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1.
Xiaoli Shi Ming Zou Jing He Hai Xie Xu Li 《African journal of traditional, complementary, and alternative medicines》2014,11(2):334-338
Background
The main functions of Radix Glycyrrhizae include regulating middle warmer, moistening lung, relieving toxicity, harmonizing property of drugs which is a traditional Chinese medicine widely used in clinical settings. The objective of the paper is to isolate and identify the constituents in ethanol extract of Radix Glycyrrhizae, and to study their anticancer activity.Materials and Methods
Column chromatography, ODS column chromatography, preparative thin layer chromatography and NMR spectroscopy techniques were used to isolate compounds from ethanol extract of Radix Glycyrrhizae; optical microscopy and flow cytometry were used to determine the anticancer effect of Radix Glycyrrhizae extract.Results
Four compounds were isolated from the ethanol extract of Radix Glycyrrhizae, namely oleanolic acid, isoliquiritin, glycyrrhetinic acid and licochalcone A. Optical microscopic observation showed that the growth of gastric cancer cell line SGC-7901 was inhibited in the experimental groups, and apoptotic morphological changes were observed in adherent cells; flow cytometry with PI staining showed that Radix Glycyrrhizae extract could induce SGC-7901 cell apoptosis within a concentration range of 0.5–1.5 mg/mL, compared with the control group, the apoptosis was positively correlated with the drug concentration, which exhibited an apparent dose-dependence.Conclusion
We conclude Ethanol extract of Radix Glycyrrhizae has an anti-proliferative activity on SGC-7901 cells. 相似文献2.
Background
Radix astragali mainly contains saponins, polysaccharides, flavonoids, amino acids and other chemical constituents of which total astragalosides have immunomodulatory, anti-viral, hepatoprotective, and gastric mucosa protective effects.Objective
To investigate the process conditions for extraction, purification and enrichment of total astragalosides by macroporous adsorption resin, and to study the inhibitory effect of total astragalosides on growth of human gastric cancer cell line MKN-74.Methods
UV spectrophotometry was applied to determine the adsorption and desorption capacity of macroporous adsorption resin on total astragaloside content, MTT assay was used to determine the inhibition of MKN-74 cell growth by total astragalosides.Results
The dynamic adsorption performance of DA201 adsorption resin was examined, and the dynamic adsorption curve of total astragalosides on DA201 resin column was plotted. Meanwhile, eluent and elution flow rate were investigated, the results showed that the choice of eluent of 80% ethanol, and a flow rate of 5 BV/h could maximize the yield of total astragalosides. MTT assay found that astragalosides could relatively pronouncedly inhibit the proliferation of MKN-74 cells, and the inhibitory effect was enhanced with the increase of astragaloside dose and the extension of processing time, which showed a dose-and time-dependence.Conclusion
DA201 resin can effectively enrich total astragalosides, total astragalosides have an inhibitory effect on growth of MKN-74 cells. 相似文献3.
Xiang Lu Bin Lin Jia-guang Tang Zheng Cao Yuan Hu 《African journal of traditional, complementary, and alternative medicines》2014,11(1):172-175
Background
Ku Dou Zi is the dried whole plant, roots and seeds of Sophora alopecuroides L. in the genus Sophora of family Leguminosae. The entire plant is bitter in taste, cold in nature, and has the heat clearing, detoxifying, pathogenic wind dispelling dampness, analgesic, and insecticidal effects. Modern pharmacological studies have proved that TASA has pharmacological activities of anti-cancer. The objective of this paper was to investigate the inhibitory effect of total alkaloids of Sophora alopecuroides (TASA), on osteosarcoma cell growth and its mechanism.Materials and Methods
MTT assay and flow cytometry were used to study the inhibitory effect of TASA on human osteosarcoma cell line OS732.Results
The results showed that the inhibition rates of different concentrations of TASA (1.5, 3, and 4.5g/kg), against human osteosarcoma cell line OS732, were: 18.4%, 27.4% and 52.8%, respectively.Conclusion
TASA has an inhibitory effect on osteosarcoma cell growth. 相似文献4.
Kebin Zheng Chunhui Li Xiaosong Shan Haipeng Liu Wufang fan Zhenshan Wang 《African journal of traditional, complementary, and alternative medicines》2014,11(1):156-160
Background
Sophora flavescens Ait. is a traditional Chinese medicine with a long history in China. It is mainly used in the treatment of heat dysentery and similar ailments in the clinical. The objective of this paper was to isolate, purify and identify alkaloids from Sophora flavescens Ait. and to explore their inhibitory effects on C6 glioma cells.Materials and Methods
Column chromatography, extraction and NMR spectroscopy were used to structurally identify the isolated compounds. MTT assay and flow cytometry were used to detect the inhibitory effect of matrine on C6 cells.Results
Three compounds were isolated from Sophora flavescens Ait., namely matrine, oxymatrine and lupeol. Different concentrations of matrine solution all had inhibitory effects on growth of C6 cell lines, which showed apparent dose-effect relationship. Compared with the control group, proportion of G0/G1 phase cells increased in each matrine concentration group to a maximum of 79.8%; proportion of S phase cells reduced, and proportion of G2/M phase cells declined slightly to a minimum of 6.3%, suggesting that after the action of matrine proliferation of C6 cells was significantly inhibited and the cells were arrested in the G1 phase.Conclusion
We concluded that Sophora flavescens Ait. has an inhibitory effect on C6 cell proliferation. 相似文献5.
Background
Surgery has been reported to suppress cell-mediated immunity; however, the detailed mechanisms responsible for this remain unclear. This study determined the expression of lymphocyte activation gene 3 (LAG-3) and programmed cell death 1 (PD-1) in lymphocytes following surgery for gastric cancer.Methods
LAG-3 and PD-1 expression on both CD4+ and CD8+ T cells obtained pre- and post-operatively from gastric cancer patients were evaluated by multicolor flow cytometry.Results
The total lymphocyte count decreased rapidly from preoperative levels, reaching a minimum on postoperative day 1 and remaining significantly decreased on days 3 and 7. PD-1+CD4+ T cells significantly increased, reaching a maximum on postoperative day 1 and remaining significantly elevated on day 3. PD-1+CD8+ T cells significantly increased and reached a maximum on day 7 before returning to the preoperative level on day 30. There were no statistically significant differences in the frequency of LAG-3+CD4+ or LAG-3+CD8+ T cells after surgery. There were significant positive correlations between PD-1 and LAG-3 expression on both CD4+ andCD8+ T cells.Conclusion
PD-1 and LAG-3 expression on both CD4+ and CD8+ T cells was up-regulated and might be related to impaired cell-mediated immunity after surgery for gastric cancer. 相似文献6.
Yoon Young Choi Ji Yeong An In Cho In Gyu Kwon Dae Ryong Kang Woo Jin Hyung Sung Hoon Noh 《Yonsei medical journal》2014,55(1):61-69
Purpose
To identify the effect of insufficient lymph node dissection (LND) on the survival of patients with pT2 gastric cancer.Materials and Methods
A total of 340 patients (120 patients with insufficient LND and others with D2 LND) who underwent gastrectomy for pT2 gastric cancer between January 2008 and December 2010 were included.Results
The incidence of preoperatively diagnosed early gastric cancer was higher and there were fewer metastatic lymph nodes (LNs) in the insufficient LND group than the D2 group, but there was no survival difference between two groups (p=0.365). Among the 89 patients with metastatic LNs after D2 LND, 13 patients (14.6%) had metastatic LNs at selected N2 stations (#10, 11, or 12a), but none of these patients were in the pN1 category. One patient had five metastatic LNs at station #11p with no metastatic LNs at any other stations. The number of metastatic LNs was identified as the only risk factor for LN metastasis at selected N2 stations by logistic regression.Conclusion
If a patient has pN0 or pN1 category after insufficient LND for pT2 gastric cancer, the surgery can be regarded as secure. However, for patients with pN2 or pN3 category, more careful examination is required. 相似文献7.
Shi-Qi Wen Quan Chen Ming Hu 《African journal of traditional, complementary, and alternative medicines》2014,11(1):131-134
Backgroud
Cantharidin, and its derivatives can not only inhibit the proliferation of tumor cells, but can also induce tumor cell apoptosis. It shows cantharidin exhibits a wide range of reactivity in anticancer. The objective of this paper was to study the inhibitory effect of sodium cantharidinate on human hepatoma HepG2 cells.Materials and Methods
MTT assay was used to detect the proliferation of HepG2 cells, and immunohisto-chemical method was used to detect the change in VEGF, protein level, and to determine the inhibitory effect of sodium cantharidinate on human hepatoma HepG2 cells.Results
As results, sodium cantharidinate significantly inhibited the growth of HepG2 cells in a time-and dose-dependent manner.Conclusion
We conclude that sodium cantharidinate has an inhibitory effect on human hepatoma HepG2 cells. 相似文献8.
W Li D Wu B Wei S Wang HX Sun XL Li F Zhang CL Zhang Y Xin 《African journal of traditional, complementary, and alternative medicines》2014,11(5):99-104
Background
Cactus polysaccharides are the active components of Opuntia dillenii which have been used extensively in folk medicine. In this study, we investigate the anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells SK-MES-1.Materials and Methods
The inhibitory effect of Cactus polysaccharides on lung squamous carcinoma cells were detected by MTT assay. Cell cycle was determined by flow cytometry and cell apoptosis was determined by AnnexinV assay. Western-blotting was applied to detect P53 and PTEN protein expression in the cells treated with cactus polysaccharides.Results
Results showed that different concentrations of wild cactus polysaccharides prevent SK-MES-1 cells growth and induces S phase arrest. The data also revealed that cactus polysaccharides cause apoptosis in SK-MES-1 cells determined by Annexin-V assay. Furthermore, cactus polysaccharides induced growth arrest and apoptosis may be due to the increase of P53 and phosphatase and tension homolog deleted on chromosome ten (PTEN) protein.Conclusion
Cactus polysaccharides have anti-tumor activity on lung squamous carcinoma cells. 相似文献9.
Study Objectives:
Using a comprehensive cognitive-behavioral model of insomnia and a daily process approach, this study was conducted to examine the contribution of cancer symptoms and dysfunctional sleep related thoughts and behaviors to the process of insomnia in breast cancer survivors.Design:
Within-group longitudinal research design.Setting:
An academic medical center.Participants:
41 women with breast cancer who had completed their primary cancer treatment and met Research Diagnostic Criteria for primary insomnia or insomnia comorbid with breast cancer.Interventions:
NAMeasurements and Results:
For 28 days, participants completed morning diaries assessing sleep, nighttime pain and hot flashes, and dysfunctional sleep related thoughts and behaviors during the day and night, and evening diaries assessing daytime pain, fatigue, hot flashes, and mood. All diaries were collected using an automated telephone-based system. Results revealed that poorer sleep was related to nighttime pain and hot flashes in breast cancer patients. Time-lagged effects were also found. The current study identified higher levels of dysfunctional sleep related thoughts and sleep inhibitory behaviors during the day and night as antecedents of insomnia, and higher levels of pain, fatigue, and hot flashes and lower levels of positive mood and dysfunctional sleep related thoughts as consequences of insomnia in this population.Conclusions:
The current study found support for a comprehensive cognitive-behavioral model of insomnia, which has several theoretical, practice, and research implications.Citation:
Rumble ME; Keefe FJ; Edinger JD; Affleck G; Marcom PK; Shaw HS. Contribution of cancer symptoms, dysfunctional sleep related thoughts, and sleep inhibitory behaviors to the insomnia process in breast cancer survivors: a daily process analysis. SLEEP 2010;33(11):1501-1509. 相似文献10.
Nianyu Xue Pintong Huang Wilbert S. Aronow Zongmin Wang Chandra K. Nair Zhiqiang Zheng Xuedong Shen Yimei Yin Fuguang Huang David Cosgrove 《Archives of Medical Science》2011,7(3):457-464
Introduction
Double contrast-enhanced ultrasonography (DCUS) is a new method we used in predicting lymph node metastasis (LNM) in patients with early gastric cancer.Material and methods
Seventy-six patients with early gastric cancer diagnosed by gastroscope and confirmed by pathology after operation were examined using DCUS preoperatively. Group N1 included 15 patients with LNM and group N0 61 patients without LNM.Results
In group N1, 13 patients (87%) had marked hyperenhancement during early arterial phase using DCUS, and 2 patients (13%) were unmarked as hyperenhancement. In group N0, 24 patients (39%) had marked hyperenhancement during early arterial phase using DCUS, and 37 patients (61%) had unmarked hyperenhancement. The sensitivity and specificity of marked hyperenhancement in predicting LNM in patients with early gastric cancer was 86.7% and 60.7% respectively, and the Youden’s index was 0.474. The κ value of this method was 0.89.Conclusions
Double contrast-enhanced ultrasonography is a new valuable method to evaluate LNM at an early stage of gastric cancer and prognosis of early gastric cancer preoperatively. 相似文献11.
Joji Watanabe Hiroaki Saito Kozo Miyatani Masahide Ikeguchi Yoshihisa Umekita 《Yonago acta medica》2015,58(3):137-143
Background
Thymic stromal lymphopoietin (TSLP) plays an important role in promoting tumor survival, by manipulating the immune response and angiogenesis. However, the clinical significance of TSLP in gastric cancer is unclear.Methods
Immunohistochemistry was used to investigate TSLP expression in non-cancerous gastric mucosa and gastric cancer tissue from patients with gastric cancer. Serum TSLP levels were measured using an enzyme-linked immunosorbent assay.Results
Tumors with TSLP expression were significantly larger than those without TSLP expression. TSLP expression was observed more frequently in advanced (T2/T3/T4) than in early (T1) gastric cancer and in stage 3/4 than in stage 1/2. Lymph node metastasis, liver metastasis, positive peritoneal lavage cytology, lymphatic invasion, and vascular invasion occurred significantly more often in TSLP-expressing than in non-expressing tumors. The prognosis of patients with TSLP-positive tumors was significantly worse than that of patients with TSLP-negative tumors. Patients with high serum TSLP concentrations also had a significantly worse prognosis than those with low concentrations. Multivariate analysis identified serum TSLP level as an independent prognostic indicator.Conclusion
TSLP is closely related to the progression of gastric cancer and may predict survival in these patients. 相似文献12.
13.
Ko-Nien Shih Wen-Tao Huang Chung-Liang Chang Chih-Chung Feng 《African journal of traditional, complementary, and alternative medicines》2014,11(1):187-193
Background
Hepatitis B virus (HBV) infection and hepatocellular carcinoma are major diseases that affect the Taiwanese population. Therefore, the development of an alternative herbal medicine that can effectively treat these diseases is a research target. In this study, we tested Ixeris Chinensis (Thunb.) Nakai boiling water extract (ICTN BWE) in vitro and analysed its effects on the HBV and liver cancer.Materials and Methods
We used a human liver cancer cell line (Hep3B, a cell line that continuously secretes HBV particles into a medium) as an experimental model for the screening of various ICTN BWE concentrations and their effects on the HBV in vitro.Results
Our results showed that 75 µg/mL ICTN BWE downregulated the relative expression of the hepatitis B virus surface antigens (HBsAg) to 77.1%. Using the human liver cancer cell lines HuH-7 and HepG2, and 3-(4,5-dimethylthiazol-zyl)-2,5-diphenyl tetrazolium bromide (MTT) and tumour clonogenic assays, we then showed that ICTN BWE inhibits hepatocellular carcinoma growth.Conclusion
Fluorescent microscopy of DAPI(4′,6-Diamidino-2-phenylindole)-stained nuclei and DNA fragmentation assays confirmed the inhibitory effects of ICTN BWE on liver tumour cell growth through induction of apoptosis. 相似文献14.
Kui Zhao Bao-Song Zhu Wei Gong Mo-Li Zhu Zhi-Tao Gao Yong-You Wu Qiang Chen Xiao-Dong Yang Chun-Gen Xing 《Archives of Medical Science》2013,9(6):990-998
Introduction
In the previous study, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) by induced SGC7901 cell death in vitro. We did not know whether SN50, which is a specific inhibitor of nuclear factor κB (NF-κB), could increase the cell death induction of gastric cancer of LY294002 in vitro, and we also wanted to know the mechanism of it, which might be applied to clinical tumor therapy. LY294002Material and methods
The 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxic effects of the drugs. Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Hoechst 33258 staining was used to detect apoptosis and necrosis morphological changes after and/or SN50 treatment. Expression of p53, PUMA and Beclin1 were determined with real-time polymerase chain reaction (RT-PCR) analysis. We used transmission electron microscopy to identify ultrastructural changes in SGC7901 cells after LY294002 and/or SN50 treatment. LY294002Results
In this study, we found that treating the human gastric cancer cells SGC7901 with SN50 could significantly enhance the effects of on inducing cell death after 24 h, compared to the control group (p < 0.05). Detection of mitochondrial potential and transmission electron microscopic examination indicated that the rate of cell death increased progressively. The expression of p53, PUMA and Beclin1 was up-regulated. LY294002Conclusions
The NF-κB inhibitor SN50 could enhance the role of on inducing cell death of human gastric cancer cells SGC7901, which might be a promising new approach to gastric cancer therapy. LY294002相似文献15.
Sima Ferman Marceli de Oliveira Santos Juliana Moreira de Oliveira Ferreira Rejane de Souza Reis Julio Fernando Pinto Oliveira Maria S. Pombo-de-Oliveira Beatriz de Camargo 《Clinics (S?o Paulo, Brazil)》2013,68(2):219-224
OBJECTIVES:
Childhood cancer mortality has substantially declined worldwide as a result of significant advances in global cancer care. Because limited information is available in Brazil, we analyzed trends in childhood cancer mortality in five Brazilian regions over 29 years.METHODS:
Data from children 0-14 years old were extracted from the Health Mortality Information System for 1979 through 2008. Age-adjusted mortality rates, crude mortality rates, and age-specific mortality rates by geographic region of Brazil and for the entire country were analyzed for all cancers and leukemia. Mortality trends were evaluated for all childhood cancers and leukemia using joinpoint regression.RESULTS:
Mortality declined significantly for the entire period (1979-2008) for children with leukemia. Childhood cancer mortality rates declined in the South and Southeast, remained stable in the Middle West, and increased in the North and Northeast. Although the mortality rates did not unilaterally decrease in all regions, the age-adjusted mortality rates were relatively similar among the five Brazilian regions from 2006-2008.CONCLUSIONS:
Childhood cancer mortality declined 1.2 to 1.6% per year in the South and Southeast regions. 相似文献16.
Purpose
Helicobacter pylorus (HP) is a Gram-negative spiral-shaped microaerophilic bacterium, which colonizes in the gastric mucosa of humans. The gastric human pathogen HP causes chronic gastritis and ulcers, and has a strong relationship with gastric cancer. The aim of this study was to determine advanced oxidation protein products (AOPP) levels, activities of myeloperoxidase (MPO) and catalase (CAT) in two groups.Materials and Methods
For this aim, one group included 30 patients with gastric cancer (Group 1) and the other included 30 subjects with non-gastric cancer and Anti-HP immunoglobulin (Ig) G antibody positive (group 2). Anti-HP IgG antibody test values were found as positive in fifty percent of group 1 and all of the group 2 patients.Results
Significantly increased AOOP levels were found in group 1 (p < 0.05) compared to group 2. There were no significant differences between the groups in regard to activities of MPO and CAT. In addition, AOPP level, MPO and CAT activities were similar among the Anti-HP IgG positive and negative subgroups of group 1 patients.Conclusion
The result of this study indicated that gastric cancer patients were characterized by increased protein oxidation, whereas there was no significant difference in oxidative stress parameters and antioxidant enzyme activity between the Anti-HP IgG positive and negative gastric cancer patients. 相似文献17.
Weiling Li Ye Li Yuwan Zhao Jieli Yuan Weifeng Mao 《African journal of traditional, complementary, and alternative medicines》2014,11(5):105-110
Background
To observe the inhibition effects of the Buthus matensii Karsch (BmK) scorpion venom extracts on the growth of human breast cancer MCF-7 cells, and to explore its mechanisms.Methods
Two common tumor cells (SMMC7721, MCF-7) were examined for the one which wasmore sensitivity to scorpion venom by MTT method. Cell cycle was determined by flow cytometry. Immunocytochemistry was applied to detect apoptosis-related protein Caspase-3 and Bcl-2 levels, while the expression of cell cycle-related protein Cyclin D1 was shown by Western blotting.Results
Our data indicated that MCF-7 was the more sensitive cell line to scorpion venom. The extracts of scorpion venom could inhibit the growth and proliferation of MCF-7 cells. Furthermore, the extract of scorpion venom induced apoptosis through Caspase-3 up-regulation while Bcl-2 down-regulation in MCF-7 cells. In addition, the extracts of scorpion venom blocked the cells from G0/G1 phase to S phase and decreased cell cycle-related protein Cyclin D1 level after drug intervention compared with the negative control group.Conclusions
These results showed that the BmK scorpion venom extracts could inhibit the growth of MCF-7 cells by inducing apoptosis and blocking cell cycle in G0/G1 phase. The BmK scorpion venom extracts will be very valuable for the treatment of breast cancer. 相似文献18.
Hiroaki Saito Kozo Miyatani Seigo Takaya Tomoyuki Matsunaga Yoji Fukumoto Tomohiro Osaki Toshiro Wakatsuki Masahide Ikeguchi 《Yonago acta medica》2013,56(3):73-78
Background
Although the clinicopathologic features and prognosis of Borrmann type advanced gastric cancer has been well characterized, those of advanced gastric cancer simulating early gastric cancer (AGC simulating EGC) still remains unclear.Methods
We reviewed 1985 gastric cancer patients who had undergone gastrectomy at our hospital to determine the clinicopathologic characteristics, susceptible sites for lymph node metastasis, and prognosis of AGC simulating EGC in comparison with Borrmann type advanced gastric cancer.Results
Among 102 patients with AGC simulating EGC, 100 patients (98%) had tumors with depressed type appearance. The frequencies of serosal invasion, lymph node metastasis, lymphatic vessel invasion, blood vessel invasion, and liver metastasis were significantly lower in AGC simulating EGC than in Borrmann type tumors. The prognosis of AGC simulating EGC was significantly better than that of the Borrmann type tumors. Multivariate analysis indicated that the gross appearance was an independent prognostic factor. In patients with AGC simulating EGC which invaded to the the muscularis propria (MP), most lymph node metastasis was restricted with the perigastric lymph nodes (1st-titer lymph nodes) and lymph node metastasis to 2nd-titer lymph nodes was only observed at station 8a.Conclusion
AGC simulating EGC is less advanced in comparison with Borrmann type advanced gastric cancer. Based on the results of susceptible sites for lymph node metastasis in the current study, limited lymph node dissection could be indicated for AGC simulating EGC whose depth of invasion is MP. 相似文献19.
Wang Y Liu Y Shen X Zhang X Chen X Yang C Gao H 《International journal of clinical and experimental pathology》2012,5(4):315-325
Purpose
To investigate the expression of the human PIWI subfamily proteins in gastric cancer and their potential roles in the occurrence, development and prognosis of gastric cancer.Methods and patients
Expression of the PIWI proteins were assessed by immunohistochemistry (IHC) in tissue microarrays (TMA), containing paired tumor tissue and adjacent non-cancer tissue from 182 patients who had undergone surgery in hospital for histologically proven gastric cancer (GC). Prognostic value and correlation with other clinicopathologic factors were evaluated in two classifications.Results
The expression of PIWIL1-4 was significantly higher in tumor tissue than that in adjacent tissue; A significant correlation was observed between the higher expression of PIWI protein with the T stage, lymph node metastasis and clinical TNM (cTNM); Survival analysis by Kaplan-Meier survival curve and log-rank test demonstrated that elevated PIWIL1 and PIWIL2 expression in cancer tissue predicted poorer overall survival (OS) compared with group in lower expression (36.5% VS 67.6%; 37.4% VS 54.2%; respectively). Notably, multivariate analyses by Cox’s proportional hazard model revealed that expression of PIWIL1 was an independent prognostic factor in gastric cancer.Conclusions
The PIWI subfamily protein is an absolutely key molecular factor along with the tumor occurrence and development. And the PIWI protein could act as a potential biomarker for prognosis evaluation of gastric cancer. 相似文献20.
Ana R. Gomes Ana M. Abrantes Ana F. Brito Mafalda Laranjo Jo?o E. Casalta-Lopes Ana C. Gon?alves Ana B. Sarmento-Ribeiro Maria F. Botelho José G. Tralh?o 《Clinical and molecular hepatology》2015,21(3):257-267