子宫内膜异位症不孕妇女腹腔液前列腺素水平

本文测定子宫内膜异位症不孕妇女23例(异位症组)、不明原因不孕妇女11例(不孕对照组)和正常妇女11例(正常对照组)腹腔液前列腺素水平,结果提示:异位症不孕妇女腹腔液中,PGE_2、PGF_(2α)、TXB_2、6-Keto-PGF_(1α)的浓度均高于正常对照组;PGE_2、PGF_(2α)、6-Keto-PGF_(1α)的浓度也高于不孕对照组。四种前列腺素,不孕对照组与正常对照组无区别。表明:腹腔液中前列腺素水平增高可能与异位症引起不孕的机制有关,而不孕对照组的不孕可能是前列腺素以外的其它原因所致。     

绝育术后腹痛患者腹腔液中组织胺水平的测定

绝育术后腹痛患者腹腔液中组织胺水平的测定于俊荣樊瑞琴王振海输卵管绝育术后，有少数患者主诉腹部疼痛。本研究测定１９例输卵管绝育术后严重慢性腹痛患者的腹腔液中组织胺水平，并与健康妇女作对照，探讨组织胺水平变化与绝育术后腹痛的关系。一、资料与方法１．研究对...     

甲地孕酮硅橡胶阴道环对猕猴血浆前列腺素(PGs)含量变化的影响

<正> 本文的目的在于探讨甲地孕酮硅橡胶环,(简称甲孕环)与未含药的空白环对母猴血中某些PGs(PGE_2、PGF_(2α)、6-酮PGF_(1α)及TXB_2)的影响以及它们与宫内出血的可能性联系。合成的高分子材料硅橡胶,由于具有较好的生物稳定性及药物渗透性,因而受到生殖与避孕科技领域的注意。在国际性研究成就的基础上,我国在研制甲地孕酮硅橡胶环的过程中,又进一步对其环型、规格、药物释放量控制等诸环节进行过研究改进,同时对实验与临床应用方面,对避孕效果及安全性的检测与观察均获得了不少成果。但是药环对雌性机体的内环境及其引起的其它激素的相     

输卵管硅橡胶塞绝育术受术者的生殖激素水平

为了研究输卵管硅橡胶塞绝育术对女性生殖内分泌功能的影响,本研究测定了138例健康对照者和76例受术者血清中的6种生殖激素:FSH、LH、E_2、P、T、PRL的水平.结果发现,除PRL外,无论是月经正常的受术者,还是哺乳期闭经的受术者,其FSH、LH、E_2、P的水平与正常对照组相比较均无显著性差异.本研究表明,输卵管硅橡胶塞绝育术对女性生殖内分泌功能的影响不大.受术者PRL的升高只是暂时性的.     

人类黄体溶解中儿茶酚胺和前列腺素F_(2α)的相互作用

在许多动物种属,已证明前列腺素 F_(2α)(PGF_(2α)具有溶黄体作用。最近发现,对于人黄体的甾体生成,PGF_(2α)具有抗促性腺激素作用。据此推测,PGF_(2α)在人类的离体黄体极可能有溶黄体作用,因为要维持人黄体的正常机能必需有 LH 的支持。值得注意的是,PGF_(2α)的抗促性腺激素作用仅在黄体期中期(7～11天)的黄体有效,而对早期黄体(1～3天)则无效。实验证明,人黄体的前列腺素F_(2α)含量及产生以及人黄体的 PGF_(2α)受体含量,在黄体期不同阶段并无差异。然而,早期人黄体的内源性儿茶酚胺含量似乎低于中期黄体。作者     

月经周期排卵的年轻妇女和绝经后妇女尿中PGs的测定

为探讨月经周期中未孕排卵年轻妇女和绝经 后妇女前列腺素(PG)的分泌及女性激素雌二醇(E_2)和孕酮(P)对尿中PG类排泄的影响,测定41例处于卵泡期和黄体期的年轻、健康、排卵、未孕妇女(18～40岁)和23例健康绝经后妇女(<65岁)主要PG类(PGF_2,6-酮-PGF_1α,TXB_2及其代谢产物PGE-M,2,3-二去甲-6-酮-PGF_1 α,2,3-二去甲-TXB_2和11-脱氢-TXB_2)的尿排泄率(自然尿样)。同时测定了相应时间血中的E_2和P。年轻受试者需两次     

实验研究

001 外原性PGE_2对促性腺激素诱发的未成熟大鼠排卵的抑制作用[英]/Espey LL…//Prostaglandins.-1992,43(1).-67～74 前列腺素类(PGs)是排卵过程中的重要介质。但前列腺素E_2(PGE_2)和前列腺素F_(2a)(PGF_(2a))     

黄体酮和17-β E_2对卵巢切除术羊的催产素诱导的PGF_(2α)释放及对子宫内膜催产素受体浓度的影响

催产素、黄体酮及E_2是调节子宫PGF_(2α)分泌的重要激素。为研究黄体酮和E_2对切除卵巢羊的催产素诱导的PGF_(2α)释放及对子宫内膜催产素受体的影响。实验1中,用16只动情间期杂种的羊,卵巢切除1个月后给予6天激素处理,模仿发情期前激素变化,前4天肌注黄体酮,后3天肌注E_2,第7天定为模拟周期第0天。随机分4组①对照组;②黄体酮     

性激素对兔输卵管、子宫组织前列腺素(PGS)含量影响的研究

利用性激素加速或减慢卵子运行,造成输卵管峡部功能闭锁或松弛,观察兔输卵管、子宫组织PGE_1、PGF_(2α)及E/F比值的变化。实验分对照组、孕激素(MQG)组和雌激素(ECP)组。1.三组各部组织PGE_1、PGF_(2α)含量比较:壶-峡交接部、远峡部、近峡部含量最高,子宫次之,壶腹部最低。2.孕激素(MQG)和雌激素(ECP)均能使兔交配后28小时输卵管PGE_1、PGF_(2α)升高,孕激素使PGE_1上升的程度较大,而雌激素使PGF_(2α)上升的程度较大。E/F比值:对照组E/F接近1,孕激素组E/F大于1,雌激素组E/F小于1。3.三组兔交配后28小时与76小时峡部PGE_1、PGF_(2α)含量变化比较:对照组,PGE_1无明显变化,PGF_(2α)则76小时比28小时降低40～50%(76小时约有20%卵进入子宫);孕激素组,PGF_(2α)无明显变化,PGE_128小时比对照组及雌激素组高(28小时有75%卵比对照组提前二天进入子宫,峡部收缩频率低),76小时壶-峡交接部、远峡部比28小时降低约40%,接近对照组28小时PGE_1水平;雌激素组,PGE_176小时远峡、近峡部比28小时降20～40%,而PGF_(2α)在28小时较高水平上远峡部又继续上升15%(此时卵全部停留在输卵管内,比对照组延迟2天进入子宫,峡部收缩频率高)。两种不同类型激素激发兔输卵管前列腺素含量的变化,在时间上符合两种不同激素对兔卵子运行和输卵管峡部收缩频率产生的相反效应,这一结果似乎显示输卵管闭锁功能与卵子运行之间的关系不仅与PGS的量有关,而且与PGE_1、PGF_(2α)的比值有关,两者关系可能通过PGE和PGF平衡调节。     

雷夫奴尔流产和引产时羊水中的前列腺素和凝血氧环

作者对15名用雷夫奴尔羊膜腔外引产的妊娠妇女进行了研究,其中12例中期妊娠,2例死胎,1例无脑儿。本研究目的是要了解用雷夫奴尔引产时羊水中的游离花生四烯酸(AA)、前列腺素F_(2α)(P-GF_(2α))、前列腺素E_2(PGE_2)、6-酮-前列腺素F_(1α) (6-酮-PGF_(2α))和凝血氧环B_2(TXB_2)的浓度。经腹壁羊膜腔穿刺,每隔1～4小时抽取一次羊水标本,直到48小时为止。用气液层析法测定羊水中的AA浓度,用放射免疫法测定羊水中的PGF_(2α)、PGE_2、TXB_2和6-酮-PGF_(1α)的浓度。引产的方法是羊膜腔外滴注0.1%的雷夫奴尔溶液150ml。在引产期间无并发症发生。中期妊娠的平均引产一流     

环氧合酶-2和前列腺素类物质在子宫内膜异位症的表达研究

目的:研究环氧合酶-2(Cox-2)、6-酮-前列腺素F1α(6-k-PGF1α)和血栓素B2(TXB2)与子宫内膜异位症(EMs)的发生及临床病理因素的关系。方法:采用免疫组织化学SABC法检测45例EMs和30例正常子宫内膜Cox-2蛋白的表达;采用放射免疫法检测组织液6-k-PGF1α和TXB2的含量。结果:Cox-2在腺上皮的胞浆中表达,EMs组Cox-2表达明显高于对照组(P<0.001);EMs的AFS分期、疼痛分级以及瘤体大小之间Cox-2表达差异无统计学意义(P>0.05)。EMs组TXB2和6-k-PGF1α的含量明显高于对照组(P<0.001);有症状组6-k-PGF1α的含量高于无症状组,差异有统计学意义(P<0.05);TXB2含量两组无差别。Cox-2蛋白的表达与6-k-PGF1α和TXB2的含量相关。结论:Cox-2、6-k-PGF1α及TXB2的高表达可能与EMs的发生相关。6-k-PGF1α可能与痛经相关。     

Eicosanoids in primary dysmenorrhea, endometriosis and menstrual migraine

This paper summarizes what has been learned over the years about the role of eicosanoids in the pathogenesis of primary dysmenorrhea, endometriosis and menstrual migraine. The role of prostaglandins (PGs) in the pathogenesis of primary dysmenorrhea is inferred from four main observations: firstly, the clinical symptoms of primary dysmenorrhea are similar to those induced by the administration of PGF2 alpha and PGE2 for the induction of labour; secondly, the increased production of PGs by the endometrium during the luteal and menstrual phases of ovulatory cycles is consistent with the occurrence of primary dysmenorrhea mainly in ovulatory cycles; thirdly, the concentrations of PGF2 alpha and PGE2 in the endometrium and menstrual fluid of dysmenorrheic women are significantly higher than in controls; fourthly, certain PG inhibitors have been proved to be effective in the treatment of dysmenorrhea. The change in PG production can explain the major symptoms of primary dysmenorrhea, including the increased uterine contractility, uterine ischemia and the lowering of the pain threshold to chemical and physical stimuli in the pelvic nerve terminals. Moreover, recent experimental data suggest that leukotrienes (LTs) might be among the alternative pathogenetic causes of primary dysmenorrhea. The data which support a relationship between eicosanoids and endometriosis are as follows: endometriotic tissue produces PGs; the peritoneal fluid concentration of PGF2 alpha increases significantly after the induction of endometriosis in laboratory animals; the concentration of PGs in peritoneal fluid of some patients with endometriosis is greater than in controls and, finally, the number and activation of pelvic macrophages which are able to synthesize eicosanoids increase in patients with endometriosis. Possible roles for eicosanoids in the pathogenesis of infertility and secondary dysmenorrhea induced by endometriosis have been suggested. Eicosanoids are probably also involved in the pathogenesis of menstrual migraine. Different types of PGs might play a role both in the initial vasoconstriction during the prodromal phase of migraine and in the vasodilation and sensitization to pain typical of the pain phase.     

米非司酮对早孕妇女血浆生殖激素及蜕膜和绒毛组织中前列腺素水平的影响

本文用放免法测定了妊娠35～67d妇女口服米非司酮100mg前和48h后血浆中E2、P、PRL、βhCG水平和妊娠40～56d口服米非司酮48h后蜕膜和绒毛组织中的PG（2α）、PGE2、TXB2和6－keto－PGF（1α）水平的变化。结果显示米非司酮使血浆P水平降低（P＜0．01）；PRL水平增加（P＜0．01）；E。在用药后48h继续呈上升趋势，但与用药前无显著统计学差异；E2／P和PRL／P比值增力。（P＜0．05，P＜0．01）。6－keto－PGF（1α）在用药后有腹痛和／或子宫出血受试者蜕膜组织中的水平明显高于无症状受试者和对照组；绒毛组织中PGF（2α）、TXB2和6－keto－PGF（1α）水平在用药后有腹痛和／或子宫出血者和无症状受试者都明显高于对照组（P＜0．05，P＜0．005），PGF（2α）／PGE2比值也明显增加（P＜0．05）。结果显示了来非司酮对早孕妇女的综合影响，包括改变内分泌激素之间和PGS之间比率的平衡，改变内分泌激素和PGs的浓度，促使子宫收缩及宫颈软化等。     

Peritoneal fluid interleukin-6 in women with chronic pelvic pain

OBJECTIVE: To determine the relationship between peritoneal fluid concentrations of interleukin-6 (IL-6) and chronic pelvic pain symptomatology in women with adhesions, endometriosis, or no obvious intraperitoneal pathology. DESIGN: Clinical research study. SETTING: Healthy volunteers in an academic research environment. PATIENT(S): Reproductive-aged women undergoing laparoscopy for the diagnosis of pelvic pain, infertility, or sterilization were selected. INTERVENTION(S): Peritoneal fluid was collected at the time of the laparoscopy and later assayed for IL-6. Subjects completed a pelvic pain questionnaire, and operative reports were used to obtain the underlying diagnosis. MAIN OUTCOME MEASURE(S): Interleukin-6 concentrations. RESULT(S): No correlation between the presence or absence of pelvic pain, findings of adhesions or endometriosis, and the concentration of peritoneal fluid IL-6 was observed. Conclusion(s): The cytokine IL-6 does not seem to play a role in the genesis of chronic pelvic pain in women with adhesions or endometriosis.     

人体蜕膜释放前列腺素的研究

本文测定了10例体外培养人体蜕膜前列腺素的释放,并观察了芫花萜对蜕膜细胞的作用。实验证明,体外培养近一周的人体蜕膜细胞有合成PGE_1和PGF_(2α)的能力。芫花萜对蜕膜细胞的作用,主要是引起溶酶体膜通透性的改变。     

Association of leptin with inflammatory cytokines and lymphocyte subpopulations in peritoneal fluid of patients with endometriosis

INTRODUCTION: Endometriosis is a common, complex and chronic disease related to ectopic implantation and growth of endometrial tissue that may manifest by pelvic inflammatory reactions, chronic pelvic pain and subfertility. Endometriosis may be associated with increased peritoneal fluid leptin levels. Leptin is known to exert immunomodulatory effects; however, an association between leptin and inflammatory reactions in endometriosis has not been documented. Therefore, the aim of this study was to investigate a relationship between leptin concentrations in peritoneal fluid and the levels of peritoneal fluid inflammatory cytokines and mononuclear leukocyte subpopulations. MATERIALS AND METHODS: Peritoneal fluid was aspirated by laparoscopy from 46 women in whom endometriosis had been confirmed by clinical and histopathological examinations and from 10 control women qualified for ART in whom pelvic pathology has been excluded. Concentrations of leptin and inflammatory cytokines (IL-1beta, IL-6, IFN-gamma and TNF) in peritoneal fluid were evaluated by specific ELISAs. Percentage of peritoneal leukocyte subpopulations (CD3+, CD4+, CD8+ and CD14+) was analyzed by FACS using specific monoclonal antibodies. RESULTS: Leptin concentrations in peritoneal fluid correlated negatively with concentrations of IL-1beta and IFN-gamma (r(s)=-0.38, p=0.01 and r(s)=-0.31, p=0.03, respectively) and correlated positively with the percentage of CD3+ pan-T cells (r(s)=0.69, p=0.009) and CD4+ T helper cells (r(s)=0.74, p=0.036). CONCLUSIONS: Increased leptin levels in peritoneal fluid from endometriosis patients may affect local inflammatory/immune reactions, especially infiltration of CD4+ T helper cells. Thus, leptin may play an important role in the immunopathogenesis of endometriosis.     

A relationship between increased peritoneal leptin levels and infertility in endometriosis

Endometriosis is a common, complex and chronic disease related to ectopic implantation and growth of endometrial tissue that may manifest by pelvic pain, and accounts for over 20% of all cases of infertility in women. Endometriosis may be associated with increased levels of leptin in peritoneal fluid. However, the association of leptin with infertility has not been definitely documented. Therefore, the aim of the present study was to search for a relationship between concentrations of peritoneal-fluid leptin and patients' clinical status. The study included 56 patients being diagnosed for infertility and/or pelvic pain. Peritoneal fluid was aspirated during routine laparoscopic examination. Concentrations of leptin in peritoneal fluid were evaluated by a specific enzyme-linked immunosorbent assay. The results revealed that the levels of peritoneal-fluid leptin did not correlate with different stage of endometriosis. However, they correlated with body mass index. Leptin levels were significantly higher in infertile patients than in patients with pelvic pain (p = 0.0023 by Mann–Whitney U test or p = 0.0045 by analysis of variance). It may suggest that increased leptin levels in the peritoneal fluid may play a role in pathogenesis of infertility.     

彩色多普勒监测妊高征孕妇子宫胎盘血流变化

应用彩色多普勒血流显像技术监测了３１例妊高征孕妇及７４例正常孕妇子宫胎盘血流，同时测定血中雌三醇、胎盘泌乳素、血栓素代谢产物、前列环素代谢产物及ＴＸＢ２／６ＫＰ比值。结果表明：正常孕妇子宫动脉及胎儿脐动脉的时间平均血流速度及血流量明显高于妊高征孕妇。