CD34+细胞水平变化对老年人不同程度慢性左心衰竭的影响

目的 观察循环CD34+细胞(CD34+)水平变化对不同程度老年慢性左心衰竭的影响.方法 根据美国纽约心脏病学会(NYHA)分级临床标准和左室射血分数(LVEF)将所有入选者分为Ⅰ级组23例,Ⅱ级组27例,Ⅲ级组20例,Ⅳ级组16例,同期健康对照组41例.测定不同程度老年慢性左心衰竭患者CD34+的水平,并与肿瘤坏死因子-α(TNF-α)、可溶性肿瘤坏死因子受体1、2(sTNFR-1 sTNFR一2)和血管内皮生长因子(VEGF)水平进行对照分析.结果 慢性左心衰竭的早期CD34+水平升高,随着慢性左心衰竭程度的加重CD34+的水平下降,对照组、NYHA I组、NYHAⅡ组、NYHAⅢ组和NYHAⅣ组分别为(0.6±0.2)×108/L、(2.4±0.4)×109/L、(1.9±0.2)×109/L、(1.3±0.1)×109/L和(0.5±0.2)×109/L,两两比较差异有统计学意义(均P<0.01).TNF-α,、sTNFR-1、sTNFR-2和VEGF水平明显增高,NYHA Ⅰ组与NYHAⅣ组TNF-α[分别为(28.4±10.8)ng/L与(61.4±15.7)ng/L]、sTNFR-1[(690.8±62.7)ng/L与(2820.9±1282.8)ng/L]、sTNFR_2[(740.8±112.3)ng/L与(4113.1±1102.2)ng/L]、VEGF [(423.3±147.9)ng/L与(996.3±487.1)ng/L]比较,差异有统计学意义(均P<0.01).结论 CD34+水平的变化可能预测老年慢性左心衰竭的发生、发展及严重程度.     

冠心病患者Toll样受体4与冠脉病变严重程度的相关性

目的 探讨冠心病患者Toll样受体（toll like receptors,TLR）4水平与冠状动脉（冠脉）病变严重程度的相关性。方法 选取我科住院经冠状动脉造影确诊为冠心病的患者141例。采用Syntax评分反映冠脉病变严重程度,根据Syntax评分结果分为低分(0~22分)61例、中分(23~32分)41例和高分(≥33分)39例。据此分为2组:Syntax低中分组(n=102),Syntax高分组(n=39)。通过线性相关分析确定TLR4水平与冠脉病变严重程度的关系,通过多因素Logistic回归分析确定冠脉病变的相关因素。结果 与低中分组比较,高分组TLR4水平明显升高〔(93±24) vs.(128±29) ng/ml,P<0.01〕。TLR4水平与Syntax评分呈正相关(r=0.539,P<0.01)。多因素回归分析显示,TLR4水平是Syntax评分≥33分的相关因素(OR=15.645,95%CI:5.322-45.994;P<0.01）。结论 TLR4与冠脉病变严重程度密切相关,随着TLR4水平升高,冠脉病变越严重。     

循环内皮细胞微颗粒和高敏C反应蛋白水平变化与慢性左心衰竭严重程度的相关性研究

目的 探讨循环内皮细胞微颗粒(EMP62E、EMP31)和高敏C反应蛋白(hs-CRP)水平变化与慢性左心衰竭严重程度的相关性.方法 根据美国纽约心脏病学会(NYHA)心功能分级和左室射血分数(LVEF)将健康对照者和慢性左心衰竭患者分为对照组[LVEF(6.97±4.65)%]、Ⅰ级组[LVEF(42.67±2.06)%]、Ⅱ级组[LVEF(34.26±3.17)%]、Ⅲ级组[LVEF(29.05±1.07)%]和Ⅳ级组[LVEF(25.17±1.42)%].采用流式细胞仪检测健康对照者和慢性左心衰竭患者EMP62E、EMP31的表达.结果 随着NYHA心功能分级的升高和LVEF的下降,EMP62E、EMP31和hs-CRP的水平明显上升.Ⅳ级组和Ⅰ级组比较,EMP62E(1092.7±102.8)和(291.0±21.9)个/μl、EMP31(1596.1±46.3)和(477.8±40.3)个/μl和hs-CRP(14.7±0.1)和(4.9±0.1)mg/L,差异有统计学意义(均P＜0.01).结论 EMP62E、EMP31和hs-CRP的水平显著升高可能参与了慢性左心衰发生发展的机制.

Abstract：

Objective To study the relationship of levels of circulating endothelial microparticles (EMP62E, EMP31) and high-sensitivity C-reactive protein (hs-CRP) with severity of chronic left heart failure in elderly patients. Methods According to New York Heart Association (NYHA) class and left ventricular ejection fraction (LVEF), the healthy subjects and the patients were divided into five groups: control group [LVEF: (63.97±4.65)%], classⅠ group [LVEF: (42.67±2.06)%], classⅡ group [LVEF: (34.26±3.17)%], class Ⅲ group [LVEF: (29.05±1.07)%] and class Ⅳ group[ LVEF:(25.17±1.42)%] . The levels of circulating EMP62E, EMP31 and hs-CRP of the patients and healthy subjects were measured by flow cytometry and nephelometry immunoassay, respectively. Results There were significantly differences in EMP62E, EMP31 and hs-CRP between class Ⅳ group and classⅠ group P＜0.01) EMP62E [(1092.7 ± 102.8) counts/μl vs. (291.0±21.9) counts/μl], EMP31 [(1596.1±46.3) counts/μl vs. (477.8±40.3) counts/μl] and hs-CRP [(14.74±0.07) mg/L vs. (4.86 ± 0.09) mg/L]. The levels of circulating EMP62E, EMP31 and hs-CRP were gradually elevated significantly along with the increased severity of chronic left heart failure in elderly. Conclusions The upregulation of circulating EMP31, EMP62E and hs-CRP may contribute to the development of chronic heart failure in elderly.     

Toll样受体、CD4+CD25+调节性T细胞与支气管哮喘的关系

Toll样受体（TLR）是机体识别环境中各种微生物的主要受体，是介导天然免疫和获得性免疫的桥梁。支气管哮喘（哮喘）的发病是环境和基因相互作用的结果，TLR与哮喘有着十分密切的关系。近年来，人们逐渐认识到Th1／Th2理论并不能充分阐明哮喘的发病机制。随着对哮喘的深入研究，CD4^＋CD25^＋调节性T细胞凸现其冰山一角。本文就近年来对TLR、CD4^＋CD25^＋调节性T细胞和哮喘关系的研究进展作一综述。     

CD14及Toll样受体4基因多态性与大肠癌的相关性研究

目的探讨我国湖北汉族人Toll样受体（TLR）4基因Asp299Gly和CD14 C-260T基因多态性分布与大肠癌的相关性。方法采用聚合酶链反应限制性片段长度多态性（PCR—RFLP）方法，检测110例大肠癌患者及160例正常对照者TLR4基因Asp299Gly及CD14 C-260T基因型及等位基因频率的分布。结果大肠癌组CD14 C-260T基因型与正常对照组比较，差异有统计学意义（P〈0．05）。正常对照组CC基因型的频率为15．6%，明显低于大肠癌组的31．8％（P=0．0027，OR=0．3968，95％CI=0．2209～0．7129）；正常对照组中CT基因型的频率为48．1％，明显高于大肠癌组的30．9％（P=0．0056，OR=2．074，95%CI=1．246～3．452）。所有样本中均未发现TLR4基因Asp299Gly的突变型。结论CD14 C-260T基因多态性与中国湖北汉族大肠癌显著相关，而TLR4基因Asp299Gly多态性与大肠癌无关。     

Toll样受体4与乙型肝炎的相关性

Toll样受体(TLR)4是一种重要的模式识别受体,在抑制HBV感染中发挥着重要的作用。TLR4识别HBV相关蛋白分子,经髓系分化因子88(My D88)和非My D88途径,产生肿瘤坏死因子α、白细胞介素(IL)6、IL-12等炎症因子和Ⅰ型干扰素而发挥抗病毒作用。同时发现TLR4可通过调节辅助性T淋巴细胞(Th)1与Th2间的平衡,促进CD8+T淋巴细胞在肝脏粘附及诱导一氧化氮合酶发挥抑制HBV作用。若TLR4过表达,会通过机体免疫系统过度激活,加重肝脏的缺血再灌注及肝星状细胞激活而加重肝脏炎症。回顾了国内外近年相关文献,探讨了TLR4在乙型肝炎发病中的相关机制,总结了TLR4与乙型肝炎的相关性,以期通过TLR4与乙型肝炎相关性的研究,发现治疗乙型肝炎及延缓其向肝硬化和肝癌进展的新方案。     

老年人慢性左心力衰竭严重程度与循环内皮细胞微颗粒CD31~+水平变化的关系

目的探讨外周血循环内皮细胞微颗粒CD31~+水平变化与老年人慢性左心力衰竭严重程度的相关性。方法根据纽约心功能分级(NYHA)和左心室射血分数(LVEF)将老年慢性左心力衰竭患者分成Ⅰ级、Ⅱ级、Ⅲ级和Ⅳ级组,并纳入同期健康老年人30名作为对照组。采用流式细胞仪检测健康对照者和老年慢性左心力衰竭患者CD31~+的表达。结果随着NYHA的升高和LVEF的下降,CD31~+的水平明显上升(P<0.01)[对照组(348±42)个/μl;Ⅰ级组(478±40)个/μl;Ⅱ级组(659±42)个/μl;Ⅲ级组(846±43)个/μl;Ⅳ级组(1596±46)个/μl,均为P<0.01]。结论 CD31~+表达显著上调可能参与了老年人慢性左心力衰竭发生发展。     

CD34+细胞和血管内皮功能与慢性阻塞性肺疾病急性期的相关性

目的 观察慢性阻塞性肺疾病急性加重期(AECOPD)患者外周血中CD34+细胞数量的变化,并探讨这些细胞的变化是否与内皮功能和全身炎症的改变有关.方法 比较AECOPD患者、稳定期COPD患者、肺功能正常的吸烟者、健康从不吸烟者4组循环中CD34+细胞的水平.检测血浆血管内皮生长因子(VEGF)和C反应蛋白(CRP)水...     

脑梗死患者外周血CD34+细胞水平与脑血管病危险因素的相关性

目的 观察急性脑梗死患者外周血CD34+细胞水平的变化,探讨其与脑血管病危险因素、神经功能缺损评分及颈动脉内膜-中层厚度(IMT)的相关性.方法 选择急性期脑梗死(发病72 h内)患者45例(梗死组)和具有脑血管危险因素的非脑梗死患者27例(高危组)为研究对象.记录两组患者脑血管病危险因素,包括酗酒史、吸烟史、冠心病、高血压、糖尿病、血清三酰甘油、总胆固醇、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;通过流式细胞仪测定两组患者外周血CD34+细胞水平,再将两组外周血CD34+细胞水平与脑血管病危险因素进行相关性分析;分别记录梗死组神经功能缺损评分及颈动脉IMT,将外周血CD34+细胞水平与神经功能缺损评分及颈动脉IMT进行相关性分析.结果 (1)脑血管病危险因素中冠心病、高血压、糖尿病和LDL-C水平均与外周血CD34+细胞水平呈显著负相关(r分别为-0.749,-0.717,-0.688,-0.764),差异均有统计学意义(P＜0.01);(2)通过多元线性回归分析得出外周血CD34+细胞水平可以作为急性脑梗死的独立影响因素(P＜0.05);(3)梗死组患者外周血CD34+细胞水平低于高危组,其与神经功能缺损评分呈负相关(r=-0.721,P＜0.01),与颈动脉IMT亦呈负相关(r=-0.695,P＜0.01).结论 外周血CD34+细胞水平可以作为急性脑梗死的独立影响因素;急性脑梗死患者外周血CD34+细胞水平与神经功能缺损评分及颈动脉IMT呈显著负相关;外周血CD34+细胞水平可以作为缺血性脑卒中患者早期血管内皮功能的细胞学标志物.

Abstract：

Objective To observe the change of peripheral blood CD34+ cell level in patients with acute cerebral infarction, and explore its relationships with cerebrovascular risk factors,neurological function and carotid artery intima-media thickness (IMT). Methods The 45 patients with acute cerebral infarction (onset within 72 h) (infarction group) and 27 patients with cerebr ovascular risk factors but without cerebral infarction (high-risk group) were chosen for the study. The cerebrovascular disease risk factors including history of alcohol abuse, smoking, coronary heart disease, hypertension, diabetes, abnormal levels of serum triglycerides, total cholesterol,low-density lipoprotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C) were recorded in all subjects. The peripheral blood CD34+ cell levels were measured by flow cytometry.The correlations of peripheral blood CD34+ cell level with cerebrovascular disease risk factors were analyzed. The neurological function and carotid artery IMT were recorded in infarction group, and the correlations of peripheral blood CD34+ cell level with neurological function and carotid artery IMT were analyzed. Results (1) The peripheral blood CD34+ cell level was significantly negatively correlated with coronary heart disease, hypertension, diabetes and LDL-C level (r =- 0. 749,-0. 717, - 0. 688, - 0. 764, all P＜0. 01) ; (2) Multiple linear regression analysis showed that peripheral blood CD34+ cell level was an independent relative factor of acute cerebral infarction (P＜0.05); (3) The peripheral blood CD34+ cell level was lower in infarction group than in high-risk group, and was significantly negatively correlated with neurological deficit score (r=-0. 721, P＜0.01) and carotid artery IMT (r= -0. 695, P＜0. 01). Conclusions Peripheral blood CD34+ cell level could be an independent relative factor of acute cerebral infarction; The peripheral blood CD34+ cell level is significantly negatively correlated with neurological function and carotid artery IMT in patients with acute cerebral infarction; And it can be used as cytological marker which reflect early vascular endothelial function in patients with ischemic stroke.     

高密度脂蛋白胆固醇水平与慢性心力衰竭严重程度的相关性

目的:探讨心力衰竭患者心功能严重程度(NYHA分级)与血清HDL-C水平的相关性。方法:回顾性收集2013年8月至2016年8月,北京安贞医院心内科收治的心力衰竭患者209例(心功能分级NYHA分级Ⅱ~Ⅳ级)为心力衰竭组,根据病因分为缺血性心肌病(ICM)组和扩张性心肌病(DCM)组;其他原因入院的心功能正常的非器质性心脏病患者104例为对照组。比较各组一般资料,血脂及血清脑钠肽(取对数值Lg BNP)是否存在差异;比较ICM组与DCM组NYHA分级III-IV级的严重心力衰竭患者HDL-C,LDL-C水平及心脏超声各指标的差异;相关分析心力衰竭NYHA分级与HDL-C的相关性。结果:心力衰竭组收缩压和BNP显著低于对照组(P0.01);ICM组LDL-C水平高于DCM组和对照组(P0.05),HDL-C水平低于DCM组和对照组(P0.05)。NYHA分级III-IV级心力衰竭患者,DCM组LVEDD大于ICM组,室间隔厚度小于ICM组(P0.01)。ICM组和DCM组心功能III-IV级心力衰竭患者HDL-C水平均低于心功能II级患者(P0.05)。Spearman相关分析显示,HDL-C水平与心力衰竭患者NYHA分级均呈负相关(r=-0.290,P=0.01,r=-0.235,P0.05)。结论:ICM和DCM心力衰竭患者HDL-C水平均有降低,ICM组HDL-C水平更低,且HDL-C水平与ICM和DCM的CHF患者的心力衰竭严重程度均呈负相关。     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.     

脑梗死患者外周血CD34+细胞水平和预后价值

目的 检测首发非腔隙性脑梗死患者体内CD34+细胞水平的变化,探讨外周血CD34+细胞水平的预后价值.方法采用流式细胞仪测定119例非腔隙性脑梗死患者外周血CD34+细胞水平,根据检测值分为高于平均值组(A组)和低于平均值组(B组),比较两组入院时美国国立卫生研究卒中量表(National Institutes ofHealth Stroke Scale,NIHSS)评分和发病3个月时改良Rankin量表(modified Rankin scale,mRS)评分.结果人院时,A组CD34+细胞水平显著高于B组(0.048±0.001对0.032 4±0.002,P<0.05),但NIHSS评分无显著差异(17.51±5.33分对17.64±5.19分).入院1周后,A组CD34+细胞水平增加值显著高于B组(0.001±0.003对～0.005±0.0001,P<0.05).3个月时A组mRS评分显著优于B组(2.98 ±1.14分对3.25±1.39分,P<0.05).相关性研究显示,发病初期CD34+细胞含量与卒中后3个月时mRS评分呈负相关(r=-0.48,P<0.05).结论 外周血CD34+细胞水平较高的急性脑梗死患者预后较好;CD34+细胞水平可作为判断脑梗死患者预后的指标.     

脑梗死患者外周血CD34+细胞水平和预后价值

Objective To detect the alteration of circulating CD34 + cell level in patients with the In'st-ever nonlacunar stroke and to investigate the prognostic value of CD34 +cell level. Methods 1he level of the circulating CD34+ cells were examined using flow eytometry in 119 patients with nonlacunar infarction. 1he patients were divided into group A (higher than average) and group B (lower than average) according to the detected values, The National Institutes of Health Stroke Scale (NIHSS) scores on admission and the modified Rankin Scale (mRS) scores at 3 months after the onset in the two groups wre compared. Results The level of CD34 + cells in group A was significantly higher than that in group B (0. 048 ± 0. 001 versus 0. 032 ± 0. 002, P < 0. 05 ), however, there was no significantly difference in NIHSS sores between the two groups. One week after admission, the increased level of CD34 + cells in group A was significantly higher than that in group B (0. 001 ±0. 003 versus - 0. 005 ± 0. 000, P < 0. 05 ). Three months after the onset of symptoms, the average mRS score in group A was significantly superior to that in group B (2. 98 ± 1.14 versus 3.25 ± 1.39, P <0. 05). 1he level of circulating CD34 + cells at the initial onset of symptom was negatively correlated with the mRS score at 3 months after stroke (r = -0. 48, P <0. 05). Conclusions The higher level of circulating CD34+ cells had better prognosis in patients with cerebral infarction. The level of circulating CD34+ ceils may be used as a prognostic indicator in patients with cerebral infarction.