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1.
目的 探讨细胞凋亡易感基因(CAS)可否作为肝癌的病理诊断标志物,以及肝细胞癌中CAS蛋白的表达与HBV感染之间的关系.方法 应用免疫组化法检测肝癌、癌旁组织及未发生肿瘤的肝硬化、肝炎组织中CAS蛋白的表达情况,同时应用免疫组化法、核酸原位杂交法检测HBV感染的肝细胞癌组织、癌旁组织中HBsAg、HBcAg以及HBV DNA的表达情况,分析肝细胞癌中CAS蛋白的表达与HBV感染之间的关系.结果 CAS蛋白在肝癌组织中的表达较癌旁组织明显升高(P<0.01),而癌旁组织中CAS蛋白表达较未发生肿瘤的肝硬化、肝炎组织显著增强(P<0.01).低分化型肿瘤细胞的CAS蛋白表达明显高于中分化型和高分化型(P<0.01).CAS蛋白在HBV感染的肝细胞癌组织中的表达明显高于非HBV感染的癌组织(P<0.01),其中HBV DNA阳性的肝细胞癌组织中CAS蛋白表达水平显著高于HBV DNA阴性的肝细胞癌组织(P<0.05).结论 CAS蛋白在肝细胞癌组织中呈高表达,肝细胞癌分化愈低其表达愈强,表明CAS蛋白可作为肝细胞癌的病理诊断与分化程度的评价标志物.并推测HBV DNA可能通过上调CAS的表达,在HBV感染相关性肝癌的发生发展过程中发挥重要的作用.  相似文献   

2.
为了探讨中国散发性阿尔茨海默病(SAD)颈动脉粥样硬化(CAS)与ApoE基因多态性的关系,应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测46名SAD患者、31名CAS患者及50名正常老年人的ApoE基因多态性分布特征。结果显示SAD组及CAS组ApoE ε4等位基因频率均高于对照组(P<0.05);SAD组为15.2%,CAS组为14.5%,对照组为3.0%,但CAS组ApoEε2等位基因频率明显高于SAD组(P<0.05);CAS组为41.9%,SAD组为21.7%本研究提示,ApoE4等位基因是SAD和CAS的共同危险因素,但ApoE2等位基因对CAS病人患AD具有保护作用。  相似文献   

3.
颈动脉粥样硬化(carotid atherosclerosis,CAS)与脑血管发病密切相关。目前研究认为颈动脉内-中膜厚度(carotid intima—media thickness,CIMT)是反映CAS的早期指标。本文综述CAS研究进展。  相似文献   

4.
针刺对高分子右旋糖苷所致脑软膜微循环障碍的影响   总被引:1,自引:0,他引:1  
本实验应用高分子右旋糖苷复制家兔脑软膜微循环障碍 ,选用激光多普勒微循环血流仪及多部位微循环观测系统观测脑微循环的改变。显示约 60 %家兔脑软膜微循环障碍。电针足三里、曲池及人中、内关可增加高分子右旋糖苷所引起微循环障碍后脑微循环血流量  相似文献   

5.
目的:检验认知评估系统(CAS)对中国学前儿童的使用效果,分析其信度和效度.方法:对三个班级105名幼儿园儿童进行先后两次CAS测验,同时施测麦卡锡儿童智能量表作为效标工具.结果:重测相关系数(r=0.73,P<0.001)显示CAS具有较高稳定性.CAS与麦卡锡儿童智能量表言语(r=0.36,P<0.01)、数量(r=0.53,P<0.01)、记忆(r=0.40,P<0.01)的关联效度良好.结论:CAS对中国学前儿童具有良好的适用性.  相似文献   

6.
目的:分析2型糖尿病(T2DM)合并颈动脉粥样硬化(CAS)患者外周血单个核细胞(PBMCs)中衔接蛋白凋亡相关斑点样蛋白(ASC)mRNA的表达及作用。方法:根据华盛顿大学CAS斑块超声分级标准将107例T2DM患者分为单纯T2DM组(n=26)、T2DM轻度斑块组(n=32)、T2DM中度斑块组(n=38)和T2DM重度斑块组(n=11)。另选非T2DM的CAS患者作为单纯CAS组(n=35),以及体检健康者作为正常对照组(n=35)。采用实时荧光定量聚合酶链反应(RT-FQ-PCR)技术检测各组PBMCs中ASC mRNA表达水平。统计分析各组差异及ASC mRNA水平与CAS斑块严重程度的关系。结果:各病例组ASC mRNA表达水平显著高于正常对照组(P0.05),T2DM合并CAS组ASC mRNA表达水平显著高于单纯CAS组(P0.01);ASC mRNA表达水平轻度斑块组中度斑块组重度斑块组,即ASC mRNA表达水平与T2DM患者CAS斑块程度呈明显负相关(r=-0.43,P0.05)。结论:ASC mRNA表达增加可能是T2DM合并AS的发病因素和CAS斑块活跃性的指征。  相似文献   

7.
<正>主办单位:中国微循环学会休克专业委员会、河北省中西医结合学会微循环专业委员会、湖北省微循环学会、微循环学杂志社、河北省医学会微循环-血液流变学分会、黑龙江省微循环血液流变学学会、内蒙古赤峰市医学会。承办单位:内蒙古医科大学赤峰临床学院(赤峰市医院)。  相似文献   

8.
《中国微循环》杂志是微循环、血液流变学、微血管医学专业学术刊物 ,国内外公开发行。主要阅读对象为从事微循环、血液流变学基础与临床研究、血栓性疾病研究、教学和临床工作者、大专院校师生及其他相关学科的科研、教学和临床工作人员。来稿范围 :1.微循环基础与临床研究成果、经验总结,微循环与微血管病的生理、病理生理和临床研究 ,微循环的调控机制 ,影响微循环内外因子研究 ,细胞、分子生物学与微循环 ,器官微循环 ,各种疾病的微循环 ,微循环在各类疾病发病与治疗中的作用 ,微循环与各类特殊环境的关系 ,特殊作业微循环 ,改善微循…  相似文献   

9.
1.微循环的现代概念:微循环是直接参与细胞、组织的物质、能量、信息传递的血液、淋巴液、组织液的流动,它包括血液微循环、淋巴微循环和组织微循环三个部分。目前血液微循环的研究较多,研究对象涉及人体和多种实验动物,研究部位包括体表组织和内脏器官;而淋巴微循环的研究相对较少,研究对象主要是实验动物;组织微循环(组织通道)的研究由于技术尚未成熟,所以少有报道。  相似文献   

10.
微循环病变是导致组织低灌注的关键环节,对微循环进行监测在重症疾病中非常重要。舌体富含微血管,其中舌下微循环呈现网状结构,一定程度上反映活体组织微循环状态,是进行临床微循环监测和活体动物微循环检测的理想和重要部位。综述舌下微循环显微影像监测的设备、指标体系、应用情况。首先,综述监测设备,包括设备组成、探头采用的光学技术种类、主机采用的图像处理算法、探头的固定形式;其次,归纳舌下微循环显微影像监测的指标体系,包括灌注质量指标、血管密度指标、灌注不均一性指标;然后,举例说明临床和实验应用情况,包括利用舌下微循环显微影像技术开展临床上疾病与微循环关联性的研究、药物与微循环关联性研究以及脏器微循环间的关联性研究。最后总结临床诊治和研究的意义,并对技术改进与发展、应用方向拓展进行展望。  相似文献   

11.
Electrical stimulation of the lateral hypothalamus resulted in electrocardiographic evidence of acute myocardial ischemia in 35% of normal adult rats under anesthesia. Mean arterial blood pressure was also elevated. Study of vascular corrosion casts disclosed that spasm of smaller branches of the coronary circulation, rather than the major epicardial arteries, was the main cause of the ischemic response. The histologic changes of the same experimental treatment in a separate group of animals revealed multiple focal areas of tissue damage throughout the myocardium, which were quantitatively assessed. The results may be relevant for the clinical problem of various forms of ischemic heart disease in which little evidence is found for organic (atherosclerosis) or dynamic (spasm) stenosis involving the major coronary arteries.  相似文献   

12.
Cocaine abuse has been associated with pathologic cardiovascular events including acute myocardial infarction (AMI) and sudden death. Although coronary vasospasm has been proposed as a possible mechanism, the ability of cocaine to induce coronary spasm has not been conclusively demonstrated. In these studies, isolated rat hearts were perfused with cocaine (100 micrograms to 500 micrograms/ml) for 1 minute, perfusion-fixed with glutaraldehyde, and histologically assessed for evidence of coronary spasm through light and electron microscopy. Light micrographs revealed that cocaine induced spasm in coronary arterioles up to 65 microns in diameter, whereas larger caliber vessels did not constrict. Ultrastructurally, vacuolation was observed in the endothelial and smooth muscle cells of constricted arterioles. Endothelial integrity was maintained and interendothelial junctions remained intact. Morphologic evidence of constriction was supported by data obtained from Langendorff-heart preparations in which cocaine reduced myocardial flow rate under constant pressure conditions and increased aortic perfusion pressure under constant flow conditions. Spasm induced by cocaine was prevented by the calcium entry blocker nitrendipine, but not by phentolamine, an alpha-adrenergic antagonist. The finding of small vessel spasm in this study may explain the significant number of clinical cases of cocaine-associated AMI in which the main coronary arteries appear angiographically normal.  相似文献   

13.
目的 :建立活体心脏微循环的观测方法 ,研究猪体外循环围术期冠状微循环的变化规律 ,探讨其临床意义。方法 :小型猪 12头建立体外循环 ,心脏停跳 45min ,再灌注 12 0min。以右心耳的心外膜作为微循环观测窗。分别于体外循环转流前、心脏停跳前、主动脉开放后 5、3 0、60和 12 0min六个时间点 ,用接触式显微镜连接在电视监测器屏幕上观测冠状微血管形态和微血流的动态变化 ;应用激光多普勒微循环血流计测定心肌微区灌流量。结果 :猪体外循环心肌再灌注后 5~60min ,冠状微动脉口径较缺血前显著缩小 (P <0 .0 1) ,微血管中出现搏动性颗粒状血流 ,并有血浆与血细胞分离现象 ,提示有明显的红细胞聚集与毛细血管前括约肌痉挛。再灌注 5~ 3 0min ,心肌微区灌流量亦较缺血前下降 (P <0 .0 5 )。结论 :体外循环心肌缺血 再灌注后出现了冠状微血管痉挛、微血流瘀滞和心肌微区灌流量的下降 ,冠状微循环障碍与体外循环术后心功能不全有一定的相关关系。  相似文献   

14.
冠状动脉微栓塞致心肌损伤研究进展   总被引:1,自引:1,他引:0       下载免费PDF全文
冠状动脉微栓塞是急性心肌梗死(acute myocardial infarction)急诊经皮冠状动脉介入治疗(percutaneous coronary intervention)过程中常见的并发症,发生率约10%-20%,不仅导致“慢血流”(slow flow)或“无再流”(no flow),更为重要,它是影响冠状动脉微心肌微循环灌注的主要原因,是AMI患者远期预后不良和死亡率的独立预测因子。  相似文献   

15.
Coronary artery spasm (CAS), an intense vasoconstriction of coronary arteries that causes total or subtotal vessel occlusion, plays an important role in myocardial ischemic syndromes including stable and unstable angina, acute myocardial infarction, and sudden cardiac death. Coronary angiography and provocative testing usually is required to establish a definitive diagnosis. While the mechanisms underlying the development of CAS are still poorly understood, CAS appears to be a multifactorial disease but is not associated with the traditional risk factors for coronary artery disease. The diagnosis of CAS has important therapeutic implications, as calcium antagonists, not β-blockers, are the cornerstone of medical treatment. The prognosis is generally considered benign; however, recurrent episodes of angina are frequently observed. We provide a review of the literature and summarize the current state of knowledge regarding the pathogenesis of CAS.  相似文献   

16.
In the brain death baboon model, the baboon experiences an autonomic storm with release of catecholamines both from the adrenal glands and from intracardiac sympathetic nerve endings. Since catecholamines may induce coronary arterial spasm, we looked for morphologic evidence of smooth muscle damage in the coronary arteries of 11 baboons that underwent induction of brain death under general anesthesia. Nine (82%) of the 11 animals showed coronary arterial smooth muscle contraction bands, and 9 (82%) of the 11 baboons also showed focal myocardial contraction bands and myocytolytic necrosis. Focal coronary arterial smooth muscle cell necrosis with intracytoplasmic calcium deposits were observed in three (33%) of the nine contraction band-positive cases. This study provides further support for the concept that medial smooth muscle contraction bands may be a morphologic marker of antemortem coronary arterial spasm.  相似文献   

17.
A review of the evidence in acute myocardial infarction indicates that it might well be initiated by coronary artery spasm, and that coronary thrombosis, although important in tipping the balance between severe damage and death of heart muscle, may merely be secondary to the spasm-induced reduction in coronary blood flow.  相似文献   

18.
Summary In arterial hypertension left ventricular hypertrophy comprises myocyte hypertrophy, interstitial fibrosis and structural alterations of the coronary microcirculation. This leads to an impairment of diastolic function of the left ventricle and coronary flow reserve despite normal epicardial arteries. Consequently, antihypertensive treatment should aim at [13] reversing myocyte hypertrophy, [14] restoring myocardial structure and [8] improving coronary flow reserve along with blood pressure normalization.  相似文献   

19.
There is no published registry for drug-induced acute myocardial infarction (AMI) with subsequent patent coronary angiogram in teenagers. To highlight the mechanism and impact of drug-induced MI with patent coronary arteries among teenagers who have relatively few coronary risk factors in comparison with older patients, we conducted a review of the literature. In this review most of the pertinent published (English and non-English) articles through the Medline, Scopus, Cochrane Database of Systematic Reviews and EBSCO Host research databases from 1970 to 2005 have been revised. Teenagers and young adults with AMI and subsequent patent coronary angiogram were included. In those cases drug-induced coronary spasm was highlighted. Among 220 articles (>12000 cases) related with AMI with normal coronary angiogram, 50 articles (approximately 100 cases) reported the role of drug in AMI secondary to coronary artery spasm (CAS). There is no well-conducted trial for AMI secondary to CAS in young adults but only a series of case reports and the diagnosis in most of cases was based on the clinical and laboratory findings without provocation. CAS was associated with 12 illicit substances in teenagers (i.e, cocaine, marijuana, alcohol, butane and amphetamine). Smoking is not only the initiative but also might harbor other illicit substances that increase the risk for CAS. Cocaine-associated AMI is the most frequent in various research papers. CAS was reported with 19 types of medications (i.e, over-the-counter, chemotherapy, antimigraine and antibiotics) without strong relation to age. Despite drug-induced AMI being not a common event, attention to smoking and drugs in teenagers and young adults will have major therapeutic and prognostic implications.  相似文献   

20.
择期经皮冠脉介入术对急性心肌梗死心肌微循环的作用   总被引:1,自引:0,他引:1  
目的应用心肌声学造影(MCE)评价急性心肌梗死(AMI)患者择期经皮冠脉介入术(PCI)前后的心肌微循环。方法选择20例AMI患者,在择期PCI治疗前、后分别应用声诺维(SonoVue)静脉注射,行间歇触发、二次谐波MCE检查,应用声学密度分析软件(AD)定量测定心肌微循环内造影剂的声学峰值强度(PI)、曲线下面积(AUC)。结果治疗前梗死相关节段的PI、AUC明显低于正常灌注节段(P<0.001);择期PCI治疗后梗死节段的PI、AUC仍明显低于正常节段(P<0.001),但较PCI前明显升高(P<0.001),校正后的PIr、AUCr亦明显升高(P<0.001);而正常灌注节段的PI、AUC在PCI前后无明显变化(P>0.05)。结论MCE可定量评定AMI患者的心肌微循环;择期PCI可改善AMI患者梗死节段的微循环。  相似文献   

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