细胞色素P450 2E1（CYP2E1）的遗传多态性与非吸烟女性肺癌的 …

我们在上海和哈尔滨二地分别收集了非吸烟女性肺癌病例各１００例和８２例以及非吸烟的女性健康人对照各９５例和８９例，分析了白细胞ＤＮＡ的细胞色素Ｐ４５０２Ｅ１基因第六内含子ＤｒａＩ切点和５’端调控区ＲｓａＩ切点的遗传多态性。虽然上海病例组ＤｒａＩ和ＲｓａＩ突变型纯合子与对照组比ＯＲ值分别为２．５６和５．４７，但未在显著性；哈尔滨病例组ＯＲ值分别为０．４９和０．４７，无显著差异。     

非吸烟女性肺癌患者细胞色素P4501A1和GSTM1的基因型

目的分析非吸烟肺癌女性与健康女性的细胞色素Ｐ４５０１Ａ１和ＧＳＴＭ１的基因型。方法比较上海和哈尔滨二地的非吸烟肺癌女性各１００例和８２例，以及年龄配对的健康女性各９５和８９例。用酚┐氯仿法提取白细胞中的ＤＮＡ，分析细胞色素Ｐ４５０１Ａ１和ＧＳＴＭ１的基因型。结果两组基因型在病例和对照间以及两地间无差异。结论虽然在上海人群中ＣＹＰ１Ａ１突变型纯合子合并ＧＳＴＭ１缺失的ＯＲ值可达６．５９，但未达统计学显著性     

非吸烟女性肺癌患者Apo E的基因多态性

为了探讨载脂蛋白Ｅ（ａｐｏＥ）的遗传变异体与非吸烟女性肺癌易感性之间的关系，在上海市和哈尔滨市两地分别收集了非吸烟女性肺癌病例１００例和８２例，以及非吸烟的女性健康人对照９５例和８９例，应用ＰＣＲ－内切酶片段长度多态性技术（ＰＣＲ－ＲＦＬＰ），分析了白细胞ＤＮＡａｐｏＥ等位基因（ａｐｏ∈）的遗传多态性。结果显示，把突变型等位基因∈４与野生型∈３相比，虽然在上海市病例组中，ＯＲ值为０．５２（９５％ＣＩ＝０．２１～１．２９），但无显著性差异，尚待扩大样本做进一步探讨。     

评价酶联免疫检测血清CYFRA21—1对非小细胞肺癌的诊断价值

目的评价检测ＣＹＦＲＡ２１┐１对非小细胞肺癌的诊断价值。方法用ＥＬＩＳＡ法测定７０例肺癌（ＬＣ）其中４７例非小细胞肺癌（ＮＳＣＬＣ），３例小细胞肺癌（ＳＣＬＣ）和２０例未分型肺癌、６４例肺部良性疾病患者及４０例健康人血清ＣＹＦＲＡ２１┐１浓度。试验的诊断性能用相对操作特征（ＲＯＣ）分析法估测之。结果测得全阈诊断准确率（ＯｖｅｒａｌＤｉａｇ┐ｎｏｓｔｉｃＡｃｃｕｒａｃｉｅｓ）ＬＣ为０．７５、ＮＳＣＬＣ为０．７６，ＳＱＣ为０．８３，ＡＤＥ为０．６７和ＳＣＬＣ为０．３８。在相应于特异性为０．９５的界定值３．４７μｇ／Ｌ处，各型的灵敏度分别为ＳＱＣ０．６２，ＬＣ０．５３，ＮＳＣＬＣ０．５１，ＡＤＥ０．４８和ＳＣＬＣ０．００。结论结果显示ＣＹＦＲＡ２１┐１是ＮＳＣＬＣ较灵敏和特异的一个标志物。未观察到ＴＮＭ各期间该标志物的平均水平有明显的差异；然而异常升高水平的患者的比例随病期的进展而显著增加，提示一系列检测ＣＹＦＲＡ２１┐１水平可能有助于监查ＮＳＣＬＣ患者的疗效。     

女性乳腺癌危险因素的Logistic回归分析

女性乳腺癌危险因素研究资料采集于广州，韶关，肇庆等八个医院。按１∶２配对设计总共收集了１２５个病例一对照组作回顾性调查。经条件Ｌｏｇｉｓｔｉｃ回归．分析得：保护因子有喝茶习惯，ＯＲ＝０．３９１３；危险因子有精神创伤，ＯＲ＝３．４３３８，乳腺炎史，ＯＲ＝３．４０９９，被动吸烟，ＯＲ＝２．０７９６，平均摄油量，ＯＲ＝３．１７７３，流产，ＯＲ＝１．３５４４，癌症家族史，ＯＲ＝５．６７９７。     

儿童急性白血病的有效化学治疗

目的总结了我院初治１１５例急性淋巴细胞白血病（ＡＬＬ）及３０例急性髓性白血病（ＡＭＬ）的诱导缓解治疗效果,同时介绍了早期巩固强化治疗方案。方法ＡＬＬ诱导化疗方案：病人共分三组：（１）ＣＯＰ,（２）ＣＯＰ＋ＤＮＲ,（３）ＣＯＰ＋ＩＤＡ。以第８天骨髓象＜５％为良好快速早期反应（ｒａｐｉｄｅａｒｌｙｒｅｓｐｏｎｓｅ,ＲＥＲ）的标准。ＡＭＬ诱导缓解治疗方案为ＡＤ＋ＶＰ１６。结果三组ＲＥＲ良好的百分率分别为：６８３％,７５７％,９０９％。第一组与第三组之间Ｐ＜００５,疗效存在显著性差异。ＡＭＬ一个疗程达到完全缓解的占９０％。结论可供分析无病生存期的ＡＬＬ病例共６０例,无病生存达４年以上者占８５％（５１／６０）。ＡＭＬ共３０例,无病生存达４年以上者占７０％（２１／３０）。     

重庆市妇女乳腺癌危险因素的病理对照研究

目的 研究女性乳腺癌的危险因素。方法 在重庆市进行了首次１：１流行病学病例对照研究。结果 多因素条件Ｌｏｇｉｓｔｉｃ回归分析显示了下列因素对乳腺癌的影响：女性初潮年龄（ＯＲ＝０．７９，９５％ＣＩ＝０．６７－０．９２），月经紊乱史（ＯＲ＝１．４７，９５％，ＣＩ＝１．０７－２．０２），高龄初产（ＯＲ＝１．５５，９５％ＣＩ＝１．２０－１．９８），乳腺良性疾病史（ＯＲ＝２．３３，９５％ＣＩ＝１．０２－５．     

肝素、5-羟色胺和白介素-1对NIH/3T3成纤维细胞AgNORs影响的图像分析

应用核仁组成区相关蛋白银染技术（ＡｇＮＯＲｓ）结合图像分析观察了肝素、５－羟色胺和白介素质（ＩＬ－１）对体外培养的ＮＩＨ３Ｔ３成纤维细胞ＡｇＮＯＲｓ的影响。结果表明：（１）加入０．１－０．４ｍｇＬ浓度的肝素培养后第３天，成纤维细胞嗜银蛋白颗粒面积（Ａｇ－ａ）、积分光密度（Ａｇ－ｉｏｄ）和嗜银相关蛋白面积与核面积的百分比（Ａｇ－ａ／Ｎａ）均出现升高，其中以０．１０和０．２０ｍｇＬ浓度的肝素刺激作用最强，三个指标的均值分别为对照值的１．４７、１．５４和１．９４倍（Ｐ＜０．０１）；（２）加入不同浓度５－羟色胺后ＡｇＮＯＲｓ的三个参数呈现出类似的升高趋势，而以１．０和２．０×１０－６ｍｏｌＬ浓度的５羟色胺显示出较强的促进作用，成纤维细胞ＡｇＮＯＲｓ的三个指标相当于对照值的１．５３、１．５７和２．４２倍；（３）在所观察的ｌＬ－１四种浓度范围内，以１００和２００×１０３ｕＬ浓度的ｌＬ－１对成纤维细胞的促增殖作用最强，三个指标的均值分别为对照值的１．６０、１．８４和２．４２倍。     

饮茶与女性肺癌关系的流行病学研究

调查研究饮茶与女性肺癌的关系。方法用全人群病例对照研究方法，调查６４９例３５～６９岁女性肺癌新病例，按频数配对随机配以６７５名对照。结果饮茶对非吸烟者肺癌有保护作用，ＯＲ＝０．６３，９５％置信区间＝０．４６～０．８７，ＯＲ值还随饮茶量增加而降低（趋势检验Ｐ＝０．００２７）。饮茶对各种组织类型的非吸烟者肺癌都有不同程度的保护作用。在吸烟者中，由于吸烟因素本身的干扰，未见饮茶的保护作用。结论饮茶对肺癌的保护作用值得进一步研究。     

Ｏ-ＭＡＲ联合 ｉＤｏｓｅ４ 技术在改善宫颈癌后装治疗ＣＴ定位图像金属施源器伪影中的应用

目的：探讨金属伪影去除技术（ＭｅｔａｌＡｒｔｉｆａｃｔＲｅｄｕｃｔｉｏｎｆｏｒＯｒｔｈｏｐｅｄｉｃＩｍｐｌａｎｔｓ,Ｏ-ＭＡＲ）联合 ｉＤｏｓｅ４ 技术在三维后装ＣＴ图像中去除金属施源器伪影的应用价值。方法：回顾性收集行三维后装 ＣＴ定位的宫颈癌患者 ３３例, 每位患者行 ５～６次近距离后装治疗,每次治疗前均采用ＣＴ进行施源器的定位,同时选择Ｏ-ＭＡＲ＆ｉＤｏｓｅ４、Ｏ-ＭＡＲ、 ｉＤｏｓｅ４和标准（Ｓｔａｎｄａｒｄ）４种算法进行图像的重建,每次扫描获得 ４组重建图像。计算各组图像标准差（ｓｔａｎｄａｒｄｄｅ ｖｉａｔｉｏｎ,ＳＤ）代表噪声值、伪影指数（ａｒｔｉｆａｃｔｉｎｄｅｘ,ＡＩ）和对比噪声比（ｃｏｎｔｒａｓｔｎｏｉｓｅｒａｔｉｏ,ＣＮＲ）,进行客观分析,同时由 ２名放疗医师采用盲法五分制法对 ４组图像进行主观评分。结果：四种重建算法产生的 ＳＤ（Ｈ＝１８３．０１２,Ｐ＜ ０．００１）、ＣＮＲ（Ｈ＝２８．７０６,Ｐ＜０．００１）和 ＡＩ（Ｈ＝１８１．４３０,Ｐ＜０．００１）的差异均有统计学意义,其中 Ｏ-ＭＡＲ＆ｉＤｏｓｅ４ 组的 ＳＤ１６．５５（１２．５３～１９．４０）和 ＡＩ１４．３４（１０．２９～１８．２５）显著低于其余三组（Ｐ＜０．００１）,ＣＮＲ０．０４（０．０１～０．０８） 显著大于其余三组（Ｐ＜０．００１）;主观评分上 Ｏ-ＭＡＲ＆ｉＤｏｓｅ４组与 Ｏ-ＭＡＲ组的评分均为 ５（４～５）,显著高于其余两 组（Ｐ＜０．００１）。结论：在三维后装模拟定位中,Ｏ-ＭＡＲ联合 ｉＤｏｓｅ４算法可减少金属施源器伪影,增加放疗医生勾画 的信心和准确性。     

CYP2E1 PstI/RsaI polymorphism and interaction with alcohol consumption in hepatocellular carcinoma susceptibility: evidence from 1,661 cases and 2,317 controls

Many studies have suggested that cytochrome P450 2E1 (CYP2E1) gene might be involved in the development of hepatocellular carcinoma (HCC). However, the results have been inconsistent. In this study, the authors performed a meta-analysis to clarify the association between Pst I/Rsa polymorphism in the CYP2E1 gene and HCC risk. PubMed and China National Knowledge Infrastructure were searched for eligible publications. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using fixed- or random-effects model. Fifteen studies (1,661 HCC cases and 2,317 controls) were identified for the data analysis. The overall result showed that there was no statistically significant association between CYP2E1 Pst I/Rsa polymorphism and HCC risk (c2/c2 vs. c1/c1, OR = 0.73, 95% CI 0.50-1.06; c1/c2 vs. c1/c1, OR = 1.00, 95% CI 0.76-1.33; c2/c2+ c1/c2 vs. c1/c1, OR = 0.99, 95% CI 0.77-1.26; c2/c2 vs. c1/c2+ c1/c1, OR = 0.73, 95% CI 0.50-1.06). Further stratified analyses indicated that the habitual alcohol drinkers with c2 alleles were more likely to develop HCC (OR = 1.73, 95% CI 1.19-2.51), compared with the non-habitual drinkers with c1 homozygote. The meta-analysis indicated that CYP2E1 Pst I/Rsa polymorphism was not associated with HCC risk, while the interaction between Pst I/Rsa polymorphism and alcohol consumption increased the risk of HCC.     

CYP2E1 polymorphisms and colorectal cancer risk: a HuGE systematic review and meta-analysis

Studies investigating the associations between Cytochrome P4502E1 (CYP2E1) polymorphisms and colorectal cancer (CRC) risk report conflicting results. We conducted a meta-analysis to assess the association between CYP2E1 gene Rsa I/Pst I, Dral T/A and 96-bp insertion polymorphisms and CRC susceptibility. Two investigators independently searched the Medline, Embase, CNKI, Wanfang, and Chinese Biomedicine Databases. Summary odds ratios (ORs) and 95 % confidence intervals (95 % CIs) for CYP2E1 polymorphisms and CRC were calculated in a fixed-effect model (the Mantel–Haenszel method) and a random-effects model (the DerSimonian and Laird method) when appropriate. Ultimately, 12, 5, and 4 studies were found to be eligible for meta-analyses of Rsa I/Pst I, Dral T/A, and 96-bp insertion polymorphisms, respectively. Our analysis suggested that the variant genotype of Rsa I/Pst I were associated with a significantly increased CRC risk (c2/c2 vs. c1/c1, OR?=?1.36, 95 % CI?=?1.04–1.77; recessive model, OR?=?1.35, 95 % CI?=?1.04–1.75). Moreover, similar results were observed between CYP2E1 96-bp insertion polymorphism and CRC risk (dominant model, OR?=?1.25, 95 % CI?=?1.07–1.45), while no association was observed between CYP2E1 Dral T/A polymorphism and CRC susceptibility in any genetic model. No publication bias was found in the present study. This meta-analysis shows that CYP2E1 Rsa I/Pst I and 96-bp insertion polymorphisms may be associated with CRC risk. The CYP2E1 Dral T/A polymorphism was not detected to be related to the risk for CRC.     

Association of GSTM1, CYP1A1 and CYP2E1 genetic polymorphisms with susceptibility to lung adenocarcinoma: a case-control study in Chinese population

A case-control study of 164 lung adenocarcinoma (AC) patients with 181 age- and gender-matched healthy controls was conducted in order to assess any associations between glutathione- S -transferase M1 (GSTM1), cytochrome P4501A1 (CYP1A1) and cyto-chrome P4502E1 (CYP2E1) polymorphisms and susceptibility to lung AC in Chinese. The presence of CYP2E1 variant allele was significantly less frequent in cases than in controls, while the distribution of GSTM1 null genotype and variant CYP1A1 Msp 1 allele did not vary between cases and controls. After adjustment for age, gender, smoking and all other genotypes, the CYP2E1 Rsa1 variant allele was significantly associated with decreased risk of lung AC [odds ratio 0.534 (95% confidence interval, 0.340–0.837)]. Furthermore, 3.0-fold increased risk was found in individuals with combined GSTM1 null genotype and CYP2E1 Rsa 1 wild type versus those with combined GSTM1 non-null type and CYP2E1 variant allele. Our results suggest that CYP2E1 Rsa 1 variant allele is associated with a decreased risk of lung AC, and combined GSTM1 null genotype and CYP2E1 Rsa1 wild type has a promoting effect on susceptibility to lung AC. (Cancer Sci 2003; 94: 448–452)     

Genetic polymorphisms of hormone-related genes and prostate cancer risk in the Japanese population

Carcinogenesis of the prostate involves androgen influences, and associations between genetic polymorphisms of androgen receptor and metabolizing enzymes and prostate cancer risk have been reported. Roles for non-androgenic hormones are not well defined, but they also may have an impact judging from epidemiological and animal experimental alphalambda zeta of data. The purpose of the study was to determine whether hormone-related polymorphisms are associated with prostate cancer risk. A case-control study was performed with 147 Japanese prostate cancer patients and 266 urological controls. Polymorphisms of target genes [cytochrome P450 (CYP) 1B1, Leu432Val; debrisoquine hydroxylase, (CYP2D6)*4; aromatase (CYP19), Arg264Cys; estrogen receptor (ER)alpha-Xx (Xba I) and Pp (Pvu II); ERbeta-Rr (Rsa I); progesterone receptor (PR) Alu in intron 7] were examined by PCR-based methods. The capital and small letters signify the absence and presence of restriction sites, respectively. Odds ratios (OR) were adjusted for age using multiple logistic regression analysis with SPSS Medical Pack. Among the seven examined genetic polymorphisms, significant associations between CYP1B1 Leu432Val (OR 4.80; 95% confidence interval (CI), 1.21-19.05) and Alu in intron 7 of PR (OR 4.17; 95%CI, 1.26-13.85) were found. As for combined effects, the CYP1B1 polymorphisms (Leu/Val+Val/Val) together with heterozygosity for Alu in the PR were more frequent among prostate cancer patients (1.45%) than controls (0.41%), although without significance (OR, 3.99; 95%CI, 0.36-44.8). The combination of ERalpha (P/p+p/p) polymorphisms with heterozygosity for Alu in the PR demonstrated an OR of 4.56 (95%CI, 1.01-20.6). This pilot study showed that CYP1B1 and PR polymorphisms, alone or in combination, might be associated with prostate cancer risk. They might, therefore, have potential as a tool for identifying high-risk individuals.     

CYP1A1, CYP2E1 and GSTM1 genetic polymorphisms. The effect of single and combined genotypes on lung cancer susceptibility in Chilean people.

CYP1A1, CYP2E1 and GSTM1 polymorphisms were evaluated in Chilean healthy controls and lung cancer patients. In the Chilean healthy group, frequencies of CYP1A1 variant alleles for MspI (m2 or CYP1A1*2A) and ile/val (val or CYP1A1*2B) polymorphisms were 0.25 and 0.33, respectively. Frequencies of variant alleles C (CYP2E1*6) and c2 (CYP2E1*5B) for CYP2E1 were 0.21 and 0.16, respectively and frequency for GSTM1(-) was 0.24. The presence of variant alleles for GSTM1, MspI and Ile/val polymorphisms was more frequent in cases than in controls. However, frequencies for the c2 and C alleles were not significantly different in controls and in cases. The estimated relative risk for lung cancer associated to a single mutated allele in CYP1A1, CYP2E1 or GSTM1 was 2.41 for m2, 1.69 for val, 1.16 for C, 0.71 for c2 and 2.46 for GSTM1(-). The estimated relative risk was higher for individuals carrying combined CYP1A1 and GSTM1 mutated alleles (m2/val, OR=6.28; m2/GSTM1(-), OR=3.56) and lower in individuals carrying CYP1A1 and CYP2E1 mutated alleles (m2/C, OR=1.39; m2/c2, OR=2.00; val/C, OR=1.45; val/c2, OR=0.48; not significant). The OR values considering smoking were 4.37 for m2, 4.05 for val, 3.47 for GSTM1(-), 7.38 for m2/val and 3.68 for m2/GSTM1(-), higher values than those observed without any stratification by smoking. Taken together, these findings suggest that Chilean people carrying single or combined GSTM1 and CYP1A1 polymorphisms could be more susceptible to lung cancer induced by environmental pollutants such as polycyclic aromatic hydrocarbons.     

细胞色素P450 2E1和谷胱甘肽转硫酶P1基因与食管癌易患性

目的研究与致癌物亚硝胺代谢激活有关的细胞色素Ｐ４５０２Ｅ１基因（ＣＹＰ２Ｅ１）,和与致癌物代谢解毒有关的谷胱甘肽转硫酶Ｐ１基因（ＧＳＴＰ１）多型性与食管癌易患性的关系。方法采用病例－对照分子流行病学方法。以ＰＣＲ－ＲＦＬＰ方法分析食管癌、食管上皮重度增生病例,和与其年龄性别配对的正常对照者（各４５例）ＣＹＰ２Ｅ１和ＧＳＴＰ１的基因型。结果ＧＳＴＰ１基因型在病例和对照者中的分布无显著差别,但ＲｓａＩ识别的ＣＹＰ２Ｅ１基因型,在食管癌、食管上皮重度增生病例及其正常对照者中的分布差别显著。ＣＹＰ２Ｅ１突变型基因频率在正常对照组中为５５．６％,显著高于食管上皮重度增生病例（１７．８％）和食管癌病例（２０．０％;χ２＝２０．８,Ｐ＜０．００１）;携带野生型ＣＹＰ２Ｅ１的个体,发生食管上皮重度增生和食管癌的危险性,比携带变异型ＣＹＰ２Ｅ１的个体各高５倍。结论ＣＹＰ２Ｅ１基因是涉及食管癌变早期过程的遗传易患性因素。     

Hepatitis B and C viruses infection, lifestyle and genetic polymorphisms as risk factors for hepatocellular carcinoma in Haimen, China.

A case-control study was carried out to investigate the impact of factors including virus infection, aflatoxin B1, microcystins, smoking/drinking and dietary habits as well as genetic polymorphisms of aldehyde dehydrogenase 2 (ALDH2) and cytochrome P4502E1 (CYP2E1), on susceptibility to hepatocellular carcinoma (HCC) in Haimen, China. A total of 248 patients with HCC and 248 sex-, age- and residence-matched population-based controls were recruited into the study. Virus infection, and ALDH2 and CYP2E1 gene polymorphisms were assessed in 134 paired cases and controls. By univariate analysis, hepatitis B virus (HBV) infection (odds ratio [OR]=9.75; 95% confidence interval [CI]=4.71-20.2), history of intravenous injection (OR=1.50; 95%CI=1.02-2.22), average income (OR=0.63; 95%CI=0.43-0.92), frequent intake of foods rich in protein, e.g., egg (OR=0.6; 95%CI=0.42-0.87), chicken (OR=0.53; 95%CI=0.35-0.79), pork (OR=0.67; 95%CI=0.46-0.98) and fresh fish (OR=0.58; 95%CI=0.39-0.87) significantly differed between cases and controls. However, peanut intake (OR=0.66; 95%CI=0.43-1.01), source of drinking water, including tap (OR=1.33; 95%CI=0.81-2.20), deep well (OR=0.94; 95%CI=0.56-1.55), shallow well (OR=0.85; 95%CI=0.55=1.30), river (OR=0.95; 95%CI=0.65-1.38), ditch (OR=1.09; 95%CI=0.76-1.55) and pond water (OR=1.0; 95%CI=0.14-7.10) were not significantly associated with risk. Univariate analysis also indicated that the 1-1 genotype of ALDH2 (OR=1.38; 95%CI=0.86-2.23) as well as the Pst1- and Rsa1-digested c1/c1 genotype of CYP2E1 (OR=1.36; 95%CI=0.81-2.28), was slightly more frequent in the case group. On multivariate analysis, HBV infection (OR=13.9; 95%CI=5.78-33.6) and history of intravenous injection (OR=2.72; 95%CI=1.24-6.00) were still associated with significantly increased risk of HCC, while frequent intake of fresh fish (OR=0.32; 95%CI=0.12-0.86) decreased this risk. These findings suggest that whereas peanut intake, water sources as well as genetic polymorphisms in ALDH2 and CYP2E1 do not significantly correlate with the risk of HCC, HBV infection is a main risk factor, and dietary items rich in protein, especially fresh fish, might protect against the risk of HCC in Haimen, China.     

Lack of association of cytochrome P450 2E1 genetic polymorphisms with the risk of human hepatocellular carcinoma

The iso-enzyme pattern of cytochrome P450 was shown to be related to the development of chemically induced hepatocellular carcinoma (HCC) in rats, which is accelerated by chronic alcohol ingestion. Our study was designed to investigate the association of cytochrome P450 2E1 (CYP2E1) genetic polymorphisms with the susceptibility to HCC in humans with and without chronic alcohol ingestion. We enrolled 171 male patients (108 Korean and 63 Japanese) with HCC and 31 age- and sex-matched healthy Korean subjects with no evidence of liver disease or cancer in any organ. Genotypes in the 5'-flanking region of the CYP2E1 gene were determined by restriction fragment length polymorphisms using 2 endonucleases: Pst I and Rsa I. Allelic frequencies in the CYP2E1 5'-flanking region in the Korean control population were 83.5% and 16.5% for allele c1 and c2, respectively. The frequencies of genotypes with the c2 allele (c1/c2 and c2/c2) were compared with those of genotypes without c2 (c1/c1) among HCC patients and controls, according to the pattern of alcohol consumption. There was no significant association between HCC risk and genotypes c1/c2 and c2/c2 either in all HCC patients or in HCC patients of different ethnic groups. Habitual drinkers with HCC, especially among Koreans, were more likely to carry genotype c1/c2 and c2/c2 (odds ratio=3.0) than non-habitual drinkers (odds ratio=1.2); however, the difference was not statistically significant. Even when patients were restricted to those without hepatitis B surface antigen and antibodies against hepatitis C virus but with a history of chronic alcohol ingestion, there was still no increased risk of HCC in those with genotypes c1/c2 and c2/c2. We conclude that there is a lack of association of the polymorphisms of CYP2E1 with the risk of HCC in humans. Int. J. Cancer 71: 737-740, 1997. © 1997 Wiley-Liss Inc.     

中国人肺癌易患性与CYP2E1基因多型性相关

目的　研究致癌物代谢酶细胞色素P45 0 2E1基因 (CYP2E1)多型性与肺癌风险的关系。方法　以PCR RFLP方法 ,分析 92例肺癌患者和 137例正常对照者RsaI识别的CYP2E1基因型。结果　c1/c1基因型频率在肺癌病例组为 72 8% ,显著 (P <0 0 1)高于对照组的 5 4 7%。多因素分析表明 ,携带c1/c1的个体发生肺癌的危险性比携带c1/c2和c2 /c2的个体高 2 5倍 (OR 2 5 ,95 %CI 1 8～ 3 8)。分层分析发现 ,c1/c1基因型主要增加肺鳞状细胞癌的危险性 (OR 2 6 ,95 %CI 2 3～ 5 8)。重要的是 ,研究发现CYP2E1c1/c1基因型与吸烟有协同作用。c1/c1基因型或吸烟单因素作用的OR分别为 3 9和 4 1,而二者联合作用的OR为 7 9;当吸烟量 <2 0包 年时 ,c1/c2和c2 /c2基因型的OR为 2 4,而c1/c1基因型的OR为 7 6 ;当吸烟量≥ 2 0包 年时 ,前者的OR为 5 5 ,而后者的OR增加到8 7。结论　CYP2E1c1/c1基因型是中国人肺癌的遗传易患性因素 ,此种基因型与吸烟有协同作用。