十味平痤胶囊对兔耳痤疮模型抗角化作用的研究

目的建立兔耳痤疮模型,观察十味平痤胶囊的抗粉刺作用。方法采用Kligman法建立兔耳痤疮模型,以涂橄榄油作对照。造模成功后将家兔随机分为大、中、小剂量组、阳性对照组和阴性对照组,分别灌饲十味平痤胶囊、三蕊胶囊和生理盐水2周,在造模前后及灌胃前后分别切取组织于光镜、电镜下观察结果。结果外涂煤焦油2周后,兔耳表面粗糙,可见粉刺、丘疹,且光镜、电镜下组织学改变与人类痤疮相似;涂橄榄油处肉眼及光镜下改变不大。灌药2周后,实验各剂量组和三蕊胶囊组肉眼可见粉刺、丘疹消退;光镜及电镜下亦有相应改变。结论Kligman法造模稳定可靠、重复性强;十味平痤胶囊可纠正兔耳毛囊上皮的异常角化。     

复方紫金霜对兔耳粉刺模型治疗及P物质的影响

目的建立兔耳粉刺模型,观察复方紫金霜对实验性粉刺的治疗作用和对P物质(SP)的影响,以探讨其治疗机制。方法将2%煤焦油溶液外用于家兔左耳内侧,连续2周。2周后将家兔分为3组,分别在兔左耳内侧外涂复方紫金霜、5%硫磺粉刺洗剂和生理盐水治疗3周。同时,采用免疫组化SABC法检测治疗前后兔耳模型皮损中的SP。结果外涂煤焦油2周后,肉眼可见兔耳出现粉刺,在普通光镜下呈现与人类痤疮类似的病理改变;免疫组化染色可见SP呈阳性反应。外用药3周后紫金霜组兔耳粉刺大部分消退,基本接近正常对照兔,SP呈弱阳性或阴性;硫磺粉刺组粉刺部分减少,SP呈阳性或弱阳性;生理盐水组无明显变化,SP呈阳性。结论复方紫金霜对实验性粉刺治疗效果较好,可明显减少兔耳粉刺模型的毛囊角栓,减轻炎症反应,降低SP的表达。     

清肺饮颗粒对家兔实验性耳痤疮影响的研究

目的建立家兔实验性耳痤疮模型,观察复方清肺饮颗粒治疗痤疮的作用。方法家兔左耳内侧涂浓煤焦油溶液0.5ml,1次/d,连续2周,建立耳痤疮动物模型。耳痤疮模型建立成功后将家兔随机分为清肺饮颗粒大、中、小剂量组、解毒痤疮丸组、模型实验组、正常对照组,分别灌饲清肺饮颗粒、解毒痤疮丸和蒸馏水2周,在造模前后及药物治疗前后,取家兔左右耳廓相同部位耳片,称重后计算耳片重量差值,光镜下观察耳廓组织病变。结果外涂煤焦油1周后,兔耳表面粗糙,增厚,可见粉刺、丘疹,组织学改变与人类痤疮相似;给药2周后,清肺饮颗粒不同剂量组和解毒痤疮丸组均可不同程度抑制痤疮模型家兔耳重量的增加,并明显改善耳痤疮炎性病理变化。结论清肺饮颗粒对家兔实验性耳痤疮有治疗作用,可用于痤疮的防治。     

中药痤疮冲剂对兔耳实验模型抗角化作用的研究

目的 建立痤疮实验动物模型,并用来观察中药的抗粉刺作用.方法 采用Kligman法煤焦油外涂于白色家兔耳管开口处,每日1次,连续2周,然后将白色家兔分为两组,第1组灌饲痤疮冲剂2周,第2组不做任何处理.结果 外涂煤焦油2周后,肉眼可见兔耳呈粉刺样发疹,光镜、电镜下观察其改变与人类粉刺相似.又经过 2周后,第1组粉刺样改变明显减轻,第2组未见明显改变.结论 兔耳粉刺实验动物模型具可重复性,并能用于观察药效;中药痤疮冲剂能减少粉刺动物模型的毛囊角栓和减轻真皮炎症.     

姜黄消痤凝胶对兔耳粉刺模型的抗角化作用

目的采用煤焦油制造实验兔耳粉刺模型,观察姜黄消痤凝胶(AGC)对动物粉刺模型的抗角化作用。方法将30只雄性大耳白兔随机分为模型组、基质组、AGC低剂量组、AGC高剂量组和阳性对照组。造模成功后,模型组兔右外耳道不外搽任何药物作为模型对照,后四组分别在右外耳道每日外用空白凝胶基质1g,AGC1g,AGC2g和维A酸乳膏1g,连用3周后,肉眼和组织学观察AGC抗粉刺作用。结果外涂煤焦油2周后,兔耳表面粗糙、增厚,可见粉刺,组织学改变与人类粉刺相似;给药2周后AGC组和阳性组肉眼可见兔耳变薄、变软,粉刺减少或消退,组织学亦有相应变化。结论 AGC对实验性粉刺有缩小毛囊面积、皮脂腺直径、减轻表皮增厚和减轻真皮炎症细胞浸润等作用。     

加味枇杷清肺颗粒对兔耳痤疮模型抗角化作用及睾酮水平的影响

目的观察加味枇杷清肺颗粒对兔耳痤疮模型的治疗效果。方法用5%煤焦油溶液涂抹兔耳造模,成功后随机分组,分别灌胃加味枇杷清肺颗粒、枇杷清肺饮经典方颗粒和珍珠暗疮片2周。通过肉眼和镜下观察抗角化作用,采用放射免疫分析法测定血清睾酮含量。结果造模后,肉眼可见兔耳表面粗糙、增厚、有粉刺样皮损,组织病理改变与人类痤疮相似。灌胃加味枇杷清肺颗粒后,兔耳毛囊角化程度改善,血清睾酮含量降低,并呈量效关系。结论加味枇杷清肺颗粒有抗实验性痤疮的作用。     

痤疮消霜对兔耳痤疮模型的作用研究

目的:观察痤疮消霜对兔耳痤疮模型的影响。方法:选取新西兰兔30只,雌雄不限,分为正常对照组、模型组、痤疮消霜高剂量组、痤疮消霜低剂量组、维A酸霜组共5组。正常对照组3只不涂煤焦油,不用药;余下27只分别外涂煤焦油造模2周,并于造模成功后每天外涂乳膏基质、痤疮消霜(高、低剂量)、0.1%维A霜3周。于末次给药后24小时,以打孔器制作涂药处耳片标本,以10%甲醛溶液固定,苏木精-伊红染色,光学显微镜下观察组织病理学改变,并进行显微照相。结果:27只新西兰兔造模成功24只,未造模成功的3只归为正常对照组。经观察,在改善兔耳痤疮反应强度上,痤疮消霜高剂量组与模型组比较(t=2.62,P<0.05),说明痤疮消霜高剂量能明显降低兔耳痤疮反应强度,有很好的治疗痤疮的作用;痤疮消霜高剂量组与维A酸霜组相比较(t=0.06,P>0.05),两者无统计学差异。同时,在兔耳病理改变上,痤疮消霜高剂量组兔耳病变改善明显,其表皮增厚状况明显减轻,毛囊口扩张程度减轻,毛囊四周角化层明显减轻,炎性细胞减少,与模型组相比(χ~2=4.00,P<0.05),有统计学差异。痤疮消霜低剂量组兔耳病变程度与模型组相比(χ~2=1.09,P>0.05),无统计学差异,未见明显改善。维A酸霜组与痤疮消霜高剂量组相比(χ~2=0.34,P>0.05),无统计学差异。结论:痤疮消霜高剂量组对煤焦油所致兔耳痤疮模型有明显改善作用。     

茶多酚乳膏对痤疮动物模型的实验研究

目的：研究茶多酚（tea polyphenols，TP）乳膏外用对实验性痤疮动物模型的治疗效果。方法：采用煤焦油外涂致兔耳实验性角化和金黄地鼠皮脂腺斑增生模型，于动物模型的病变部位外搽一定剂量的12％、6％和3％TP乳膏，结合组织病理学检查和其他量化指标观察TP乳膏的抗痤疮作用。结果：家兔耳郭涂煤焦油2周后，肉眼可见兔耳皮肤粗糙、增厚、有粉刺样皮损，组织病理检查示其病变与人类粉刺相似。TP乳膏涂搽后可明显减少毛囊角化物，减轻皮肤增厚；金黄地鼠皮脂腺斑涂搽TP乳膏后，能明显减轻皮脂腺斑增厚，并使其变得柔软，表明TP乳膏对皮脂腺增生有抑制作用。结论：TP乳膏可抑制实验性毛囊上皮角化过度和皮脂腺增生，表明该制剂具有抗实验性痤疮的作用。     

皮脂腺及皮下脂肪疾病

复方紫金霜对兔耳粉刺模型治疗及P物质的影响,阿维A治疗囊肿性痤疮33例疗效观察,阿达帕林凝胶联合米诺环素治疗寻常性痤疮疗效观察,不同剂量维胺脂胶囊联合0．1％阿达帕林凝胶治疗寻常痤疮113例,洛美沙星、西咪替丁联合对中重度痤疮的疗效观察.     

维胺酯胶囊和盐酸米诺环素治疗囊肿性痤疮临床疗效观察

为观察维胺酯胶囊和盐酸米诺环素治疗囊肿性痤疮的疗效，将囊肿性痤疮患者随机分成3组，A组口服维胺酯胶囊和盐酸米诺环素；B组口服维胺酯胶囊；C组口服盐酸米诺环素。均治疗8周。A组对粉刺、丘疹和脓疱的疗效优于C组（P〈0．05），对结节和囊肿的疗效优于B组（P〈0．05）；综合疗效A组优于B组和C组（P〈0.05）：维胺酯胶囊和盐酸米诺环素联合治疗囊肿性痤疮较两药单独治疗可明显提高疗效。     

Enhancement of comedogenic substances by ultraviolet radiation

Ultraviolet radiation enhanced the capacity of human sebum, sulphur, cocoa butter, squalene, and coal tar to produce comedones in the external ear canals of rabbits. An enhancement of the comedogenicity of coal tar and squalene was similarly demonstrated in man. We conjecture that in occasional patients sunbathing may aggravate acne by augmenting the comedogenicity of sebum.     

龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的影响

目的 探讨龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的作用效果.方法 24只新西兰家兔随机分为空白组4只和模型组20只,模型组以煤焦油涂抹和痤疮丙酸杆菌接种制备兔耳痤疮模型后,随机分为模型组、阳性药组、龙血竭凝胶高、中、低剂量组[根据对痤疮丙酸杆菌(P.acne)、金黄色葡萄球菌(S.aureus)、表皮...     

Dark chocolate exacerbates acne

The effects of chocolate on acne exacerbations have recently been reevaluated. For so many years, it was thought that it had no role in worsening acne. To investigate whether 99% dark chocolate, when consumed in regular daily amounts, would cause acne to worsen in acne‐prone male subjects, twenty‐five acne prone male subjects were asked to consume 25 g of 99% dark chocolate daily for 4 weeks. Assessments which included Leeds revised acne scores as well as lesion counts took place weekly. Food frequency questionnaire was used, and daily activities were recorded. Statistically significant changes of acne scores and numbers of comedones and inflammatory papules were detected as early as 2 weeks into the study. At 4 weeks, the changes remained statistically significant compared to baseline. Dark chocolate when consumed in normal amounts for 4 weeks can exacerbate acne in male subjects with acne‐prone skin.     

Effect of isotretinoin on experimentally induced comedones

Summary The effect of isotretinoin on experimentally induced comedones was studied by means of histoplanimetry and scanning electron microscopy (SEM). To induce comedone formation, insoluble cutting oil was applied to the ventral surface of the ears of rabbits for 2 weeks. After comedones formed, isotretinoin was fed to the rabbits by a feeding tube, daily, for 4 weeks. A low dose (2.0 mg/kg) and a high dose (20.0 mg/kg body weight) were selected for two different groups. Soybean oil served as the vehicle. Histoplanimetrically, the high-dose group showed a significant decrease in size of comedones when compared to the control group and the low-dose group [P<0.005], and in the high-dose group, the follicular lumen contained a small number of loose, non-adherent, horny cells. Upon SEM examination, the comedones were seen to have a chrysanthemum appearance before treatment. After treatment, the high-and low-dose groups both showed evidence of the elimination of comedones on three-dimensional analysis.