聚合酶链技术在慢性前列腺炎诊治中的应用

目的：探索慢性前列腺炎的病因，提高其诊治水平。方法：应用聚合酶链技术(PCR)对门诊疑诊为慢性前列腺炎患者的前列腺液进行淋球菌(NG)、沙眼衣原体(CT)及解脲支原体(UU)检测。结果：检出单纯淋球菌感染33例，阳性率9．09％；沙眼衣原体感染32例，阳性率5．15％；解脲支原体感染250例，阳性率23．00％，沙眼衣原体与解脲支原体合并感染13例；淋球菌合并解脲支原体感染5例；淋球菌合并沙眼衣原体感染4例；并应用PCR监测治疗效果。结论：PCR检测慢性前列腺炎病原体快速、敏感性高、特异性强，是一种较为理想、值得推广的临床应用技术。     

136例正常男性尿道内沙眼衣原体和支原体检测

非淋菌性尿道炎(nongonococcalurethritis,NGU)是指由淋球菌以外其它病原体所引起尿道炎。引起非淋菌尿道炎的常见病原体是沙眼衣原体和解脲脲原体[1]。因此,尿道分泌物的病原学检测时有重要的诊断辅助价值。但是正常男性尿道是否存在沙眼衣原体和解脲脲原体呢?为了解正常男性尿道内沙眼衣原体和解脲脲原体存在情况,我们对136例正常男性尿道进行了沙眼衣原体和支原体检测研究。材料和方法1.病例选择:136例志愿者均系20~40岁健康男性;所有被检测者均在检测后随访30d,确认无潜伏期患者存在。2.试剂:沙眼衣原体的检测应用AppliedBiotech公司…     

荧光探针定量聚合酶链反应检测684例女童泌尿生殖道淋球菌、沙眼衣原体、解脲支原体感染情况

目的了解女童泌尿生殖道淋病双球菌、沙眼衣原体、解脲支原体感染情况。方法对684例有泌尿生殖道感染症状的女童取阴道分泌物,采用荧光探针定量聚合酶链反应（PCR-荧光探针法）进行淋病双球菌、沙眼衣原体、解脲支原体检测。结果684例患儿19例淋病双球菌阳性,12例沙眼衣原体阳性,31例解脲支原体阳性。结论对于有反复泌尿生殖道感染的女童,临床上应考虑相关病原体感染的可能,建议进行此3种病原体的检查,以指导临床治疗。     

性传播性尿道炎后慢性前列腺炎(附86例报告)

目的　探讨性传播性尿道炎后慢性前列腺炎患者的病因学特点。　方法　对 86例性传播性尿道炎后慢性前列腺炎患者进行前列腺按摩液 (EPS)的病原体检测并分析结果。　结果　EPS细菌培养阳性 5 7例 ,阳性率 6 6 .3% ,其中以淋球菌为主要致病菌 33例 (38.4 % )。前列腺液PCR检测淋球菌阳性 37例 (43.0 % ) ,沙眼衣原体阳性 2 2例 (2 5 .6 % ) ,解脲脲原体阳性 19例(2 2 .0 % )。PCR检测出复合病原体者 12例 (13.9% )。　结论　性传播性尿道炎后慢性前列腺炎的致病菌以淋球菌、衣原体和解脲脲原体为主 ,治疗前行EPS病原学检测有重要意义     

延安地区泌尿生殖道淋球菌和非淋菌感染情况分析

<正>近10年监测资料显示,我国性病流行呈持续增长趋势,而淋病(gonorrhea,GU)和非淋菌性尿道炎(nongonococcal urethritis,NGU)位列我国性病中发病数前列[1]。淋球菌(Neisseria gonorrhoeae,NG)、沙眼衣原体(Chlamydozoa trachomatis,CT)和解脲脲原体(Ureaplasma urealyticum,UU)是引起以上性病的主     

慢性前列腺炎病原学的初步研究

目的对慢性前列腺炎的尸体前列腺组织标本进行病原学、病理组织学变化的研究,并对病原体与病理组织学变化的相关性进行初步探讨。方法从１９９６年５月～１９９７年１２月,对１１４例２０～４０岁慢性前列腺炎组织标本用细菌学培养、多聚酶链反应（ＰＣＲ）,对组织内的沙眼衣原体、解脲脲原体、病毒、淋球菌进行检测及病理组织学检查。结果１１４例前列腺组织标本中病原体检测阳性５９例（５２％）,其中单纯细菌性病原体阳性４２例,单纯沙眼衣原体（ＣＴ）６例,人类巨细胞病毒（ＣＭＶ）２例,解脲脲原体（ＵＵ）１例,人体乳头状病毒（ＨＰＶ）１例,７例为复合病原体。所有标本均存在不同程度的灶性慢性前列腺炎病理组织学变化。临床检测的病原体并不引起特异性病理组织学变化。结论慢性前列腺炎有单纯的病原体感染,还有复合感染,应予重视。慢性前列腺炎发病机制多为逆行感染,其病理组织学变化可能与检测到的病原体无关     

泌尿系统解脲脲原体感染耐药性分析

目的 探讨成年患者泌尿系统解脲脲原体感染状况及抗菌药物的耐药情况，为临床治疗解脲脲原体感染提供依据。 方法 回顾性分析大连医科大学附属第一医院2012年1月1日—2013年12月31日送检的尿液标本2 231份，采用培养鉴定、半定量计数和药敏一体化的 方法 进行支原体培养，检测解脲脲原体对9种抗菌药物的耐药情况。结果 2 231份送检标本中单纯解脲脲原体感染628株，检出率28.1%，药敏结果 显示喹诺酮类耐药率为79.5%，大环内酯类耐药率为23.2%，四环素类耐药率为0.6%。强力霉素、米诺环素对解脲脲原体的药物敏感性平均高达98.2%。大环内酯类药物克拉霉素和交沙霉素对解脲脲原体敏感性较高，分别为85.4%，62.3%，阿奇霉素和罗红霉素的敏感性较低，分别为34.1%，20.7%。结论 成年患者泌尿系统支原体感染较为常见，解脲脲原体为其主要病原微生物，喹诺酮类药物耐药率最高，四环素类药物耐药率最低，四环素类药物可能是临床上治疗解脲脲原体感染的有效药物。     

PCR荧光探针法、乳胶法及培养法检测生殖道分泌物中沙眼衣原体、淋球菌和解脲支原体的优劣分析

目的比较PCR荧光探针法、乳胶法及培养法对生殖道分泌物中沙眼衣原体、淋球菌和解脲支原体的检出率差异,评价不同检测方法对不同性病病原体的检测意义。方法收集性病门诊患者的泌尿生殖道分泌物,采用PCR荧光探针法检测沙眼衣原体、淋球菌和解脲支原体,同时采用乳胶法检测沙眼衣原体,培养法检测淋球菌和解脲支原体,并将PCR荧光探针法的检测结果与乳胶法和培养法进行比较。结果 587例标本同时完成沙眼衣原体PCR荧光探针法和乳胶法检测,PCR荧光探针法检测阳性率为11.9%(70/587)显著高于乳胶法(2.9%,17/587),差异具有统计学意义(P0.001);609例标本同时完成淋球菌PCR荧光探针法和培养法检测,PCR荧光探针法检测阳性率为9.0%(55/609)略高于培养法(7.4%,45/609),差异无统计学意义(P0.05);1022例标本同时完成解脲支原体PCR荧光探针法和培养法检测,PCR荧光探针法检测阳性率为44.9%(459/1022)高于培养法(40.0%, 409/1022),差异具有统计学意义(P0.05)。结论与乳胶法相比,PCR荧光探针法能显著提高沙眼衣原体的阳性检出率,有助于临床的早期诊断和及时治疗;相较于培养法,PCR荧光探针法能够提高解脲支原体的阳性检出率,对淋球菌的检出率与培养法相近。PCR探针法具有特异性高、敏感性强、操作简便等优点,培养法检测可以进行病原菌耐药性分析,指导临床合理用药,对于考虑淋球菌或解脲支原体感染的患者,可选择PCR探针法和培养法同时检测。     

男性患者泌尿生殖道感染病原体荧光定量PCR检测分析

正近年来我国性传播疾病(STD)的发病率呈逐年上升趋势,淋球菌(Neisseria gonorrhoeae,NG)、沙眼衣原体(Chlamydozoa trachomatis,CT)、解脲支原体(Ureaplasma urealyticum,UU)是常见的性传播疾病的病原体,其不仅可以引起男性生殖道炎症,还可以导致精子液化时间延长,精子浓度、活力、活率降低,畸形率增高,导致男性不育,严重影响男性健康[1-2]。为了解本地区男性泌尿道感染患者淋球菌、沙眼衣原体和解脲支原体的感染情况和流行病学特点,笔者对本院皮肤性病科门诊就诊的920例男性疑似泌尿生殖道感染患者的病原相关检测结果进行了统计分析,现将结果报告如下。     

尿道张力性药物灌注治疗非淋病性尿道炎

尿道张力性药物灌注治疗非淋病性尿道炎吴怡兴非淋病性尿道炎（ＮＧＵ）大多由沙眼衣原体（约５０％）及解脲支原体（约３５％）感染所致。近年来，临床上遇到经久不愈的顽固病例有逐渐增多之势。我院应用聚合酶链反应（ＰＣＲ）技术确诊并施行尿道张力性保留药物灌注治疗...     

Etiology, manifestations and therapy of acute epididymitis: prospective study of 50 cases.

There were 50 patients with acute epididymitis who were evaluated prospectively by history, examination and microbiologic studies, including cultures for aerobes, anaerobes, Neisseria gonorrhoeae, Chlamydia trachomatis and Ureaplasma urealyticum. Escherichia coli was the predominant pathogen isolated from the urine of men more than 35 years old, while Chlamydia trachomatis and Neisseria gonorrhoeae were the predominant pathogens isolated from the urethra of men less than 35 years old. The etiologic role of Escherichia coli and Chlamydia trachomatis was confirmed by isolation from epididymal aspirates from a high proportion of men with positive urine or urethral cultures for these agents. Chlamydia trachomatis epididymitis accounted for two-thirds of idiopathic epididymitis in young men and often was associated with oligospermia. Of 9 female sexual partners of men with Chlamydia trachomatis infection 6 had antibody to Chlamydia trachomatis, of whom 2 had positive cervical cultures for this organism and 2 others had non-gonococcal pelvic inflammatory disease. Antibiotic therapy with tetracycline was effective for the treatment of men with Chlamydia trachomatis epididymitis and should be offered to the female sex partners.     

用DNA探针检测沙眼衣原体

目的 :建立一种敏感而特异的沙眼衣原体分子生物学检测方法。　方法 :用PCR扩增 5 17bp的沙眼衣原体种特异性基因片段并标记成探针 ,建立DNA探针杂交检测沙眼衣原体的方法。　结果 :探针只与沙眼衣原体L2、TE5 5株DNA呈阳性杂交斑点 ,与其他两种衣原体、解脲支原体、淋病奈瑟菌、大肠埃希菌、金黄色葡萄球菌、流感嗜血杆菌及白色念珠菌DNA斑点膜无阳性杂交信号。从 10 0例慢性宫颈炎和前列腺炎病人生殖道分泌物中检出阳性 2 2例 ,阳性率 2 2 %。　结论 :建立的DNA探针检测沙眼衣原体方法具有较高的敏感性和特异性 ,可用于批量临床标本的检测     

The prevalence of potential pathogenic micro-organisms in the endocervix of pregnant women at Tygerberg Hospital.

The prevalence of potential pathogenic organisms cultured from the endocervix in 206 pregnant patients was: Ureaplasma urealyticum 63%, Mycoplasma hominis 39%, Candida albicans 20%, Chlamydia trachomatis 11%, Neisseria gonorrhoeae 7%, group B beta-haemolytic streptococcus 6% and Listeria monocytogenes 1%. The most common complications of pregnancy found were: preterm labour 14%, urinary tract infection 8% and premature rupture of membranes 4%. Significantly more patients in whom C. trachomatis or C. albicans were found delivered prematurely. Other organisms were not associated with preterm labour.     

中性粒细胞弹性蛋白酶在男性生殖道感染诊断中的意义

中性粒细胞弹性蛋白酶 (NE)是一种中性蛋白酶。近年来 ,它在诊断男性生殖道感染中的作用日益受到人们的重视。它不仅对于筛选由奈瑟双球菌、沙眼衣原体、解脲支原体、毛滴虫等感染有特异的诊断价值 ,对白细胞精子症、睾丸鞘膜炎等多种疾病也有较高的特异性。NE已被作为诊断男性生殖道感染的特异性标志物     

南京地区高危人群性传播疾病感染情况分析

目的 :了解南京地区高危人群中性传播疾病 (STD)的流行状况。　方法 :对南京市 15 39例高危人群进行体检及淋病奈瑟菌 (GN)、梅毒螺旋体 (SY)、尖锐湿疣 (CA)、沙眼衣原体 (Ct)、溶脲脲原体 (Uu)以及人类免疫缺陷病毒(HIV)抗体实验室检查。　结果 :GN、SY、CA、Ct、Uu的感染率分别为 13.5 %、10 .3%、2 .3%、35 .9%、2 2 .4 % ,发现 0例HIV抗体阳性者。非淋菌性尿道炎是感染率最高的STD。　结论 :加强对高危人群STD的筛查是防治STD传播的重点。     

The importance and frequency of mixed infections with Chlamydia trachomatis and mycoplasmas in acute gonococcal urethritis

Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis are frequently involved in gonococcal urethritis. We investigated 143 male White and Black patients with gonococcal urethritis (average age 22,5 years). Of these 29% had coexisting Chlam. trachomatis infection, 34% U. urealyticum infection and 13% Mycoplasma hominis infection. Conventional penicillin therapy did not affect Chlam. trachomatis, U. urealyticum or Mycoplasma hominis, which persisted in the lower urogenital tract, causing a so-called 'post-gonococcal urethritis.' Additional therapy with tetracycline or erythromycin was successful in most cases.     

The frequency of Chlamydia trachomatis in acute epididymitis

Twenty-five patients with bacteriologically proven epididymitis were studied between 1984 and 1986. Thirteen were less than 35 years old and in 11 of them Chlamydia trachomatis was isolated; of the 2 remaining patients Escherichia coli was found in 1 and Neisseria gonorrhoeae in the other. In patients more than 35 years old, gram negative bacteria were the cause of infection in 10 and only 1 case was attributable to Chlamydia trachomatis. These results suggest that Chlamydia trachomatis is much more frequent in men under the age of 35 and the difference is statistically significant.     

Efficacy of various single-dose regimens of ceftriaxone in uncomplicated acute gonococcal urethritis in adult males

The therapeutic efficacy of single intramuscular doses of ceftriaxone (Rocephin; Roche) (62.5, 125 and 250 mg), administered without probenecid, was evaluated in 167 adult males with uncomplicated acute gonococcal urethritis. Cure rates of 100% were achieved at 62.5 mg and 250 mg. In the 125 mg dose group, Neisseria gonorrhoeae was isolated from 1 patient at follow-up after therapy. Reinfection was suspected, since this patient returned on day 10 and admitted to sexual contact 2 days previously. Side-effects were minimal, and patient acceptance was better for ceftriaxone dissolved in lignocaine than in sterile water. Chlamydia trachomatis was detected at follow-up in 14.4% patients, confirming that ceftriaxone has no significant effect on chlamydial infection and additional treatment is necessary for patients with coexistent infection.     

Acute epididymitis: etiology and therapy.

Fifty patients with acute epididymitis were evaluated prospectively by history, examination, and microbiologic studies, including cultures for aerobes, anaerobes, N. gonorrhoeae, Chlamydia trachomatis, and Ureaplasma urealyticum. E. Coli was the predominant pathogen isolated from the urine of men over 35 years old and C. trachomatis and N. gonnorrheae were the predominant pathogens isolated from the urethra of men under 35. The etiologic role of E. Coli and C. trachomatis was confirmed by isolation from epididymal aspirates from a high proportion of men with positive urine of urethral cultures for these agents. C. trachomatis epididymitis accounted for two thirds of "idiopathic epididymitis" in men, and was often associated with oligozoospermia. Of nine female sexual partners of men with C. trachomatis infection, six had antibody to C. trachomatis, of whom two had positive cervical cultures for this organism, and the others had nongonococcal pelvic inflammatory disease. Antibiotic therapy with tetracycline was effective for the treatment of men with C. trachomatis epididymitis, and should be offered to their female sex partner.