EGF、TGF-β及胰岛素对兔骨骼肌卫星细胞增殖的影响

目的 研究表皮生长因子（ｅｐｉｔｈｅｌｉｕｍ ｇｒｏｗｔｈ ｆａｃｔｏｒ,ＥＧＦ）、转化生长因子β（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒ－β,ＴＧＦ－β）及胰岛素对兔骨骼肌卫星细胞（ｓａｔｅｌｌｉｔｅ ｃｅｌｌ,ＳＣ）增殖的影响。方法 采用ＭＴＴ法测定ＥＧＦ、ＴＧＦ－β和胰岛素对兔骨骼肌ＳＣ增殖的影响。结果 在体外培养过程中,ＥＧＦ和胰岛素促进兔骨骼肌ＳＣ增殖的作用较ＴＧＦ－β明显     

慢性肾功能不全并发唇痈诱发尿毒症死亡1例

慢性肾功能不全并发唇痈诱发尿毒症死亡１例ＡＣＡＳＥＯＦＣＨＲＯＮＩＣＲＥＮＡＬＩＮＳＵＦＦＩＣＩＥＮＣＹＣＯＭＢＩＮＥＤＣＡＲＢＵＮＣＬＥＯＦＬＩＰＣＡＵＳＩＮＧＵＲＥＭＩＡＴＯＤＥＡＴＨ李建民黄海孙桂格作者单位：安徽省淮南市第一人民医院口腔科（２３...     

内皮素-1(ET-1)在口腔粘膜下纤维性变中的表达水平

为探讨ＥＴ－１与口腔粘膜下纤维性变（ＯＳＦ）的关系,应用免疫组化染色和图像分析技术,检测了ＯＳＦ３０例、正常口腔粘膜（ＮＯＲ）１０例组织中ＥＴ－１的定位分布和含量。结果显示：①ＯＳＦ组织中ＥＴ－１有异常表达,其免疫阳性物质主要分布于上皮棘细胞、基底细胞,间质成纤维细胞、血管内皮细胞及平滑肌细胞的胞浆胞膜上;②ＥＴ－１在ＯＳＦ组织表达的阳性率及相对含量均显著高于ＮＯＲ（Ｐ＜０．０１）;③ＯＳＦ各期ＥＴ－１相对含量存在差别,其中早、中期上皮细胞ＥＴ－１相对含量显著高于晚期（Ｐ＜０．０５）。由此提示,ＥＴ－１可能参与了ＯＳＦ的纤维化过程     

60例腭部小涎腺癌临床分析

６０例腭部小涎腺癌临床分析ＣＡＲＣＩＮＯＭＡＯＦＭＩＮＯＲＳＡＬＩＶＡＲＹＧＬＡＮＤＳＩＮＴＨＥＰＡＬＡＴＥ－ＡＣＬＩＮＩＣＡＬＡＮＡＬＹＳＩＳＯＦ６０ＣＡＳＥＳ王志勇叶炳飞徐明耀作者单位：南京市口腔医院颌面外科（２１０００８）小涎腺癌是腭部最常见的...     

转化生长因子β1在口腔粘膜下纤维性变角朊细胞中的表达

为探讨转化生长因子β１在口腔粘膜下纤维性变发病机理中的作用，作者应用原位杂交方法，对２５例口腔粘膜下纤维性变（ｏｒａｌｓｕｂｍｕｃｏｕｓｆｉｂｒｏｓｉｓ，ＯＳＦ）、５例正常口腔粘膜（ＮＯＲ）及１０例口腔扁平苔藓（ｏｒａｌｌｉｃｈｅｎｐｌａｎｕｓ，ＯＬＰ）组织角朊细胞中转化生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ１，ＴＧＦβ１）ｍＲＮＡ进行了检测。结果：１５例（６０％）ＯＳＦ组织角朊细胞中有ＴＧＦβ１ｍＲＮＡ表达，阳性表达主要分布于早、中期ＯＳＦ组织角朊细胞中；５例ＮＯＲ及１０例ＯＬＰ组织角朊细胞表达呈阴性。结果提示：ＯＳＦ组织角朊细胞可合成分泌ＴＧＦβ１，ＴＧＦβ１在ＯＳＦ的发病机理中可能起重要作用，可能作为一中间介质参与了ＯＳＦ的发病过程。     

口腔颌面部血管外皮肉瘤(附6例报告)

口腔颌面部血管外皮肉瘤（附６例报告）ＨＥＭＡＮＧＩＯＰＥＲＩＣＹＴＩＣＳＡＲＣＯＭＡＯＮＯＲＡＬＡＮＤＭＡＸＩＬＬＯＦＡＣＩＡＬＲＥＧＩＯＮ：ＡＲＥＰＯＲＴＯＦ６ＣＡＳＥＳ杜晓军沈言备黄晓峰血管外皮肉瘤ＨｅｍａｎｇｉｏｐｅｒｉｃｙｔｅＳａｒｃｏ－ｍａ...     

口腔颌面部汽枪伤特点及早期处理

口腔颌面部汽枪伤特点及早期处理ＦＥＡＴＵＲＥＳＡＮＤＥＡＲＬＹＭＡＮＡＧＥＭＥＮＴＯＦＡＩＲＰＵＭＰＧＵＮＳＨＯＲＴＳＩＮＯＲＡＬＡＮＤＭＡＸＩＬＬＯＦＡＣＩＡＬＡＲＥＡ刘建华周群谷志远吴求亮作者单位：浙江医科大学附属一院口腔科（３１０００３）我科自...     

先天性唇腭裂伴全内脏转位先心1例

先天性唇腭裂伴全内脏转位先心１例ＣＯＮＧＥＮＩＴＡＬＣＬＥＦＴＬＩＰＡＮＤＰＡＬＡＴＥＡＮＤＩＮＶＥＲＳＩＯＮＯＦＡＬＬＶＩＳＣＥＲＡ：ＲＥＰＯＲＴＯＦＡＣＡＳＥ周永海作者单位：龙泉市人民医院口腔科（３２３７００）先天性唇腭裂常伴有全身其他脏器的畸形...     

巨型颈动脉体瘤切除后一期人造血管重建

巨型颈动脉体瘤切除后一期人造血管重建ＳＴＡＧＥＩＲＥＣＯＮＳＴＲＵＣＴＩＯＮＶＡＳＣＵＬＡＲＯＰＥＲＡＴＩＯＮＡＦＴＥＲＥＸＣＩＳＩＯＮＯＦＨＵＧＥＣＥＲＶＩＣＡＬＡＮＥＵＲＹＳＭ林朝生储琪东李跃俊余红梅作者单位：上海第二医科大学附属瑞金医院口腔科（...     

BMP和TGFβ对骨生长愈合的协调作用

ＢＭＰ和ＴＧＦβ对骨生长愈合的协调作用ＴＨＥＢＯＮＥＭＯＲＰＨＯＧＥＮＥＴＩＣＰＲＯＴＥＩＮＡＮＤＴＧＦβ司晓辉综述金岩杨连甲审校作者单位：西安第四军医大学口腔医学院病理科（７１００３２）骨组织在整个生命过程中始终经历着新陈代谢，即骨再建过程。骨再建...     

槟榔提取物抑制人类口腔粘膜角朊细胞生长的实验研究

目的：建立人类口腔粘膜上皮角朊细胞体外原代培养方法：探讨口腔粘膜下纤维性变患者上皮厚度改变的机理;方法;先对人类口腔粘膜上皮角朊细胞进行分离,培养和鉴定,然后用四唑盐比色试验检测ＯＳＦ患者和正常人口腔粘膜上皮ＫＣ增殖状况,并且观察槟榔提取物对ＫＣ增殖的影响。     

槟榔提取物刺激口腔黏膜角朊细胞培养上清对成纤维细胞增殖活性的影响

目的：探讨槟榔提取物刺激口腔黏膜角朊细胞在黏膜下纤维性变发病中的作用。方法：采用不同浓度槟榔提取液刺激体外培养的角朊细胞，取细胞培养上清，MTT法观察细胞培养上清对成纤维细胞增殖的影响。结果：一定浓度槟榔提取液刺激的角朊细胞培养上清能促进成纤维细胞增殖；细胞培养上清对成纤维细胞增殖的促进作用存在个体差异。结论：槟榔成分可能通过改变口腔黏膜角朊细胞的活性而导致口腔黏膜下纤维性变的发生。     

槟榔提取物抑制人类口腔黏膜成纤维细胞生长的实验研究

目的通过比较正常口腔黏膜和口腔黏膜下纤维性变(OSF)组织中成纤维细胞(FB)增殖差异、检测槟榔提取物(ANE)对成纤维细胞增殖的影响,来探讨OSF的发病机理.方法对人类口腔黏膜成纤维细胞进行分离培养,然后用四唑盐(MTT)比色试验法检测OSF患者和正常人口腔黏膜FB增殖状况,并且观察ANE对FB增殖的影响.结果表示增殖水平的OD值在OSF-FB为0.254±0.045,高于NM-FB的OD值0.236±0.012(P<0.05),ANE以浓度-效应依赖关系抑制FB增殖.结论 OSF-FB细胞增殖率较NM-FB高;ANE对口腔黏膜FB有细胞毒作用,提示槟榔及其有效成分不完全是通过直接刺激FB增殖而诱发OSF.     

Oral submucous fibrosis: a clinico-histopathological study in Chennai.

BACKGROUND: Oral submucous fibrosis (OSF) is a precancerous condition associated with the use of areca nut in various forms. There are very few reports to correlate the clinical stage to histopathological grading in OSF. MATERIALS AND METHODS: A hospital-based study was conducted on 75 OSF cases who visited our hospital in Chennai from 2000-2003. A detailed history of each patient was recorded along with a clinical examination. Biopsy was performed for histopathological correlation. Clinical stage of the disease in terms of the ability to open one's mouth was correlated with histopathological grading. RESULTS: The male to female ratio of OSF cases was 6:1. All forms of areca nut products were associated with OSF. Chewing of paanmasala was associated with early presentation of OSF as compared to chewing of the betel nut. Out of 57 cases, which were in clinical stage II, 91.2% had histological grading of I and II in equal proportions and 8.8% had histological grade III. Out of 13 cases that showed a clinical stage of III, 52% showed a histological grade of II, 40% grade III and 8% grade I. CONCLUSION: In the present study, there was no direct correlation between clinical stages and histopathological grading. The possibility of difference in the severity and extent of fibrosis in different regions of the oral mucosa and involved muscles were considered as contributory factors for this variation.     

Oral submucous fibrosis: a case-control study in Chennai, South India

BACKGROUND: Oral submucous fibrosis (OSF) is a pre-cancerous condition caused by the use of areca nut in various forms. There are very few published reports on areca nut use and OSF from Chennai, South India. METHODS: A hospital-based case-control study on habits and OSF was performed in Chennai over a 3-year period. A total of 185 consecutive patients with OSF were matched with age- and sex-matched controls. History was recorded in a pre-determined format by qualified dental surgeons. RESULTS: The male to female ratio of OSF cases was 9.9 : 1. All areca nut products were associated with OSF, with the risk being greatest for pan masala. The duration of the habit was more significant than the frequency of the chewing habit. CONCLUSION: The present study confirms the strong association between areca nut use and OSF and the increasing use of pan masala.     

The fibroblast population in oral submucous fibrosis

The purpose of the investigation was to compare the morphology of fibroblasts cultured from healthy oral mucosa and mucosa of patients with oral submucous fibrosis (OSF) and to collate the occurrence of cell types of similar morphology. Cells cultured from biopsy specimens from the buccal mucosa of six subjects who did not chew the areca nut and six patients with OSF who chewed areca nut were grown according to standard techniques. Ninety cells per cell line were recorded daily for 8 days, classified into types F1, F2 and F3 according to their morphology, and the results statistically analyzed. We found that there was a relative increase of F3 cells in relation to Fl cells in OSF resulting in the ratio of F3 to F1 cells being significantly larger in OSF than the ratio in the controls. As it has been reported that F3 cells m rat connective tissues produce significantly more collagen types I and III than F1 cells, we concluded that a change of fibroblast population has occurred in OSF and that this relative increase of F3 cells in humans, which could be committed to the production of large quantities of collagen, can be an explanation for the excessive collagen formation in OSF.     

口腔粘膜下纤维性变临床分析

目的：总结口腔粘膜下纤维性变(OSF)临床特征，提高OSF临床检出率和诊断率。方法：对382例OSF患者临床资料进行总结，对其发病因素，临床特征进行分析。结果：1)382例OSF患者中男性370例(96．8％)，20～40岁以上的OSF患者328例(85％)；2)382例OSF患者均有咀嚼槟榔史，其发病与咀嚼槟榔的时间呈正相关关系，但与咀嚼槟榔的量无正相关关系；3)因热、辣食物有烧灼感、张口困难而就诊者分别为99．2％和50．2％，糜烂或起疱、张口受限者分别为85．8％和50．3％，颊、翼颌韧带受累者分别为96．3％和99．2％，患者常有多个部位受累。结论：1)OSF好发于青年男性；2)槟榔是OSF发病的关键因素；3)OSF临床症状和体征多样。     

Development of an in vivo mouse model to study oral submucous fibrosis

BACKGROUND: Epidemiological data have shown an association of areca nut chewing with oral submucous fibrosis (OSF). Experimental evidence to confirm this has been limited. Fibrosis-promoting activity of areca nut was tested in an animal model. METHOD: Buccal mucosa of a group of 20 female BALB/c strain mice, 10-12 weeks of age, was treated twice daily 6 days per week with topical application of aqueous areca nut extracts for 300-600 days. A control group (n = 20) was treated with 50 mM NaCl. The influence of areca nut on the oral epithelium and connective tissue was recorded semiquantitatively by light microscopy. RESULTS: The areca nut-treated oral epithelium showed progressive changes in epithelial thickness leading to atrophy, increased cellularity of fibroblasts, fibrosis of connective tissue, focal infiltration of inflammatory cells and muscle atrophy. On killing after 600 days of treatment, the scores on cellularity, inflammation and muscle atrophy were significantly different to the control group (P = 0.03). CONCLUSION: The study provides further evidence that areca nut contributes to the development of OSF in treated animals. The model has the potential to test synergism of areca nut with other carcinogens and any therapeutic interventions.