 联合心肌酶谱检测在老年急性心肌梗死诊断中的应用研究

目的:分析外周血cTnI联合心肌酶谱检测在老年急性心肌梗死诊断中的应用价值.方法:回顾性分析我院2019年4月至2020年8月收治的62例老年急性心肌梗死(研究组)患者临床资料;另选取同期于本院进行健康体检者62例(对照组).比较两组肌钙蛋白I(Cardiac troponin I,cTnI),心肌酶四项[包括乳酸脱氢酶(Lactate dehydrogenase,LDH)、谷草转氨酶(Aspartate transaminase,AST)、肌酸激酶(Creatine Kinase,CK)以及肌酸激酶同工酶(creatine kinase-MB,CK-MB)]的水平,并分析cTnI、心肌酶谱四项检测及两者联合检测诊断急性心肌梗死的敏感性与特异性.结果:研究组血清cTnI、LDH、AST、CK、CK-MB水平均高于对照组(P<0.05).cTnI联合心肌酶谱四项检测敏感性及特异性均高于单一cTnI、心肌酶谱检测(P<0.05).结论:心肌酶谱与cTnI联合诊断急性心肌梗死敏感性、特异性更高,可有效降低漏诊和误诊的风险.     

新生儿缺氧缺血性脑病血清心肌肌钙蛋白Ⅰ及肌酸激酶检测的研究

目的　探讨血清心肌肌钙蛋白Ⅰ (cTnI)在新生儿缺氧缺血性脑病 (HIE)时心肌损伤的诊断价值。方法　检测4 5例HIE患儿的血清心肌肌钙蛋白I(cTnI)和肌酸激酶同工酶 (CK MB) ,并同时检测 30例健康儿童作为对照组。结果　HIE患儿血清cTnI和CK MB明显高于正常对照组 (P <0 .0 1,P <0 .0 5 ) ,血清cTnI在诊断HIE患儿心肌损伤时较血清CK MB更为敏感 (P <0 .0 5 ) ;结论　HIE患儿血清cTnI和CK MB明显高于正常小儿 ,说明HIE患儿存在心肌损伤 ,血清cTnI在诊断心肌损伤时较血清CK MB更为敏感。     

cTnI、CK-MB在AMI早期诊断中的临床应用

目的 :观察急性心肌梗死 (Acutemyocardialinfarction ,AMI)患者早期诊断指标心肌肌钙蛋白I(cardiactrofoninI,cTnI)与肌酸激酶同工酶MB(CreatineKinase MB ,CK MB)的水平 ,为AMI的诊断、预后及疗效判断提供可靠的参考依据。方法 :选用AMI患者 82例 ,不稳定性心绞痛 (UnstableAngina,UA)患者 30例 ,稳定性心绞痛患者 (StableAngina,SA) 2 2例。所有血清标本均采用化学发光免疫分析技术在Bayer公司的ACS:180系统上检测cTnI、CK MB的含量 ,并比较两者诊断AMI的准确性以及在体内的时间动态变化。结果 :AMI组患者血清中cTnI和CK MB同时增高 ,在 3组患者中cTnI诊断AMI的准确性为98.8% ,CK MB的准确性为 82 .8% ,两者有显著性差异 (P <0 .0 5 )。CTnI血清水平在第 4天仍维持在正常值以上 ,而CK MB在第三天就已全部恢复正常 ,cTnI持续时间较CK MB长 (P <0 .0 5 )。结论 :cTnI和CK MB联合应用可以提高AMI的诊断率 ,减少假阳性 ,对于AMI的病情变化也有一定的预示作用     

cTnI快速定量检测在诊断非ST段抬高型急性心肌梗死中的应用

目的:探讨肌钙蛋白I(cTnI)快速定量检测在诊断非ST段抬高型急性心肌梗死(NSTEMI)中的优越性,进一步推广在基层医院临床中的应用.方法:选取住院怀疑为NSTEMI的85例患者作为对象,抽取入院即刻、24h血清,同步检测cTnI、肌酸激酶同工酶(CK-MB),结合临床、心电图对NSTEMI作出诊断,对两种方法的差异进行统计学分析.结果:NSTEMI患者早期cTnI的阳性率明显高于CK-MB,cTnI在诊断NSTEMI上具有简便快速,出现时间早,诊断窗口时间长等优点,可有效防止漏诊.结论:cTnI快速定量检测方法简便,敏感性高、特异性强,在诊断NSTEMI中具有重要意义,可在基层医院中推广应用.     

心肌损伤标志物检测方法进展

心肌损伤标志物包括心肌肌钙蛋白Ⅰ(cTnI)、肌红蛋白(Mb)和肌酸激酶同工酶(CK-MB)等.在心肌损伤相关疾病的诊断上,cTnI特异性强,Mb和CK-MB早期敏感性高,三者联合检测可提高诊断的特异性、敏感性,临床广泛应用于心肌梗死和心绞痛的诊断、危险性估计和预后判断,心肌梗死后临床溶栓治疗效果判定,诊断各种心肌损伤和判断心肌缺血损伤面积,判断左心室功能以及某些治疗药物的l临床疗效观察等.     

cTnI快速定量检测在诊断非ST段抬高型急性心肌梗死中的应用

目的:探讨肌钙蛋白I(cTnI)快速定量检测在诊断非ST段抬高型急性心肌梗死(NSTEMI)中的优越性,进一步推广在基层医院临床中的应用.方法:选取住院怀疑为NSTEMI的85例患者作为对象,抽取入院即刻、24h血清,同步检测cTnI、肌酸激酶同工酶(CK-MB),结合临床、心电图对NSTEMI作出诊断,对两种方法的差...     

血浆cTnI、CK-MB及BNP水平在急性胸痛患者中的变化及临床意义

目的探究血浆心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)、脑钠肽(BNP)水平在急性胸痛患者中的变化及临床意义。方法选择2016年1月至2019年1月我院收治的以急性胸痛为首发症状的患者作为研究对象,其中急性心肌梗死(AMI)患者59例,不稳定心绞痛(UAP)患者42例,稳定型心绞痛(SAP)患者38例,另39例患者经检查冠脉未见明显异常,纳入对照组。收集患者临床资料,采用GRACE评分工具进行危险分级,测定血浆cTnI、CK-MB及BNP水平。结果SAP、UAP、AMI组患者高脂血症患病率显著高于对照组(P<0.05),左心射血分数(LVEF)显著低于对照组(P<0.05),血浆cTnI、CK-MB、BNP水平高于对照组(P<0.05);ROC曲线示,血浆cTnI、CK-MB、BNP指标诊断心源性胸痛的曲线下面积分别为0.834、0.792、0.892,各指标联合诊断的曲线下面积为0.935;随着危险程度的升高,血浆cTnI、CK-MB、BNP水平升高(P<0.05);Pearson相关性分析示,血浆cTnI、CK-MB、BNP与LVEF呈负相关(P<0.05),与冠脉病变支数、GRACE评分呈正相关(P<0.05)。结论心源性胸痛患者可出现血浆cTnI、CK-MB、BNP等水平的升高,且升高幅度与病情严重程度相关,临床上可将其作为诊断高危性缺血性疾病的参考指标。     

肌红蛋白、心肌肌钙蛋白和肌酸激酶同工酶检测对新生儿窒息心肌损害的诊断价值

探讨肌红蛋白(Mb)、心肌肌钙蛋白(cTnI)和肌酸激酶同工酶(CK-MB)测定在诊治窒息新生儿心肌损害的价值.采用免疫化学发光法和酶动学法对35名正常足月新生儿、50例窒息新生儿进行Mb、cTnI和CK-MB检测.结果表明: 与正常新生儿比较, 患儿组Mb、cTnI和CK-MB值均显著升高(P＜0.01),且随窒息程度加重而升高, 其中Mb灵敏度最高, cTnI特异性最强.研究表明: 新生儿窒息对患儿心肌功能产生损伤, Mb、cTnI和CK-MB三项指标能较好反映心肌损害程度.     

三种常见精神疾病心肌酶及心电图对比分析

目的：对比不同精神科疾病患者心肌酶及心电图情况,探讨心肌酶变化在不同精神科疾病患者诊断治疗方面的意义.方法：通过随机抽取既往1年住我院某病区的精神分裂症患者、双相情感障碍-躁狂发作、躁狂发作、双相情感障碍-抑郁发作、抑郁发作患者病历,对其入院次日清晨心肌酶检测结果,包括肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）的水平以及患者的心电图情况进行对比分析.结果：精神分裂症、躁狂发作及双相障碍-躁狂发作患者的心肌酶及心率有不同程度升高,并与抑郁发作和双相障碍-抑郁发作患者对比有统计学意义.结论：心肌酶的增高对于精神疾病诊断有一定临床参考意义.     

Recovery of responsiveness of cells of lateral geniculate nucleus of rat

1. Recovery of responsiveness of single cells in lateral geniculate nucleus of rat has been determined in both P and I cells. There are three types of recovery curve among P cells; (a) early recovery, (b) early partial recovery followed by depression and then complete recovery, (c) prolonged depression followed by cyclic recovery. Type (c) is by far the commonest recovery curve. In contrast to the spike in a P cell, the synaptic potential recovers to its full amplitude in about 20 msec. All I cells exhibit similar rapid recovery curves after a prolonged depression.2. Conditioning stimuli applied to visual cortex also produce a prolonged depression in most P cells but I cells can be re-excited at short intervals from cortex. Decortication does not prevent the prolonged depression of the multineuronal response produced by optic nerve stimulation.3. A neuronal model is proposed to explain these observations. It is supposed that I cells (interneurones) are innervated by axon collaterals of the P cells (principal cells, projecting to visual cortex) and that the I cells exert an inhibitory influence on the P cells.     

Modes of Inheritance of Errors of Refraction

Eighteen families in which both parents had refractions within the range of +4·0 D to −4·0 D and axial lengths seen in emmetropia (22·3-26·0 mm) showed coefficients of correlation of the order 0·5 indicative of polygenic inheritance. Such coefficients were seen for axial length (0·407) and for the cornea (0·487), but not for the lens (which is known to be yoked to the axial length). No such coefficients were seen in 19 families in which one of the parents had axial length outside the emmetropic range (nine families with long axes and 10 with short axes).

The pattern of polygenic inheritance for emmetropia (completely correlated optical components) and errors of refraction up to 4·0 D (inadequately correlated components: correlation ametropia) follows that seen in stature and other measurable characters. In contrast the high refractive errors with their abnormal axial lengths (component ametropia) are—like the extremes in stature—pathological anomalies with monofactorial inheritance.

     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

Analysis of two types of dopaminergic responses of neurons of the spinal ganglia of rats

It was established, in experiments on isolated spinal ganglia of adult rats in concluons of intracellular recording, that dopamine (1 M/liter) elicits depolarized responses in 61% of neurons, hyperpolarized in 20% of neurons, and depolarized-hyperpolarized in 19% of neurons. The depolarized responses are associated with the activation of D₁ dopamine receptors, and are governed by the shift of cAMP-dependent cation (sodium) channels to the conducting state. The hyperpolarized responses are triggered by the activation of D₂ dopamine receptors, which by means of HTP-binding protein convert the potassium channels to the conducting state. The change in the polarization of neurons with the action of dopamine influences their electrical excitability variously.Translated from Fiziologicheskii Zhurnal SSSR imeni I. M. Sechenova, Vol. 76, No. 6, pp. 739–745, June, 1990.