精子顶体酶活性与精液常规检查参数关系分析

目的通过比较精子顶体酶正常组和异常组精液常规检测参数差异,明确顶体酶活性在评估男性生育能力中的重要意义。方法从316例男性不育症患者的精液中选取36例顶体酶活性正常标本和36例顶体酶活性异常标本,分别作为顶体酶活性正常组和顶体酶活性异常组。所有精液标本均进行精子活率、A＋B级精子活力、精子密度、精子畸形率、精予头部畸形率测定,运用相关统计学方法分析。结果顶体酶正常组精子活率、A＋B级精子活力、精子密度均显著高于异常组,精子畸形率和精子头部畸形率均显著低于异常组,且精子顶体酶活性与精子活率、A＋B级精子活力、精子密度呈显著正相关与精子畸形率和精子头部畸形率呈显著负相关。结论精子顶体酶活性可以代表精子质量,精液常规检测参数可能是精子顶体酶活性的影响因素,可作为临床评估男性生育能力的重要指标。     

冷冻对精子顶体酶活性及精子功能影响的研究

目的:探讨精子冷冻对精子功能和精子顶体酶活力的影响.方法:选取来自正常生育男性志愿者,且精液常规分析各参数正常的精液为标本,检测精子冷冻前后精液常规各参数、精子功能和精子顶体酶活性.结果:冷冻后精子存活率54.96±6.00％,精子宫颈黏液穿透能力1.70±0.47,精子顶体酶活性15.31±8.87mU/百万精子,与冷冻前精子存活率65.74±6.62％,精子宫颈黏液穿透能力2.65±0.49,精子顶体酶活性54.32±36.38mU/百万精子比较显著下降(P＜0.05).结论:精子冷冻导致精子膜功能和精子穿透功能受损,引起精子顶体酶活性显著下降.     

男性不育患者精液活性氧与精子参数的关系研究

目的研究男性不育患者精液活性氧(ROS)与精子参数的关系,为男性不育诊疗提供依据。方法选取2013—2014年1月在嘉兴市妇幼保健院生殖医学中心进行精液检测的758例男性不育患者,采用化学发光法检测精液ROS值。根据精液检查结果及世界卫生组织(WHO)标准,分析精子总数、精子密度、前向运动精子比率(PR)和白细胞含量等精子质量指标,及与ROS水平的关联性。结果精子总数39×106组患者ROS平均值为(10.5±3.1)RLU/S,高于精子总数≥39×106组的(9.2±2.2)RLU/S(P0.05)。精子密度≥15×106/m L组和15×106/m L组ROS平均值分别为(9.4±2.5)RLU/S和(9.3±2.9)RLU/S,两组差异无统计学意义(P0.05)。PR≥32%组和PR32%组ROS平均值分别为(9.4±2.2)RLU/S和(9.3±2.7)RLU/S,两组差异无统计学意义(P0.05)。白细胞含量≥1.0×106/m L组患者ROS平均值为(113.0±34.7)RLU/S,高于白细胞含量1.0×106/m L组患者的(9.0±2.6)RLU/S(t=6.569,P0.05)。结论精液ROS水平升高可引起精子数量减少,白细胞数量异常增加会引起精液ROS水平升高。     

冷冻精液室温暴露时间对前向运动精子冷冻复苏率的影响

目的:研究冷冻精液在室温下反复暴露时间对复苏精液质量的影响。方法:选取2018年广东省计划生育专科医院人类精子库筛查期10例供精者的40份精液标本,按照精液在室温(26℃)下暴露时间长短分为1min组、2min组、4min组、对照组(未暴露于室温),分别检测反复在室温下暴露20次的不同组别的冷冻精液复苏前后的前向运动精子百分率,并计算前向运动精子冷冻复苏率。结果:对照组前向运动精子冷冻复苏率[(73.84±2.53)%]显著高于2min组[(71.15±.21)%]、4min组[(67.65±0.94)%](P0.05),与1min组[(72.83±0.79)%]无差异(P0.05);1min组前向运动精子冷冻复苏率显著高于2min组(P0.05)和4min组(P0.05);2min组前向运动精子冷冻复苏率显著高于4min组(P0.05)。结论:冷冻精液在室温下反复暴露≥2min对前向运动精子冷冻复苏率有显著影响,且冷冻精液在室温暴露时间越长,前向运动精子冷冻复苏率越低。冷冻精液在室温下反复暴露≤1min对前向运动精子冷冻复苏率无显著影响。     

全面二孩政策实施后准备生育二孩的男性精液质量分析

目的 对二孩政策全面实施后准备生育二孩的丈夫精液常规参数、精子畸形率进行分析。 方法 将纳入标准的研究对象分为意向生育二孩组(二孩组,n=232)、试管婴儿组(试管组,n=120)、正常生育组(正常组, n=82),精液标本充分液化后严格按照《WHO人类精液检查与处理实验手册(第五版)》进行精液常规分析, 采用改良巴氏染色法, 严格按照Kruger标准检测研究对象精子畸形率。 结果 ① 二孩组、试管组、正常组的年龄分别为(37.1±5.7)、(33.0±6.2)、(28.2±3.8)岁;精液量分别为(2.8±1.7)ml、(2.8±3.5)ml、(3.1±0.9)ml;精子浓度分别为(73.4±53.6)×10⁶/ml、(45.7±49.7)×10⁶/ml、(79.8±48.0)×10⁶/ml;精子总活力分别为(60.9±18.8)%、(30.7±13.0)%、(71.8±14.8)%;精子前向运动率分别为(51.2±17.9)%、(23.5±10.8)%、(61.9±15.9)%;精子畸形率分别为(87.8±5.8)%、(93.6±6.1)%、(86.5±6.7)%。② 三组间进行两两比较,二孩组的精子浓度、精子总活力、精子前向运动率和正常形态精子百分比方面明显优于试管组,差异有统计学意义(P＜0.01),精液量均值与试管组相同,但差异有统计学意义(P＜0.05);与正常组相比,二孩组在精液量、精子总活力、精子前向运动率方面低于正常组,差异有统计学意义(P＜0.01),但精子浓度、精子总畸形率方面差异无统计学意义(P＞0.05);正常组在精液量、精子浓度、精子总活力、前向运动率、精子形态方面明显优于试管组,差异有统计学意义(P＜0.01)。 结论 准备生育二孩的男性精液质量较正常生育者差异不明显,但要明显优于进行试管婴儿的男性。     

精液参数及精子来源对妊娠结局的影响

目的探讨精液参数及精子来源对卵胞浆内单精子注射(ICSI)妊娠结局的影响。 方法选择2009年1月至2013年12月,在内蒙古医科大学附属医院妇产科生殖医学中心接受ICSI治疗的303对不育夫妇,共计324个周期的临床病历资料为研究对象。根据取卵当日精液参数及精子来源不同,将这324个ICSI周期分为正常精液组(精液参数及精子来源正常,56个周期)和研究组(精液参数或精子来源异常,268个周期),其中精液参数异常包括极度少精子症亚组(43个周期),极度弱精子症亚组(21个周期),极度少、弱精子症亚组(33个周期)及中/重度少、弱精子症亚组(113个周期);精子来源异常包括睾丸穿刺精子亚组(25个周期)及附睾穿刺精子亚组(33个周期),共计6个研究亚组。回顾性分析324个周期的临床病历资料,分别将上述6个研究亚组的受精率,正常受精率,卵裂率、可移植胚胎率、优质胚胎率、临床妊娠率及胚胎种植率与正常精液组进行统计学分析。7组(6个研究亚组及正常精液组)受试者之间年龄、不孕年限、获卵数、MⅡ卵率及平均移植胚胎数等一般资料比较,差异无统计学意义(P>0.05)。本研究遵循的程序符合内蒙古医科大学附属医院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书。 结果①受精率比较：极度少精子症亚组,极度少、弱精子症亚组,睾丸穿刺精子亚组及附睾穿刺精子亚组分别为65.1%,78.4%,71.8%及76.6%,均显著低于正常精液组的81.0%,且差异均有统计学意义(χ²＝48.349,P＝0.000;χ²＝5.800,P＝0.016;χ²＝19.884,P＝0.000;χ²＝9.570,P＝0.002)。②正常受精率比较：极度少精子症亚组,极度少、弱精子症亚组,睾丸穿刺精子亚组及附睾穿刺精子亚组分别为60.0%,72.0%,65.6%及69.0%,均显著低于正常精液组的79.0%,且差异有统计学意义(χ²＝38.945,P＝0.000;χ²＝6.109,P＝0.013;χ²＝17.315,P＝0.000;χ²＝11.107,P＝0.001)。③6个研究亚组的卵裂率、可移植胚胎率、优质胚胎率、临床妊娠率及胚胎种植率分别与正常精液组比较,差异均无统计学意义(P>0.05)。 结论极度少精子、睾丸穿刺精子及附睾穿刺精子,虽然可显著降低卵子受精率,但对胚胎种植率及临床妊娠率影响不显著。故通过ICSI治疗,可使因男性因素导致的不孕患者达到妊娠目的。     

50Hz工频磁场暴露对成年男性精子活力参数和精液酸碱度的影响

目的 研究50 Hz极低频磁场暴露对成年男性精子活力、活动力参数和精液酸碱度的影响.方法 将成年健康男性新鲜精液标本分别暴露于0.4 mT、50 Hz正弦磁场中15、30、60 min,采用WUY-9000型伟力彩色精子质量检测系统动态记录精子活力、活动力参数并测定精液酸碱度.结果 新鲜精液标本暴露于50 Hz磁场中15 min,可使精子活力(a+b级精子)和精子活动率(a+b+c级精子)均呈抑制状态,与平行对照组相比,差异有统计学意义(P＜0.01);暴露30 min,两组间精子活力和精子活动率差异无统计学意义(P＞0.05);暴露60 min后,精子活力和活动率又呈抑制状态,与平行对照组相比,差异有统计学意义(P＜0.01,P＜0.05).然而,相同参数的磁场暴露15、30、60 min,对精液酸碱度无明显影响.结论 暴露于0.4 mT、50 Hz的正弦磁场中不会影响男性精液的酸碱度,但可明显抑制精子的活力和活动率.     

对羟基苯甲酸丁酯通过氧化应激导致人精子活力降低和凋亡增加

目的探究对羟基苯甲酸丁酯(BP)对人精子活力、氧化应激和细胞凋亡的影响及其机制。方法选取人精液样本20份,随机分为4组:对照组及BP低、中、高浓度组,每组5个平行样。与BP体外共培养4 h,BP染毒浓度分别为0、200、400和800μmol/L。观察精子活动力、细胞毒性、活性氧阳性细胞率和细胞凋亡率。结果BP低、中、高浓度组精子总活力[(26.44±7.83)%、(32.79±2.90)%和(10.51±5.88)%]均低于对照组[(47.67±3.93)%],差异有统计学意义(P0.05)。与对照组相比,BP低、中、高浓度组精子存活率分别为(63.36±9.08)%、(49.72±7.15)%和(29.91±5.93)%。BP低、中、高浓度组活性氧阳性细胞率[(24.67±0.50)%、(54.50±3.40)%和(59.93±3.47)%]高于对照组[(8.63±0.57)%],差异有统计学意义(P0.05),晚期精子凋亡率[(11.8±1.74)%、(12.87±0.25)%和(14.60±0.87)%]高于对照组[(9.63±1.00)%],差异有统计学意义(P0.05)。精子活性氧的水平和晚期凋亡率均与精子的总活力呈负相关,r分别为-0.727和-0.688(P0.05)。结论对羟基苯甲酸丁酯具有细胞毒性,能够降低精子活动能力,促进精子活性氧的生成和细胞凋亡。     

睡眠时间对精液常规参数、精子形态及精子DNA完整性的影响

目的探讨睡眠时间对精液参数、精子形态以及精子DNA完整性的影响。方法对581名男性的睡眠情况进行问卷调查,将其分为三组,即Ⅰ组,正常睡眠组;Ⅱ组,轻度睡眠缺乏组;Ⅲ组,严重睡眠缺乏组。采用计算机辅助精液分析系统(CASA)检测精液密度、活力、前向运动率等常规参数;采用Diff-Quick染色法分析精子形态;采用精子染色质扩散实验(SCD法)分析精子DNA完整性。结果Ⅰ、Ⅱ、Ⅲ组精液密度(70.5±14.2、65.7±11.2、53.8±12.6)×106/ml、活力(74.3±10.1、70.5±14.5、65.8±12.8)%、前向运动率(57.2±9.5、54.5±11.3、48.3±13.7)%均呈下降趋势,差异有统计学意义(P0.01),并且Ⅱ、Ⅲ组精子畸形率(91.0±20.5、94.1±21.7)%和精子DNA碎片指数(17.5±10.1、24.6±11.7)%与Ⅰ组(86.4±18.3、16.8±9.5)%相比较,差异有统计学意义(P0.05)。结论睡眠时间影响男性精液质量,可能是导致男性精液质量下降的因素之一。     

少弱精症患者精子顶体酶活性分析

目的:研究少弱精症不育男性精子顶体酶活性变化与其生育力的关系。方法:以159例少弱精症和25例正常生育男性为研究对象,应用改良Kennedy法检测精子顶体酶活性,分析不同精子密度、活力、形态与顶体酶活性的关系。结果:正常组的顶体酶活性高于不同精子密度不育各组(>50×106ml、20～50×106ml、<20×106ml)(F=7.504,P<0.001);不同精子密度不育各组之间顶体酶活性比较,>50×106ml组顶体酶活性高于<20×106ml组(P<0.05),其它各亚组两两比较顶体酶活性无统计学差异;少弱精症不育者的精子畸形率与顶体酶活性呈负相关(r=-0.171),a+b级精子活力(r=+0.142)和顶体完整率(r=+0.154)与顶体酶活性呈正相关。结论:顶体酶活性对少弱精症男性生育力评价有重要价值,顶体酶活性可以作为精子形态分析之外重要的预测精子受精能力的指标。     

Effect of Caffeine on Human Sperm Penetration into Zona-Free Hamster ova

The effect of caffeine on spermatozoal ability to penetrate zona-free hamster ova was examined on fresh and frozen-thawed semen samples. The mean motility of 10 fresh semen samples incubated with caffeine significantly increased from 29% to 35%. Sperm penetration into zona-free hamster ova did not differ between the control group and the specimens to which caffeine was added. The same effect of caffeine on sperm motility and hamster ova penetration was noted in the frozen-thawed sperm samples. Motility was enhanced by 21%, but hamster ova penetration did not significantly change. The increase in sperm motility caused by caffeine does not change the fertilizing ability of fresh and frozen-thawed human sperm.     

Contraceptive potentiality of STS-557--a feasibility study in male bonnet monkey (Macaca radiata)

STS-557 (17 alpha-cyanomethyl-17 beta-hydroxy-estra-4, 9(10)-diene-3-one) was administered (i.m.) to two groups (4 in each group) of adult male bonnet monkeys at a daily dose of 10 mg/monkey for 12 weeks (first group) and 5 mg/monkey for 14 weeks (second group). Treatment with the 10 mg dose resulted in a significant decline in the count, motility, acrosin and hyaluronidase activities and the fertilizing ability (zona-free hamster egg penetration assay) of spermatozoa by the 6th week of initiation of treatment. The circulating level of STS-557 was low after one week and increased from the 2nd week of treatment when the serum level of testosterone was significantly reduced. Complete recovery was observed by the 11th week after withdrawal of treatment. The treatment with the 5 mg dose had minor and inconsistent effect on the motility, hyaluronidase and acrosin activity, and the fertilizing ability of spermatozoa in addition to the blood level of testosterone. STS-557 may have the potentiality to be used as a chemical contraceptive in the male but compensation for the reduced level of blood testosterone may be necessary.     

Cut-off value of reactive oxygen species for predicting semen quality and fertilization outcome

Though the role of reactive oxygen species (ROS) in male infertility is widely investigated worldwide, there is a lack of adequate information on the cut-off value of ROS beyond which ART outcome may be adversely affected. The objective of the present study is to establish an upper level of ROS in semen samples which can be considered as a potential marker of good semen quality. Semen ejaculates were randomly collected from 338 male partners of infertile couples. The upper critical limit (UCL) of ROS was calculated from the control chart of normozoospermic patients and found to be 0.75x10(6) counted photons per minute (cpm)/10 million cells. This was further validated by estimating ROS in 17 proven fertile men and 38 patients undergoing intra cytoplasmic sperm injection (ICSI). As expected, all abnormal semen samples exhibited higher ROS as compared to nomozoospermic and proven fertile samples. All semen samples from proven fertile volunteers were found to be <0.075x10(6) cpm/10 million cells. On the basis of the calculated UCL, ICSI patients were divided into two groups: Group I (UCL). The semen parameters, fertilization rate and pregnancy outcome were found to be significantly affected in Group II. Significant difference in live birth-rates was observed between Group I (47.6%) and Group II (17.6%) while no live-birth was recorded for ROS level >0.1x10(6) cpm/10 million cells. It is concluded that the upper cut-off value of normal semen samples that correlates with good semen quality is, therefore, in the order of 0.075-0.1x10(6) cpm/10 million cells. In addition to the WHO [1999] semen analysis, this range is expected to assist andrologists and clinicians in predicting semen quality and fertilization outcome in patients with male factor infertility undergoing ICSI.     

顶体酶的研究现状及临床应用价值

在诊疗男性不育症的临床实践中,我们逐渐认识到传统的精液分析并不能完全反映精子的受精能力,而精子顶体酶活性作为一种反映精子受精能力的重要指标,逐渐引起更多人的重视。本文将从精子顶体酶的生理作用、影响因素、在诊断和治疗中的价值等四个方面,对目前的研究进展及发展前景进行综述。     

Effect of benzene, toluene, xylene on the semen quality and the function of accessory gonad of exposed workers

The effects on semen and the function of accessory gonad of workers after short and long term exposure to benzene, toluene, and xylene were examined. The semen and blood of 24 married workers exposed to benzene, toluene, and xylene were collected. Routine sperm characteristic, acrosin activity, and Lactate dehydrogenase C4 (LDH-C4) relative activity were detected. The results showed that benzene, toluene, and xylene were found in the blood and semen of some ex-workers at workplaces where the air concentration of benzene, toluene, and xylene exceeded the maximum allowable concentration (MAC). No such solvents were detected in the blood and semen of workers of the control group. The sperm vitality and sperm motility decreased in the exposed workers. The mean acrosin activity, gamma-GT activity and LDH-C4 relative activity in the exposed workers were lower, and fructose concentration was higher than those in the control. There were negative correlations between sperm vitality, sperm activity, acrosin activity, or LDH-C4 relative activity and working history. These results suggest that the mixture of these solvents could affect the sperm and the function of accessory gonad. This might be one reason of the abnormal pregnancy outcome among the wives of workers exposed to benzene, toluene, and xylene.     

Relationship Between Acrosome Reaction and in Vitro Fertilization of Zona-Free Hamster Eggs by Bonnet Monkey (Macaca Radiata) Spermatozoa

The relationship between acrosome reaction, as studied by FITC-RCA staining technique, and the penetration of bonnet monkey spermatozoa into zona-free hamster eggs was investigated. The acrosomes of unreacted spermatozoa fluoresced, whereas those of acro-some-reacted sperm did not fluoresce owing to decreased binding of the lectin. The percentage of acrosome-reacted sperm increased following 3 h of incubation in BWW medium. The assessment of acrosome reaction by the FITC-RCA staining technique correlates well with the in vitro fertilization of zona-free hamster eggs.     

顶体酶的研究现状及临床应用价值

在诊疗男性不育症的临床实践中,我们逐渐认识到传统的精液分析并不能完全反映精子的受精能力,而精子顶体酶活性作为一种反映精子受精能力的重要指标,逐渐引起更多人的重视。本文将从精子顶体酶的生理作用、影响因素、在诊断和治疗中的价值等四个方面,对目前的研究进展及发展前景进行综述。     

DDX25在男性不育患者中的表达及意义

目的探讨DDX25蛋白在男性不育患者中的表达及其相关的临床生物学意义。方法选取2014年6月至2015年12月于成都市妇女儿童中心医院不孕症科就诊的62例男性不育症患者及46位正常体检者的精液标本,利用酶联免疫吸附实验检测精液标本中DDX25的表达情况,硫代巴比妥酸比色法检测精液氧化应激(reactive oxygen species,ROS)水平,精子动态图像检测分析系统计数精子存活率。结果不育患者精液标本中DDX25的表达明显低于正常对照组(P0.05),且不育患者精液标本中ROS的表达较对照组明显增加(P0.05),精子存活率较对照组显著下降(P0.05)。结论男性不育患者中DDX25的表达明显降低,其表达水平与精液ROS水平呈负相关,与精子存活率呈正相关,预示DDX25可能在男性不育的发生、发展过程中起重要作用。     

河南省育龄男性精液质量调查分析

目的:了解河南省育龄男性精液质量现状.方法:随机取集2008年7月～2010年7月到郑州大学第三附属医院人类精子库志愿供精人员及河南省、地市妇幼保健院进行婚前体检和孕前体检的4052名正常育龄男性进行问卷调查,并采用计算机辅助精液分析系统进行精液运动指标分析.结果:4052名志愿者的精液量为2.60±1.39ml,精子...