系数倍率分光光度法在三组分复方扑热息痛片含量测定中的应用

本文介绍了系数倍率分光光度法用于三组分体系测定的基本原理和实验方法,用该法在7520型分光光度计上测定复方扑热息痛片中阿斯匹林、扑热息痛和咖啡因的含量。本文借助于微处理机选择最佳测定波长,不经分离直接测定四个波长的吸收值,可同时分别测得三组分的含量。方法简便快速,其平均回收率;阿斯匹林为99.6±0.36%;扑热息痛为100.0+0.37%;咖啡因为100.6±0.74%(95%置信率)。     

系数倍率分光光度法在三组分复方扑热息痛片含量测定中的应用

本文介绍了系数倍率分光光度法用于三组分体系测定的基本原理和实验方法,用该法在7520型分光光度计上测定复方扑热息痛片中阿斯匹林、扑热息痛和咖啡因的含量。本文借助于微处理机选择最佳测定波长,不经分离直接测定四个波长的吸收值,可同时分别测得三组分的含量。方法简便快速,其平均回收率;阿斯匹林为99.6±0.36%;扑热息痛为100.0+0.37%;咖啡因为100.6±0.74%(95%置信率)。     

紫外分光光度法和高效液相色谱法测定吡喹酮缓释片的溶出度

采用紫外分光光度法和高效液相色谱法对吡喹酮缓释片的溶出度进行了测定.两种方法的测定结果基本一致。2h 溶出度是25.27±6.04%(UV)和25.80±5.49%(HPLC),3h 溶出度是72.80±4.21%(UV)和77.32±2.96%(HPLC),5h 溶出度是91.69±3.43%(UV)和92.41±4.16%(HPLC)。     

紫外分光光度法和高效液相色谱法测定吡喹酮缓释片的溶出度

采用紫外分光光度法和高效液相色谱法对吡喹酮缓释片的溶出度进行了测定.两种方法的测定结果基本一致。2h 溶出度是25.27±6.04%(UV)和25.80±5.49%(HPLC),3h 溶出度是72.80±4.21%(UV)和77.32±2.96%(HPLC),5h 溶出度是91.69±3.43%(UV)和92.41±4.16%(HPLC)。     

PLS-紫外分光光度法同时快速测定增效联磺片中三组分含量

本文应用偏最小二乘法(PLS)测定了增效联磺片中SMZ,SD和TMP的含量。同时提出了一种新的溶剂系统。所得三组分的平均回收率分别为99.47±0.81%、100.84±0.73%和99.49±0.74%(置位度95%)。与常规湿化学法相比,PLS法的运行时间短,结果可靠,尤其适用于批量处理。     

双波长分光光度法测定脉路通片中潘生丁的含量

脉路通片内含潘生丁和阿斯匹林。因阿斯匹林的存在使潘生丁的含量测定受到干扰。本文报告了采用日立200—20型单波长双光束分光光度计,按双波长法选择λ_2=288nm,λ,=260nm,样品不经分离,直接测定潘生丁的含量,得到满意的结果。方法简单、准确,用给出的条件,测定八份样品,平均回收率99.2±0.5%。     

紫外分光光度法测定甲硝唑漱口液含量

采用紫外分光光度法直接测定甲硝唑漱口液中甲硝唑含量,以水为溶剂,(319±1)nm为测定波长.甲硝唑在2～20μg/ml范围内,浓度与吸收度的线性关系良好(r=0.9999),平均回收率为100.1%,RSD为0.82%(n=6).本法简便、快速、准确,适用于该制剂的含量测定.     

西咪替丁在它的酸裂解产物存在时的分光光度测定法

介绍两种测定西咪替丁的方法,即西咪替丁在它的酸裂解产物存在时用UV法(方法Ⅰ)测定第二衍生物和应用2,6-二氯苯酚-靛酚比色法(方法Ⅱ)测定。两种方法的相对标准偏差分别为0.75%和0.72%。两种方法测定西咪替丁片剂的平均标准偏差各为100.5±0.68%和100.1±0.92%。测定安瓿时的平均标准偏差各为99.5±0.77%和100.1±0.79%。标准溶液1.西咪替丁200μg/ml(标准A)准确称20mg西咪替丁,用20ml0.1mol/L盐酸     

治疗痤疮新药益肤酰胺的含量测定

本文采用了非水滴定法和紫外分光光度法测定了益肤酰胺的含量。紫外分光光度法方法简便,是一个比较理想的含量测定方法。本文同时用五台不同型号的分光光度计于290±1nm 波长处测定了浓度为10.04×10~(-4)—40.16×10~(-4)g/ml样品溶液的吸收度,并确定了百分吸收系数E_1(?)为498.95(n=44)、标准差为1.995;变异系数为0.40%。     

紫外分光光度法测定枸橼酸盐的含量

本文采用紫外分光光度法测定枸橼酸盐制剂中枸橼盐的含量,方法简单,效果满意,适用于临床常用的多种枸橼酸盐制剂的含量检测.枸橼酸盐与铜离子生成稳定的络合物在241±1nm波长测定,平均回收率100.1%,变异系数为2.12%,(n=8).     

Dissolution and assaying of multicomponent tablets by chemometric methods using computer-aided spectrophotometer

Dissolution of three component tablets containing paracetamol (APAP), propyphenazone (PP), and caffeine (CAF) was carried out by USP paddle method. Three chemometric methods; inverse least square (ILS), principal component regression (PCR) and partial least squares (PLS) were applied to simultaneous assay of APAP, PP and CAF in tablets. The PCR, PLS and ILS methods were applied to simultaneous dissolution APAP, PP and CAF in tablets using a double beam UV-Vis spectrophotometer without any chemical separation and any graphical treatment of the overlapping spectra of three drugs. Twenty two mixture solutions in different concentrations were prepared in simulated gastric juice (SGJ, USP) for the chemometric calibrations as training set. The absorbance data matrix was obtained by measuring the absorbance at 14 wavelength points (from 222.5 to 292.5 nm) with the intervals of 5 nm (Deltalambda=5 nm) in the spectral region between 200 and 310 nm. Training set and absorbance data were used for the calibrations of chemometric methods. The developed calibrations were tested for the previously prepared solutions of mixture of three drugs for the validation of the assay method. The chemometric calculations were performed by using the 'MAPLE VRSQUO; software. The results of three chemometric methods were statistically compared with each other. These chemometric calibrations were successfully applied to the content uniformity and dissolution of the multicomponent tablets without any separation procedure. The synthetic mixtures of three drugs were used for the validity of the calibrations. Means recoveries (percent) and relative standard deviation of PLS, PCR and ILS methods were found to be 100.1+/-0.6, 101.4+/-1.6 and 100.1+/-0.6 for APAP; 100.9+/-3.2, 102.0+/-3.3 and 100.9+/-3.2 for PP; 99.9+/-3.5, 101.6+/-3.3 and 99.9+/-3.2 for CAF, respectively. Dissolution profiles of three component tablets were performed. More than 95% of drugs were dissolved within 15 min. All of the three-chemometric methods in this study can be satisfactorily used for the quantitative analysis and for dissolutions test of multicomponent dosage form.     

岭回归分光光度法同时测定复方阿司匹林片中的各组分

本文将岭回归用于分光光度分析中的多组分同时定量,着重介绍了方法的基本原理,基本步骤和基本计算。指出了它特别适用于最小二乘估计不够理想的情况,即当吸收系数矩阵S=ε^Tε接近退化,以致使浓度值C接近不可估的病态体系。并将该法用于复方阿司匹林片中三种组分的测定,取得满意结果。     

Quantitative NMR assay for aspirin, phenacetin, and caffeine mixtures with 1,3,5-trioxane as internal standard

The method for (1)H NMR determination of aspirin, phenacetin and caffeine (APC) mixtures has been improved by the use of 1,3,5-trioxane as an internal standard. The trioxane absorption occurs in a peak-free region of the spectrum and produces no interferences with any of the analytes. Compared to the original method with caffeine as an external standard, the present method appears to offer better accuracy and precision. Average errors relative to the correct results were: aspirin, 1.0%; phenacetin, 0.8%; and caffeine 1.8%, for known standard mixtures. Coupling constants, (1)J((13)CH), were determined for the methyl groups of aspirin and caffeine and for the trioxane methylene group to clarify potential (13)C satellite interferences.     

多组分计算分光光度分析-AKC法与CPA法的比较研究

在分光光度分析中,用矩阵表达进行多组分同时定量计算时,将比尔定律表示成矩阵形式的方法通常叫AKC法,而将浓度做为吸收度的函数的矩阵表示方法叫CPA法。本文用复方阿司匹林片剂对两种方法进行了比较研究,并用〔det(K_TK)〕_(1/2)做为灵敏度指标,指导确定波长间隔和波长点数,获得非常满意的结果。     

因子分析分光光度法及其应用研究

本文用目标检验因子分析-分光光度法对阿司匹林,非那西丁,咖啡因3组分混合体系进行了定性和定量研究。用该方法确定了体系中存在的组分数,并测定了各组分的含量。对12个模拟样品的计算结果表明,该方法的特点是既能鉴定组分数,又能进行大批量试样的同时测定。     

Quantitative comparison of the analgesic and anti-inflammatory activities of aspirin, phenacetin and acetaminophen in rodents.

The mild analgesic activities of aspirin, phenacetin and acetaminophen have been compared in the trypsin, kaolin and carrageenan hyperalgesic assays as well as in the acetic acid writhing test. The trypsin and kaolin hyperalgesic assays were designed to be unaffected by drugs with anti-inflammatory activity. Aspirin and acetaminophen were inactive in these two tests at dose levels devoid of side effects. Phenacetin was active in the trypsin and kaolin assays with oral ED50's of 114 +/- 36.2 and 107 +/- 11.5 mg/kg, respectively. Non-steroidal anti-inflammatory drugs as well as phenacetin and acetaminophen were active in the acetic acid writhing and carrageenan hyperalgesic assays. This led to evaluation of phenacetin and acetaminophen as anti-inflammatory agents. Both of these latter drugs were active in the carrageenan pleurisy and adjuvant arthritis models of inflammation. In all studies phenacetin was equipotent to or more potent than acetaminophen. The data suggest that the analgesia produced by aspirin and acetaminophen results from their anti-inflammatory activity whereas the analgesia produced by phenacetin has two components, one dependent on and one independent of anti-inflammatory activity.     

Determination of salicylic acid and aspirin in multicomponent tablets by liquid chromatography on a nonionic resin

Aspirin and free salicylic acid were determined in combinations containing caffeine, phenacetin, salicylamide, and acetaminophen by liquid chromatography on poly(methyl methacrylate) resin. Precision and accuracy were +/- 2% for salicylic acid, the latter at levels corresponding to 0.02% of the aspirin content.     

偏最小二乘紫外分光光度法同时测定克感敏片中三种成分的含量

介绍了偏最小二乘法在分光光度法中同时测定复方药物的原理。用该法对克感敏片中三种成分非那西汀、氨基比林和咖啡因的含量进行了同时测定,所得平均回收率为100.2±0.4%,99.6±0.6%和100.3±0.7%(置信度为95%)。     

Effects of phenacetin,paracetamol and caffeine on the erosive activity of acetylsalicylic acid in the rat stomach: dose-response relationships,time course of erosion development and effects on acid secretion

Adult male and female Wistar rats were equally susceptible to gastric injury induced with acetylsalicylic acid (aspirin). Both in male and in female rats simultaneous administration of caffeine and aspirin caused significantly more gastric erosions than the same dose of aspirin alone; likewise addition of paracetamol to aspirin decreased the incidence of gastric lesions in either sex, and addition of phenacetin to aspirin had no effect. The potentiation by caffeine and the inhibition by paracetamol were both dose-dependent and only markedly influenced the development of erosions after 3–4 h. Pretreatment with phenacetin or paracetamol 1 h before administration of aspirin did not affect its erosive activity. Administration of benorylate caused no more gastric erosions than the vehicle or than equivalent mixtures of aspirin and paracetamol. The histamine-stimulated acid output of the stomach during gastric perfusion with aspirin was rapidly diminished. Neither paracetamol nor caffeine initially affected this decrease in acid output. However, 30 min after perfusion with aspirin and caffeine, acid secretion increased approximately as strongly as after caffeine alone. Caffeine potentiates aspirin-induced erosions by its stimulatory effect on acid secretion whereas paracetamol inhibits these erosions by preventing their growth.     

New Chemometrics Mode Based on Adjacent Data Points’ Differences for the Simultaneous Determination of Clopidogrel,Atorvastatin, and Aspirin in their Combined Ternary Drug Formulation

A new method is proposed for the analysis of a ternary mixture composed of clopidogrel, atorvastatin, and aspirin without prior separation steps. The method combines the advantages of the mean centering of ratio spectra and derivative spectrophotometric methods. It is based on using the difference between adjacent data points in the absorbance spectra. The principal advantage of this method is the use of absorbance data, and not derivative data; hence the signal-to-noise ratio is not diminished. The mathematical explanation of the procedure is illustrated. Beer’s law was valid in the concentration range 0.3–35 μg.mL^-1 for CLOP, 0.5–30 μg.mL^-1 for ATOR, and 1–40 μg.mL^-1 for ASP. Mean recoveries were obtained as 100.2, 100.1, and 100.2% for CLOP, ATOR, and ASP, respectively, in the prepared synthetic mixtures. The method has been successfully applied to the simultaneous determination of ternary mixtures of aspirin, clopidogrel bisulphate, and atorvastatin calcium. The analytical characteristics of the method were calculated. The results showed that the new method is simple, rapid, accurate, and precise.