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1.
脉冲电刺激对血管内皮细胞与其祖细胞黏附的影响   总被引:1,自引:0,他引:1  
Li W  Zheng L  Wang Q  Guo S 《生物医学工程学杂志》2011,28(4):689-93, 697
为探索脉冲电刺激下血管内皮细胞与内皮祖细胞(EPC)之间黏附强度的改变,诱导培养外周血EPC,荧光标记后与单层血管内皮细胞共培养,固定电压和频率为5 V和5 Hz,选择1、3、6、9 ms的脉宽对其进行干预,持续刺激24 h后检测贴壁EPC的荧光强度,以荧光比率衡量。结果显示,与对照组相比,3 ms刺激组荧光比率即显著增高,随着脉宽延长,6 ms组达到最大值,但9 ms刺激组却显著下降,提示适宜脉冲电刺激有利于血管内皮细胞与EPC之间的黏附,为电刺激促进血管新生的研究提供新的理论依据。  相似文献   

2.
目的观察脉冲电刺激对H9c2细胞增殖的抑制作用,探讨脉冲电刺激对H9c2细胞分化的诱导作用。方法用不同频率(0、1、5、10、50、100 Hz)、电压(0、5、10、20、40、50 V)、时间(0、1、2、4、6、8 h/d)的组合脉冲电刺激作用于大鼠H9c2细胞,以5-氮胞苷作为干预因素对照。采用MTT法检测H9c2细胞增殖率。采用免疫荧光细胞化学染色检测心肌肌钙蛋白T(cTNT)及八聚体结合转录因子4(Oct4)的表达。采用RT-PCR法检测cTNT、肌球蛋白重链α(α-MHC)mRNA的表达。结果脉冲电刺激能抑制H9c2细胞增殖(P<0.05),脉冲电刺激的参数为频率1 Hz、4 h/d、10 V时对H9c2细胞增殖的抑制作用最大且不致细胞死亡。用此参数脉冲电刺激作用于H9c2细胞2周后细胞Oct4表达减少(P<0.05),而cTNT和α-MHC表达仍十分明显。结论脉冲电刺激能抑制大鼠胚胎心脏组织成肌细胞株H9c2细胞的增殖,同时cTNT和α-MHC表达增强,Oct4表达减弱,对H9c2细胞具有诱导分化作用。  相似文献   

3.
目的研究不同的电刺激信号对大鼠雪旺细胞(RSC96)增殖的影响。方法实验使用RSC96细胞系(中国科学院典型培养物保藏委员会细胞库),经过2次传代培养,以1.25×10~4/孔的密度种植于24孔细胞培养板中,次日电刺激信号分别采用不同的电压(10、100、500、1 000、5 000、10 000 m V/cm)、不同的频率(0.1、1.0、100.0、1 000.0、10 000.0、1 000 000.0 Hz)、不同的脉冲占空比(5%、10%、30%、50%、70%、90%)及不同的波形(正脉冲波、矩形波、三角波、正弦波、杂波、直流波)对细胞进行电刺激,每组设置3个平行样,细胞每天刺激30 min,持续刺激4 d。采用四甲基偶氮唑盐(MTT)法检测细胞增殖率。结果当电刺激频率为100.0 Hz、脉冲占空比为50%、波形为矩形波时,电压500 m V/cm时对细胞的增殖具有抑制作用。当电刺激频率为100.0 Hz、脉冲占空比为50%、电压为500 m V/cm时,正脉冲波、正弦波、矩形波和三角波都能够促进细胞的增殖。当电刺激波形为矩形波、脉冲占空比为50%、电压为500 m V/cm时,频率100.0~1 000.0 Hz组细胞增殖率最高。当电刺激波形为矩形波、频率为100.0 Hz、电压为500 m V/cm时,不同的脉冲占空比对细胞增殖的影响不明显。结论不同的电刺激信号条件对细胞增殖的影响较大。电压≤500 m V/cm、有规律的脉冲波形、频率为100.0~1 000.0 Hz电刺激信号能够获得较好的RSC96增殖的效果。实验所获得的优化电刺激参数在外周神经修复中具有非常大的应用前景。  相似文献   

4.
重组人促红细胞生成素对体外培养内皮细胞增殖的影响   总被引:1,自引:0,他引:1  
目的:研究重组人促红细胞生成素(rhEPO)对体外培养内皮细胞增殖的影响.方法:从脐静脉体外分离培养人脐静脉内皮细胞(HUVEC),采用形态学观察及Ⅷ因子相关抗原(Ⅷ-R Ag)检测进行内皮细胞鉴定,免疫组织化学方法检测rhEPO对HUVEC增殖细胞核抗原(PCNA)与细胞周期蛋白D1(Cyclin D1)的表达变化.结果:rhEPO实验组内皮细胞PCNA与Cyclin D1表达均明显高于对照组.当rhEPO剂量提高到20U/ml时其作用最强.结论:rhEPO对内皮细胞增殖有明显促进作用,这种促进作用町能是其影响血管生成的主要原因之一.  相似文献   

5.
研究了功能电刺激作用下的人体神经肌肉的疲劳特性和生物反应,初步确定了适合于人体神经肌肉电刺激脉冲信号的基本特征,以正常男性成人的曲肘运动(右手)为研究对象,将表面电极置于右手肱二头肌肌腹处,在肘关系(右手)处安装一个微型角位移传感器,通过多功能神经肌肉电刺激肢体运动测控仪器,获取曲肘运动时的肘关节角位移变化曲线,结果表明,在任何一种刺激模式作用下,曲肘运动均呈现严重非线性时变特性;缓慢连续变化的刺激脉冲能有效地缓解肱二头肌的疲劳程度,最佳的功能电刺激脉冲频率为30Hz-50Hz。  相似文献   

6.
目的:建立体外背根节神经元与Schwann细胞联合培养模型,观察短时低频电刺激对Schwann细胞髓鞘蛋白表达的影响。方法:培养和纯化背根节神经元与Schwann细胞,制成背根节神经元/Schwann细胞联合培养体系。于L-ascorbic acid诱导的同时,施予低频电刺激(20Hz,100μs,3V),持续作用1h,分别于L-ascorbicacid诱导后第0,2,4,8和10d取各组培养基上清以ELISA测定其中脑衍生物神经生长因子(BDNF)的水平。另外,于诱导后第7d和14d检测培养体系中髓鞘蛋白P0的表达。结果:电刺激组各时间点BDNF的浓度较对照组显著升高(P0.01)。经电刺激作用后,联合培养体系中P0表达上调(P0.05)。然而,电刺激结束后在培养液中加入TrkB-Fc,P0的表达水平则显著降低(P0.05)。结论:在神经元存在的条件下,短时低频电刺激可促进离体Schwann细胞合成P0,初步认为该作用通过刺激神经元分泌BDNF增多所致。  相似文献   

7.
目的研究硫化氢(H2S)在雌激素促进血管内皮细胞增殖中的作用。方法利用培养的脐静脉血管内皮细胞,以雌激素处理后,Western blot检测H2S产生关键酶CSE(胱硫醚-γ-裂解酶)的蛋白表达情况;亚甲基蓝法测定血管内皮细胞释放H2S含量;MTT法检测血管内皮细胞增殖情况。结果不同浓度(1nmo1/L~1μmol/L)的雌激素处理血管内皮细胞48h后.均可促进CSE蛋白表达和内皮H2S释放。不同浓度的雌激素均能明显促进血管内皮细胞增殖。以CSE特异性抑制剂PPG抑制H2S产生后,雌激素促进血管内皮增殖的能力受到显著抑制。结论雌激素可通过促进CSE蛋白表达促进血管内皮细胞释放H2S,进而促进血管内皮细胞增殖。  相似文献   

8.
目的:探讨电刺激对脑梗死大鼠运动功能及骨形成蛋白2、6、7(BMP2,6,7)表达的影响。方法:健康雄性SD大鼠,制作大脑中动脉闭塞模型,将造模成功的大鼠随机分为对照组、电刺激组各48只,假手术组32只,正常组8只。对照组、电刺激组、于脑梗死后每天接受自然恢复、电刺激治疗6 h、12 h、24 h、3 d、7 d和21 d。并在上述时间点,各组取8只大鼠,进行走平衡木试验观察大鼠偏瘫肢体功能恢复情况,然后断头处死动物取脑,石蜡包埋切片行免疫组织化学染色,检测梗死灶周围皮质及正常大脑皮质BMP2,6,7的表达。结果:第7天、21天时,电刺激组肢体功能恢复较对照组明显(P0.05)。正常组和假手术组BMP-2,6,7表达无统计学差异。对照组、电刺激组BMP-2表达均先下调,电刺激组大鼠BMP-2下调时间早于对照组,21 d恢复至正常水平,对照组、电刺激组BMP-6,7均上调,BMP-7于7 d灰度值上升,至21 d恢复至正常水平,BMP6则一直保持高水平,电刺激组BMP-6,7表达高于对照组。结论:电刺激组偏瘫肢体功能的恢复优于对照组,电刺激下调BMP-2表达,上调BMP-6,7表达。  相似文献   

9.
目的:观察HGF基因对人脐静脉内皮细胞增殖、迁移的影响。方法:从已有质粒pRc/CMV-HGF中扩增出HGF基因,将其克隆到含增强型绿色荧光蛋白的真核表达载体中,构建重组质粒pEGFP-HGF,酶切及测序鉴定正确后,用脂质体将重组质粒pEGFP-HGF转染到人脐静脉细胞株ECV304中,G418筛选获得稳定表达细胞克隆,采用荧光显微镜观察、RT-PCR、免疫细胞化学方法检测鉴定重组质粒的表达情况;酶联免疫吸附法(ELISA)检测稳定表达细胞中HGF的含量;再以MTT法检测转染重组质粒后细胞增殖的改变,Transwell Migration实验检测细胞迁移能力的改变。结果:所构建重组质粒经酶切图谱分析和序列测定证实构建成功;荧光显微镜下观察到有绿色荧光;RT-PCR证实HGFmRNA在转染阳性细胞高表达;免疫细胞化学证实转染pEGFP-HGF质粒的细胞有HGF蛋白的表达;ELISA检测细胞培养基中HGF含量可达112.3 ng/ml,MTT法、TranswellMigration测定转染pEGFP-HGF阳性细胞的增殖、迁移能力明显高于对照组(P<0.01)。结论:重组质粒pEGFP-HGF能够在内皮细胞株ECV304转录、表达;表达的活性蛋白HGF刺激ECV304的增殖、迁移,为进一步应用于基因治疗奠定实验基础。  相似文献   

10.
氧化修饰低密度脂蛋白对内皮细胞功能的影响   总被引:6,自引:0,他引:6  
目的 观察氧化修饰低密度脂蛋白(ox-LDL)对内皮细胞NO/ET-1、t-PA/PAI-1合成、细胞表面细胞间黏附分子-1(ICAM-1)表达的影响。方法 采用培养的人脐静脉内皮细胞(HUVECs),在分另0加入不同浓度ox-LDL(50、100、150、200mg/L)孵育24h后,观察培养液中NO、ET-1、t-PA、PAI-1水平及细胞表面ICAM-1的表达。结果 ox-LDL可显著抑制NO、t-PA的合成。ox-LDL还可明显增加ET-1、PAI-1的分泌及诱导细胞表面ICAM-1的表达。结论 ox-LDL对内皮细胞具有明显的细胞毒作用,它对NO/ET-1、t-PA/PAI-.1及ICAM-1表达的影响可能是其致动脉粥样硬化发生的重要机制之一。  相似文献   

11.
Summary The power spectral analysis of R-R interval variability (RRV) has been estimated by means of an autoregressive method in seven sedentary males at rest, during steady-state cycle exercise at 21 percent maximal oxygen uptake. (% V O 2max), SEM 2%, 49% VO 2max, SEM 2% and 70% VO 2max, SEM 2% and during recovery. The RRV, i.e. the absolute power of the spectrum, decreased 10, 100 and 500 times in the three exercise intensities, returning to resting value during recovery. In the RRV power spectrum three components have been identified: (1) high frequency peak (HF), central frequency about 0.24 Hz at rest and recovery, and 0.28 Hz, SEM 0.02, 0.37 Hz, SEM 0.03 and 0.48 Hz, SEM 0.06 during the three exercise intensities, respectively; (2) low frequency peak (LF), central frequency about 0.1 Hz independent of the metabolic state; (3) very low frequency component (VLF), <0.05 Hz, no peak observed. The HF peak power, as a percentage of the total power (HF%), averaged 16%, SEM 5% at rest and did not change during exercise, whereas during recovery it decreased to 5%–10%. The LF% and VLF% were about 50% and 35% at rest and during low exercise intensity, respectively. At higher intensities, LF% decreased to 16% and VLF% increased to 70%. During recovery a return to resting values occurred. The HF component may reflect the increased respiratory rate and the LF peak changes the resetting of the baroreceptor reflex with exercise. The hypothesis is made that VLF fluctuations in heart rate might be partially mediated by the sympathetic system.  相似文献   

12.
Cerebral aneurysms and arteriovenous malformations (AVM) are a common cause of stroke and cerebral hemorrage. Both are often discovered when they rupture, causing subarachnoid hemorrhage (SAH). SAH-induced vasospasm is mediated by enhanced vasoconstriction due to endothelin-1 (ET-1). We investigated whether endothelial cells (ECs) obtained from aneurysm and AVM express phenotypic and genotypic alterations contributing to the development of vasospasm after SAH. We isolated ECs from human AVM and aneurysm and then confirmed their EC origin by polymerase chain reaction and immunocytochemistry with endothelial markers. Experiments were also carried out with human cerebral microvascular and umbilical vein ECs (HCECs and HUVECs respectively) for comparison. We tested EC proliferation ability and microtubule formation in Matrigel at different cell passages. Five aneurysm (3 ruptured, 2 unruptured) and 3 AVM (2 ruptured, 1 unruptured) ECs were tested for ET-1 release in the culture medium. Aneurysm and AVM ECs expressed von Willebrand factor, Adrenomedullin, and exhibited a progressive reduction of proliferation and in vitro angiogenic ability after the V passage. Significantly higher levels of ET-1 have been detected in ECs from ruptured aneurysms and AVMs. We report the first successful isolation and characterization of primary EC lines from human cerebral vascular lesions. Augmented release of ET-1 is correlated with the rupture of the abnormal vessel confirming its role in vasospasm after SAH. Furthermore, ECs obtained from these vascular malformations can be used as an experimental model to study SAH-induced vasoconstriction.  相似文献   

13.
目的 探讨阿司匹林(Aspirin)对内皮素-1(ET-1)诱导的大鼠心脏成纤维细胞(CFs)增殖的干预作用及可能机制。方法 经差速贴壁法培养的新生大鼠CFs,随机分为7组:对照组、ET-1组、阿司匹林组、ET-1+ 阿司匹林1、2、5和10 mmol·L-1组。用四氮唑盐(MTT)法测定CFs数目,流式细胞仪检测CFs细胞周期,液体闪烁计数仪测定CFs 3H-脯氨酸掺入率,硝酸还原酶法测定细胞培养上清液中NO含量。结果 与对照组相比,10-7 mol·L-1 ET-1能显著促CFs增值及[3H]-Proline掺入率,降低CFs生成NO2-/NO3-的量(均P < 0.01),1~10 mmol·L-1 阿司匹林呈浓度依赖性的缓解上述变化(均P < 0.05); ET-1能显著提高S期细胞百分率(P < 0.01),10 mmol·L-1 阿司匹林抑制ET-1诱导S期细胞百分率上升(P < 0.01)。结论 阿司匹林抑制ET-1诱导的CFs增殖及胶原合成可能和NO生成有关。  相似文献   

14.
次声作用后血浆NO、ET-1、SOD、MDA水平的变化   总被引:3,自引:2,他引:1       下载免费PDF全文
目的:测定8 Hz、130 dB次声不同时间暴露后大鼠血浆一氧化氮(NO)、内皮素(ET-1)、SOD、MDA水平的变化。方法:用8 Hz、130 dB的次声连续作用大鼠1、7、14、21和28 d,每天2 h,测定大鼠血浆NO、ET-1、SOD、MDA水平。结果:在暴露期间,7、14 d时大鼠血浆NO含量显著最低(P<0.01),1 d、21 d和28 d时正常(P>0.05);大鼠血浆ET-1含量均明显升高(P<0.01),7 d时升高最多,14 d时升高最少;大鼠血浆SOD活性明显降低(P<0.01);大鼠血浆MDA水平明显升高(P<0.01)。结论:次声可引起大鼠血浆NO、ET-1、SOD、MDA水平的变化,发生的改变与次声暴露时间有关。  相似文献   

15.
Presently the majority of tissue engineering approaches aimed at regenerating bone relies only on post-implantation vascularization. Strategies that include seeding endothelial cells (ECs) on biomaterials and promoting their adhesion, migration and functionality might be a solution for the formation of vascularized bone. Nano/micro-fiber-combined scaffolds have an innovative structure, inspired by extracellular matrix (ECM) that combines a nano-network, aimed to promote cell adhesion, with a micro-fiber mesh that provides the mechanical support. In this work we addressed the influence of this nano-network on growth pattern, morphology, inflammatory expression profile, expression of structural proteins, homotypic interactions and angiogenic potential of human EC cultured on a scaffold made of a blend of starch and poly(caprolactone). The nano-network allowed cells to span between individual micro-fibers and influenced cell morphology. Furthermore, on nano-fibers as well as on micro-fibers ECs maintained the physiological expression pattern of the structural protein vimentin and PECAM-1 between adjacent cells. In addition, ECs growing on the nano/micro-fiber-combined scaffold were sensitive to pro-inflammatory stimulus. Under pro-angiogenic conditions in vitro, the ECM-like nano-network provided the structural and organizational stability for ECs' migration and organization into capillary-like structures. The architecture of nano/micro-fiber-combined scaffolds elicited and guided the 3D distribution of ECs without compromising the structural requirements for bone regeneration.  相似文献   

16.
The purpose of this study is to verify the features of the power spectrum of postural tremors for neuromuscular disease patients and to classify the postural tremors. The subjects were 88 neuromuscular disease patients (30 Parkinson disease (PD), 25 cerebellar disease (CER), 7 multiple sclerosis (MS), 7 neuropathy (NEU), 10 motor neuron disease (MND), 9 myopathy (MYO)). The control subjects were 12 normal young persons and 10 normal aged persons. Postural tremor was detected by accelerator sensor. Postural tremor was recorded under the two postural conditions: The subjects maintained the index finger without or with a weight load of 50 g in a horizontal position while looking at a visual target in front of the tip of the index finger. The power spectrum was calculated by an auto-regressive model (AR model). The peak frequency and the peak power were evaluated under the two conditions. Two frequency components of 8-12 Hz and 20-25 Hz appeared in the postural tremor of both normal subjects and neuromuscular disease patients. The difference of the postural tremor between the subjects mainly appeared in the 8-12 Hz component during the postural tremor with a weight load. MYO patients belonged to one group (called as group P1) due to lower peak power, CER patients belonged to one group (called as group P2) due to higher peak power, and PD and MS patients belonged to one group (called as group P3) due to lower peak frequency and higher peak power. NER and MND patients belonged to one group (called as group N which meant normal group). These results suggested that the peak frequency and the peak power of the 8-12 Hz component were changed by the conditions of both spinal reflex system and central nervous system. An oscillator within the central nervous system produced the underlying frequency of 8-12 Hz component, while the amplitude of 8-12 Hz component was governed by both spinal reflex system and central nervous system. In conclusion, the classification of postural tremor for neuromuscular disease patients was a useful index to elucidate the mechanism of tremor oscillation and to assist in clinical diagnosis of neuromuscular disease.  相似文献   

17.
Hentall ID  Pinzon A  Noga BR 《Neuroscience》2006,142(3):893-903
The monoamine neurotransmitter serotonin is released from spinal terminals of nucleus raphe magnus (NRM) neurons and important in sensory and motor control, but its pattern of release has remained unclear. Serotonin was measured by the high-resolution method of fast cyclic voltammetry (2 Hz) with carbon-fiber microelectrodes in lumbar segments (L3-L6) of halothane-anesthetized rats during electrical stimulation of the NRM. Because sites of serotonin release are often histologically remote from membrane transporters and receptors, rapid emergence into aggregate extracellular space was expected. Increased monoamine oxidation currents were found in 94% of trials of 50-Hz, 20-s NRM stimulation across all laminae. The estimated peak serotonin concentration averaged 37.8 nM (maximum 287 nM), and was greater in dorsal and ventral laminae (I-III and VIII-IX) than in intermediate laminae (IV-VI). When measured near NRM-evoked changes, basal monoamine levels (relative to dorsal white matter) were highest in intermediate laminae, while changes in norepinephrine level produced by locus ceruleus (LC) stimulation were lowest in laminae II/III and VII. The NRM-evoked monoamine peak was linearly proportional to stimulus frequency (10-100 Hz). The peak often occurred before the stimulus ended (mean 15.6 s at 50 Hz, range 4-35 s) regardless of frequency, suggesting that release per impulse was constant during the rise but fell later. The latency from stimulus onset to electrochemical signal detection (mean 4.2 s, range 1-23 s) was inversely correlated with peak amplitude and directly correlated with time-to-peak. Quantitative modeling suggested that shorter latencies mostly reflected the time below detection threshold (5-10 nM), so that extrasynaptic serotonin was significantly elevated well within 1 s. Longer latencies (>5 s), which were confined to intermediate laminae, appeared mainly to be due to diffusion from distant sources. In conclusion, except possibly in intermediate laminae, serotonergic volume transmission is a significant mode of spinal control by the NRM.  相似文献   

18.
目的 :探讨髂股动脉硬化闭塞症 (IFAO)人工血管转流术后血浆内皮素 - 1(ET - 1)、一氧化氮 (NO)水平的变化规律及其临床意义。方法 :选择 2 0例行主 -股动脉转流术的IFAO患者 ,分别于手术前、手术后 1、3、7、14天以及手术后 1个月、6个月和 12个月抽取空腹静脉血 5ml,制备血浆标本 ,分别利用放射免疫分析和Grisse方法测定血浆ET - 1、NO水平 ,同时观察肢体缺血性表现和相关影像学检查指标的变化 ,分析ET - 1、NO水平与临床治疗效果和并发症的关系。结果 :16例手术效果良好者 ,血浆ET - 1水平于术后 1天明显升高 ,术后 3天开始下降 ,于术后 14天恢复正常水平 ,而NO则呈相反变化曲线 ,亦于术后 14天恢复正常 ;4例术后血运得到改善者 ,术后 3个月后缺血症状加重 ,并逐渐发现吻合口高度狭窄或闭塞 ,其血浆ET - 1和NO水平术后 2周内得到改善 ,但后期再次出现高ET - 1血症和低NO血症。结论 :IFAO患者及术后再狭窄患者存在高ET - 1血症和低NO血症 ,其高ET - 1和低NO现象于术后 2周内随肢体血运的改善而纠正 ,但当吻合口狭窄时再次出现高ET - 1血症和低NO血症 ;围手术期动态观察血浆ET - 1和NO水平 ,有助于评价治疗效果、判断预后和预测吻合口再狭窄的发生。  相似文献   

19.
ET-1促进人血管平滑肌细胞的表型变化和增殖   总被引:12,自引:1,他引:11  
目的:研究ET-1与VSMC表型变化和增殖的关系。方法:用MTT法研究ET—1对平滑肌细胞增殖的影响,^3H-TdR法测定ET-1对平滑肌细胞DNA合成的作用,流式细胞仪法观察对平滑肌细胞增殖周期的影响,逆转录聚合酶链法观察对平滑肌细胞表型变化的影响。结果:与对照组比较,ET-1明显促进平滑肌细胞增殖;促进平滑肌细胞DNA合成;促进平滑肌细胞从G0期向S期的转变,G0/G1期细胞百分比明显降低,而S期细胞百分比增加;促进平滑肌细胞的表型转化,从第2d到第7d随时间延长1-Caldesmon表达逐渐增高。结论:ET-1促进平滑肌细胞表型转化,同时对平滑肌细胞增殖亦有明显的促进作用。  相似文献   

20.
To determine the relationship between the urinary endothelin (ET-1), nitric oxide (NO) levels and the clinical, pathologic types of primary glomerulonephritis (GN) patients, urinary levels of ET-1 and NO were detected in 27 patients with biopsy-proven primary GN and 12 normal controls by radioimmunoassay and by copper-plated and cadmium column reduction assay, respectively. The results showed that urinary ET-1 levels in the patients with primary GN were significantly higher than in normal controls (p < 0.01), while the urinary ET-1 levels in patients with moderate mesangial proliferation GN were significantly higher than those in patients with mild mesangial proliferation GN (p < 0.05). Urinary ET-1 levels in patients whose clinical feature was nephrotic syndrome were found to be higher than in patients whose clinical feature was nephritic syndrome. However, urinary NO levels were to the contrary (p < 0.05). The ratio of ET-1/NO in primary GN patients was significantly higher than that in normal controls, and it positively correlated with the 24-hour urinary excretion of protein. These results suggest that urinary ET-1 levels are related to the proliferation of mesangial cells. The imbalance between ET-1 and NO may be related to the pathogenesis of primary GN and the occurrence of proteinuria.  相似文献   

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