卵巢癌患者化疗前后血清IL-2,SIL-2R,TNF-α检测的临床意义

目的:分析了34例卵巢癌患者化疗前后血清IL-2、SIL-2R和TNF-α水平的变化。方法:采用放 射免疫分析测定患者血清中IL-2、TNF-α含量和使用ELISA法检测SIL-2R含量,并与30名正常健康人作 比较。结果:化疗前IL-2活性明显低于正常人,TNF-α和SIL-2R水平明显高于正常人(P<0.01),化疗后 6个月复发者,IL-2、TNF-α、SIL-2R水平持续异常;未复发者IL-2、TNF-α、SIL-2R水平恢复正常。结 论:观察卵巢癌患者免疫功能的变化与患者的病情及预后密切相关。     

鼻咽癌患者放疗前后血清SE-cad、IL-2、SIL-2R和TNF-α检测的临床意义

目的：探讨了鼻咽癌患者放疗前后血清SE-cad、IL-2、SIL-2R和TNF-α水平的变化。方法：应用放免法测定31例鼻咽癌患者血清IL-2、TNF-α含量，酶联法测定31例鼻咽癌患者SE-cad和SIL-2R含量，并与30名正常人作比较。结果：放疗前后血清IL-2水平明显低于正常人（P〈0．01），而SE-cad、SIL-2R和TNF-α水平明显高于正常人（P〈0．01），放疗后一年复发者血清SE-cad、IL-2、SIL-2R和TNF-α水平持续异常，未复发者血清SE-cad、IL-2、SIL-2R和TNF-α水平恢复正常。结论：观察鼻咽癌患者血清SE-cad、IL-2、SIL-2R和TNF-α水平的变化与患者的病情及预后密切相关。     

2型糖尿病肾病患者血清 IL-2、SIL-2R 和 TNF-α 检测的临床意义

目的:探讨2型糖尿病肾病患者血清IL-2、SIL-2R和TNF-α水平变化在肾病发生、发展过程中的作用.方法:对42例2型糖尿病肾病患者采用放免法检测血清IL-2、SIL-2R和ELISA法检测TNF-α水平,并与35名正常人作比较.结果:2型糖尿病肾病患者血清SIL-2R和TNF-q水平显著高于正常人(P＜0.01),而IL-2水平则显著低于正常人(P＜0.01).结论:血清IL-2、SIL-2R和TNF-α水平的变化在2型糖尿病肾病的发生和发展中相互作用,观察其浓度的变化对探讨2型糖尿病肾病的发病机理、预防和指导用药均有十分重要的临床价值.     

EMS患者治疗前后血清SIL-2R、TNF-α和CA199检测的临床意义

目的:探讨子宫内膜异位症患者治疗前后血清SIL-2R、TNF-α和CA199水平的变化及意义.方法:应用放免法测定40例子宫内膜异位症患者血清TNF-α和CA199含量、酶联法测定SIL-2R含量,并与35名正常健康人作比较.结果:在治疗前,血清SIL-2R、TNF-α和CA199水平非常显著地高于正常人组(P＜0.01),经治疗1个月后,与正常人组比较仍有显著差异(P＜0.05).结论:检测子宫内膜异位症患者血清SIL-2R、TNF-α和CA199水平的变化对其病情和预后判断均有重要的临床意义.     

子宫内膜异位症患者治疗前后血清IL-2、SIL-2R和TNF-α检测的临床意义

目的:探讨子宫内膜异位症患者治疗前后血清IL-2、SIL-2R和TNF-α水平的变化意义。方法:应用放射免疫分析和酶联免疫吸附法测定了42例子宫内膜异位症患者血清IL-2、SIL-2R和TNF-α含量,并与35名正常健康人作比较。结果:在治疗前血清SIL-2R和TNF-α水平非常显著地高于对照组(P<0.01),而IL-2水平则显著地低于对照组(P<0.01),经治疗3个月后,则与对照组比较无显著性差异(P>0.05)。结论:子宫内膜异位症的发生与发展与血清IL-2、SIL-2R和TNF-α水平密切相关。     

白癜风患者治疗前后血清IL-2、SIL-2R、TNF-α和外周血B细胞及T淋巴细胞亚群检测的临床意义

目的:探讨白癜风患者治疗前后血清IL-2、SIL-2R、TNF-α和外周血B细胞T淋巴细胞亚群水平及临床意义.方法:分别应用放免法、ELISA法和单克隆抗体法检测40例白癜风患者治疗前后血清IL-2、SIL-2R、TNF-α和外周血B细胞、T淋巴细胞亚群水平,并与35名正常健康人作比较.结果:白癜风在治疗前血清SIL-2R、TNF-α和外周血B细胞水平均非常显著地高于正常人组(P＜0.01),而IL-2、CD3、CD4、CD4/CD8比值则显著的低于正常人组(P＜0.01),经治疗后6个月,与正常人比较仍有显著性差异(P＜0.05).结论:白癜风患者存在自身免疫调节异常.     

血小板减少性紫癜患儿治疗前后血清IL-2、SIL-2R检测的临床意义

目的:分析了31例血小板减少性紫癜患儿治疗前后血清IL-2、SIL-2R水平的变化。方法:采用放射免疫分析检测患者血中IL-2含量和ELISA法检测SIL-2R含量,并与35名正常健康儿童作比较。结果:治疗前血清IL-2水平明显低于正常人,SIL-2R水平明显高于正常人(P<0.01),经1个月治疗后,血清IL-2和SIL-2R与正常人比较无显著差异(P>0.05)。结论:观察血小板减少性紫癜患者的免疫功能变化与患者的病情和预后密切相关。     

慢性肾病患者血清SIL-2R、TNF-α和血浆VEGF检测的临床意义

目的:探讨慢性肾病患者血清SIL-2R、TNF-α和血浆VEGF水平的变化及意义.方法:应用放免法和酶联双抗体法对32例慢性肾病患者进行了血清SIL-2R、TNF-α和血浆VEGF水平测定,并与35名正常健康人作比较.结果:慢性肾病患者血清SIL-2R、TNF-α和血浆VEGF水平显著地高于正常人组(P＜0.01),经治疗6个月后与正常人比较仍有差异(P＜0.05).结论:慢性肾病的发生、发展与SIL-2R、TNF-α和VEGF水平密切相关.     

肺癌患者化疗前后SOD、SIL-2R和TNF-α检测的临床意义

目的：探讨了肺癌患者化疗前后血清SOD、SIL-2R和TNF-α含量的变化及意义。方法：应用放射免疫分析和酶联免疫法测定了39例肺癌患者血清中SOD、SIL-2R和TNF-α含量,并与35名正常健康人作比较。结果：肺癌患者在化疗前血清中SIL-2R和TNF-α水平非常显著地高于正常人组（P〈0.01）,而SOD则显著低于正常人组（P〈0.01）。化疗后6个月与正常人比较仍有显著性差异（P〈0.05）。结论：肺癌患者的发生与发展与血清中SOD、SIL-2R和TNF-α水平密切相关。     

卵巢癌患者化疗前后血清CA125、IL-2和SIL-2R检测的临床应用

目的:探讨了卵巢癌患者化疗前后血清CA125、IL-2和SIL-2R水平的变化及临床意义。方法:采用放射免疫分析和酶联法对36例卵巢癌患者进行了化疗前后血清CA125、IL-2和SIL-2R检测,并与35名正常健康妇女作比较。结果:卵巢癌患者在化疗前血清CA125、SIL-2R水平非常显著地高于正常妇女组(P<0.01),而IL-2水平又非常显著地低于正常妇女组(P<0.01),化疗后6个月复发者血清IL-2、CA125、SIL-2R水平持续异常,未复发者血清IL-2、CA125、SIL-2R水平恢复正常。结论:观察卵巢癌患者免疫功能的变化与患者的病情及预后密切相关。     

CaO-MgO-P_2O_5-SiO_2-CaF_2系统自硬性玻璃的研究

为研究钙离子、镁离子在体内环境中对自硬性玻璃结晶行为的影响,为自硬性生物活性玻璃的临床应用提供依据,本文设计了CaO-P2O5-SiO2-CaF2(Ca-glass)和CaO-MgO-P2O5-SiO2-CaF2(CaMg-glass)系统玻璃并使用模拟体液(simulated body flu id,SBF)进行了研究。首先采用磷酸氢二氨[(NH4)2HPO4]/[NH4H2PO4]硬化液与Ca-glass、CaMg-glass制成硬化体,然后使用X射线衍射(XRD)、扫描电镜(SEM)、失重、力学分析等方法,研究硬化体在SBF中的结晶性、降解性和力学性能。实验结果表明,玻璃粉末与磷酸铵缓冲溶液反应形成了磷酸铵钙[(NH4)2.Ca(HPO4)2.H2O]硬化体。硬化体经过SBF浸泡,Ca-glass系统硬化体中部分磷酸铵钙转化成羟基磷灰石,而CaMg-glass系统硬化体仍然为磷酸铵钙。Ca-glass与CaMg-glass硬化体在SBF中浸泡28天分别降解19.4%和31.3%,抗压强度分别为93.14MPa和64.52MPa。镁离子的歧化作用是导致Ca-glass、CaMg-glass硬化体结晶性能、降解性能以及力学性能差别的主要原因。     

Dichotomization: 2 × 2 (×2 × 2 × 2...) categories: infinite possibilities

Background  

Consumers of epidemiology may prefer to have one measure of risk arising from analysis of a 2-by-2 table. However, reporting a single measure of association, such as one odds ratio (OR) and 95% confidence interval, from a continuous exposure variable that was dichotomized withholds much potentially useful information. Results of this type of analysis are often reported for one such dichotomization, as if no other cutoffs were investigated or even possible.     

2-Perbromomethyl-2-oxazoline: a novel trifunctional initiator for the ring-opening polymerization of 2-methyl-2-oxazoline

Polymerization of 2-methyl-2-oxazoline was carried out using a trifunctional initiator, 2-perbromomethyl-2-oxazoline. The degree of polymerization (DP) of the resulting polymer was very close to the feed mole ratio of the monomer to initiator. The number-average molecular weight M?_n increased linearly with conversion, indicating the living nature of the propagating chain end. ¹H NMR and end-group analyses results are consistent with the proposal that the polymer possesses a star-shaped structure.     

Corticosteroid-interleukin 2 interactions: inhibition of binding of interleukin 2 to interleukin 2 receptors.

PHA activated human peripheral blood mononuclear cells (MNC) were incubated with human interleukin 2 (IL-2) in the absence or in the presence of 10(-6)-10(-9) M hydrocortisone (HC). HC suppressed the proliferative response of the activated MNC to highly purified human leucocyte IL-2 in a dose dependent manner. This suppression was in full accordance with an inhibition of the IL-2 binding capacity, whereby the high affinity binding sites were reduced by 85%, while the low affinity sites were less affected. The mechanism by which HC inhibits the binding of IL-2 is still unknown. Evidence is presented that HC binds only weakly to IL-2. We conclude that HC interferes with the IL-2 binding by modulating the binding and/or signal processing function of the IL-2 receptor.     

Isolation of a novel KIR2DL3-specific mAb: comparative analysis of the surface distribution and function of KIR2DL2, KIR2DL3 and KIR2DS2

In recent years an increasing number of sequences coding for new KIRs have been described. However, the limited availability of mAbs with unique KIR specificities has hindered an exhaustive assessment of their actual function, HLA-specificity, expression at the cell surface and distribution in different cell populations. In this study we report the generation of a novel mAb (ECM41) specific for KIR2DL3 molecules. By the use of cell transfectants expressing one or other KIR we show that this reagent allows discrimination of KIR2DL3 from other GL183 mAb-reactive molecules such as KIR2DL2 and KIR2DS2. Moreover we show that this novel mAb can be used to assess the surface expression and distribution of KIR2DL3 in different polyclonal NK populations and in NK cell clones. Along this line, we were able to analyze the HLA class I specificity of NK clones expressing either KIR2DL3 or KIR2DL2, two inhibitory receptors that were so far serologically undistinguishable. Finally, the combined use of GL183 and ECM41 mAbs in redirected killing assays allowed us to investigate the functional outcome of the simultaneous engagement of KIR2DL3 and KIR2DS2 in NK cell clones co-expressing KIRs that display opposite (inhibitory vs activating) function.     

13C NMR spectroscopy of 2-formamido-2-methylpropyl acrylate and poly(2-formamido-2-methylpropyl acrylate)

The synthesis and characterization of 2-formamido-2-methylpropyl acrylate (FMPA) is reported. ¹³C NMR spectra of FMPA in CDCl₃, CD₃OD, DMSO-d₆, DMF-d₇, and D₂O exhibit two pairs of lines for all seven carbon atoms at room temperature; the ratio of the two conformers varies moderately with solvent (21 : 79 to 41 : 59). The conformers are believed to involve strong internal hydrogen bonding which is not completely broken even by the addition of trifluoroacetic acid to CDCl₃ (1/1, v/v). However, the pairs of lines coalesce in turn as the temperature is raised to 120°C in DMSO-d₆. FMPA was polymerized at 35°C in DMF and CHCl₃, using a free radical initiator and the polymer was characterized by ¹³C NMR spectroscopy.     

肌肽减轻H_2O_2诱导的N2a细胞损伤

目的探究肌肽对H2O2诱导的N2a细胞损伤的保护作用及其机制。方法将N2a细胞分为对照组、损伤组(给予H2O2)和肌肽保护组(预先加入肌肽)。用相差显微镜观察了细胞形态,用免疫荧光染色和免疫印迹实验检测细胞AKT1和FAS-L的蛋白表达。结果肌肽显著增加了N2a细胞增殖能力(P0.05);与对照组比较,H2O2损伤组细胞形态发生明显改变,细胞皱缩,胞膜变厚并出现发泡现象;而肌肽保护组细胞形态较损伤组明显改善;免疫荧光染色和免疫印迹实验显示,损伤组AKT1的表达显著低于对照组,FAS-L的表达显著高于对照组。而肌肽保护组AKT1的表达显著高于对照组,FAS-L的表达显著低于对照组(P0.05)。结论肌肽能够减轻N2a细胞对H2O2诱导的细胞损伤。     

An apparent KIR2DS2-negative KIR2DL2-positive genotype discloses the novel allele KIR2DS2*00104

KIR2DS2*00104 lacks a distinctive synonymous substitution of KIR2DS2 in nucleotide 418 that affects KIR genotyping.     

Mycoplasmacidal Activity of Peroxidase-H2O2-Halide Systems

A mycoplasmacidal system consisting of myeloperoxidase (MPO)-containing granules, H(2)O(2), and a halide is described. In all parameters measured, it appears to be identical to the MPO-H(2)O(2)-halide bactericidal system previously reported. It has a pH optimum of approximately 5.5 and an optimal MPO:H(2)O(2) ratio of 1:25. The halide requirement can be satisfied by either chloride or iodide. Through the use of taurine or horseradish peroxidase substitution, chloride-mediated killing can be distinguished from iodide-mediated killing. The relationship of this mycoplasmacidal system to other mycoplasmacidal systems and to host surveillance of mycoplasma is discussed.     

NDRG2对肝癌细胞HepG2凋亡的诱导作用

目的：探讨NDRG2对肝癌细胞HepG2凋亡的诱导作用。方法：用RT-PCR获取NDRG2基因。测序判断正确后，将其克隆入含绿色荧光蛋白(GFP)基因的真核表达载体pIRES2-EGFP中，并转染NDRG2表达阴性的HepG2细胞。用光镜观察转染细胞的形态变化；荧光显微镜观察NDRG2蛋白的定位；流式细胞仪分析细胞周期的改变；透射电镜进一步观察细胞超微结构的改变。结果：获得了NDRG2基因，成功地构建了pIRES2-EGFP-NDRG2真核表达载体。转染HepG2细胞后，细胞生长受抑，结构破坏并死亡。荧光显微镜观察到NDRG2在胞质中表达，流式细胞仪检测出现G1期阻滞和凋亡峰，透射电镜观察到典型的细胞凋亡特征。结论：NDRG2基因具有诱导HepG2细胞凋亡的作用，其机制有待进一步研究。