当归补血汤治疗家兔血吸虫病肝纤维化的组织病理学分析

Objective To evaluate the treatment effects on schistosomiasis hepatic fibrosis with Dangguibuxue decoction.Methods Thirty-four rabbits weighing 2.0-2.5 kg were selected.The procedure for the model of schistosomiasis liver fibrosis was as follow.Thirty-four snails infected by schistosoma japonicum were placed in triangle flask to escape cercariain.Abdominal skin of each rabbit wag infected by 100 schistosoma japonicum cercariaes.All rabbits were housed in conventional cages at room temperature.After 13 weeks schistosoma japonicum infected,the rabbits'livers were examined by B-ultrasound examination every week.After 18 weeks of infection,B-ultrasound showed images of schistosomiasis liver fibrosis,the rabbits were thus identified as schistosomiasis liver fibrosis.Praziquantel Was as insecticide to treat rabbits.All rabbits were divided into treatment and control groups according to body weigh.The high and low dosage group were treated with Dangguibuxue decoction at 50 g/kg and 5 g/kg(n=11),respectively,and the control group with no treatment(n=12).Two groups were treated with Dangguibuxue decoction every day for 10 weeks.After 10 weeks of treatment,the rabbits were killed and the livers were obtained to go HE staining and histopathological examination under microscope.Results After 18 weeks of infection,B-ultrasound showed that the liver parenchyma Was coarse and net-shaped.having hyperechoie spots with lightbrand and facula.The liver's shape Was abnormal,portal and splenic vein diameter was widened.Histopathological examination showed that the high dosage group presented fibrosis,which was formed by collagen fiber extending to peripherM regions,low dosage group showed mild fibrosis,mild collagen fiber present with extension without compartment formation, while the control group presented severe fibrosis and severe collagen fiber present with a thickening of the partial compartments and the distribution of eggs nodules. The liver histopathological ranking results from different groups showed that the difference was significant (χ2= 13.585,P < 0.01), the difference between the treatment groups of high and low dosage and control group was significant,respectively(all P < 0.01 ). Conclusions The histopathology results showed that both high and low dosage Dangguibuxue decoction can reverse or eliminate schistosomiasis liver fibrosis in rabbits.     

Experimental therapy of hepatic fibrosis in schistosomiasis japonica. Ⅱ. Effect of colchicine on hepatic fibrosis.

9 rabbits were infected with 80 cercariae of Schistosoma japonicum. 4 months later,development of pipe-stem fibrosis resembling late human schistosomiasis was observed.Colchicine was then used to treat schistosomal hepatic fibrosis at the dosage of 1 mg     

落新妇甙对热缺血再灌注损伤肝脏肿瘤坏死因子α与白细胞介素-10表达的影响

Objective To investigate the effects of astilbin on the expressions of TNF α and IL-10 during liver warm ischemia-reperfusion injury. Methods C57BL/ 6 mice were randomly divided into 4 groups (n = 8): sham-operated group (Sham), model control group(I/R), low dosage of astilbin treatment group (10 mg/kg) and high dosage of astilbin (40 mg/kg) treatment group. The treatment group mice were intraperitoneally injected with 10 or 40 mg/kg astilbin 24 hours and one hour before Ischemia, the hepatic ischemia-reperfusion model were thus established. After jn90 of min ischemia and 6 h reperfusion of the partial hepatic lobe, the expressions of TNF α and IL-10 in liver tissues collected from the experimental groups were detected by Western blot and semiquantitative RT-PCR. Results The expression of TNF a protein in liver tissues gradually decreased in treatment groups (low and high dosages of astilbin treatment groups) as compared to the I/R model control group. Similar results were observed in the mRNA expressions of these genes as determined by semiquantitative RT-PCR (P < 0.05 for low dosage group; P < 0.01 for high dosage group). Compared with the I/R model control group, the expression of IL-10 was increased in both treatment groups (low dosage group P < 0.05; large dosage group P < 0.01). Conclusion Treatment with astilbin decreases TNF α expression but induces IL-10 expression in liver during warm ischemia-reperfusion injury.     

落新妇甙对热缺血再灌注损伤肝脏肿瘤坏死因子α与白细胞介素-10表达的影响

Objective To investigate the effects of astilbin on the expressions of TNF α and IL-10 during liver warm ischemia-reperfusion injury. Methods C57BL/ 6 mice were randomly divided into 4 groups (n = 8): sham-operated group (Sham), model control group(I/R), low dosage of astilbin treatment group (10 mg/kg) and high dosage of astilbin (40 mg/kg) treatment group. The treatment group mice were intraperitoneally injected with 10 or 40 mg/kg astilbin 24 hours and one hour before Ischemia, the hepatic ischemia-reperfusion model were thus established. After jn90 of min ischemia and 6 h reperfusion of the partial hepatic lobe, the expressions of TNF α and IL-10 in liver tissues collected from the experimental groups were detected by Western blot and semiquantitative RT-PCR. Results The expression of TNF a protein in liver tissues gradually decreased in treatment groups (low and high dosages of astilbin treatment groups) as compared to the I/R model control group. Similar results were observed in the mRNA expressions of these genes as determined by semiquantitative RT-PCR (P < 0.05 for low dosage group; P < 0.01 for high dosage group). Compared with the I/R model control group, the expression of IL-10 was increased in both treatment groups (low dosage group P < 0.05; large dosage group P < 0.01). Conclusion Treatment with astilbin decreases TNF α expression but induces IL-10 expression in liver during warm ischemia-reperfusion injury.     

Effect of Rougan Huaqian granules combined with human mesenchymal stem cell transplantation on liver fibrosis in cirrhosis rats

Objective:To observe the effect of Rougan Huaqian granules combined with human mesenchymal stem cell(hMSC)transplantation on the liver fibrosis in carbon tetrachlorideinduced cirrhosis rats.Methods:Sixty SD rats were randomly divided into live groups.The rats in control group received intraperitoneal injection of saline,while those in model control group,treatment group A,group B and group C received intraperitoneal injection of carbon tetrachloride oily solution to induce liver cirrhosis within 8 weeks.Then,the rats in the model control group,treatment group A,treatment group B,treatment group C received vein tail injection of saline,Rougan Huaqian granules,hMSC suspension and Rougan Huaqian granules combined with hMSC suspension.Results:The treatment groups had significantly different liver function(AST levels),liver fibrosis index(laminin and HA),hepatic sinusoidal wallsα-smooth muscle actin,Ⅳcollagen and laminin protein expression andⅠ,Ⅲcollagen from the model group(P0.05).The transplanted cells showed human hepatocyte-like cells differentiation trend in the liver.Conclusions:The Rougan Huaqian granules combined with hMSC transplantation can alleviate liver fibrosis in cirrhosis rats.     

Effect of Sinai san decoction on the development of non-alcoholic steatohepatitis in rats

AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats. METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n=9), model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, and those of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks. RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group. CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet, but does not prevent the development of hepatocyte steatosis.     

Effect of matrine on transforming growth factor β1 and hepatocyte growth factor in rat liver fibrosis model

Objective:To observe the preventive and control effect of matrine on transforming growth factor(TCF- β1) and hepatocyte.growth factor(HCF) of liver fibrosis tissue in rals.Methods:A total of48 SD rats were randomly divided into A,B,C,D groups with 12 in each,group A as the normal control group and groups B.C,D as liver fibrosis models using composite modulus method with carbon tetrachloride(CCL_4).Group B was the model group,group C adopted γ— interferon lavage therapy in the second day of modeling,and group D adopted matrine lavage treatment,at 4 and8 weeks after treatment.Six rats were executed for detection of TGF- β1 and HGF,liver tissue histology and comparison fibrosis degree changes of rat liver tissue between groups.Results:Croups B,C,D showed a more significantly increased TCF- β1 at each time point compared with group A(P0.05);Group B showed a more significantly increased TGF- β1 than groups C and D at weeks 4 and 8(P0.05);group D showed a lowest level of TGF-β1,followed by groups C and B.HGF of group B decreased more significantly than A group at weeks 4 and 8(P0.05);HGF of groups C and D was significantly elevated at 4 and 8 weeks than groups A and B(P0.05),in which the group D showed the highest level of HGF.According to tissue histologic observation,rat liver tissue structure of group A was clear and normal,tissue structure of group B was destroyed with obvious fibrous tissue hyperplasia and fatty change of hepatic cells;groups C and D showed a slighter liver tissue damage,cell necrosis and connective tissue hyperplasia in collect abbacy than group B with a trend of obvious improvement.Conclusions:Matrine can reduce TGF- β1expression and enhance the activity of HGF,so as to realize the inhibition effect on liver fibrosis in rats.     

Inhibitory effect of HuangqiZhechong decoction on liver fibrosis in rat

AIM:To assess the inhibitory effect of Huangqi Zhechongdecoction on hepatic fibrosis in rats induced by CCl_4 plusalcohol and high fat low protein diet.METHODS:Male SD rats were randomly divided into hepaticfibrosis model group,control group and 3 treatment groupsconsisting of 12 rats in each group.Except for the normalcontrol group,all the rats were subcutaneously injected withCCl_4 at a dosage of 3 mL/kg.In 3 treated groups,eitherhigh-dose group(9 mL/kg),or medium-dose group(6 mL/kg),or low-dose group(3 mL/kg)was daily garaged with HuangqiZhechong decoction,and saline vehicle was given to modeland normal control rats.Enzyme-linked immunosorbent assay(ELISA)and biochemical examinations were used todetermine the changes of alanine aminotransferase(ALT),aspartate aminotransferase(AST),hyaluronic acid(HA),laminin(LN),type-Ⅲ-procollagen-N-peptide(PIIIP),andtype Ⅳ collagen content in serum,and hydroxyproline(Hyp)content in liver after sacrificing the rats.Pathologic changes,particularly fibrosis were examined by hematoxylin and eosin(HE)and Van Gieson staining.RESULTS:Compared with the model control group;serumALT,AST,HA,LN,PⅢP and type Ⅳ collagen levels droppedmarkedly in Huangqi Zhechong decoction groups,especiallyin the medium-dose Huangqi Zhechong decoction group(1954 576 U/L vs 759±380 U/L,2 735±786 U/L vs1259±829 U/L,42.74±7.04 ng/mL vs 20.68±5.85 ng/mL,31.62±5.84 ng/mL vs 14.87±1.45 ng/mL,3.26±0.69 ng/mLvs 1.47±0.46 ng/mL,77.68±20.23 ng/mL vs 25.64±4.68ng/mL,respectively)(P<0.05).The Hyp content in liver tissuewas also markedly decreased(26.47±11.24 mg/mgprot vs9.89±3.74 mg/mgprot)(P<0.01).Moreover,the stage ofthe rat liver fibrosis in Huangqi Zhechong decoction groupswas lower than that in model group,and more dramaticdrop was observed in medium-dose Huangqi Zhechongdecoction group(P<0.01).CONCLUSION:Huangqi Zhechong decoction can inhibithepatic fibrosis resulted from chronic liver injure,retard thedevelopment of cirrhosis,and notably ameliorate the liver function.It may be a safe and effective therapeutic drug forpatients with fibrosis.     

The utility of two-dimensional shear wave elastography and texture analysis for monitoring liver fibrosis in rat model

Background:Liver fibrosis is a common pathological change caused by a variety of etiologies.Early diagnosis and timely treatment can reverse or delay disease progression and improve the prognosis.This study aimed to assess the potential utility of two-dimensional shear wave elastography and texture analysis in dynamic monitoring of the progression of liver fibrosis in rat model.Methods:Twenty rats were divided into control group(n = 4) and experimental groups(n = 4 per group) with carbon tetrachloride administration for 2,3,4,and 6 weeks.The liver stiffness measurement was performed by two-dimensional shear wave elastography,while the optimal texture analysis subsets to distinguish fibrosis stage were generated by Ma Zda.The results of elastography and texture analysis were validated through comparing with histopathology.Results:Liver stiffness measurement was 6.09 ± 0.31 k Pa in the control group and 7.10 ± 0.41 k Pa,7.80 ± 0.93 k Pa,8.64 ± 0.93 k Pa,9.91 ± 1.13 k Pa in the carbon tetrachloride induced groups for 2,3,4,6 weeks,respectively(P 0.05).By texture analysis,histogram and co-occurrence matrix had the most frequency texture parameters in staging liver fibrosis.Receiver operating characteristic curve of liver elasticity showed that the sensitivity and specificity were 95.0% and 92.5% to discriminate liver fibrosis and non-fibrosis,respectively.In texture analysis,five optimal parameters were selected to classify liver fibrosis and non-fibrosis.Conclusions:Two-dimensional shear wave elastography showed potential applications for noninvasive monitoring of the progression of hepatic fibrosis,even in mild fibrosis.Texture analysis can further extract and quantify the texture features in ultrasonic image,which was a supplementary to further visual information and acquired high diagnostic accuracy for severe fibrosis.