过氧化苯甲酰对雄性大鼠精液质量及睾酮水平的影响

目的研究过氧化苯甲酰对大鼠精液质量及睾酮水平的影响。方法将40只雄性大鼠按体重随机分为对照组,过氧化苯甲酰低、中、高剂量组。染毒16周后检测大鼠精子活动率、精子计数、精子畸形率和血清睾酮水平等指标。结果各剂量组精子活动率均低于对照组,中和高剂量组精子活动率与对照组比较,差异有统计学意义(P0.01)。低、中、高剂量组精子计数分别低于对照组,中、高剂量组精子计数明显低于对照组(P0.01)。各剂量组精子畸形率均高于对照组,中剂量组畸形率高于对照组,差异有统计学意义(P0.05),高剂量组畸形率更高(P0.01)。3个剂量组血清睾酮含量均低于对照组,差异有统计学意义(P0.01)。结论过氧化苯甲酰能降低雄性大鼠的生殖功能。     

农达染毒对30d龄雄性大鼠睾丸及附睾某些指标的影响

目的研究农达染毒对30 d龄雄性大鼠睾丸和附睾毒性、精子毒性及血清睾酮水平的影响,为农达的职业健康危险度评定提供科学依据。方法 30 d龄雄性SD大鼠40只,按体重随机分为4组,染毒低、中、高剂量组分别给250、500和1 000 mg/kg·bw的农达,连续灌胃30 d,对照组给予等量去离子水。观察各组大鼠一般情况,睾丸和附睾的脏器系数、病理学改变;精子总数、精子畸形率;用放免法测定各组大鼠血清睾酮含量。结果 1 000 mg/kg·bw染毒组大鼠睾丸和附睾系数明显下降(P0.01);染毒组大鼠生精小管上皮变薄,细胞层排列紊乱,生精细胞数目明显减少,附睾管腔中线状精子数减少;各染毒组大鼠精子总数均明显降低(P0.05),精子畸形率均明显升高(P0.05),精子畸形率与染毒剂量之间存在剂量-反应关系(P0.01);各染毒组大鼠血清睾酮含量均明显下降(P0.01)。结论 30 d龄雄性大鼠农达染毒可诱导其生殖毒性,表现为精子数量下降、畸形率增加和血清睾酮含量降低。     

亚慢性染毒纳米碳酸钙大鼠组织病理学研究

目的通过观察亚慢性染毒纳米碳酸钙大鼠器官组织病理学变化,初步探讨纳米碳酸钙的毒性和可能靶器官。方法选取健康SD大鼠100只,随机分为对照组、微米碳酸钙组(200 mg/kg)、纳米碳酸钙组(12.5、50和200 mg/kg)。滴鼻染毒,每周5次,连续12周。染毒结束24 h后处死大鼠,腹主动脉采血,全自动生化分析仪检测天冬氨酸转氨酶(AST)及丙氨酸转氨酶(ALT)活性和肌酐(Cr)、尿素氮(BUN)含量,取大鼠心、肝、脾、肺、肾、脑海马组织进行病理观察。结果对照组大鼠各器官均未见异常。微米碳酸钙组大鼠肺泡壁充血水肿,炎性细胞侵润。纳米碳酸钙组大鼠肺泡壁充血水肿,炎性细胞浸润,局部肺不张,部分小血管出现玻璃样变;支气管黏膜萎缩、剥脱,大量炎性细胞浸润。高剂量纳米碳酸钙组大鼠肾小球肿胀,肝细胞脂肪样变。纳米碳酸钙各组大鼠血清ALT活性和BUN含量显著高于对照组(P0.05),高剂量组大鼠血清BUN含量显著高于微米碳酸钙组(P0.05)。结论亚慢性染毒纳米碳酸钙可导致大鼠肺、肝、肾组织病理学损伤。     

土木香水煎液急性毒性和遗传毒性的研究

目的探讨土木香水煎液的急性毒性和遗传毒性,为土木香临床安全应用提供实验依据。方法采用最大耐受剂量法评价土木香水煎液的急性毒性,观察小鼠体重变化,心、肝、脾和肾的脏器指数变化;采用精子畸形实验,微核实验和彗星实验评价土木香水煎液的遗传毒性。将小鼠随机分为阴性对照组、阳性对照组和土木香水煎液高、中、低剂量组(分别灌胃蒸馏水,40 mg/kg·bw环磷酰胺,40、20、10 g/kg·bw土木香水煎液)。用药后取附睾制成精细胞悬液观察畸形精子数量。取骨髓细胞观察嗜多染红细胞的微核率。取肝、胸腺和脾细胞观察土木香水煎液对小鼠DNA的损伤。结果土木香水煎液经口给药小鼠最大耐受剂量为80 g/kg·bw,该剂量对小鼠的体重及各脏器指数无明显影响与阴性对照组比较差异无统计学意义(P0.05)。高和中剂量组的精子畸形率明显高于阴性对照组(P0.05),低剂量组精子畸形率与阴性对照组比较,差异无统计学意义(P0.05)。高剂量组的微核率明显高于阴性对照组,与之比较差异有统计学意义(P0.05),而中和低剂量组与阴性对照组比较,差异无统计学意义(P0.05)。各剂量组肝细胞、胸腺及脾细胞头尾DNA百分比、尾长、尾矩及Olive尾矩与阴性组比较差异无统计学意义(P0.05)。结论土木香的最大耐受计量为80 g/kg,属于无毒级。高和中剂量的土木香水煎液对生殖细胞有一定的毒性作用,且高剂量有潜在的遗传毒性。     

纳米二氧化硅粉尘对小鼠免疫功能的影响

目的研究纳米二氧化硅粉尘(nm-SiO2)对小鼠免疫功能的影响。方法 45只雄性昆明小鼠随机分3组(nm-SiO2粉尘高、低剂量组和对照组),除对照组外,纳米组的动物每天染毒1h,连续染毒14d。测定各组小鼠脾脏、胸腺系数和病理形态改变,淋巴细胞增殖功能、腹腔巨噬细胞吞噬功能和血清中的IgA、IgG和IgM的含量。结果①纳米各剂量组小鼠胸腺系数均高于对照组,差异无统计学意义(P0.05);纳米高剂量组小鼠脾脏系数高于对照组,差异有统计学意义(P0.05)。②T淋巴细胞增殖功能均下降,差异无统计学意义(P0.05)。③与对照组相比,纳米高、低剂量组小鼠腹腔巨噬细胞吞噬功能呈下降趋势,差异有统计学意义(P0.05)。④与对照组相比,纳米高、低剂量组小鼠血清中IgG的含量高于对照组(P0.05),IgA的含量低于对照组(P0.05),纳米高剂量组小鼠血清中IgM的含量低于对照组(P0.05),纳米低剂量组小鼠血清中IgM的含量高于对照组(P0.05);随着染毒剂量的增加,IgG的含量呈现增高趋势,IgA的含量呈现降低趋势(P0.05)。⑤nm-SiO2各染毒组小鼠胸腺皮质内淋巴细胞边界清楚,细胞结构完整。各剂量组显微镜下所见与对照组比较无改变。纳米染尘组脾脏肉眼可见体积增大,包膜紧张,显微镜下可见淋巴细胞明显增生,白髓所占比例增大。结论在本试验条件下,nm-SiO2粉尘可对小鼠免疫系统产生有害影响,并且这种有害作用存在剂量反应关系。     

丙烯腈对小鼠睾丸组织结构及精子质量的影响

目的探讨丙烯腈(acrylonitrile,ACN)对雄性小鼠睾丸组织的病理学损伤作用及超微结构的改变。方法将50只成年健康SPF级昆明种雄性小鼠,按体重随机分成阴性对照组(生理盐水0.01 ml/g),1.25、2.50和5.00 mg/kg ACN,连续腹腔注射5 d,1次/d;阳性对照组腹腔注射环磷酰胺(40 mg/kg)1次。于首日染毒后的第35天处死小鼠,取材,检测分析。结果 ACN各剂量组和阳性对照组精子密度和精子活率与对照组比较差异无统计学意义(P0.05),精子畸形率显著升高,且畸形精子构成都以无定型为主,无钩型次之。电子显微镜下可见睾丸支持细胞、间质细胞及精子细胞均有一定的变化,并且随着染毒剂量增加,其病变程度加重。结论 ACN能够诱导小鼠睾丸损伤,具有性腺毒性。     

纳米氧化镍致大鼠肺毒性实验研究

目的探讨纳米氧化镍急性染毒致大鼠急性肺毒性的研究。方法将50只Wistar大鼠,雌雄各半,按体质量(180~220 g)分为5组,分别为0.9%氯化钠注射液对照组、微米氧化镍对照组(20 mg/ml)及纳米氧化镍(0.8、4、20 mg/ml)组。以非暴露式气管滴注法染毒,3 d 1次,染尘15 d后,腹主动脉抽血处死。分别对肺泡灌洗液和血清中总蛋白、白蛋白、碱性磷酸酶、酸性磷酸酶、乳酸脱氢酶的含量进行检测。结果大鼠支气管肺泡灌洗液中总蛋白含量、白蛋白含量、乳酸脱氢酶活力、碱性磷酸酶活力和酸性磷酸酶活力与0.9%氯化钠注射液对照组相比,纳米氧化镍中、高剂量组均升高且差异有统计学意义(P<0.05),各剂量组血清中总蛋白、白蛋白、碱性磷酸酶、酸性磷酸酶、乳酸脱氢酶水平的变化与对照组比较差异无统计学意义(P>0.05),纳米染毒剂量组之间的差异无统计学意义(P>0.05),同等剂量纳米与微米染毒组间的差异也无统计学意义(P>0.05)。结论纳米氧化镍可能对大鼠肺组织有急性毒性,且有剂量效应关系。     

纳米二氧化硅对SD大鼠精子和睾丸组织的影响

目的探讨不同剂量的纳米二氧化硅(nm-SiO2)对SD雄性大鼠精子和睾丸组织的影响。方法采用鼻腔滴注法,将SD大鼠连续暴露于不同剂量nm-SiO_2(浓度分别为10、20和30mg/kg·bw)4周后,在显微镜下观察精子数量、活动度和畸形率,用石蜡病理切片苏木素-伊红(HE)染色法观察大鼠睾丸组织的病理改变,用高效液相色谱串联质谱分析nm-SiO_2对SD大鼠睾丸组织基因组DNA甲基化水平的影响。结果与对照组相比,20和30mg/kg·bw纳米组的精子数量和精子活力均降低(P0.05),精子畸形率增加(P0.05)。暴露于各个剂量组的睾丸组织曲精细管发生不同程度的病理改变。相较于对照组,各个剂量组nm-SiO_2均使睾丸组织基因组DNA甲基化水平降低,且呈现剂量-效应关系。结论nm-SiO_2暴露可导致SD大鼠睾丸组织形态学改变,对精子造成一定的损伤,并且随着nm-SiO_2剂量增加,其对大鼠精子发育损伤逐渐增大。nm-SiO_2暴露可以导致SD大鼠睾丸组织基因组DNA甲基化水平下降。     

土木香水煎液急性毒性和遗传毒性的研究CSCD

目的探讨土木香水煎液的急性毒性和遗传毒性,为土木香临床安全应用提供实验依据。方法采用最大耐受剂量法评价土木香水煎液的急性毒性,观察小鼠体重变化,心、肝、脾和肾的脏器指数变化;采用精子畸形实验,微核实验和彗星实验评价土木香水煎液的遗传毒性。将小鼠随机分为阴性对照组、阳性对照组和土木香水煎液高、中、低剂量组(分别灌胃蒸馏水,40 mg/kg·bw环磷酰胺,40、20、10 g/kg·bw土木香水煎液)。用药后取附睾制成精细胞悬液观察畸形精子数量。取骨髓细胞观察嗜多染红细胞的微核率。取肝、胸腺和脾细胞观察土木香水煎液对小鼠DNA的损伤。结果土木香水煎液经口给药小鼠最大耐受剂量为80 g/kg·bw,该剂量对小鼠的体重及各脏器指数无明显影响与阴性对照组比较差异无统计学意义(P>0.05)。高和中剂量组的精子畸形率明显高于阴性对照组(P<0.05),低剂量组精子畸形率与阴性对照组比较,差异无统计学意义(P>0.05)。高剂量组的微核率明显高于阴性对照组,与之比较差异有统计学意义(P<0.05),而中和低剂量组与阴性对照组比较,差异无统计学意义(P>0.05)。各剂量组肝细胞、胸腺及脾细胞头尾DNA百分比、尾长、尾矩及Olive尾矩与阴性组比较差异无统计学意义(P>0.05)。结论土木香的最大耐受计量为80 g/kg,属于无毒级。高和中剂量的土木香水煎液对生殖细胞有一定的毒性作用,且高剂量有潜在的遗传毒性。     

双酚A对小鼠睾丸组织氧化损伤及生殖细胞凋亡的影响

目的研究双酚A(bisphenolA,BPA)对雄性小鼠睾丸组织氧化损伤及其对生殖细胞凋亡的影响。方法将52只健康成年雄性昆明种小鼠随机分为溶剂对照组(玉米油)和低(75 mg/kg·bw)、中(150 mg/kg·bw)、高(300 mg/kg·bw)剂量组,每组13只,连续经口染毒8周。处死小鼠后,解剖取材,称量睾丸的重量并计算脏器系数;取附睾,制成精子混悬液于镜下观察精子活动率、统计精子畸形率;采用酶标仪检测睾丸组织匀浆中超氧化歧化酶(SOD)、乳酸脱氢酶(LDH)活力及丙二醛(MDA)含量;采用qPCR方法检测凋亡相关基因CytC、Caspase-3及Bcl-2 mRNA表达量。结果各染毒剂量组较对照组睾丸脏器系数升高,精子活力随剂量升高明显降低(P0.05);精子畸形数及精子畸形率均随剂量升高明显升高(P0.05);各染毒剂量组睾丸组织SOD、LDH酶活力及MDA含量均升高,差异有统计学意义(P0.05);各染毒剂量组睾丸组织中CytC及Caspase-3 mRNA表达量较对照组均升高,Bcl-2 mRNA表达量较对照组降低,差异有统计学意义(P0.05)。结论 BPA引起雄性小鼠睾丸组织的氧化损伤及生殖细胞凋亡。     

Induction of DNA strand breaks in peripheral lymphocytes by soluble chromium compounds.

1. Incubation of human lymphocytes with sodium dichromate (CrVI) at 37 degrees C for 3 h resulted in a dose-dependent increase in DNA strand breaks without concurrent cytotoxicity. In contrast, chromium acetate hydroxide (CrIII) failed to induce DNA strand breaks at sub-cytotoxic concentrations. 2. DNA strand breaks were also detected in the peripheral lymphocytes of Wistar rats, 24 h after intratracheal instillation of sodium dichromate (1.3 and 2.5 mg kg-1). Instillation of chromium acetate hydroxide (up to 21.8 mg kg-1) failed to induce DNA strand breaks in peripheral lymphocytes. In accord with previous studies, hexavalent chromium was found to be more readily absorbed from the lungs into the peripheral blood than chromium in its trivalent form. 3. The results of this study indicate that fluorometric analysis of DNA unwinding (FADU) in peripheral lymphocytes might be a convenient method of measuring an important biological effect of chromium in occupationally-exposed workers.     

合成鱼腥草素对脾切除动物细胞免疫功能的影响

目的　研究合成鱼腥草素对脾切除动物细胞免疫功能的影响及作用机制。方法　建立小鼠、大鼠脾切除模型 ,测定迟发型超敏反应强度、外周血淋巴细胞ANAE阳性百分率、胸腺T细胞增殖能力、淋巴结T细胞增殖能力、淋巴结中淋巴细胞数量、外周血T细胞亚群。结果　合成鱼腥草素ig (6 0mg·kg-1,12 0mg·kg-1)能明显增强脾切除小鼠迟发型超敏反应强度 ,ConA诱导的淋巴结T淋巴细胞增殖能力 ,增加外周血淋巴细胞ANAE阳性百分率、淋巴结中淋巴细胞数量 ,而对胸腺T淋巴细胞增殖能力则无明显影响 ;合成鱼腥草素ig (4 0mg·kg-1,80mg·kg-1)明显升高脾切除大鼠Th 数量 ,降低Ts 数量 ,调节外周血T淋巴细胞亚群Th/Ts 比值。结论　合成鱼腥草素对脾切除动物细胞免疫功能具有调节作用 ,其作用主要是通过增强脾切后淋巴结的功能及调节T细胞亚群来实现的     

外周血泌乳素水平与T淋巴细胞亚群的相关性研究

目的本通过研究外周血PRL水平与T淋巴细胞亚群变化的相关性,以探讨泌乳素（PRL）与免疫系统变化的关系。方法检测100例垂体腺瘤患者及56例体检健康人外周血中CD4＋CD25＋、CD3＋、CD3＋CD4＋及CD3＋CD8＋的T淋巴细胞水平;并测定其外周血中PRL水平。结果病例组外周血CD3＋T淋巴细胞显著低于健康人组（P〈0.0001）;外周血CD3＋CD4＋T淋巴细胞及CD3＋CD8＋T淋巴细胞病例组与健康人组均无显著差异（P〉0.05）;病例组外周血CD4＋CD25＋T淋巴细胞为显著高于健康人组（P〈0.0001）。其中病例组中PRL〈100ng/ml组外周血CD3＋T淋巴细胞及CD3＋CD4＋T淋巴细胞均显著低于PRL〉100ng/ml组（P〈0.05）;外周血CD3＋CD8＋T淋巴细胞及CD4＋CD25＋T淋巴细胞,病例组与健康人组均无显著差异（P〉0.05）。结论 CD3＋T淋巴细胞的减少可能是垂体肿瘤细胞本身刺激CD4＋CD25＋T淋巴细胞的增生,导致外周血IL-2减少,从而抑制了T淋巴细胞的活化和增殖。外周血PRL〉100ng/ml组外周血PRL显著升高,通过刺激各种细胞因子的分泌,而进一步促进T淋巴细胞的活化及增殖,同时,外周血中高PRL可直接抑制外周血T淋巴细胞的凋亡。     

Effect of Lycium barbarum polysaccharide on the improvement of antioxidant ability and DNA damage in NIDDM rats

The effects of polysaccharide extracted from Lycium barbarum (LBP) on blood glucose, oxidative stress and DNA damage in rats with non-insulin dependent diabetes mellitus (NIDDM) were studied. The results show that LBP treatment (10 mg/kg.d) for 4 weeks led to decreased levels of blood glucose, malondialdehyde (MDA) and nitric oxide (NO) in serum of fasting rats; and to increased serum level of superoxidedismutase (SOD). Furthermore, LBP could reduce cellular DNA damage in peripheral lymphocytes of NIDDM rats. The DNA damage was determined by using the single cell gel (comet) assay with alkaline electrophoresis and was quantified by measuring tail length and tail moment. These results suggest that LBP can control blood glucose and modulate the metabolism of glucose, leading to significant improvement of oxidative stress markers (SOD, MDA) in rats with NIDDM. And that, LBP decreases DNA damage possibly via a decrease in oxidative stress levels. In conclusion, LBP as a dietary supplement may prevent the development of complications or even tendency to carcinogenesis in NIDDM rats.     

Evaluation of cytogenetic and DNA damage induced by the antibacterial drug, trimethoprim.

Trimethoprim, a widely used antimicrobial drug was tested for its effect on the level of nuclear DNA damage in cultured peripheral blood lymphocytes in terms of chromosome and DNA alterations. The extent of cytogenetic damage, expressed as chromosome breakage and chromosome loss, was evaluated employing the cytokinesis block micronucleus method (CBMN) in cultured peripheral blood lymphocytes coupled with fluorescence in situ hybridization (FISH) using a digoxigenin-labelled alphoid DNA probe specific for the centromere of all human chromosomes. The DNA breakage level was evaluated by the Comet assay. Cultures were set up by using blood samples from two healthy donors. A range of concentrations of the test agent (from 1 to 100 microg/ml) was used for the micronuclei (MN) frequency and cytogenetic origin of MN. For the Comet assay the range of doses used was from 0.5 to 150 microg/ml. From the results obtained it appears that this antifolic agent has a significant clastogenic potential, as detected by a dose-dependent increase of the incidence of C-MN and significantly greater than control levels at the highest concentrations tested (25,100 microg/ml). In addition, the results obtained in the Comet assay also show that trimethoprim induces a dose-dependent increase in the level of DNA breakage, this increase attaining statistical significance at the highest concentrations tested (25, 100, 150 microg/ml), which would confirm its genotoxicity.     

Effects of some mycotoxins on mitogen-induced blastogenesis and SCE frequency in human lymphocytes

The effects of T-2 toxin, diacetoxyscirpenol, ochratoxin A and zearalenone on DNA synthesis in phytohaemagglutinin-stimulated human peripheral blood lymphocytes were studied by assaying the incorporation of [3H]thymidine. Total inhibition was obtained with 8 ng T-2 toxin/ml, 8 ng diacetoxyscirpenol/ml or 30 micrograms zearalenone/ml, and with 20 micrograms ochratoxin A/ml inhibition was almost complete; 50% inhibition was produced by 1.5 ng T-2 toxin/ml, 2.7 ng diacetoxyscirpenol/ml, 14 micrograms zearalenone/ml or 14 micrograms ochratoxin A/ml. The toxicity of the trichothecenes to the lymphocytes was slightly reduced when rat liver cells were present whereas the toxicity of ochratoxin A and zearalenone was unaltered. Low concentrations of trichothecenes did not produce any inhibition of DNA synthesis when the cultures were washed and placed in fresh media containing only phytohaemagglutinin 4 hr after the addition of the test compounds. Sister chromatid exchange frequency was not elevated by T-2 toxin, diacetoxyscirpenol or ochratoxin A. Zearalenone had a weak enhancing effect on the frequency of sister chromatid exchanges.     

Dopaminergic drug-induced modulation of the expression of the dopamine transporter in peripheral blood lymphocytes in Parkinson's disease

The modulation of expression of the dopamine transporter by dopaminergic drugs was investigated by flow cytometry in peripheral blood lymphocytes from patients suffering Parkinson's disease. An 8-week in vivo exposure to pramipexole (0.7 mg free base, 3 times a day) or ropinirole (12 mg, once daily), but not levodopa/carbidopa (100/25 mg, 3 times a day), significantly reduced the mean fluorescence intensity of the dopamine transporter in peripheral blood lymphocytes. These results demonstrate that levodopa differs from dopamine agonists in its regulation of dopamine transporter expression in peripheral blood lymphocytes.     

高渗盐溶液对失血性休克大鼠细胞免疫及p38MAPK信号通路的影响

目的 探讨7.5%氯化钠高渗盐溶液(HSS)对失血性休克大鼠脾脏T淋巴细胞功能的影响以及p38MAPK信号转导途径是否参与该过程.方法 SD雄性大鼠遭受失血性休克(平均动脉压40 mmHg)60 min后分别用生理盐水、HSS以及HSS SB203580(SB)10 mg/kg(ip)复苏.免疫荧光观察脾T淋巴细胞中p38蛋白的表达水平,同时ELISA法检测脾T淋巴细胞IL-2含量,MTT法测定脾T淋巴细胞增殖率.结果 免疫荧光显示HSS组T淋巴细胞p38MAPK表达显著高于NS组和HSS SB组,HSS组脾淋巴细胞增值率显著高于NS组和HSS SB组(P＜0.01),HSS组IL-2 含量显著高于NS组和HS SB组(P＜0.01).结论 HSS可能通过p38MAPK信号转导途径改善失血性休克机体的脾脏T淋巴细胞功能.     

Activity of N-acetyl-beta-glucosaminidase in lymphocytes of rats exposed to mixture of nitrogen oxides and chlorine

Activity of N-acetyl-beta-glucosaminidase (GS) has been cytochemically studied in peripheral blood lymphocytes of rats exposed to the mixture of nitrogen oxides (1.22 mg/m³) and chlorine (1.02 mg/m³) during a period of 12 weeks. The decrease of the total lymphocyte count and an increase of the count of GS-positive lymphocytes with cytoplasmatic but not exclusively lysosomal localization have been noted after exposure. The increase of the count of lymphocytes containing the enzyme within both lysosomal granules and cytoplasm has been correlated with the exposure time and accompanied by diminishing the count of lymphocytes containing the enzyme within the lysosomal granules exclusively. The authors believe the changes noted above may result from toxic damage of lysosomes or the immune response of lymphocytes against antigens released from the damaged tissues.     

Enzymatic changes in peripheral blood leukocytes in rats in subacute benzene vapours poisoning

The activity of acid phosphatase (AcP), its intracellular distribution in peripheral blood lymphocytes and the activity of lysosomal isoenzyme of acid phosphatase (AcPL) in serum of rats exposed to benzene vapour in the concentration 27 000 mg/m3 for 6 hours daily through 10 consecutive days were investigated. The statistically significant fall in the number of lymphocytes affecting AcP-positive and AcP-negative lymphocytes, reduction in the percentage of granular lymphocytes and increase in the percentage of granular-diffusive and diffusive lymphocytes were found. The increase in the activity of AcPL in serum was statistically insignificant. According to the authors opinion the phenomenon observed results from a damage to lymphocytic lysosomes by benzene.