芸香甙-PVP共沉淀物在食用油脂中的抗氧化研究

将天然黄酮类化合物芸香甙与聚乙烯吡咯烷酮(PVP)制备成共沉淀物后,研究其在食用油脂中的抗氧化作用。实验结果表明芸香甙-PVP在油脂中的浓度>0.010%时,有较强的抗氧化作用。将芸香甙-PVP与BHA、PG在油脂中对比抗氧化作用,发现当浓度相同时,其抗氧化作用相似。     

丹参生药颗粒饮片及饮片提取颗粒中原儿茶醛含量的比较

目的：研究丹参生药颗粒代替饮片。方法：采用正交试验法优选凡参生药颗粒制备工艺条件,并用紫外分光光度法经颗粒、饮片汤剂及免煎饮片中的主要水溶性成分原儿茶醛含量,对浸出率进行比较。结果：丹参生药照颗粒的优选工艺条件是：药材粒度为５目,加水量是药材的２０倍,煎煮３次,每次１５ｍｉｎ。３种人煎汤剂中,生药颗粒的煎出率最高,是饮片的１．１５倍,免煎剂的３．１倍。原儿茶醛含量也以袋包颗粒是最高,是饮片的１．２     

益欣康泰胶囊质量标准的研究

目的：为了控制益欣康泰胶囊质量提供科学依据;方法;对益欣康泰胶囊主要成分进行了薄层鉴别,用薄层扫描法测定制剂中大黄素的含量并测定其浸出;结果：（１）益欣康泰胶囊中甘草及甘草酸铵得到的较好的分离、与对照品,对照药材对应;（２）大黄素含量为０．５６－０．９１ｍｇ／粒,（３）正丁酸浸出物含量为０．９－３．２ｍｇ／粒,结论：方法简便,快速,准确。     

微波联合湿润烧伤膏治疗尿道尖锐湿疣

目的：探讨微波联合湿润烧伤膏（MEBO）治疗尿道尖锐湿疣的疗效。方法：应用微波对60例尿道尖锐湿疣患进行治疗，随后用MEBO涂患处30例，并与微波联合红霉素软膏治疗（30例）进行比较。结果：微波联合MEBO外用疗效优于微波联合红霉素软膏（P＜0．01）。结论：微波联合MEBO对尖锐湿疣有良好协同治疗作用。     

中医自拟中药槐花祛紫汤治疗小儿过敏性紫癜疗效观察

目的:探讨应用自拟中药槐花祛紫汤治疗小儿过敏性紫癜的临床疗效.方法:将76例患儿随机分成两组,治疗组根据中医临床分型、辨证加减使用自拟槐花祛紫汤治疗,对照组采用常规西药治疗.2周后观察两组患者的疗效.结果:治疗组40例中,治愈37例,有效2倒,无效1例;对照组36例中,治愈30例,有效5例,无效1例.两组综合疗效比较,治疗组治愈率明显优于对照组.结论:自拟中药槐花祛紫汤治疗小儿过敏性紫癜有较好的疗效.     

何首乌中二苯乙烯甙的含量测定

目的 :考察药材市场的生、制首乌的药材质量。方法 :采用ZorbaxSB C18柱 ,用乙腈 水 (2 5∶75 )为流动相 ,在32 0nm处检测二苯乙烯甙的含量。结果 :二苯乙烯甙平均含量生首乌为 1 4 0 16 % ,制首乌为 2 336 8%。结论 :清平药材市场的生、制首乌药材质量基本合格     

HPLC高效液相色谱仪的维修

液相色谱是以液体作为流动相的色谱分析方法。高效液相色谱仪在医药、生化方面的应用主要有：分析体液内异常代谢物质，分析药物的组分含量，药物生产中进行中间控制，分析药物在体内的残留量，测定药物在各器官中的代谢产物，定性测定细胞核中的甙及甙酸，分析核酸以及分析氨基酸、酶、糖等。     

高效毛细管电泳法测定何首乌中二苯乙烯甙的含量

目的：建立测定何首乌中二苯乙烯甙含量的高效毛细管电泳法。方法：电解液为２０ｍｍｏｌ·Ｌ＾－１硼砂,检测波长２１４ｎｍ,二苯乙烯甙为对照品,测定了何首乌及其制剂中二苯乙烯甙的含量。结果：进样量在１０．０￣１００．０μｇ·ｍｌ＾－１范围内线性关系良好（ｒ＝０．０９９９９）,加样回收率为９８．７１％,ＲＳＤ为１．５１％（ｎ＝３）,测得生何首乌,制何首乌及优生宝胶囊中二苯乙烯甙的含量分别为０．１２７％、０     

正交实验法优化贞芪增免胶囊有效成分的提取工艺

目的以药材指标成分和出膏量为指标优选贞芪增免胶囊有效成分的提取工艺。方法以黄芪甲苷、阿魏酸为指标成分,采用正交实验法对贞芪增免胶囊有效成分的提取工艺进行优选。结果优选工艺为：黄芪等3味药材,采用回流法（8倍量70％乙醇,提取3次,每次1h）;当归等其它药材及醇提取后的药渣采用水煎煮法（10倍量水,提取3次,每次1h）。结论该工艺合理,有效成分提取率高。     

高效液相色谱仪的维修

液相色谱是以液体作为流动相的色谱分析方法，其在医药、生化方面的应用主要有：分析体液内异常代谢物质，分析药物的组分含量，药物生产中进行中间控制，分析药物在体内的残留量，测定药物在各器官中的代谢产物，定性测定细胞核中的甙及甙酸，分析核酸以及分析氢基酸、酶、糖等。     

芦丁壳聚糖缓释片处方设计及体外释放度测定

目的 研究以壳聚糖为骨架制备芦丁壳聚糖缓释片的工艺.方法 以壳聚糖、阿拉伯胶和淀粉为辅料制备芦丁壳聚糖缓释片,以正交设计法进行处方筛选,用释放度实验考察芦丁壳聚糖缓释片不同释放时间的溶出参数.结果 含壳聚糖1.5%、阿拉伯胶1.5%和淀粉0.5%时所制备的芦丁片具有明显的缓释作用,其T50为(123.97±0.47)m...     

超微粉碎技术的残黄片溶出度研究

目的研究超微粉碎技术对残黄片溶出度的影响。方法采用超微粉碎技术对原料药材进行粉碎,压片,以盐酸溶液（pH 1.0）为溶出介质,采用桨法测定体外溶出度,比较基于超微粉碎技术的残黄片和普通粉碎的残黄片的溶出速率和溶出量。结果基于超微粉碎技术的残黄片的体外累积溶出率相比基于普通粉碎的残黄片有显著提高。结论超微粉碎技术工艺简单易行,可提高残黄片有效成分的溶出。     

延胡索醇回流法与超声法提取工艺比较研究

目的探讨延胡索药粉超声波提取的可行性,为工业化生产提供依据。方法以延胡索乙素为评价指标,首 先采用正交设计试验法筛选延胡索药粉超声波提取与醇回流提取各自的最优工艺条件,再用配对t检验法分析实验数据。结 果延胡索醇超声提取最优工艺参数为：延胡索药粉加人50%乙醇浸泡30 min,50%乙醇体积为药材量的6倍,超声时间1.0h。延胡索醇回流提取最优工艺参数为：延胡索药粉加入50%乙醇浸泡30 min,50%乙醇体积为药材量的10倍,水浴回流时间1.0 h。延胡索药粉醇回流与超声提取差别有统计意义,回流提取优于超声提取。结论延胡索采用回流提取为佳,按最 优工艺条件提取,延胡索乙素含量最高。     

制片工艺对芦丁缓释骨架片释药机制影响研究

目的：考察制片工艺对芦丁缓释骨架片释药机制的影响情况。方法：以羟丙基甲基纤维素(HPMC)为骨架材料制备缓释骨架片，利用Peppas经验式释放指数n值，评价制片工艺对芦丁缓释骨架片体外释药机制的影响。结果：干法制片的释药比湿法快。结论：干法制片与湿法制片有相同的释药机制。     

Determination of the diffusion function of 25 MV x-rays in a tissue equivalent medium]

The diffusion function of 25 MV Roentgen rays in a tissue-equivalent material was determined experimentally as a function of the size of the beam and of the depth into the material. In order to characterize the beam, the attenuation of the beam in various materials was previously investigated. The results of the investigation demonstrate the important contribution of diffused radiation to the dose when using very high energy radiation.     

A DNA extraction method of small quantities of bone for high-quality genotyping

DNA genotyping techniques have been used successfully in forensic science for almost three decades and represent the gold standard for individual identification. However, efficient protocols for obtaining DNA from exhumed bones suitable for genotyping are still scarce and most of them require a considerable amount of starting material, are time consuming and are inefficient for reducing inhibitor's effects. We sought to develop an optimised protocol for extracting DNA from bone samples obtained from exhumations. We tested two approaches for preparing bone samples: (a) fine powder and (b) thin slices of bone. The best ratio of bone amount to DNA yields was assessed by a titration experiment using bone powder ranging from 50 to 1000 mg. We obtained optimal DNA yields (27 pg mg⁻¹ on average) when 150–200 mg of starting material were processed using a one-step demineralisation method. Better-quality profiles (determined by the number of genotyped loci) were obtained when DNA was extracted from bone slices compared to extraction from bone powder. From bone slices 83.9% and from bone powder 46.7% of the samples provided genotypes for 11 or more loci. Since bone preparation procedures were carried out at room temperature, the method developed in the present study might be an attractive alternative to the standard freeze-mill approach, being faster and more cost-efficient.     

Evaluation of the 123I-IMP Patlak plot method using the pulmonary differential curve as an input function: a comparison with cerebral blood flow (CBF) determined by the noninvasive micro-sphere (NIMS) method

We explored the possibility of applying the Patlak plot method to clinical practice as a simple non-invasive quantitative method of measuring cerebral blood flow using N-isopropyl-4-[123I]iodoamphetamine (123I-IMP). On the assumption that after temporarily accumulating in the lungs, all the administered 123I-IMP is eliminated by the pulmonary arterial flow for systemic diffusion, we collected dynamic data by setting an area ranging from the brain to the whole lung field within the field of the camera. The lung clearance curve L(t) was differentiated and divided by cardiac output. It was then converted a positive number by multiplying it of--1 to determine the volume of 123I-IMP tracer diffused in arterial blood per unit of time. The calculated concentration was defined as the arterial time activity curve A(t). A Patlak plot analysis was conducted between A(t) and the brain time activity curve B(t) to determine K1 (total cerebral blood flow [tCBF], ml/min) and Vn (nonspecific initial distribution volume, ml). The total volume of tracer diffused in the central cardiovascular system within a given (T) was also obtainer from the volume of tracer remaining in the lungs [Lpeak--L(T)], and by reporting this calculation over time, an accumulation curve was produced. By differentiating the obtained accumulation curve, we were able to estimate the volume of tracer diffused in the central cardiovascular system per unit of time. With this value used as the input function index I(t), a Patlak plot analysis was conducted to determine the unidirection influx index ki, which was then multiplied by 100 to obtain the brain perfusion index (IMP-BPI). The noninvasive micro-sphere method was performed concurrently on 16 patients with cerebrovascular and/or neurological disorders to obtain mean cerebral blood flow (mCBF). Correlations between K1 and between IMP-BPI and mCBF were then determined and compared. Both K1 and IMP-BPI obtained from ki were found to correlate highly with mCBF, r = 0.759 (y = 0.032x + 20.1) and r = 0.833 (y = 2.73x + 0.10) respectively, with a better result from IMP-BPI. These results indicate that the 123I-IMP Patlak plot method with a wide-field gamma camera is clinically applicable as a simpler noninvasive technique for measuring cerebral blood flow even when a simple input function is used.     

DWI及ADC值在肝脏弥漫性疾病诊断中的应用价值

目的:探讨肝脏常见弥漫性疾病的ADC值及DWI上信号强度与相应血供相关性。方法:共110例(19例肝硬化癌变结节、20例肝转移性肿瘤、35例海绵状血管瘤和36例肝囊肿)弥漫性肝占位进行磁共振扫描并计算感兴趣区ADC值、信号强度及增强率。结果:癌变结节与DN及正常肝组织间ADC值相比明显高于后两者,P<0.05。肝囊肿、海绵状血管瘤、肝硬化癌变结节及转移性肝癌在DWI上均为明显高信号。结论:DWI及ADC值对于鉴别弥漫分布的肝脏恶性实质肿块与肝硬化再生结节及良性富水肿块(血管瘤及囊肿)有重要的诊断价值,尤其是在弥漫分布的肝硬化再生结节中发现癌变结节,有其重大的临床意义。     

前列舒颗粒剂的制备及临床疗效观察

本文用丹参、红花等中药材经传统的颗粒剂制备方法,制成前列舒颗粒剂。对处方中的主药红花及赤芍进行质量控制,经小鼠体表消肿实验表明,本品对小鼠背部肿胀有显著的消肿作用。临床应用本品与前列康片及复方磺胺甲恶唑片组比较,无显著性差异。     

成骨细胞在蛋白石/聚左旋乳酸复合材料膜上生长的研究

目的 寻找新型生物医学支架材料.方法 将能产生负离子的蛋白石粉末与常用的生物材料聚左旋乳酸(poly-L-lactic acid,PLLA)制成复合材料膜,在此膜上接种成骨细胞,采用扫描电镜和四甲基偶氮唑盐[3-(4,5-dimethyltyiazol-2-yl)-2,5-diphenyl tetrazolium bromide,MTT]法研究成骨细胞生长与增殖的情况.结果 扫描电镜研究发现复合材料膜比PLLA膜更适合于成骨细胞的生长与增殖,MTT检测进一步证明了这一结果.研究认为蛋白石产生的负离子对细胞的生长与增殖有刺激促进作用.进而探讨了负离子对离体细胞作用的机理.结论 成骨细胞在蛋白石/聚左旋乳酸复合材料上的生长状况良好,蛋白石释出的负离子对细胞的生长与增殖有促进作用.这一研究为开发新的医用支架材料提供了可能.