妊娠妇女血清铜、铁、锌含量的调查

目的 探讨妊娠妇女血清中铜、铁、锌含量变化及临床意义.方法用新天WFX-Ⅱ型原子吸收分光光度计分别测70名妊娠妇女和45名非妊娠妇女血清中的铜、铁、锌含量.结果妊娠妇女血清铜高于非妊娠妇女(P＜0.001),而妊娠妇女血清锌低于非妊娠妇女(P＜0.001),Cu/Zn比值妊娠妇女高于非妊娠者,血清铁及Fe/Cu比值,两者比较差异无显著意义.结论妊娠妇女血清铜增高,且Cu/Zn比值也增高,提示妊娠妇女可能存在缺铁性贫血.另外妊娠妇女血清锌低于正常,说明妊娠妇女应补充锌剂.     

微量元素铜,锌与妇科肿瘤

采用原子吸收分光光度计测量147例妇科良、恶性肿瘤的血清铜、锌和血清铜/锌比值,并对4例恶性滋养细胞肿瘤进行治疗前、后的动态观察。结果恶性肿瘤患者血清铜含量比良性肿瘤明显升高,而锌含量比良性肿瘤明显低;恶性肿瘤血清铜/锌值均>1.7O,与良性肿瘤相比有高度显著性差异(P<0.01);恶性滋养细胞肿瘤治疗后的血清铜/锌比值有明显下降。认为血清铜、锌和血清铜/锌比值可以作为鉴别妇科良恶性肿瘤的辅助指标之一。     

微量元素与太行山区三县西部山区胃癌发病的相关性研究

目的探讨邢台县、沙河县、临城县三县西部山区村庄胃癌患者体内部分微量元素与胃癌发病的关系。方法采用美国Perkin-Elemer 603 AAS原子吸收分光光度计对60例胃癌患者血清及肿瘤组织中铜、铁、锌、硒、镉5种微量元素的含量进行测定,并以60名健康人血清为对照。结果与对照组相比,胃癌患者血清中铜、镉含量和铜、锌比值明显增高,而锌、硒、铁含量降低,差异有统计学意义(P<0.05);胃癌患者癌组织中铜、镉含量和铜/锌比值明显高于血清,而锌、硒含量则明显低于血清(P<0.05),两者之间差异无统计学意义(P>0.05)。结论微量元素及其比值的改变与太行山区三县农村胃癌的发生存在着密切的关系。     

肺炎患儿血清锌、铜含量和比值的变化及其临床意义

锌和铜是人体必需的微量元素,二者代谢有着密切关系,而在感染时其血清锌、铜含量可发生变化。我们于1988年3～5月对住院的40例肺炎患儿观察了血清锌、铜含量及其比值的变化,并随     

老年呼吸道感染伴C—反应蛋白增高患者血清微量元素的改变

通过４８例老年呼吸道感染患者血清微量元素锌，铜，镁，硒含量，铜／锌比值及Ｃ－反应蛋白（Ｃ－ＲＰ）的测定，观察到老年呼吸道感染患者血清Ｃ－ＲＰ增高与血清微量元素锌，镁，硒，降低，铜升高，铜／锌比值升高等变化同时出现，提示二者都与老年呼吸道感染关系密切。但Ｃ－ＲＰ的升高作为机体对炎症的反应，却与微量元素间并无直接相关关系。     

再生障碍性贫血患者血清微量元素与临床意义探讨

目的：探讨血清铁、铜、锌、铅等微量元素含量与再生障碍性贫血之间的关系,并与外周T淋巴细胞亚群比较,分析其临床意义。方法采用原子吸收分光光度法测定50例再生障碍性贫血患者和40例健康正常人的血清铁、铜、锌、铅含量。流式细胞仪检测患者外周血T淋巴细胞亚群。结果初治再生障碍性贫血患者血清铁、铅、铜含量及铜/锌比值均高于正常对照组（ P ＜0．01）,而锌含量明显低于对照组（ P ＜0．01）。铜/锌比值与网织红细胞及CD4＋/CD8＋负相关（均P ＜0．05）。治疗后患者血清铁、铜、锌、铅及铜/锌比值均不同程度恢复。治疗有效组上述微量元素水平与正常对照组差异无统计学意义（ P ＞0．05）。结论微量元素参与再生障碍性贫血的发病及转归。     

微量元素与卵巢癌的关系及临床应用

采用ＰＥ公司３０３０原子吸收分光光度计，对５４例卵巢癌、２０例卵巢良性疾病患者进行血清、头发、组织中铜、锌、铁、锰、钙水平和铜／锌比值的测定。并以５０例健康成年女性为对照，测定结果进行单因素分析，卵巢癌患者血清、头发和组织中的铜、锰水平及铜／锌比值明显高于良性疾病组和正常对照组，而锌、铁、钙明显低于正常对照组，差异显著，分别（Ｐ＜０．０１～０．００１）。非条件Ｌｏｇｉｓｔｉｃ回归多因素分析表明有：卵巢癌患者血清铜／锌比值的比数比为５９．８０，９５％ＣＩ（１２．７３～２８０．９２）。提示：铜／锌比可能是卵巢癌发病危险因素。结果还表明，以健康对照组铜／锌比值１．１０为界值，对卵巢癌诊断阳性率为８１．４８％。提示铜／锌比值对卵巢癌的诊断，良恶性的鉴别有重要价值。     

精神分裂症患者血清中微量元素铜、铁、锰、锌测定

本文测定93例精神分裂症患者血清铜,铁,锰,锌的含量,并观察其变化与临床表现间关系。精神分裂症患者血清铜的含量(102.38±11.83,±SE)与正常对照组(101.20±13.30)无区别,但血清铁(104.84±25.04),锰(0.41±0.17),锌(83.67±20.03)含量均较对照组(分别为135.83±18.46,0.73±0.09,115.63±8.05)为低。治疗后血清铜含量下降(94.44±13.15),锌水平上升达于正常(117.74±18.01),锰(0.42±0.17)和铁(112.56±33.67)水平仍低。铜/锌比值在治疗前为1.223,治疗后为0.875(正常值为0.82),表明疾病急性期铜/锌比值升高,治愈后恢复正常。这些变化可能与体内或脑内存在着微量元素代谢异常有关。     

糖尿病患者血清八种元素与慢性并发症关系的探讨

对49例糖尿病(NIDDM)患者和100例正常人测定血清锌、铜、锰、镁、镉、铬、钼、钙8种元素。结果可示糖尿病患者血清锌、铬、钼、镁均明显下降,分别为1.01±0.36μg/g、0.25±0.17μg/g、0.24±0.18μg/g、24.32±5.85μg/g,全部P<0.01。锌随肾渗透性利尿及尿蛋白丢失而下降,镁随病程延长而下降。镉明显上升,为0.019±0.024μg/g,P<0.01。铜/锌比值明显升高。低镁、高钙对眼病的发生发展;低铬、低钼对动脉粥样硬化的形成发展;高镉对高血压的形成;肾病者锌与镁的变化均起一定作用。重点讨论糖尿病慢性并发症的元素变化特点。     

微量元素锌与小儿疾病

微量元素锌在小儿生长发育过程中起着重要的作用 ,如果在生长发育中锌元素缺乏 ,就会发生某些疾病。主要有 :(1)锌与胎儿酒精中毒综合征 :在研究中发现 ,酗酒的母亲血浆中锌水平降低 ,慢性酒精中毒可以出现锌缺乏 ,所以其婴儿可出现酒精中毒综合征。 (2 )锌与小儿格林 巴利综合征[1 ] :小儿格林 巴利综合征患者的血清锌 铜比值明显低于正常儿童的锌 铜的比值 ,也低于成人锌 铜的比值 ,说明小儿格林 巴利综合征患者存在血清微量元素锌和铜代谢异常。当小儿机体缺微量元素锌和铜时 ,因体内白细胞内生介质的作用 ,血清锌下降 ,血清铜增高…     

The serum concentrations of zinc, copper and selenium in children with inflammatory bowel disease

OBJECTIVE: To estimate the levels of trace elements in children with inflammatory bowel disease (IBD). DESIGN: Prospective cross sectional study. SETTING: Gastroentrology Unit, Great Ormond Street Children's Hospital, London, UK. SUBJECTS: Seventy four children with inflammatory bowel disease confirmed endoscopically and histologically (38 ulcerative colitis and 36 Crohn's disease) and 40 age matched controls had their serum zinc, copper and selenium assayed at presentation. MAIN OUTCOME MEASURE: Serum levels of zinc, copper and selenium in children with inflammatory bowel disease and age matched controls. RESULTS: Seventy four children with inflammatory bowel disease confirmed endoscopically and histologically (38 ulcerative colitis and 36 Crohn's disease) and 40 age matched controls had their serum zinc, copper and selenium assayed at presentation. The serum levels of selenium were significantly lower in cases of ulcerative colitis 0.63 +/- 0.25 mmol/L and Crohn's disease 0.69 +/- 0.25 mmol/L than in the controls 0.84 +/- 0.13 mmol/L (p < 0.01). The serum copper concentration was significantly higher in those with Crohn's disease 22.7 +/- 5.49 mmol/L than in those with ulcerative colitis 17.6 +/- 5.15 mmol/L and the controls 20.76 +/- 4.06 mmol/L (p < 0.01). Children with Crohn's disease had a lower serum zinc level 11.01 +/- 2.49 mmol/L compared to the control level of 13.6 +/- 1.63 mmol/L (p < 0.05), but the levels were not significantly different in the controls and ulcerative colitis (p > 0.10). Children with inflammatory bowel disease have abnormal levels of the trace elements which is more marked in those with Crohn's disease. CONCLUSION: Children with IBD in this study show abnormalities of the trace elements which is probably a result of inadequate intake, reduced absorption, increased intestinal loss due to impairment of the absorption as a result of the inflammatory process. The reduced free radical scavenging action of zinc and selenium as a result of their deficiency may contribute to the continued inflammatory process of IBD. The recommendation of the supplementation of these trace elements in IBD is further supported by the findings of this study in children.     

葡萄糖酸锌治疗肝豆状核变性31例

20例肝豆状核变性患者口服葡萄糖酸锌1.6g/d,14例症状改善。治疗前后对比,血清铜降低显著(P<0.05),血清锌显著升高(P<0.01),血清Cu/Zn比值下降显著(P<0.01);脑脊液铜降低不显著,脑脊液锌升高较显著(P<0.05),脑脊液Cu/Zn比值降低显著(P<0.05);24h尿排铜显著增高(P<0.01)。11例服0.84 g/d组,8例症状改善;但治疗前后对比血清和脑脊液铜、锌测定及24h尿排铜等均无显著变化。认为口服本药可替代硫酸锌治疗肝豆状核变性。     

Serum zinc and serum copper and indices of drug metabolism in alcoholics

Summary Serum zinc and copper levels were studied in relation to in vitro and in vivo drug metabolism in 25 alcoholics, in whom various diseases of the liver had been diagnosed by histology. Serum zinc was elevated in alcoholics with normal or fatty liver and was low in those with alcoholic hepatitis or cirrhosis. There was a significant positive correlation between serum zinc and cytochrome P-450 content of liver biopsies. The relationship between zinc and antipyrine half-life was significant and non-linear. Serum copper level was elevated in all the alcoholics and no significant relationship could be found between copper and drug metabolism in alcoholics. The findings suggest parallelism between changes in serum zinc and indices of drug metabolism in alcoholics.     

Changes in zinc, copper and selenium status during adjuvant-induced arthritis in rats

Trace elements such as zinc, copper and selenium are involved in the pathogenesis of inflammatory diseases. In order to obtain more information about the overall movements of these minerals during the evolution of an experimental chronic inflammatory process, trace element levels were determined in five body compartments of the rat at several time intervals after induction of adjuvant arthritis. Rapid and significant changes in plasma zinc and copper levels and in liver zinc levels were observed. These modifications occurred as early as those in biochemical parameters of inflammation such as serum fibrinogen and ceruloplasmin, and preceded the appearance of any clinical symptom of the disease. Inverse correlations were found between plasma zinc levels and these two biochemical indices. Other modifications in trace element levels were observed two weeks after disease induction, the most important being a considerable increase in liver copper levels. Although food intake of affected animals decreased with the progression of the disease, there was no evidence of depletion in zinc and copper levels over the study period. A redistribution of body zinc between different biological compartments (mainly plasma and liver) occurred simultaneously with an accumulation of copper in several organs. The decreasing selenium status of animals was not clearly related to the inflammatory process.     

维生素E和锌对肝硬化大鼠脂质过氧化和糖代谢的影响

采用半乳糖胺(Galn)诱导大鼠肝硬化.其血清和肝中脂质过氧化物升高,谷胱甘肽氧化还原系统失衡;谷草转氨酶,羟脯氨酸水平升高;肝铜蓄积,锌,铁丢失;血清锌,铜,铁含量升高,血糖,胰岛素,胰高血糖素代谢异常,补充维生素E可使谷草转氨酶,羟脯氨酸,脂质过氧化水平降低,肝脏和血中谷胱甘肽和微量元素维持平衡,并能纠正血糖,胰岛素,胰高血糖素代谢紊乱,减轻肝损伤及纤维增生。肝硬化大鼠锌代谢异常,大剂量或长时间不适当补锌同样可以造成肝锌,铜,铁比例失调,对保护肝脏免受Galn毒性及纠正血糖,胰岛素,胰高血糖素代谢紊乱产生不利影响。     

A new diagnostic method for chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma based on serum metallothionein, copper, and zinc levels

Serum metal levels and their ratios are frequently reported to be good signals for diagnosing various diseases. These parameters are not always specific to the disease, however, it is necessary to use other serum parameters for an exact diagnosis. We examined whether the monitoring of these serum parameters such as metallothionein, copper, and zinc levels are useful in diagnosing hepatic disorders. Metallothionein levels of patients with liver cirrhosis and hepatocellular carcinoma were found to be significantly lower than those of patients with chronic hepatitis and those of controls. In contrast, copper levels of the patients with liver cirrhosis and hepatocellular carcinoma were significantly higher than those with chronic hepatitis and controls. Zinc levels of the patients with chronic hepatitis and hepatocellular carcinoma were lower than those of controls. Using these three parameters, we are introducing a new parameter, (Cu/Zn)/MT, by which we can discriminate between patients in the [control+miscellaneous diseases+chronic hepatitis] group and those in the [liver cirrhosis+hepatocellular carcinomal group. The new parameter does not, however, allow us to clearly distinguish between the liver cirrhosis and hepatocellular carcinoma groups. Multivariate discriminant analysis was found to be very useful, with combinations of two discriminant functions having been designed to discriminate both between chronic hepatitis and liver cirrhosis and between liver cirrhosis and hepatocellular carcinoma. This method recognizes the differences between hepatic disorder, including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma groups. On the basis of these results, we propose here that the diagnosis of hepatic disorders should be made based on a combination of three serum levels such as those of metallothionein, copper, and zinc.     

寻常性银屑病患者血清高迁移率蛋白和血管内皮细胞钙黏蛋白的含量变化

目的 探讨血清高迁移率蛋白1( HMGB1)和血管内皮细胞钙黏蛋白(VE-cadherin)含量变化在寻常性银屑病发病中的作用及其临床意义.方法 应用双抗体夹心酶联免疫吸附法( ELISA)检测78例寻常性银屑病患者外周血清中HMGB1和VE-cadherin的水平;并动态观察治疗前后HMGB1和VE-cadherin的变化及其与寻常性银屑病患者病情活动指标之间的关系.结果 寻常性银屑病患者血清中HMGB1和VE-cadherin的水平[分别为(2.58 ±0.19)、(4.22±0.81) mg/L]较正常对照组[分别为(0.86±0.12)、(2 87±0.64)mg/L]明显增高(t=10.53,10.16,P＜0.01).进行期与静止期的寻常性银屑病患者血清中HMGB1和VE-cadherin水平[进行期患者分别为(3.58±0.17)、(5.62±0.85) mg/L,静止期分别为(2.07±0.12)、(3.45±0.82) mg/L]差异有统计学意义(t=12.47,13.11,P＜0.01).治疗中、治疗后血清HMGB1、VE-cadherin水平及银屑病面积与严重性指数(PASI)评分与治疗前相比明显下降[治疗前分别为(2.58±0.19)、(4.22±0.81) mg/L,(12.0±4.2)分;治疗中分别为(1.98±0.17)、(3.45±0.79) mg/L,(8.38±3.49)分;治疗后分别为(0.92±0.19)、(2.94±0.65) mg/L,(2.67±0.87)分],差异有统计学意义(治疗中与治疗前相比,t=7.48、7.92、6.67,P＜0.05;治疗后与治疗前相比,t=12.72,11.48,13.13,P＜0.01).治疗后与对照组相比,HMGB1和VE-cadherin的水平差异无统计学意义.寻常性银屑病患者治疗前后血清HMGB1和VE-cadherin水平及银屑病PASI评分呈明显正相关(r=0.58,0.64,P＜0.01);同时VE-cadherin水平与银屑病PASI评分呈明显正相关(r=0.53,P＜0.01).结论 HMGB1和VE-cadherin在寻常性银屑病的发病中可能起重要作用,血清HMGB1和VE-cadherin的检测可作为反映寻常性银屑病病情活动的指标之一.     

近视儿童与青少年血清锌、铜、硒浓度的变化研究

目的:探讨近视儿童和青少年血清锌、铜、硒浓度和铜/锌比的变化及临床意义。方法:选取2018年1月至2020年12月间,内蒙古医科大学附属医院眼科门诊诊治的166例近视儿童与青少年［近视组,其中110例男孩,56例女孩,年龄（13.78±4.21）岁］以及150例同期视力正常的儿童与青少年［对照组,其中87例男孩,63例...     

Biliary excretion of copper and zinc in the rat as influenced by diethylmaleate, selenite and diethyldithiocarbamate

An i.p. diethylmaleate injection (dose: 3.9 mmoles/kg) decreased the endogenous Cu and Zn excretion in rat bile to 20 and 50 per cent, respectively. A corresponding decrease of the glutathione levels in liver and bile suggests that copper, at least partly, is excreted in the bile as a glutathione complex or by other glutathione-dependent mechanisms. Similar mechanisms may also be active for Zn. Selenite (5 μmoles/kg) or diethyldithiocarbamate (67 μmoles/kg) treatment also decreased the copper excretion in bile, but the glutathione levels were not significantly affected. These agents may act either by forming metal complexes which are not excreted, or by blocking the transfer of the metal from proteins to glutathione in liver cells. Selenite and diethyldithiocarbamate did not significantly influence the excretion of zinc in the bile. Orally given selenite (5.7 or 57 μmoles/100 g food) increased the kidney levels of copper and zinc and decreased liver levels of zinc, whereas diethyldithiocarbamate (6.7 mmoles/100 g food) caused increased liver content of copper and decreased kidney levels of copper and zinc. The differences observed between copper and zinc distribution when using selenite or diethyldithiocarbamate treatment may be due to different organ retention of the metal complexes formed.