组织蛋白酶S和组织蛋白酶K在人曲张大隐静脉平滑肌细胞中的表达

目的:探讨组织蛋向酶 S(Cat S)、组织蛋白酶 K(Cat K)在曲张大隐静脉平滑肌细胞(SMC)中的表达变化.方法:术中收集曲张及正常大隐静脉标本,采用免疫组织化学显色方法、免疫印迹法检测 Cat S、Cat K 存曲张和正常大隐静脉管壁的表达.结果:Cat S 和 Cat K 在曲张大隐静脉中免疫反应阳性,阳性细胞主要位于 SMC 胞质,曲张组 SMC 中 Cat S 和 Cat K 阳性细胞平均光密度值较正常组明显增高,其蛋白表达明显高于正常组,免疫荧光显色可见Cat S、Cat K 与曲张大隐静脉 SMC 共定位.结论:Cat S 和 Cat K 在曲张大隐静脉 SMC 中表达明显增高,表明两者可能参与了静脉曲张的发生,并可能作为该疾病的生物学标志.     

组织蛋白酶S和组织蛋白酶K在人曲张大隐静脉平滑肌细胞中韵表达

目的：探讨组织蛋白酶S（Cats）、组织蛋白酶K（CatK）在曲张大隐静脉平滑肌细胞（SMC）中的表达变化。方法：术中收集曲张及正常大隐静脉标本，采用免疫组织化学显色方法、免疫印迹法检测CatS、CatK在曲张和正常大隐静脉管壁的表达。结果：CatS和CatK在曲张大隐静脉中免疫反应阳性，阳性细胞主要位于SMC胞质，曲张组SMC中CatS和CatK阳性细胞平均光密度值较正常组明显增高，其蛋白表达明显高于正常组，免疫荧光显色可见CatS、CatK与曲张大隐静脉SMC共定位。结论：CatS和CatK在曲张大隐静脉SMC中表达明显增高，表明两者可能参与了静脉曲张的发生，并可能作为该疾病的生物学标志。     

人脑桥静脉平滑肌细胞α-肌动蛋白的表达

方法:人脑标本12例,共计62支桥静脉.应用H-E染色、丽春红-维多利亚蓝组合染色观察桥静脉SMC的组织学特点,运用免疫组织化学显色、免疫荧光化学显色和免疫印迹法检测平滑肌特异标记物α-平滑肌肌动蛋白(α-SMA)的表达.结果:桥静脉管壁中膜可见散在、孤立的SMC,大多呈纵切面,α-SMA在桥静脉中免疫反应阳性;桥静脉流出端硬脑膜侧α-SMA表达高于蛛网膜侧.结论:桥静脉管壁中有SMC的存在,且分布不对称.     

巢蛋白及性别决定基因高迁移率组蛋白在SAMP8小鼠穹窿下器的表达及其意义

目的 观察巢蛋白（Nestin）与性别决定基因高迁移率组蛋白(SOX2)在快速老化小鼠SAMP8穹隆下器（SFO）中的表达。 方法 成年8月龄SAMP8小鼠设为实验组,采用免疫组织化学和免疫组织化学荧光染色的方法观察Nestin及SOX2在穹隆下器中的表达,同时设正常老化小鼠SAMR1对照。结果 Nestin免疫组织化学染色发现,对照组免疫阳性细胞呈突起分布,放射丝状表达;实验组免疫阳性细胞染色深,丝状结构粗大,在血管周围密集分布,阳性细胞百分比（49.17±7.60）%较对照组阳性细胞百分比（16.33±4.41）%明显增多,差异有统计学意义（P＜0.01）。SOX2免疫组织化学染色,对照组免疫阳性细胞散在分布,染色深浅不一;实验组大部分阳性细胞染色较深,在血管周围密集分布;阳性表达细胞百分比（62.17±20.27）%较对照组阳性表达细胞百分比（36.00±16.20）%明显增多,差异有统计学意义（P＜0.05）。荧光双标染色,对照组SOX2散在分布,其间可见Nestin阳性表达,室管膜下区可见少量SOX2/Nestin双表达细胞。实验组阳性表达强烈,SOX2/Nestin双表达细胞增多。结论 Nestin及SOX2在快速老化小鼠SAMP8穹隆下器的表达明显增强,表明阿尔茨海默病（AD）在一定时期可能引起穹隆下器神经干细胞/祖细胞的增殖或分化增强,进而可能影响穹隆下器的神经生发功能。     

巢蛋白及性别决定基因高迁移率组蛋白在SAMP8小鼠穹隆下器的表达及意义

目的观察巢蛋白(Nestin)与性别决定基因高迁移率组蛋白(SOX2)在快速老化小鼠SAMP8穹隆下器(SFO)中的表达。方法成年8月龄SAMP8小鼠设为实验组,采用免疫组织化学和免疫组织化学荧光染色的方法观察Nestin及SOX2在穹隆下器中的表达,同时设正常老化小鼠SAMR1对照。结果 Nestin免疫组织化学染色发现,对照组免疫阳性细胞呈突起分布,放射丝状表达;实验组免疫阳性细胞染色深,丝状结构粗大,在血管周围密集分布,阳性细胞百分比(49.17±7.60)%较对照组阳性细胞百分比(16.33±4.41)%明显增多,差异有统计学意义(P0.01)。SOX2免疫组织化学染色,对照组免疫阳性细胞散在分布,染色深浅不一;实验组大部分阳性细胞染色较深,在血管周围密集分布;阳性表达细胞百分比(62.17±20.27)%较对照组阳性表达细胞百分比(36.00±16.20)%明显增多,差异有统计学意义(P0.05)。荧光双标染色,对照组SOX2散在分布,其间可见Nestin阳性表达,室管膜下区可见少量SOX2/Nestin双表达细胞。实验组阳性表达强烈,SOX2/Nestin双表达细胞增多。结论 Nestin及SOX2在快速老化小鼠SAMP8穹隆下器的表达明显增强,表明阿尔茨海默病(AD)在一定时期可能引起穹隆下器神经干细胞/祖细胞的增殖或分化增强,进而可能影响穹隆下器的神经生发功能。     

硒对氧化偶氮甲烷所致大鼠结肠癌形成过程中垂体远侧部促甲状腺激素阳性细胞的影响

目的:观察硒对氧化偶氮甲烷(AOM)所致大鼠结肠癌形成过程中,垂体远侧部促甲状腺激素(TSH)阳性细胞的影响.方法:SD 大鼠随机分为正常对照、实验对照、致癌剂前补硒和致癌剂后补硒组.34周取垂体,用免疫组织化学 SP 法和SAP法,观察其远侧部 TSH 阳性细胞的形态结构及免疫组织化学反应强度,并进行图像分析.结果:亚甲蓝染色光镜下观察,可见 AOM 腹腔注射的大鼠结肠黏膜出现异常隐窝和异常隐窝灶.免疫组织化学显示,与正常对照组相比,实验对照组 TSH 阳性细胞反应显著减弱;硒干预各组与实验对照组相比,TSH 阳性细胞的阳性反应进一步减弱.结论:硒参与了AOM 所致大鼠结肠癌形成过程中,垂体远侧部 TSH 阳性细胞功能变化的调节.     

人静脉移植物中MCP-1的表达

目的：探讨人静脉移植物中单核细胞趋化蛋白-1（MCP-1）的表达及其与巨噬细胞浸润的关系。方法：应用免疫荧光组织化学技术对30个静脉移植物再塞标本中MCP-1、CD68（巨噬细胞的标记物）、CD3l（内皮细胞的标记物）的表达进行了检测，用激光共聚焦显微镜拍片，图片用Silicon Graphics Octane进行处理。结果：在正常静脉血管中，MCP-1表达很弱；外膜中有少量CD68免疫阳性细胞，中膜和内膜中少见；CD31免疫阳性细胞仅在血管腔内皮细胞和外膜小血管中可见。在病变静脉血管，MCP-1在内皮细胞、平滑肌细胞中的表达呈强阳性；CD68免疫阳性细胞在外膜、中膜和内膜均可见到；CD31免疫阳性细胞不仅出现在血管腔内皮细胞和外膜小血管内皮细胞，且在中膜小血管内皮细胞也大量出现。免疫双重染色显示内皮细胞和巨噬细胞均表达MCP-1。结论：人静脉移植物MCP-1的表达上调，且和巨噬细胞的浸润关系密切，提示MCP-l对静物移植物炎症细胞的浸润及新内膜的形成有非常重要的作用。     

中药髓复康对恒河猴脊髓半横断后凋亡相关蛋白表达的影响

目的:探讨中药髓复康(SFK)对恒河猴脊髓半横断后凋亡相关蛋白(Bcl-2、Bax和p53)表达的作用及其机理。方法:实验选用8岁恒河猴14只,随机分为中药治疗组6只、横段脊髓模型组6只、正常对照组2只,采用双盲法复制脊髓T12右侧半横断损伤模型。灌喂SFK溶液1月后,实验恒河猴麻醉、以损伤部位为中心切取长1cm的脊髓,采用常规SABC免疫组织化学方法检测凋亡相关基因Bcl-2、Bax和p53蛋白表达,并对结果进行定性、定位、图像分析和统计学处理。结果:脊髓Bax免疫组织化学阳性细胞的平均光密度(MOD)模型组略高于正常对照组,中药组明显低于模型组(P0.05)。脊髓Bcl-2免疫组织化学阳性细胞面数密度(NA),中药组明显高于模型组(P0.05)。脊髓Bcl-2免疫组织化学阳性细胞的MOD模型组略高于正常对照组,中药组略高于模型组。脊髓p53免疫组织化学阳性细胞NA,模型组明显高于正常对照组(P0.05),中药组明显低于模型组(P0.05)。脊髓p53免疫组织化学阳性细胞的MOD模型组明显高于正常对照组(P0.05),中药组明显低于模型组(P0.05)。结论:髓复康可以促进Bcl-2蛋白表达,抑制Bax、p53蛋白表达和抑制神经元凋亡,对脊髓神经损伤具有保护作用。     

Promoting effect of atorvastatin on vascularization for type Ⅰ collagen scaffold in rat

目的:探讨阿托伐他汀能否作为促血管新生药物促进组织工程移植物的血管化.方法:通过伊红染色、扫描电镜观察Ⅰ型胶原蛋白支架的形态及表面结构,然后将支架植入大鼠体内,阿托伐他汀灌胃.做免疫组织化学显色和RT-PCR,检测血管内皮生长因子(VEGF)和CD34蛋白和基因的表达情况.结果:伊红染色可见,支架结构疏松、多孔.扫描电镜可见支架表面光滑,有少许皱褶.免疫组织化学结果可见CD34阳性细胞多位于支架边缘,均匀分散于胞膜中,VEGF染色阳性颗粒分布于细胞质或细胞外基质中.实验组阳性细胞表达较对照组多.RT-PCR结果显示实验组VEGF的基因表达较对照组高,差异具有统计学意义.结论:阿托伐他汀可以促进大鼠体内Ⅰ型胶原蛋白支架中VEGF基因的表达,刺激VEGF的分泌,增加CD34阳性细胞数量.     

中药髓复康对恒河猴脊髓半切后S-100、tau蛋白表达的影响

目的:探讨中药髓复康对恒河猴脊髓半切后S-100、 tau蛋白表达的作用及其机制.方法:8岁恒河猴,随机分为中药治疗组、横段脊髓模型组和正常对照组.采用双盲法复制脊髓胸12右侧半横断损伤模型,灌胃髓复康1个月后,实验恒河猴以损伤部位为中心切取长1cm的脊髓,采用常规SABC免疫组织化学方法检测S-100、 tau蛋白表达.结果:脊髓S100蛋白免疫组织化学阳性细胞数量,模型组略高于正常对照组,中药组略高于模型组.脊髓S100蛋白免疫组织化学阳性细胞的平均光密度(MOD),模型组略低于正常对照组,中药组略高于模型组.脊髓tau蛋白免疫组织化学阳性细胞数量,模型组和中药组略低于正常对照组.脊髓tau蛋白免疫组织化学阳性细胞的平均光密度,模型组略高于正常对照组,中药组略高于模型组,明显高于正常对照组.结论:髓复康可以改善再生环境,促进微管相关蛋白tau的表达,促进轴突再生.     

自制弧形槽针在治疗单纯性大隐静脉曲张中的应用（附30例报告）

目的探讨采用弧形槽针做皮内缝合结扎曲张静脉的手术方式治疗单纯性大隐静脉曲张的治疗效果。方法选择30例单纯性大隐静脉曲张患者35条下肢,通过使用自制的弧形槽针在皮内环绕曲张静脉缝合结扎,将线结置于皮下;在常规大隐静脉结扎切口下方做切口,显露出大隐静脉主干,在分支下方用粗丝线双重结扎,不切断抽除大隐静脉主干与传统手术方式进行比较。结果随访6个月至2年半静脉曲张无一例复发,皮内缝合处针孔疤痕全部消失。结论采用弧形槽针治疗大隐静脉曲张的治疗方法安全可靠、简便省时、费用低、创伤小、小腿无疤痕,值得临床推广。     

Collagen alteration in vascular remodeling by hemodynamic factors

The collagen alterations in the vascular wall remodeled by hemodynamic change were investigated by electron microscopy and immunohistochemistry. The left anterior descending coronary artery (LAD) without a myocardial bridge (MB) showed both lower matrix metalloproteinase-1 (MMP-1) expression and a smaller extent of spiraled collagen (SC) distribution than the LAD wall with MB, in which the intima was influenced by high shear stress. In the wall of the varicose great saphenous vein (GSV) the expression of MMP-1 was lower, while the expression of prolyl 4-hydroxylase was higher, than in the normal GSV. The extent of SC distribution in the intima and media of the varicose GSV was smaller than that in the normal GSV. An analogous difference in results was demonstrated between the portal vein (PV) of patients with liver cirrhosis and normal PV. However, the levels of expression of MMP-2, MMP-9 and tissue inhibitors of MMP (TIMPs) in these pathologic vessels were not different from those in the corresponding normal vessels. The results indicate that hemodynamic forces such as shear stress and increased intravascular blood pressure contribute to the collagen alterations in the vascular wall, which may lead to vascular wall remodeling. Received: 22 October 1999 / Accepted: 20 January 2000     

Endothelial mediated enhancement of noradrenaline induced vasoconstriction in normal and varicose veins

The role of the endothelium to influence smooth muscle contraction in normal and varicose veins was investigated in vitro using saphenous vein preparations obtained at vascular surgery. Paired control and endothelium-denuded rings were tested simultaneously and contracted with noradrenaline (10(-9)-10(-4) M). Identical experiments were also performed on sheep femoral veins and arteries. Noradrenaline induced maximal tension was 30% lower in varicose compared to normal veins. In normal veins removal of endothelium significantly reduced the maximal response to noradrenaline by 40% whereas in varicose veins no significant reduction could be seen. In the sheep femoral vein removal of the endothelium also resulted in decrease of noradrenaline-induced contraction. It can be concluded that in the human saphenous vein the endothelium has a contraction facilitating effect in response to stimulation with noradrenaline. In varicose veins the endothelial-mediated enhancement of noradrenaline-induced vasoconstriction is reduced probably because of endothelial damage. This observation may be of importance in the pathogenesis of varicose veins.     

Cultured human atherosclerotic plaque smooth muscle cells retain transforming potential and display enhanced expression of the myc protooncogene

The proliferation of vascular smooth muscle cells (SMC) is critical to atherosclerotic plaque formation. The monoclonal hypothesis proposes that the stimulus for this SMC proliferation is a mutational event. Here we describe a procedure for growing human plaque smooth muscle cells (p-SMC) in culture. We show that p-SMCs derived from two patients differ from SMC cultured from normal vascular tissue in expression of the protooncogene myc. One p-SMC strain was extensively characterized; these diploid, karyotypically normal cells have a finite life span in culture. Ultrastructural examination revealed two populations, one with classic contractile SMC appearance, the other, modulated to a synthetic state. Northern blotting showed a 2- to 6-fold and a 6- to 11-fold enhanced expression of myc by p-SMC, compared to SMC derived from healthy human aorta (HA-SMC) and saphenous vein (HV-SMC), respectively. In contrast, the p-SMC and HV-SMC expressed similar levels of message for the genes N-myc, L-myc, Ha-ras, fos, sis, myb, LDL receptor, EGF receptor, IGF I receptor, IGF II, and HMG CoA reductase. Finally, although p-SMCs are not tumorigenic, DNA isolated from these cells is positive in the transfection-nude mouse tumor assay. Myc, however, does not appear to be the transforming gene because no newly introduced human myc gene was detected in the p-SMC-associated nude mouse tumor. Thus human atherosclerotic p-SMCs possess both an activated myc gene and a transforming gene that is retained throughout many cell passages.     

Anatomical observation on draining patterns of saphenous tributaries in Korean adults.

This study was done to identify the normal and variants of saphenous tributaries in Korean adults. The pattern of confluence of saphenous tributaries, medial accessory saphenous, lateral accessory saphenous, superficial epigastric, superficial circumflex iliac and superficial external pudendal veins, was carefully examined in 249 lower limbs (right, 129; left, 120) of embalmed Korean cadavers (73 males & 56 females). The medial accessory saphenous vein drained into the great saphenous vein directly (in 82.3%) or by a common trunk (in 17.7%) with the superficial epigastric or superficial external pudendal vein. The lateral accessory saphenous vein entered the great saphenous (in 67.1%) or the femoral vein (in 32.9%) directly or, forming a common trunk with other saphenous tributaries. The superficial epigastric vein joined the great saphenous (in 77.1%) or the femoral vein (in 22.9%) directly or, by a common trunk with other saphenous tributaries. The superficial circumflex iliac vein reached the great saphenous (in 83.1%) or the femoral vein (in 16.9%) directly or, by a common trunk with other saphenous tributaries. The superficial external pudendal vein opened into the great saphenous (in 95.2%) or the femoral vein (in 4.8%) directly or by a common trunk with other saphenous tributaries. In Koreans, the incidence of the normal pattern of saphenous tributaries was 23.7% and in 76.3% any one of variant saphenous tributaries entered the femoral or the great saphenous vein by a common trunk with other saphenous tributaries.     

Plasma concentration of thymus and activation-regulated chemokine is elevated in childhood asthma

BACKGROUND: The role of IL-5-induced eosinophilia in airway hyperresponsiveness has been questioned. In addition, eosinophil-independent IL-5-induced airway hyperresponsiveness has been demonstrated in animals. OBJECTIVE: In this study, IL-5 was investigated for direct effects on human bronchial responsiveness. METHODS: Human muscle preparations were isolated from organ donor and surgical tissue. Bronchus, jejunum, and saphenous vein were incubated for 24 hours in vitro with recombinant human (rh) IL-5. Contractility to acetylcholine (bronchus, jejunum) and phenylephrine (saphenous vein) was then investigated. RT-PCR was used to evaluate IL-5 receptor alpha (IL-5R(alpha)) expression in various tissues and to assess bronchus and saphenous vein eosinophils through use of CCR3 expression. RESULTS: rhIL-5 primed bronchus for an exaggerated contraction to acetylcholine. The acetylcholine concentration that produced 50% of the control maximum response was reduced 17- to 20-fold in bronchus treated with 1 and 10 nmol/L rhIL-5. The lower concentration of 0.1 nmol/L rhIL-5 had no effect. The rhIL-5 effect on bronchial contractility was attenuated by antibodies to IL-5 (TRFK-5; 100 nmol/L) and human IL-5R(alpha) (100 nmol/L). rhIL-5 (10 nmol/L) did not enhance contractility of saphenous vein or jejunum. When RT-PCR was used, IL-5R(alpha) expression was strong in bronchus muscle, weak in trachealis, saphenous vein, and atrial muscle, and undetectable in jejunum, urinary bladder, and pulmonary and renal artery muscle. Comparable weak expression of CCR3 was identified in bronchus and saphenous vein. CONCLUSION: The findings are consistent with an airway tissue-selective expression of the IL-5 receptor that mediates IL-5-induced airway hyperresponsiveness independent of eosinophils. In asthma, in which IL-5 expression is elevated, IL-5 might directly induce bronchial hyperresponsiveness.     

彩超对曲张静脉管径和交通静脉反流与临床分级的相关性研究

目的应用彩色多普勒超声（彩超）对静脉曲张的患肢及正常肢体进行检查，探讨小腿交通静脉反流对临床分级的影响，并对比观察观察组及正常组下肢静脉的管径。方法站立位时通过彩色多普勒超声对观察组25例患者的39条患肢与正常组10例患者（健康下肢）和正常人的12条健康下肢进行检查，对比观察有无小腿交通静脉反流、静脉管径等参数；对小腿交通静脉反流与临床分级作相关分析。结果正常组小腿交通静脉管径均小于3mm．未见交通静脉反流。观察组小腿交通静脉管径大于4mm伴有反流者18条下肢，小腿交通静脉反流与临床分级具有相关性（Fisher＇s exact=0．005），有小腿交通静脉反流者临床分级更高。站立位观察组患者的下肢大隐静脉、股总静脉、股浅静脉、胭静脉管径较正常组健康者的增宽。结论有小腿交通静脉反流者临床分级更高，故应常规检查小腿交通静脉；下肢静脉曲张组静脉管径较正常者的增宽，因此，发现静脉管径明显增粗时，应观察静脉有无反流。     

Localization of cysteine protease, cathepsin S, to the surface of vascular smooth muscle cells by association with integrin alphanubeta3

Smooth muscle cell (SMC) migration from the tunica media to the intima, a key event in neointimal formation, requires proteolytic degradation of elastin-rich extracellular matrix barriers. Although cathepsin S (Cat S) is overexpressed in atherosclerotic and neointimal lesions, its exact role in SMC behavior remains primarily unresolved. We examined the involvement of Cat S on SMC migration through an extracellular matrix barrier and its localization in SMCs. A selective Cat S inhibitor and the endogenous inhibitor cystatin C significantly attenuated SMC invasion across elastin gel. Western blotting and cell surface biotinylation analysis demonstrated localization of the 28-kd active form of Cat S on the SMC surface, consistent with its role in the proteolysis of subcellular matrices. Treatment with interferon-gamma or interleukin-beta1 significantly augmented the ability of SMC membranes to degrade elastin along with a significant increase in the level of active Cat S compared with controls. Immunofluorescence and confocal microscopy showed a punctuated pattern of Cat S clusters at the periphery of SMCs; further studies demonstrated partial co-localization of Cat S and integrin alphanubeta3 at the cell surfaces. These findings demonstrate that active Cat S co-localizes with integrin alphanubeta3 as a receptor on the SMC surface, playing an important role in the invasive behavior of SMCs.     

大隐静脉射频消融术与大隐静脉高位结扎剥脱术治疗静脉曲张的疗效对比

目的比较大隐静脉射频消融术和大隐静脉高位结扎剥脱术治疗静脉曲张的临床疗效。方法回顾性分析2018年9月至2019年5月江苏省中医院收治的70例(93条患肢)下肢大隐静脉曲张患者的临床资料,将行大隐静脉射频消融术和高位结扎剥脱术治疗的患者分别设为观察组和对照组,每组35例(对照组43条患肢,观察组50条患肢)。对比分析2组患者手术时间、恢复时间、切口数量、术后VAS疼痛评分等手术相关情况,比较2组患者手术前后CEAP分级、临床疗效、术后并发症、术后生活质量和满意度评分。结果与对照组相比,观察组所需手术时间、术后首次下床时间、住院时间及恢复时间较短,术中出血量、切口数量较少,术后VAS疼痛评分较低;2组患者CEAP分级构成在术后均比术前显著降低;观察组患者术后并发症总发生率显著低于对照组;观察组患者术后生活质量总评分及各项评分均显著高于对照组;观察组患者对治疗方法的整体评价高于对照组,以上差异均具有统计学意义(P<0.05)。结论大隐静脉射频消融术与大隐静脉高位结扎剥脱术疗效相当,但射频消融术有操作简单、创伤小、恢复快、并发症少且美观等优势,同时可减轻患者痛苦,显著改善术后生活质量,提高患者满意度。     

血胱抑素C检测对围产期新生儿高胆红素血症肾脏损害的评估价值

目的探讨围产期新生儿高胆红素血症血胆红素、血胱抑素C的变化及其对肾脏损害的评估价值。方法选择2009年10月-2010年10月在本院住院的新生儿高胆红素血症患儿44例为观察组,按黄疸程度分为轻度黄疸,220-265μmol/L,中度黄疸265-342μmol/L,重度黄疸≥342μmol/L,对照组为同期在本科住院的无新生儿高胆红素血症的新生儿40例,所有患儿在入院当天抽股静脉血3毫升,查肝功、肌酐、尿素氮、β2微球蛋白及胱抑素C,黄疸消退后复查血胆红素及胱抑素C,并做统计学处理。结果轻度组21例,中重度组23例,对照组40例,观察组与对照组在总胆红素及间接胆红素数值有显著性差异(P<0.01);观察组与对照组肌酐、尿素氮无显著性差异(P>0.05);观察组胱抑素C、β2微球蛋白显著高于对照组(P<0.01,P<0.05),提示黄疸程度越重,血胱抑素C及β2微球蛋白值越高,肾脏损害越重,经治疗后,观察组总胆红素、胱抑素C及β2微球蛋白水平明显下降,与治疗前比较有显著性差异(P<0.01,P<0.05),观察组治疗后血胱抑素C显著下降,与对照组比较无显著性差异(P>0.05),观察组血胱抑素C、β2微球蛋白、尿素氮与总胆红素值之间的关系:血清CysC、β2-MG与TBil值呈正相关(r分别为0.71、0.42,P均<0.01);尿素氮、肌酐与总胆红素值之间无相关性(r=0.24,P>0.05)。结论新生儿高胆红素血症可造成肾脏损害,围产儿尤其明显,但它对肾脏损害是暂时的,可逆的,通过对新生儿高胆红素血症的患儿检测血胱抑素C,可以早期发现肾损害,避免使用肾损伤药物,及早进行干预,提高本病的治愈率。