碱性成纤维细胞生长因子与小儿烧伤创面的愈合

背景：皮肤创面愈合是一个复杂的病理过程,对于创伤和创伤后感染等引起皮肤难愈合的研究一直是临床创面修复的难题,对于碱性成纤维细胞生长因子促进皮肤创面愈合的基础研究相对较多,临床应用研究较少。 目的：对碱性成纤维细胞生长因子促进皮肤创面愈合研究的文献资料趋势进行多层次分析,探讨在小儿烧伤创面愈合中的应用疗效。 方法：以电子检索方式对CNKI数据库学术期刊2002-01/2011-12收录有关碱性成纤维细胞生长因子促进皮肤创面愈合研究的文献进行分析,采用检索词为“碱性成纤维细胞生长因子;创面愈合”,运用数据库的分析功能和Excel软件图表的功能分析数据特征。 结果与结论：CNKI数据库学术期刊2002/2011收录碱性成纤维细胞生长因子促进皮肤创面愈合研究的文献共228篇,文献数量处于平稳发展趋势。《中国组织工程研究与临床康复》杂志收录的文献数量最多为26篇。解放军第304医院产出的文献最多。碱性成纤维细胞生长因子促进皮肤创面愈合研究文献的基金资助项目有16项,基金资助项目的文献共76篇,以国家重点基础研究发展计划(973)项目和国家自然科学基金资助项目的文献最多。碱性成纤维细胞生长因子在小儿烧伤创面愈合中应用的文献虽然较少,但实验结果均显示治疗效果较好,有促进小儿Ⅱ度烧伤创面愈合的作用,而且无不良反应出现。     

脐带间充质干细胞促血管新生在治疗下肢缺血中的研究与应用

背景：干细胞在一定诱导条件下可分化成为血管性内皮细胞,促进缺血下肢血管新生和有效循环的建立,改善糖尿病远端缺血肢体供血。目的：概述脐带间充质干细胞生物学特性、促血管新生的机制,探讨脐带间充质干细胞修复神经病变及慢性创面等基础研究和临床研究现状。方法：检索2000至2015 年PubMed 数据库、维普中文科技数据库及万方数据相关文献。英文检索词为“stem cells transplantation,umbilical Cord Mesenchymal Stem Cell,diabetic angiopathies”;中文检索词为“干细胞移植,脐带间充质干细胞,糖尿病血管病变”。结果与结论：与目前外周血干细胞及骨髓间充质干细胞移植相比,脐带间充质干细胞来源更广泛,采集方便,扩增能力强,无免疫原性,不存在伦理学争议,成为促血管新生和基因治疗缺血性疾病的理想靶细胞和种子细胞。脐带间充质干细胞能够分化为血管内皮细胞和成纤维细胞参与创面愈合,也可促进神经营养因子表达,促进缺血组织神经再生,并通过旁分泌与自分泌细胞因子、抗炎及免疫调节等参与受损组织的组织修复,加快溃疡面愈合,对于改善糖尿病下肢缺血、修复糖尿病周围神经病变和促进慢性溃疡创面愈合等治疗中具有广阔的应用前景。与单纯的干细胞移植比较,脐带间充质干细胞联合细胞因子基因治疗可进一步提高干细胞存活率及促进血管新生。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

干细胞分泌因子促进难愈性创面愈合的机制及应用前景

背景：干细胞能够分泌多种伤口愈合相关因子。 目的：总结并分析干细胞分泌的创面愈合相关因子治疗难愈性创面的可行性,及其可能的调控及修复机制。 方法：应用计算机检索Google、Pubmed及万方数据库相关文献,英文检索词为“chronic wound,refractory wound,cutaneous,wound healing,wound repair,diabetic wound,stem cell-derived conditioned medium,cellular cytokine solution”。中文检索词为“创面修复,创伤修复”。选择论点论据可靠且分析全面的与干细胞分泌的促进伤口愈合因子密切相关的文章,排除内容重复文献,最终纳入34篇文献进行总结综述。 结果与结论：多种干细胞培养上清液中富含创面愈合相关因子,可作为细胞因子制剂治疗难愈性创面。然而由于干细胞来源不同,体外培养条件不同,其因子分泌状况也有所差异,因此应用何种来源的干细胞分泌因子,如何在体外最大限度地刺激干细胞产生创面愈合相关因子以及其未来在临床应用中的使用时机、频次和剂量仍有待进一步探索。     

创面愈合与瘦素促进的跨膜信号途径

背景：瘦素与其受体结合时,能以内分泌和旁分泌等方式促进新血管生成,调节炎症及免疫功能。 目的：探讨瘦素在创面愈合中的作用及跨膜信号传导机制。 方法：采用计算机检索CNKI数据库和PubMed数据库,以“瘦素,创面愈合,跨膜信号传导”和“leptin,wound healing,transmembrane singal”为关键词,语言设定为中文或英文。从瘦素在创面愈合的作用及跨膜信号传导2个方面进行总结。 结果与讨论：共检索到78篇文章,按纳入和排除标准对文献进行筛选,共纳入22篇文章。结果表明瘦素能够促进表皮角质形成细胞增殖、迁移,其跨膜信号传导涉及到JAK2,STST3,ERK1等多个信号分子。瘦素-受体信号的激活则改善创面愈合,其与瘦素促进表皮角质形成细胞增殖和再上皮化有关。     

生肌玉红胶原海绵修复创面损伤

背景：生肌玉红胶原海绵能显著促进创面愈合。 目的：观察生肌玉红胶原海绵对兔皮肤创面愈合的影响。 方法：在新西兰白兔背部制造3处全层皮肤缺损创面,分别以生肌玉红胶原海绵、贝复剂(重组碱性成纤维细胞生长因子外用溶液)、生理盐水修复。观察各组创面愈合时间、创面愈合率,检测创面修复组织内成纤维细胞数、血红素氧化酶1、转化生长因子β1 mRNA与蛋白及增殖细胞核抗原蛋白表达。 结果与结论：与贝复剂、生理盐水比较,生肌玉红胶原海绵可显著促进成纤维细胞增殖(P < 0.05),增加创面血红素氧化酶1、转化生子因子β1及增殖细胞核抗原的表达(P < 0.05),提高创面愈合率(P < 0.05),缩短创面愈合时间(P < 0.05)。证实生肌玉红胶原海绵能够促进创面修复,其作用机制可能是通过提高创面血红素氧化酶1、转化生长因子β1表达促进细胞增殖,增加成纤维细胞等创面修复细胞。     

结缔组织生长因子与关节软骨的修复

背景：结缔组织生长因子有刺激间充质细胞向软骨细胞分化的潜能,能促进软骨细胞的增殖和分化,可促进关节软骨细胞Ⅱ型胶原及蛋白多糖的表达,与其他生长因子一起在关节软骨修复的过程中发挥着重要作用。目的：重点就结缔组织生长因子的结构,在关节软骨修复中的功能,与其他物质的相互作用方面作一综述。方法：以“connective tissue growth factor,connective tissue growth factor and articular cartilage,articular cartilage damage,articular cartilage repairment”为英文检索词,以“关节软骨损伤”为中文检索词,检索PubMed数据库、中国知网-cnki数据库1980年1月至2014年7月有关关节软骨损伤修复的文献,排除与软骨损伤的修复重建相关性不强、以及内容重复、陈旧的文献。共保留32篇文献进行综述。结果与结论：结缔组织生长因子有刺激间充质细胞向软骨细胞分化的潜能,能促进软骨细胞的增殖、分化和成熟,可维持胞外基质合成以及平衡,可促进关节软骨细胞Ⅱ型胶原及蛋白多糖的表达,与其他生长因子一起在关节软骨修复的过程中发挥着重要作用。结缔组织生长因子是软骨细胞生长、增殖、分化的关键生长因子之一,贯穿软骨修复整个过程。研究发现,骨性关节炎患者的关节软骨细胞对成纤维细胞生长因子1和结缔组织生长因子表达呈现明显相关性增加。结缔组织生长因子通过对关节软骨组织的信号通路及与其他组织内的细胞因子彼此作用对关节软骨细胞及基质发挥作用。 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程全文链接：     

表皮干细胞在组织工程创面修复中的应用

背景：随着细胞生物学、分子生物学技术、组织工程学的快速发展,探寻组织工程化皮肤创面覆盖物的“种子细胞”的研究逐渐增多。 目的：总结表皮干细胞的生物学特性,探讨其在皮肤创面修复过程中的再生作用与临床应用价值。 方法：应用计算机检索CNKI和PubMed数据库中2002-07/2011-12关于表皮干细胞修复皮肤损伤研究的文章,以“表皮干细胞,创面修复,组织工程,皮肤”或“epidermal stem cells,tissue engineering skin,wound surface”为检索词进行检索。选择文章内容与表皮干细胞修复皮肤损伤研究进展有关的文章,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到129篇文献,根据纳入标准选择28篇文献进行综合分析。 结果与结论：表皮干细胞是表皮发生、分化和创面修复的基础,其正常增殖分化是维持皮肤正常组织结构和细胞内环境稳定的基本要求,也是皮肤组织工程理想的种子细胞。对烧伤、创伤等大面积皮肤缺损的治疗,对皮肤疾病的细胞疗法、基因治疗等方面都有很好的应用前景。表皮细胞的体外培养是复合人工皮肤组织工程学研究的先决条件。随着对表皮干细胞分离、纯化和培养技术的不断完善,可达到迅速构建表皮层的目的。但表皮干细胞的应用研究仍需要进一步的探索。     

自体富血小板血浆修复慢性难愈性创面的作用：回顾性研究和文献检索证据分析

文题释义： 慢性难愈性创面：通常把各种内外因作用下创面不能通过正常的创面愈合过程而达到愈合,呈现病理性炎症反应状态,从而导致创面经久难愈的称为慢性难愈性创面。 富血小板血浆：是通过离心自体血而提取出的含有高浓度血小板的血浆,除血小板外,其中含有多种高浓度的生长因子及纤维蛋白。 背景：自体富血小板血浆对难愈性创面有一定修复效果,可加快软组织愈合速度。而富血小板血浆凝胶可以防止血小板的流失,使血小板在局部长时间存活并分泌生长因子,更有利于创面愈合。 目的：观察自体富血小板血浆凝胶对慢性难愈性创面的修复作用,并分析其研究现状。 方法：①收集慢性难愈性创面病例22例,其中糖尿病性溃疡8例,创伤性溃疡3例,创伤后骨不连3例,电烧伤后骨外露2例,放射性溃疡6例,创面行清创手术后用富血小板血浆凝胶外用,定期换药并更换富血小板血浆凝胶,观察创面愈合情况;②由第一作者检索万方数据库近10年的有关自体富血小板血浆凝胶治疗慢性难愈性创面的相关文献,并分析研究现状。 结果与结论：①回顾性分析结果表明,富血小板血浆修复的22例慢性难愈性创面病例,创面完全愈合11例,创面缩小后经皮瓣转移后愈合5例,行游离植皮治疗获得愈合6例,患者均无疾病传播及免疫排斥反应,结果证实富血小板血浆凝胶对慢性难愈合伤口有明显的修复作用,安全有效;②文献检索结果显示,纳入文献32篇,其中2016年到2018年对自体富血小板血浆在慢性难愈性创面的研究发文量逐年升高,但现有证据尚缺乏说服力,缺乏大规模、多中心、前瞻性随机对照研究,如何规范富血小板血浆的制备及使用过程以保证疗效的稳定性还有待进行更多的探索。 ORCID: 0000-0002-2480-5159(李万同) 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

糖尿病皮肤慢性创面无细胞治疗的进展与问题北大核心

背景:糖尿病皮肤溃疡是糖尿病的难治并发症之一,发生后迁延不愈。以往研究发现,间充质干细胞具有组织修复和炎症免疫调节能力,并且相关研究表明间充质干细胞可能主要是通过旁分泌效应分泌各种细胞因子和外泌体等,发挥组织与创面修复作用。因而可使用生长因子、间充质干细胞条件培养基及外泌体等治疗糖尿病皮肤慢性创面,以直接或间接机制对伤口愈合产生积极影响。目的:总结生长因子、条件培养基和外泌体等促进糖尿病皮肤慢性创面修复的相关机制、应用效果,以及无细胞治疗在当前研究的局限性。方法:由第一作者检索PubMed及Web of Science数据库中收录的相关文献,检索词为“Diabetes,Diabetic foot,Diabetic foot ulcers,Chronic wound,Mesenchymal stem cells,Cell-free,Exosomes,Growth factors,Conditioned mediums,Wound healing,Tissue repair,Angiogenesis,Regeneration,Biomaterial”,文献检索时限为2005年1月至2022年2月,经过阅读并筛选整理,选择与综述内容相符的文献,最终确定整理出75篇文献进行综述。结果与结论:①间充质干细胞条件培养基、外泌体和生长因子可通过不同机制改善局部微环境,促进慢性创面的愈合;②通过条件培养基、外泌体和生长因子与新兴的组织工程生物学材料(如细胞支架、水凝胶等)相结合,可使其发挥更强的促进慢性创面愈合的作用;③基于间充质干细胞条件培养基、外泌体、生长因子的无细胞疗法是促进慢性创面修复的极有前景的治疗策略,要实现由临床前研究转化到临床研究,确保其用于患者治疗的安全性,还需要进行更多的研究,以查明相应的治疗作用机制。     

骨保护素和碱性成纤维细胞生长因子在牙周组织再生中的应用

背景：利用组织工程和基因治疗技术,联合应用骨保护素和碱性成纤维细胞生长因子可以促进牙周组织修复再生,是治疗重症牙周病的新方法。 目的：探讨联合应用骨保护素和碱性成纤维细胞生长因子以促进牙周组织修复再生的理论依据。 方法：应用计算机检索PubMed数据库(1996/2009)和中国知网数据库(1999/2009),分别以“osteoprotegerin,basic fibroblast growth factor,tissue engineering, gene therapy,periodontal regeneration”和“骨保护素、碱性成纤维细胞生长因子、组织工程、基因治疗、牙周组织修复再生”为检索词,通过阅读标题和摘要进行初筛,排除较陈旧和重复研究文献,保留符合纳入标准的文献29篇。 结果与结论：使牙槽骨、牙周膜和牙骨质获得再生,形成牙周新附着一直是口腔医学研究的热点。骨保护素是一种能阻止破骨细胞分化、促进骨形成的关键因子。碱性成纤维细胞生长因子在胚胎发育,血管生成,骨的形成和修复,促进细胞增生等有广泛的作用。2者都可促进牙周组织的修复和再生。组织工程技术和基因治疗技术的出现显著促进了牙周组织修复再生研究的发展,有选择地复合这2种生长因子促进牙周支持组织的再生和修复可成为治疗牙周病的一种新方法。     

Peripheral blood fibrocytes from burn patients: identification and quantification of fibrocytes in adherent cells cultured from peripheral blood mononuclear cells

Peripheral blood fibrocytes are a newly identified leukocyte subpopulation that displays fibroblast-like properties. These blood-borne cells can rapidly enter the site of injury at the same time as circulating inflammatory cells. We hypothesize that circulating fibrocytes represent an important source of fibroblasts for healing of extensive burn wounds where it may be difficult for fibroblasts to migrate from the edges of uninjured tissue. In this study we identified and quantified fibrocytes among the adherent cells cultured from human peripheral blood mononuclear cells (PBMC) obtained from 18 burn patients and 12 normal individuals, based on their ability to express type I collagen. Our results showed that adherent cells cultured from PBMC of burn patients differentiated to fibrocytes more efficiently than did those from normal individuals. The percentage of type I collagen-positive fibrocytes was significantly higher for patients than for controls (89.7 +/- 7.9% versus 69.9 +/- 14.7%, p < 0.001). This percentage was consistently higher for patients with a >/=30% total body surface area burn until 1 year, with the highest percentage appearing within 3 weeks of injury. A positive correlation was found between the levels of serum transforming growth factor-beta1 (TGF-beta1) and the percentage of fibrocytes developing in the cultures of PBMC derived from these patients. We also demonstrated that fibrocytes were derived from CD14(+) cells but not CD14(-) cells. Conditioned medium from CD14(-) cells was, however, required for fibrocyte differentiation, whereas direct contact between CD14(-) and CD14(+) cells was not necessary. Treatment of the cell cultures with TGF-beta1 enhanced the development of collagen-positive cells, whereas the inclusion of neutralizing anti-TGF-beta1 antibodies in the CD14(-) conditioned medium suppressed fibrocyte differentiation. These data suggest that the development of fibrocytes is up-regulated systemically in burn patients. Increased TGF-beta in serum stimulates the differentiation of the CD14(+) cell population in PBMC into collagen-producing cells that may be important in wound healing and scarring.     

细菌生物膜对慢性伤口的影响及防治策略

背景：了解细菌生物膜的形成及对伤口愈合的影响机制,防止细菌生物膜对伤口愈合的影响,对于提高慢性伤口的治愈率有重要作用。 目的：综述细菌生物膜对慢性伤口的影响机制,以及现有的治疗护理对策。 方法：应用计算机检索Springerlink及CNKI数据库2008至2013年发表的文章,检索关键词为“细菌生物膜,慢性伤口感染;bacterial biofilm,Chronic wound infection”。选取与伤口感染、细菌生物膜相关的治疗及护理方面相关的文章。 结果与结论：大量研究证实,致病菌或机会致病菌引起的慢性感染常与生物膜形成相关,细菌生物膜形成是导致慢性伤口难以愈合的重要因素之一。生物膜形成机制复杂。目前主要通过规范操作及环境管理、合理使用抗生素、及时清创来预防细菌生物膜的形成。针对细菌生物膜的治疗主要有超声治疗、负压治疗、噬菌体疗法、乳铁蛋白、硝酸镓、乙二胺四乙酸、木糖醇、蜂蜜、中药、高压氧治疗等。 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程全文链接：     

Differential expression of tissue inhibitors of metalloproteinases (TIMP-1, -2, -3, and -4) in normal and aberrant wound healing.

Wound healing is characterized by hemostasis, re-epithelialization, granulation tissue formation, and remodeling of the extracellular matrix. Matrix metalloproteinases and their specific inhibitors, TIMPs, contribute to these events. We investigated a total of 47 samples of normally healing wounds, chronic venous ulcers, ulcerative vasculitis, and suction blisters using immunohistochemistry and in situ hybridization, to clarify the role of TIMPs in normal and aberrant wound repair. Expression of TIMP-1 and -3 mRNAs was found in proliferating keratinocytes in 3- to 5-day-old normally healing wounds, whereas no epidermal expression was detected in chronic ulcers. However, TIMP-3 protein was found in the proliferating epidermis in 20 of 24 samples representing both full-thickness acute and chronic wounds. TIMP-1 and TIMP-3 also were abundantly expressed by spindle-shaped, fibroblast-like, and plump, macrophage-like stromal cells, as well as by endothelial cells. In normally healing wounds, TIMP-2 protein localized under the migrating epithelial tip and to the stromal tissue under the eschar more frequently than in chronic ulcers. Occasional staining for TIMP-4 protein was detected in stromal cells of chronic ulcers near blood vessels. Our results indicate that TIMP-1 and TIMP-3 may be involved both in the regeneration of the epidermis by stabilizing the basement membrane zone and in the regulation of stromal remodeling and angiogenesis of the wound bed. Lack of TIMP-2 near the migrating epithelial wound edges might contribute to uncontrolled activity of MMP-2 in chronic ulcers. We conclude also that TIMPs are temporally and spatially tightly regulated and that the imbalance between metalloproteinases and TIMPs-1, -2, and -3 may lead to delayed wound healing.     

Adenosine A<Subscript>2A</Subscript> Receptor Blockade or Deletion Diminishes Fibrocyte Accumulation in the Skin in a Murine Model of Scleroderma,Bleomycin-induced Fibrosis

Peripheral blood fibrocytes are a newly identified circulating leukocyte subpopulation that migrates into injured tissue where it may display fibroblast-like properties and participate in wound healing and fibrosis of skin and other organs. Previous studies in our lab demonstrated that A_2A receptor-deficient and A_2A antagonist-treated mice were protected from developing bleomycin-induced dermal fibrosis, thus the aim of this study was to determine whether the adenosine A_2A receptor regulates recruitment of fibrocytes to the dermis in this bleomycin-induced model of dermal fibrosis. Sections of skin from normal mice and bleomycin-treated wild type, A_2A knockout and A_2A antagonist-treated mice were stained for Procollagen α2 Type I and CD34 and the double stained cells, fibrocytes, were counted in the tissue sections. There were more fibrocytes in the dermis of bleomycin-treated mice than normal mice and the increase was abrogated by deletion or blockade of adenosine A_2A receptors. Because fibrocytes play a central role in tissue fibrosis these results suggest that diminished adenosine A_2A receptor-mediated recruitment of fibrocytes into tissue may play a role in the pathogenesis of fibrosing diseases of the skin. Moreover, these results provide further evidence that adenosine A_2A receptors may represent a new target for the treatment of such fibrosing diseases as scleroderma or nephrogenic fibrosing dermopathy.     

损伤皮肤修复和伤口愈合过程中的干细胞：问题与前景

BACKGROUND:Present treatments for chronic skin wounds have certain limitations, and adult stem cells play a potential part in cutaneous repair and regeneration. OBJECTIVE:To review effects of stem cells in skin regeneration and wound healing. METHODS:The first author retrieved CNKI and Medline databases by computer for relevant articles published from 2000 to 2010. The keywords were “epidermal stem cells, hair follicle stem cells, stem cells, transplantation, dermal stem cells” in Chinese and in English, respectively. Then totally 489 papers were obtained after initial survey, and according to the inclusion criteria, 30 articles were selected for review. RESULTS AND CONCLUSION:Epidermal stem cells and other adult stem cells have been applied to treat wounds and other skin diseases. Epidermal stem cells are the crucial cell source of skin development, repair and remodeling. Epidermal stem cells are always in a resting state in vivo. Unless, skin injure or culture in vitro, cell division and proliferation will be significantly fastened. The stability of the epidermis mainly depends on the asymmetric division of a subpopulation, in which two daughter cells are produced, including one with characteristics of stem cells, and the other differentiated into transient amplifying cells that will be differentiated into post mitotic cells after a series of cell divisions (3-5 times). Afterwards, those post mitotic cells are developed into terminal differentiation cells on the basal layer, finally detach from the epidermis as dander. In addition, it is unclear whether epidermal factors are related to apoptosis, migration and differentiation in the process of wound repair and even under physiological conditions. Therefore, application of stem cells in wound healing requires a further discussion.     

Fibrosin: a novel lymphokine in wound healing.

Several growth factors are actively synthesized during wound repair and function to stimulate different cell types involved in the process of healing. Fibrosin is a novel fibrogenic lymphokine that stimulates several biological activities that relate to in vivo scarring. To investigate the role of fibrosin, we used "punch biopsy" and linear wounding procedures in a murine model of wound healing. Histological examination showed that recombinant fibrosin stimulated epithelialization of wounds and accelerated healing of both punch biopsy and linear wounds. Fibrosin enhanced healing of linear wounds by reducing the time for healing by approximately 30-40%. From our data we estimated the healing time of control wounds to be 22-24 days; wounds treated with fibrosin appeared to heal in 14-16 days. Our observations suggest that fibrosin enhances wound healing and may be involved in accelerating epithelialization, collagen matrix formation, and also remodeling of the extracellular matrix in vivo. Thus fibrosin may function during different phases of wound healing and act as a potent inducer of scar formation and wound healing. This finding may have direct clinical applications.     

负压封闭引流技术对大鼠急性创面愈合过程中Ⅰ/Ⅲ型胶原比例变化的影响

文题释义：负压封闭引流技术：是一种用来处理各种急慢性复杂伤口的技术。这种技术主要通过亲水材料聚乙烯醇和疏水材料聚氨酯以及引流管来完成,安全无毒的聚乙烯醇覆盖在皮肤缺损或者软组织损伤部位,在它上面覆盖一层疏水材料聚氨酯,两者之间置入引流管后连接负压吸引装置,设置负压值,医用贴膜封闭由此形成密闭空间,促进创面愈合。 Ⅰ/Ⅲ型胶原比例：皮肤组织中最丰富的物质是胶原蛋白,而Ⅰ/Ⅲ型胶原的量占总胶原的90%以上,Ⅰ型胶原纤维较粗壮,呈条束状,决定着皮层的抗拉伸强度;Ⅲ型胶原纤维较细微,呈疏松网状结构,决定着皮肤的韧性及弹性,所以Ⅰ/Ⅲ型胶原比例决定着皮肤的愈合质量。背景：负压封闭引流技术可以促进各种急慢性创面的愈合。Ⅰ/Ⅲ型胶原比例在维持皮肤组织稳态及皮肤损伤后的修复过程中发挥着重要的作用,但是在负压封闭引流技术促进创面愈合过程中Ⅰ/Ⅲ型胶原比例的变化知之甚少。 目的：观察负压封闭引流技术促进创面愈合过程中Ⅰ/Ⅲ型胶原的比例变化,探讨其对大鼠急性创面的修复机制。方法：建立大鼠背部皮肤全层缺损模型,创面直径20 mm,建立创面模型以后随机分为负压封闭引流治疗组和空白对照组。创面愈合过程中于术后第1,3,7天对创面进行固定高度连续拍照,进而计算和比较创面愈合率;实时荧光定量PCR检测Ⅰ,Ⅲ型胶原mRNA的表达;免疫组织化学染色分析Ⅰ/Ⅲ型胶原比例;组织学检测创面组织肉芽组织再生和再上皮化长度的变化。结果与结论：①与空白对照组相比,负压封闭引流治疗组提高了Ⅰ,Ⅲ型胶原的mRNA和蛋白表达水平(P < 0.05);②术后第3天开始,负压封闭引流治疗组的Ⅰ/Ⅲ型胶原比例高于空白对照组(P < 0.05);③负压封闭引流治疗组的创面愈合率均高于空白对照组(P < 0.05);④上述结果证实,负压封闭引流技术可能通过提高Ⅰ,Ⅲ型胶原蛋白表达和Ⅰ/Ⅲ型胶原比例,增加创面的抗拉伸强度,促进创面的早期愈合。ORCID: 0000-0003-2399-6202(赵鑫) 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程