The effect of pre-incubation and direct procedure for the alanine transaminase assay in two kits compared.

The kinetic measurement of serum alanine transaminase was carried out on two enzyme kits (Roche and Calbiochem), done as a direct method and a pre-incubation method. Variable results from commercial control sera and patient sera by these four procedures were obtained. Pre-incubation enzyme values were generally lower than the direct values (mean difference 10%), but there were marked differences between the results from the two kits. This finding has important significance especially for laboratories participating in external QC Programmes. Frequent precision checks of the method and a local reference range are therefore necessary.     

患者中心疗法对2型糖尿病患者心理行为的影响

目的 探讨患者中心疗法对糖尿病患者心理状况及行为的影响.方法 将60例糖尿病患者随机分为对照组(30例)和干预组(30例),对干预组进行患者中心疗法干预,干预前后用症状自评量表(SCL-90)和糖尿病患者行为评估表对两组患者心理状况和行为状态进行评定.结果 干预前两组患者在SCL-90量表各因子上得分的差异无统计学意义(P>0.05),干预后两组比较,躯体化、强迫、人际关系敏感、敌对、恐怖、偏执、精神病性等因子均统计学意义(P<0.01);干预后两组患者在不良饮食习惯纠正、戒酒、复查时间的掌握及在血糖控制、坚持运动锻炼、戒烟方面的差异有统计学意义(P<0.01),按时服用降精药物或注射胰岛素无统计学意义(P>0.05).结论 患者中心疗法对提高糖尿病患者的心理健康水平,增加其健康行为有积极的作用.     

Evaluation of serum digoxin and serum cortisol by radioimmunoassay with the Concept-4.

The solid-phase radioimmunoassay procedures for serum digoxin and cortisol with the Concept-4 were evaluated for specificity, correlation with other radioimmunoassay procedures, parallelism, recovery, carryover, and precision. 1. The detection limits of the digoxin and cortisol assays were 10 pg/tube and 40 pg/tube, respectively. These detections correspond to serum concentrations of 0.25 micrograms/liter and 20 micrograms/liter, respectively, for digoxin and cortisol. 2. Specificity of the digoxin assay was characterized by a 18.3% cross reaction with digitoxin. The anti-cortisol with 11-deoxycortisol, cross reaction with 11-deoxycortisol. 3. The values obtained with the Concept-4 for both procedures demonstrated acceptable correlations with other existing RIA procedures. 4. Dilution studies conducted to determine the parallelism of the 2 assays indicated that both assays demonstrated excellent proportionality between the volume of specimen and the quantity of analyte measured. 5. Analytical recoveries of digoxin and cortisol added to the individual assays average 95% and 101%, respectively. 6. Carryover studies indicated a routine sample-to-sample interaction of less than 0.05% for both assays. 7. The digoxin assay routinely demonstrated intra- and inter-assay variations of less than 7.4% and less than 10.0%, respectively. The precision data for cortisol indicated intra- and inter-assay variations of less than 6.7% and less than 9.1%, respectively.     

Defective erythrocyte pyruvate kinase.

A defective pyruvate kinase (EC 2.7.1.40) is described. The abnormal PK is characterized by a shift in the R in equilibrium T equilibrium to the T-state. The Ko.5 for the substrate phosphoenol pyruvate is about 6 times higher than for the normal enzyme, while the KM value for the positive effector Fru-1, 6-P2 is increased. In agreement with a shift to the T-state is the increased affinity of the abnormal enzyme for the negative effectors ATP and alanine. The results are discussed in relation to other abnormal pyruvate kinases.