输注去白细胞血小板防止发热反应的细胞因子分析

<正>血小板输注为当前恶性肿瘤化疗和造血干细胞移植的患者最主要的支持治疗,去除白细胞单采血小板的推广应用,给需要多次输注血小板者,减少由制备与贮存过程中白细胞释放的细胞因子而引起非溶血性发热输血反应(febrile non-hemolytic transfusionreactions,FNHTR)〔1-2〕。本文对输注去白细胞单采血小板后恶性肿瘤化疗和造血干细胞移植患者细胞因子(IL-6、IL-8、TNF-β、     

冠心病患者血浆ET、TNF-α、IL-6及血小板参数的研究

目的 探讨内皮素(ET)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、血小板参数在冠心病发病中的临床意义,以期提高诊治及预后.方法 选择2007年7月-2008年2月就诊于山西省人民医院并确诊的66例冠心痛住院患者,并检测血浆ET含量,血清TNF-α、IL-6及血小板参数值.结果 冠心病组ET、TNF-α、IL-6、平均血小板体积(MPV)及血小板分布宽度(PDW)值显著高于32例同期正常对照组,差异具有统计学意义(P<0.01),血小板计数(PLT)低于正常对照组.结论 ET、TNF-α、IL-6、血小板参数对冠心痛的诊断治疗具有重要指导意义.     

rhTNFR:Fc对类风湿关节炎患者血清中致炎及抗炎性细胞因子的影响

目的探讨重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白[rhTNFR：Fc，益赛普（etanercept）]治疗类风湿关节炎（RA）的免疫学机制，了解rhTNFR：Fc对RA患者血清中致炎及抗炎性细胞因子的作用，比较rhTNFR：Fc和甲氨蝶呤（MTX）对这些细胞因子作用的不同，为rhTNFR：Fc治疗RA提供进一步的实验依据。方法采用酶联免疫吸附试验（ELISA）检测RA患者rhTNFR：Fc治疗前后血清中白细胞介素（IL）-1、IL-6、IL-1、肿瘤坏死因子（TNF）-α和干扰素ONF）-γ度。观察rhTNFR：Fc对细胞因子的影响。结果①RA患者血清中的炎性细胞因子TNF-α、INF-γ、IL-1β、IL-6的水平均高于健康对照组，其中IL-6、INF-γ、TNF-α的水平与健康对照组相比差异有统计学意义（P〈0．01），抗炎性细胞因子IL-10的水平低于健康对照组，但两者相比差异无统计学意义（P〉0．05）。②rhTNFR：Fc治疗RA后血清中Th1型细胞因子TNF-α、INF-γ、IL-1β、IL-6水平均明显降低（P〈0．01），而Th2型细胞因子IL-10的水平明显提高（P〈0．01）。③RA患者血清中TNF-α水平与肿胀关节数及肿胀关节指数呈正相关（P〈0．05）。④对临床指标的分析表明，rhTNFR：Fc治疗RA疗效确切。结论rhTNFR：Fc可以降低RA患者血清中TNF-α、INF-γ、IL-1β、IL-6的水平，同时升高IL-10水平；在抑制了Th1型细胞因子的同时．增强了Th2型细胞因子的效应．从而改善RA的病情。     

流行性腮腺炎合并急性胰腺炎患者血清细胞因子水平的变化

目的：观察流行性腮腺炎合并急性胰腺炎患者的血清细胞因子水平变化。方法应用悬液芯片法检测流行性腮腺炎合并急性胰腺炎及单纯流行性腮腺炎患者的血清IL-2、IL-4、IL-6、IL-8、IL-10、干扰素γ（ INF-γ）、肿瘤坏死因子-α（ TNF-α）水平;血细胞分析仪检测白细胞数,速率法检测血尿淀粉酶及血脂肪酶。收集流行性腮腺炎合并胰腺炎患者临床资料,发病24 h内行APACHEⅡ评分,48 h内行Ranson评分。结果 APACHEⅡ及Ranson评分结果显示急性胰腺炎患者均为轻症。流行性腮腺炎合并急性胰腺炎患者血清IL-6、IL-8、TNF-α、血尿淀粉酶及血脂肪酶高于单纯流行性腮腺炎患者（P均＜0．05）。结论血清高水平的IL-6、IL-8、TNF-α可能参与了流行性腮腺炎感染后急性胰腺炎的形成。     

细胞因子与鼻息肉发病的关系

目的探讨鼻息肉发病与机体内炎症细胞因子含量表达的相关性。方法应用酶联免疫吸附实验双抗体夹心法检测鼻息肉患者血浆中转化生长因子(TGF)-β、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β及IL-6的表达,分析鼻息肉发病与炎症细胞因子含量的关系。结果鼻息肉患者血浆中TGF-β、TNF-α、IL-1β及IL-6的表达水平明显升高,与鼻中隔偏曲及健康对照组相比差异有统计学意义(P<0.01)。结论鼻息肉患者血浆中炎症细胞因子表达水平与疾病的发生发展密切相关。     

细胞因子与冠状动脉狭窄程度的相关性研究

目的 探讨细胞因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和γ干扰素(IFN-γ)在冠状动脉病变中的作用。方法 将93例冠心病患者根据冠状动脉狭窄程度分成三组：轻度组，34例；中度组，33例；重度组，26例。采用酶联免疫吸附法检测外周血各炎症细胞因子水平，并与30例年龄和性别相当的健康体检者进行比较。结果冠心病组外周血IL-1β、IL-6、TNF-α和IFN-γ水平均显著高于对照组各项指标，而且增高程度与冠状动脉狭窄程度一致，血管重度狭窄组细胞因子增高尤为明显。结论 研究显示：细胞因子IL-1β、IL-6、TNF-α和IFN-γ与冠状动脉狭窄呈显著正相关，提示细胞因子可能损伤血管内皮细胞，使血管内皮功能失调，导致冠状动状血管狭窄。同时也为判断冠状动脉狭窄病变程度指标而提供了一条线索。     

慢性肾小球肾炎与细胞因子含量表达的相关性

目的探讨慢性肾小球肾炎发病与机体内细胞因子含量表达的相关性。方法应用酶联免疫吸附实验双抗体夹心法检测慢性肾小球肾炎患者血浆中转化生长因子(TGF)-β、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β及IL-6的含量表达。并进行肾脏B超检查,详细记录肾脏形态学改变。结果 TGF-β、TNF-α、IL-1β、IL-6含量表达水平明显升高,与肾功能损害程度呈正相关。结论慢性肾小球肾炎患者血浆中炎症细胞因子含量表达水平与疾病的发生发展密切相关。临床上通过阻断炎症细胞因子的生物学效应可改善或减轻慢性肾小球肾炎患者肾功能损害。     

60 Coγ线辐照全血对T淋巴细胞分泌细胞因子的影响

目的：探讨^60Coγ射线辐照全血对T淋巴细胞分泌细胞因子白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）及肿瘤坏死因子（TNF）的影响。方法：分别用5种剂量的^60Coγ射线对新鲜全血进行辐照处理，然后用免疫标记法区分T淋巴细胞CD3^＋、CD4^＋和CD8^＋ 3个亚群，用流式细胞术检测辐照前后3种表型T细胞内分泌IL-2、IFN-γ及TNF的淋巴细胞数量的百分率的变化。结果：综合5个剂量组辐照新鲜全血后3种表型的T细胞分泌表达的IL-2、INF-γ、TNF均有明显降低（P〈0．01）；经15Gy辐照后，3种表型的T细胞分泌表达的上述细胞因子的百分率降低均不明显（P〉0．05）；但从20～35Gy中，除了20Gy CD3^＋、CD4^＋及25GyCD3^＋T细胞内分泌表达的TNF降低不明显（P〉0．05）外，其余剂量辐照后对3种表型的T细胞内分泌细胞因子均有所降低（P〈0．05或0．01）。结论：经^60Coγ线辐照新鲜全血能抑制T细胞内分泌表达细胞因子，且抑制效应与辐照剂量相关，辐照剂量越大，抑制效应越明显。     

超声弹性成像技术联合炎症因子在非特异性慢性腰痛诊断中的应用

目的 探讨超声弹性成像技术联合炎症因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α、干扰素(INF)-γ在非特异性慢性腰痛中的诊断价值。方法 纳入118例非特异性慢性腰痛患者为病例组，纳入112例正常健康志愿者为对照组。对非特异性慢性腰痛患者进行超声弹性成像检查获取杨氏模量值，同时进行疼痛视觉模拟评分(VAS)、日本骨科协会(JOA)评分评估，采用流式荧光发光法检测血清IL-1β、IL-6、TNF-α、INF-γ水平；采用Pearson相关性分析检验非特异性慢性腰痛患者两侧杨氏模量值、炎症因子与VAS、JOA评分的相关性；采用受试者工作特征(ROC)曲线分析两侧杨氏模量值、炎症因子水平对非特异性慢性腰痛的诊断价值。结果 病例组左右两侧杨氏模量值、IL-1β、IL-6、TNF-α、INF-γ水平高于对照组，差异有统计学意义(P<0.05)。非特异性慢性腰痛患者两侧杨氏模量值、IL-1β、IL-6、TNF-α、INF-γ与VAS呈正相关，与JOA评分呈负相关(P<0.05)。左侧杨氏模量值、右侧杨氏模量值、IL-1β、IL-6、TNF-α、INF-γ水平单独及...     

原发性高血压患者血压昼夜节律与细胞因子的相关性研究

目的观察昼夜血压呈杓型、非杓型分布的高血压病患者血浆中细胞因子的变化,探讨细胞因子与血压昼夜节律的相关性。方法经动态血压监测将84例高血压病患者分为杓型组35例,非杓型组49例,采用双抗体夹心ELISA法分别测定血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ),可溶性白细胞介素-2受体(sIL-2R)水平。结果非杓型组与杓型组比较,IL-6、TNF-α、sIL-2R明显升高(P<0.001;P<0.001;P<0.001),IFN-γ明显降低(P<0.01);夜间血压下降与IL-6、TNF-α、sIL-2R负相关[收缩压(SBP)与IL-6:r=-0.347,P<0.01;SBP与TNF-α:r=-0.427,P<0.005;SBP与sIL-2r:r=-0.309,P<0.01;舒张压(DBP)与IL-6:r=-0.292,P<0.05;DBP与TNF-α:r=-0.462,P<0.005;DBP与sIL-2R:r=-0.278,P<0.05];夜间血压下降与IFN-γ正相关(SBP与IFN-γ:r=0.263,P<0.05;DBP与IFN-γ:r=0.241,P<0.05)。多元线性回归分析,IL-6、TNF-α、sIL-2R与夜间血压下降直线相关(SBP:F=6.742,P<0.005;DBP:F=6.138,P<0.005)。结论血压昼夜节律异常的高血压病患者存在细胞因子水平升高,细胞因子可能参与血压昼夜节律的调节。     

急性冠状动脉综合征患者外周血细胞因子的检测及临床意义

目的　通过检测急性冠状动脉综合征 (ACS)患者血清中细胞因子白介素 - 1β(IL- 1β)、白介素 - 2受体 (IL-2 R)、白介素 - 6 (IL- 6 )、肿瘤坏死因子 - α(TNF- α)、干扰素 - γ(IFN- γ)的变化 ,旨在探讨 ACS的发病机制。方法　选取ACS患者 80例 ,其中急性心肌梗死 (AMI)患者 4 0例 ,不稳定型心绞痛 (U AP)患者 4 0例 ,ACS患者经治疗 4个月后进行随访 ,同时选取正常对照 4 0例。采用酶联免疫法 (EL ISA)测定上述病例和对照血清中的 IL - 1β、IL - 2 R、IL - 6、TNF-α和 IFN-γ水平 ,并进行统计学分析。结果　 1.除 IFN-γ外 ,IL - 1β、IL - 2 R、IL - 6、TNF-α均为 AMI组、U AP组显著高于对照组 ,并在随访时明显降低。 2 .采用 Wagner37项 / 2 9分 QRS积分系统对 4 0例 AMI患者进行梗死面积的估计 ,结果发现 QRS积分≤ 6分组和 >6分组之间细胞因子水平无明显差异。结论　外周血中 IL- 1β、IL-2 R、IL- 6、TNF- α水平可能作为诊断和预测 ACS发生的敏感指标 ,并可以反应病情的稳定情况。血清中炎性指标的升高主要不是心肌坏死的作用 ,可能与多病灶的不稳定斑块的存在有关。     

Relative expression and correlation of tumor necrosis factor-α, interferon-γ, and interleukin-17 in the rheumatoid synovium

Although tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-17 (IL-17) play important roles in RA, their relative expression and possible correlation in synovial tissues are not well understood. In this study, mRNA expression levels of IFN-γ, IL-17, and TNF-α were investigated in individual patients with RA and the correlations between pairs of these three pro-inflammatory cytokines were analyzed. Synovial tissues were obtained during arthroplasties from 24 joints of 24 RA patients. After harvesting synovial tissues, total RNA was isolated then quantitative real-time polymerase chain reaction (qRT-PCR) for IFN-γ, IL-17, and TNF-α was performed. Correlation of expression levels between them was also analyzed. Expression levels of TNF-α, IFN-γ, and IL-17 in patients receiving TNF inhibitors (TNFi) and those treated with conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) alone were also compared between groups. Based on relative expression levels of the three pro-inflammatory cytokines, patients were classified into three major types; an IFN-γ plus TNF-α-dominant type, an IL-17-dominant type, and the other type. TNF-α expression levels were correlated with IFN-γ. In addition, there was a negative correlation between TNF-α and IL-17, and IFN-γ and IL-17. Median relative expression levels of TNF-α have no significant difference between the TNFi and the csDMARDs groups. In the rheumatoid synovial tissues, expression levels of TNF-α were modulated in parallel with IFN-γ, and TNF-α and IL-17, or IFN-γ and IL-17 did not co-express at high levels. This characteristic expression pattern of the three pro-inflammatory cytokines may be clinically useful information in the current cytokine-targeted treatment with biological DMARDs for RA.     

组蛋白去乙酰化酶1与乙型病毒性肝炎病情的关系

目的：观察不同类型乙型病毒性肝炎患者中炎性因子的表达及组蛋白去乙酰化酶1（HDAC1）的表达,探讨其在不同程度肝损伤患者中发生的意义。方法：收集临床乙型病毒性肝炎重型、重度、轻度患者及健康人外周血标本,分离血清及单个核细胞（PBMC）,用ELISA方法检测各组血清中IL-4、TNF-α、IFN-γ的表达及PBMC中HDAC1的表达,用RT-PCR方法检测PBMC中HDAC1 mRNA的表达。结果：IL-4、TNF-α、IFN-γ与HDAC1蛋白表达水平的相关性较高,重型组与重度组之间以及轻度组与正常人组之间的炎性因子表达及HDAC1表达差异无显著性意义（P〉0.05）,与轻度组和正常人组相比,重型组和重度组的IL-4、TNF-α、IFN-γ及HDAC1的蛋白表达以及HDAC1 mRNA的表达均升高。结论：IL-4、TNF-α、IFN-γ等相关炎症因子与乙型病毒性肝炎的病情程度密切相关,HDAC1在炎症因子的表达过程中可能发挥重要作用。     

慢性阻塞性肺疾病急性加重期合并肺结核患者外周血清中IL-6、TNF-α和IFN-γ水平变化及临床意义

目的 探究慢性阻塞性肺疾病急性加重期(AECOPD)合并肺结核患者外周血清中IL-6、肿瘤坏死因子α(TNF-α)和干扰素γ(IFN-γ)水平变化及临床意义.方法 将研究对象分为AECOPD合并肺结核组、AECOPD组、肺结核组和健康对照组,根据肺结核患者影像学检查结果将肺结核患者分为单侧病变组、双侧病变组、无空洞组和有空洞组,根据AECOPD合并肺结核患者治疗后的情况分为好转组和恶化组.采用ELISA法检测各组患者血清中的IL-6、TNF-α和IFN-γ水平.结果 AECOPD合并肺结核组血清中的IL-6和TNF-α水平显著高于AECOPD组和肺结核组(P＜0.05);单侧病变组患者血清中的IL-6、TNF-α和IFN-γ的水平显著低于双侧病变组(P＜0.05);无空洞组患者血清中的IL-6、TNF-α和IFN-γ的水平显著低于有空洞组(P＜0.05);病情好转组治疗后IL-6、TNF-α和IFN-γ水平显著低于治疗前(P＜0.05).结论 IL-6、TNF-α和IFN-γ在AECOPD合并肺结核的发病机制中起着一定的作用,其外周血清中水平的动态变化在一定程度上可以反映AECOPD合并肺结核病情的发展与转归.     

More Evidence of the Link of Interleukin-6 and Interleukin-10 with Critical COVID-19: A Report in Mexican Patients

Background: According to the World Health Organization, Mexico presents one of the highest mortality rates due to coronavirus disease 2019 (COVID-19). The "cytokine storm" phenomenon has been proposed as a pathological hallmark of severe COVID-19. Objective: To determine the association of serum cytokine levels with COVID-19 severity. Methods: We studied the cytokines IL-2, IL-4, IL-6, IL-10, TNF-α, and the IFN-γ serum levels through flow cytometry in 56 COVID-19 patients (24 critical and 32 non-critical) from Northwest Mexico. Results: We observed a significant increase in the IL-6 and the IL-10 levels in the sera of critical patients. These cytokines were also associated with mechanical ventilation necessity and death, IL-6 showing AUC values above 0.7 for both variables; and correlated with Na+, creatinine, and platelet levels. On the other hand, no association was found between IL-2, IL-4, TNF-α, and IFN-γ with tested variables. Conclusion: Our results corroborate previous observations regarding IL-6 and IL-10 involvement in the severity of COVID-19.     

姜黄素抑制超抗原TSST-1诱导脾细胞炎症因子的作用

目的 观察姜黄素对毒性休克综合征毒素-1(TSST-1)诱导的小鼠脾细胞炎症因子的影响,为进一步探讨姜黄素对炎症性休克的作用提供依据。方法 采用乳酸脱氢酶(LDH)释放法检测不同剂量的TSST-1和姜黄素的细胞毒作用,ELISA和流式细胞术检测相关炎症因子。结果 所选剂量TSST-1和姜黄素均未发现有明显细胞毒作用。TSST-1诱导脾细胞产生了大量的IFN-γ和IL-2,产生了少量的TNF-α,未能诱导出IL-1β、IL-6和IL-12。TSST-1诱导的Th1细胞因子产生不完全依赖于IL-12。姜黄素对IFN-γ和TNF-α的抑制作用与剂量有关,在15 μmol/L抑制效果最显著(P<0.05),但对IL-2无显著抑制作用(P>0.05)。结论 姜黄素能显著降低TSST-1诱导小鼠脾细胞产生的IFN-γ和TNF-α,但对T细胞增殖无明显抑制作用。     

麝香保心丸对心力衰竭大鼠Th1和Th2型细胞因子水平的影响

目的 探讨麝香保心丸对心肌梗死后心力衰竭大鼠血清Th1型细胞因子、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)和Th2型细胞因子白介素-4(IL-4)、白介索-10(IL-10)水平的影响.方法 结扎大鼠左冠状动脉前降支制作心肌梗死后心力衰竭模型,54只Wistar大鼠随机分为正常对照组、假手术组、心力衰竭模型对照组(模型组)、麝香保心丸小剂量组(小剂量组)、麝香保心丸大剂量组(大剂量组)和阳性药物对照组(阳性对照组),采用灌胃法给药.超声心动图检测用药前后大鼠心功能,放射免疫法测定血清TNF-α、IL-10水平,酶联免疫吸附法测定血清IFN-γ、IL-4水平.结果 用药前,假手术组与正常对照组左心室射血分数(LVEF)比较,差异无统计学意义;与正常对照组和假手术组比较,模型组、小剂量组大剂量组和阳性对照组大鼠LVEF降低(均P＜0.01);用药后,与假手术组比较,模型组的TNF-α和IFN-γ水平升高(P＜0.01);IL-4和IL-10水平降低(P＜0.01);与模型组比较,大剂量组的LVEF改善(P＜0.05),TNF-α和IFN-γ水平降低(P＜0.05,P＜0.01),IL-4和IL-10水平升高(P＜0.05),小剂量组的IL-10水平升高(P＜0.05),IFN-γ水平下降(P＜0.01);阳性对照组IL-10水平升高(P＜0.05),TNF-α和IFN-γ水平下降(P＜0.05,P＜0.01);小剂量组和阳性对照组的IL-4水平有升高趋势,LVEF有下降趋势,但差异均无统计学意义.结论 麝香保心丸治疗改善了心肌梗死后心力衰竭大鼠的左心功能,大鼠血清IL-4、IL-10水平升高,TNF-α、IFN-γ降低.

Abstract：

Objective To investigate the effects of Shexiangbaoxin pills (SXBXP) on the serum levels of T helper type 1 (Th1) cytokines including tumor necrosis factor-a (TNF-α),interferon-γ(IFN-γ) and T helper type 2 (Th2) cytokines incloding interleukin-4 (IL-4),interleukin-10 (IL-10)in rats with chronic heart failure (CHF).Methods The CHF models were established by left anterior descending coronary artery ligation.Fifty-four Wistar rats were randomly divided into six groups:control group,sham operation group,CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group.Heart function was examined by echocardiography before and after therapy,the TNF-α and IL-10 levels were measured by radioimmunoassay,IFN-γ and IL-4 levels were measured by enzyme linked immunosorbent assay (ELISA).Results (1) Before therapy,there was no significant difference between control group and sham operation group in ejection fraction of left ventricle (LVEF).Compared with control group and sham operation group,the levels of LVEF were significantly decreased in CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group (all P＜0.01).(2) After therapy,compared with sham operation group,the serum levels of TNF-α and IFN-γ were markedly increased,and IL-4 and IL-10 were significantly decreased in CHF model group (all P＜0.01 ).Compared with CHF model group,LVEF was significantly improved,serum levels of TNF-α and IFN-γ were markedly decreased,IL-4 and IL-10 were markedly increased in large dose SXBXP group (all P＜0.05).In small dose SXBXP group,serum levels of IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In positive medicine group,serum levels of TNF-α and IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In small dose SXBXP group and positive medicine group,serum levels of IL-4 increased and LVEF decreased,even though there was no significant difference compared with CHF model group.Conclusions The SXBXP therapy of rats with CHF induced by myocardial infarction improves the heart function,decreases the serum levels of TNF-α and IFN-γ,and increases serum levels of IL-4 and IL-10.     

肺结核患者外周血sIL-2R、TNF-α、IFN-γ、IL-6的检测及意义

目的 探讨肺结核患者的血清可溶性白介素-2受体（sIL-2R）、γ干扰素（IFN-γ）、肿瘤坏死因子α（TNF-α）、白介素-6（IL-6）的变化及意义。 方法 采用双抗体夹心ABC-ELISA法测定214例初治肺结核的血清中sIL-2R、TNF-α、IL-6、IFN-γ的水平。 结果 肺结核病人血清sIL-2R的检测结果各组平均值均高于正常,随着病灶范围增加sIL-2R的水平也增加;3～4肺野组与5～6肺野组sIL-2R水平明显高于1～2肺野组(P<0.01);痰菌阳性组sIL-2R水平明显高于阴性组（P<0.01）。肺结核病人血清TNF-α的检测结果显示1～2肺野组与3～4肺野组平均值均高于正常,随着病灶范围增加TNF-α的水平减少,5～6肺野组在正常范围,1～2肺野组TNF-α的水平明显高于5～6肺野组(P<0.01);无空洞组TNF-α平均值高于有空洞组(P<0.05)。肺结核病人的血清INF-γ的检测结果显示各组平均值均高于正常,其中5～6个肺野组比3～4肺野组高(P<0.05);痰菌阳性组比阴性组IFN-γ水平高(P<0.05)。 结论 不同肺结核患者外周血中sIL-2R、TNF-α、IFN-γ、IL-6水平存在着差异,这些细胞因子可能在结核病的发病中起着一定的作用。     

IL-10, TNF-α and IFN-γ levels in serum and stomach mucosa of Helicobacter pylori-infected patients

H. pylori is a human pathogen that colonizes the epithelium of the stomach. The host immune response may influence the disease process, where cytokines play important roles in the development of disease. In this study, the concentrations of selected cytokines in the gastric antrum and stomach body mucosa and also in the serum were evaluated. Eighty patients according to their rapid urease test were divided into two groups: H. pylori positive (n=39) and H. pylori-negative (n=41). The concentrations of cytokines in biopsies and serum were determined by ELISA method. The mean TNF-α and IFN-γ levels in the infected group were significantly higher than that of uninfected patients. In contrast, IL-10 level in most patients was undetectable. The mean antral of stomach TNF-α and IFN-γ levels were significantly higher than that of the stomach body. IFN-γ serum level showed positive correlation with antrum and stomach body levels, whereas no correlation was found in TNF-α in different samples. Higher levels of TNF-α and IFN-γ in antral indicate that the colonization of bacteria in the antrum may be higher than stomach body (culture results from two sites support this statement). Increased serum level of IFN-γ indicates the activation of circulating-T cells against infection. Induced H. pylori-related TNF-α is concentrated is gastric mucosa and this pathogen does not cause any significant change in the serum level of this cytokine. Therefore H. pylori by inducing certain inflammatory cytokines but not IL-10 may contribute the process of disease development.     

IL-6 and IL-10 Closely Correlate with Bacterial Bloodstream Infection

Background: Given the high mortality of bacterial bloodstream infections (BSI), blood culture results do not meet clinical needs timely due to being time-consuming and having low positive rate. Whether we can identify the severity and type of bacterial infections by cytokines is a controversial issue. Objective: To investigate the dynamic change of cytokines in BSI. Methods: 55 patients with Gram-positive (GP) BSI, 64 patients with Gram-negative (GN) BSI and 52 healthy controls were enrolled. We quantitatively detected the cytokines interleukin (IL)-2, IL-4, IL-6, IL-10, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) by flow cytometry in the sera. The levels of procalcitonin, C-reactive protein, leukocytes and neutrophils were also detected simultaneously. Results: There were significantly up-regulated IL-6 and IL-10 expression in BSI patients, particularly in the GN-BSI, for instance Escherichia coli and Klebsiella pneumoniae infections; following the treatment, IL-6 and IL-10 decreased by 10-23 and 4-27 times, respectively. Additionally, IL-2, TNF-α and IFN-γ expression increased slightly in BSI patients and IFN-γ expression declined as GN-BSI progressed. Conclusion: IL-6 and IL-10 are closely associated with the severity and treatment efficacy of BSI, and can help to distinguish between GP-BSI and GN-BSI at an early stage.