流行性出血热病人尸体肾脏中IgE型免疫复合物沉积的观察

本文应用免疫酶组化法检测EHF尸体肾脏IgE型免疫复合物,并检测同一肾检病人血清中IgE及其复合物。结果发现,EHF病人肾组织中有IgE型免疫复合物的沉积,通过抑制试验证明复合物为IgE型。病人血清中亦检出较高水平的IgE型复合物。这说明IgE型复合物可随血流沉积于肾脏参与EHF中晚期发病过程。     

IgE及其介导的Ⅰ型变态反应对免疫复合物在肾脏沉积的影响

本文采用豚鼠血清过敏症的动物模型,以热凝聚IgG(HAG)代替免疫复合物(IC),用直接免疫荧光技术,测检了IC在豚鼠肾脏的沉积情况。结果表明,在血清IgE水平增高的豚鼠和发生血清过敏症的豚鼠的肾脏中,IC的沉积量明显高于对照组。这说明,血清IgG水平增高及其介导的Ⅰ型变态反应均可促进IC在肾脏的沉积。从而提示,IgE及其介导的Ⅰ型变态反应可能参与某些免疫复合物性疾病的发病过程。     

流行性出血热病人特异性循环免疫复合物的检测

应用ＥＬＩＳＡ（双抗体夹心法）检测了３３例４～７病日流行性出血热（ＥＨＦ）病人血清中特异性循环免疫复合物（ＣＩＣ）水平；并比较了联合抗过敏加输新鲜血浆疗法治疗前后的血清特异性ＣＩＣ消长情况。结果表明，ＥＨＦ病人可检出ＥＨＦ病毒抗原的特异性ＩｇＥ型和ＩｇＧ型ＣＩＣ。联合抗过敏加输新鲜血浆治疗后的病人血清中特异性ＩｇＥ型和ＩｇＧ型ＣＩＣ均明显降低。     

用ELISA检测流行性出血热特异性免疫复合物的初步研究

为检测流行性出血热特异性循环免疫复合物,建立了抗原特异性双抗体夹心ELISA技术。以兔抗EHF IgG包被反应板,使待检标本内循环免疫复合物通过暴露的EHF病毒抗原位点而被结合到固相上,再以辣根过氧化物酶标记羊抗人IgG进行示踪,从而检出EHF’特异性循环免疫复合物。在228份EHF患者血清中,用胶因素ELISA检出非特异性循环免疫复合物183份,抗原特异性ELISA检出特异性循环免疫复合物188份。本方法特异性强,重复性及稳定性较好,可应用于临床检测。     

卵白过敏反应—抗原特异性IgE免疫复合物的检测

过敏性皮炎(AD)循环血中出现IgE免疫复合物(IgE-IC),其意义尚未明确。婴幼儿AD病人常常合并卵白过敏。本文应用生物素-亲和素ELISA测定法检测患者血清中卵白特异性IgE-IC。     

乙型肝炎病毒(HBV)与肾炎关系的研究——应用单克隆抗体、ABC法检测肾组织中的HBsAg

作者应用抗HBsAg单克隆抗体,亲和素-生物素复合物(ABC)技术,对20例肾炎、PAP酶标法证实组织中有HBV沉积的病人进行了免疫病理研究,以期澄清HBV与肾炎的关系。 研究结果显示,伴HBV血症及非HBV血症的两组病人,ABC法检测结果完全相同。每组中各有1例病人肾脏中HBsAg阴性,其余病例全部呈阳性结果。HBsAg在肾脏中的分布特点与肾炎的组织学类型、     

甲型肝炎患者循环免疫复合物的检测及其临床意义

本文对20例甲肝病人血清用聚乙二醇组分分析法进行了循环免疫复合物(CIC)的检测,发现:(1)甲肝急性期IgM抗体和IgM型CIC明显增高,且IgM型CIC与抗HAV-IgM效价存在着明显的正相关,间接提示抗体IgM以甲肝病毒作为抗原形成CIC(循环免疫复合物),参与病毒的排除。(2)病人血清中CIC水平变化与病情有一定的关系。     

流行性出血热中晚期病人免疫疗法

本文报导了流行性出血热(EHF)中晚期病人血清中循环免疫复合物(CIC)水平上升,补体介导的免疫复合物溶解活性(CRA)显著地低于正常人(P<0.01).应用联合抗过敏疗法加用新鲜血浆进行治疗,获得了良好的疗效.病人血清 CRA 增强,CIC 浓度下降,临床各项指标及肾功能指标明显改善,病死率下降.     

慢性进展型多发性硬化症病人脑脊液和血清中免疫复合物和第3、第4补体因子

实验证明,多发性硬化症(MS)病人血清和脑脊液(CSF)中存在免疫复合物(IC),作为炎症反应的媒介,补体系统在免疫复合物疾病中起主要作用。本文介绍了慢性进展型MS病人血清、CSF样品中IC和第3、第4补体因子(C_3、C_4)水平,及其活性产物(AP)的检测情况。本实验配对检测32例慢性进展型MS病     

系统性红斑狼疮病人的IgE水平

IgE为Ⅰ型变态反应的反应素,参与支气管哮喘和特应性皮炎的发展。实验研究证明,含IgE 的免疫复合物沉积在血管壁促进血管炎的发展。作者研究系统性红斑狼疮(SLE)病人的IgE 水平,检查SLE67例,女56例,男11例,年龄15～73岁,平均32.8±11.9岁。高度或中度活动性SLE46例,有变态反应32例(43%),常有药物性荨麻疹;有血管神经性水肿4例,变应性结膜炎1例,食物过敏1例。对照组为30例健康人。用放射免疫法测定血清IgE 浓度(用免疫试剂pharmacia)正常上界为100KU/L。用酶联免疫吸附法测定抗心磷脂抗体。检查结果:SLE 病人血清IgE 波动范围很大,为2～1132KU/L,平均158±240KU/L。IgE>100KU/L 组23例(34%),多有毛细血管炎,网状青斑,下肢营养性溃疡,“鳞翅目”,粘膜疹和亚急性皮肤红斑狼疮。     

流行性出血热病人IgE与IgE－CIC产生动态及影响CIC沉积和排除的因素

应用双抗体夹心法和ＰＥＧ沉淀－ＥＬＩＳＡ双抗体夹心法分别检测了ＥＨＦ病人血清中ＩｇＥ和ＩｇＥ－ＣＩＣ产生的动态水平。检测结果发现ＩｇＥ水平于发病３～５天达高峰ＩｇＥ－ＣＩＣ于发病６～１０天达高峰，此时机体ＲＢＣ排除ＣＩＣ及补体溶解ＩＣ活性（ＣＲＡ）功能低下，随着排除ＣＣ功能的恢复，ＣＩＣ减少。如往病人血清中加入含补体血清时，ＣＩＣ被溶解，ＣＩＣ水平下降，提示给病人输注大量新鲜含补体血浆可达治疗的目的。     

Monocyte-dependent serum suppression of lymphocyte blastogenesis in Hodgkin's disease: An association with nephrotic syndrome

A factor inhibitory to PHA-induced lymphocyte blastogenesis was found to be present in the serum of a patient with advanced Hodgkin's disease and nephrotic syndrome. The inhibitory activity for both syngeneic and allogeneic lymphocytes was dependent on the presence of peripheral blood monocytes. The Raji-cell serum assay, as well as immunofluorescence and light and electron microscopy of the renal biopsy, showed no evidence of immune complexes. Nevertheless, a high serum IgE level as well as the finding that ultracentrifugation and heating at 56°C significantly reduced the inhibitory activity (P < 0.01) suggested the possibility that an immune complex might have mediated the suppressive activity. Treatment of the Hodgkin's disease with combined chemotherapy caused a marked reduction in the monocyte-dependent serum inhibitory activity which in turn coincided with a prompt remission of the nephrotic syndrome and marked regression of disease.     

Serum concentrations of immunoglobulins E and G and α2-macroglobulin in childhood renal disease

Serum concentrations of IgE, IgG, and α₂-macroglobulin (α₂M) were determined in 67 children with renal disease and in appropriate controls. IgE concentrations were significantly elevated in patients with renal disease and proteinuria, including those with minimal change nephrotic syndrome (MCNS). IgG concentrations were lower and α₂M concentrations higher in patients with proteinuria than in patients without proteinuria. In 16 patients with nephrotic syndrome who had a decrease in proteinuria with therapy, there was a concomitant decrease in mean serum IgE level. All nine with MCNS had a decrease in serum IgE following therapy whereas eight of the nine had a concomitant increase in serum IgG. The findings suggest the possibility that IgE may play an etiologic role in the proteinuria of childhood nephrotic syndrome, and that both elevated serum IgE and proteinuria may represent unusual responses to antigens that are seldom identified. Elevations of IgE secondary to lowered IgG or to entirely nonspecific alterations in IgE metabolism in proteinuric renal disease are other possibilities. Further studies to elucidate the role of IgE in renal disease, including the nature of the altered metabolism of this immunoglobulin, are warranted.     

Differences in the changes of allergen-specific IgE serum levels and the chemiluminescence of peripheral blood phagocytes in patients with allergic rhinoconjunctivitis during the ragweed season

The objective of this study was to compare the changes in the values of allergen-specific serum IgE levels and zymosan-induced whole blood chemiluminescence (CL) in 41 patients who had exclusively only ragweed allergy in the season of acute symptoms of disease in July, August and September. All patients had allergic rhinitis or rhinoconjunctivitis. Each patient was investigated as a self-control. The ragweed-specific IgE levels were measured by enzyme immunoassay (EIA). The luminol amplified zymosan-induced CL of whole human blood was detected. The allergen-specific serum IgE levels showed slight, but not significant, gradually increasing elevations during the whole season. On the other hand, significant increases were found in the values of the basal but especially in the zymosan-stimulated CL of peripheral blood phagocytes during the acute phase of allergy. Both the basal and the zymosan-induced CL reflected significantly the activated state of the immune system. These observations clearly show that there are well detectable signs of the systemic activation of the immune system in allergic rhinoconjunctivitis beside the local alterations. In addition, the measurements of the basal and zymosan-induced CL of peripheral phagocytes could clearly reflect the clinical state of disease in vitro.     

Total Serum IgE Levels in Normals and Patients with Chronic Non–Specific Lung Diseases

Total serum IgE was measured, using a sensitive sandwich method, in a healthy control population, in healthy persons with a familial history of chronic non-specific lung disease and in patients with chronic non-specific lung disease (CNSLD). In the control population a median total serum IgE of 39 1.U./ml was found. Total serum IgE levels were significantly lower in women that in men. Healthy persons with a familial history of CNSLD had significantly increased total IgE levels compared with the control population. The IgE level was even higher in the group of patients with CNSLD The presence of specific IgE antibodies and/or an early onset of the symptoms of CNSLD was accompanied by higher total serum IgE levels compared with the IgE values in the other CNSLD patients. But the total IgE levels were increased in all subgroups of CNSLD patients. A significant decrease of the IgE level with age was observed in CNSLD patients.