电针足三里和昆仑对佐剂性关节炎大鼠的治疗作用

目的：建立佐剂性类风湿关节炎大鼠模型,研究电针刺激＂足三里＂和＂昆仑＂穴对佐剂性关节炎大鼠的治疗作用,为针灸治疗风湿性关节炎提供理论依据。方法：在大鼠右后足掌面由掌腱膜向踝关节处皮下注入0.1 mL弗氏完全佐剂,诱发佐剂性关节炎大鼠模型。并用0.25 mm×40 mm针灸针刺入大鼠一侧＂足三里＂和＂昆仑＂穴,深度为5 mm,并连接HANS电针仪进行电针治疗,调节电针仪频率2 Hz,强度2 mA,每天1次,每次30min,连续针灸28d后,观察不同组大鼠关节炎指数和一般状态并测量足趾肿胀度和踝关节病理形态学变化。结果：大鼠注射弗氏完全佐剂后,足跖肿胀度比空白组明显增加,说明已成功建立佐剂性类风湿关节类大鼠模型。电针治疗14d以内,治疗组和模型组大鼠足跖肿胀度无显著差异,但治疗28d后,治疗组大鼠足跖肿胀度和关节症状评分降低,足爪组织炎症情况有所减轻。此外,大鼠注射弗氏完全佐剂及电针治疗不会影响大鼠正常发育。结论：电针刺激＂足三里＂和＂昆仑＂穴对佐剂性关节炎具有较好的疗效。     

针刺大鼠"太溪" 对穴位组织双向电泳图谱影响的实验研究

研究"太溪"穴位组织蛋白质特异性,探寻针刺对大鼠"太溪"穴位组织双向电泳图谱的影响.8周龄雄性Wistar大鼠12只,随机分为空白对照组(n=6)和针刺组(n=6).针刺组每日定时电针双侧"太溪"穴,空白对照组大鼠不予针刺.1周后切取"太溪"穴组织和非经非穴组织,进行双向电泳,比较分析"太溪"穴和非穴组织、"太溪"穴组织针刺前后的双向电泳图谱.大鼠"太溪"穴组织与非经非穴组织存在8个完全差异蛋白点,针刺前后"太溪"穴组织胶图中蛋白点分布发生显著变化,同时可见蛋白点数量由285升至326.说明:"太溪"穴位组织较非穴位组织存在蛋白质差异性,针刺可引起"太溪"穴区组织蛋白质组发生显著变化.     

早期留守经历对大学生人格的影响

目的探讨留守经历在人格形成过程中的影响及作用。方法采用《Temperament and Character Inventory》(TCI)及自编调查表对四川省内1500名大学生进行随机调查。结果①对于大学生来说,有无早期留守经历的大学生在人格的7个维度上无显著差异(t=0.31～1.21,P>0.05);②对于有留守经历的大学生来说,留守经历对人格有明显的影响:父母亲外出打工时孩子的年龄越小,其成人后的自我指向这一维度的得分越高(r父=-0.105,P<0.05;r母=-0.117,P<0.05);母亲外出打工时孩子的年龄越小,协调性的得分越高(r=-0.135,P<0.05);父亲在外打工的时间越长,孩子成人后追求刺激性的人格特征越弱(r=-0.132,P<0.05)。结论早期的留守经历对人格形成有显著的影响。     

Identification of "papillary-like" clusters on endometrial cytologic preparations

The presence of papillary cell clusters is taken as a serious indication in the diagnosis of well-differentiated endometrial adenocarcinoma in Japan. However, papillary-like clusters have been observed in both normal and benign samples. Therefore, overdiagnosis may occur in the observation of cytopreparations. The present study attempts to prepare a histological sample from an Auto Cyto Fix (ACF) sample prepared by the automatic fixation apparatus (ACF1000) using a membrane filter method after cytological observation and to examine its usefulness. In an ACF sample, a papillary-like cluster was observed and the coverslip was then removed, the circumference of the membrane filter was cut off, and the target cell cluster was smeared, embedded, and sliced. In the cases in which a papillary-like cluster was observed, even if differential diagnosis of the derivation was difficult due to a resemblance between the respective morphological findings, it was easily made by histological observation of an ACF sample.     

Direction-dependent effects of chronic "jet-lag" on hippocampal neurogenesis

Disruptions in circadian rhythms, as seen in human shift workers, are often associated with many health consequences including impairments in cognitive functions. However, the mechanisms underlying these affects are not well understood. The objective of the present study is to explore the effects of circadian disruption on hippocampal neurogenesis, which has been implicated in learning and memory and could serve as a potential pathway mediating the cognitive consequences associated with rhythm disruption. Circadian rhythm disruptions were introduced using a weekly 6 h phase shifting paradigm, in which male Wistar rats were subjected to either 6 h phase advances (i.e. traveling eastbound from New York to Paris) or 6 h phase delays (i.e. traveling westbound from Paris to New York) in their light/dark schedule every week. The effects of chronic phase shifts on hippocampal neurogenesis were assessed using doublecortin (DCX), a microtubule binding protein expressed in immature neurons. The results revealed that chronic disruption in circadian rhythms inhibits hippocampal neurogenesis, and the degree of reduction in neurogenesis depends upon the direction and duration of the shifts. In two cohorts of animals that experienced phase shifts for either 4 or 8 weeks, a greater decrease in neurogenesis was observed when the phase was advanced versus delayed in both groups. The direction-dependent effect mirrors the findings on clock gene expression in the SCN, suggesting a causal link between the reduction in hippocampal neurogenesis and a disrupted SCN circadian clock.