短时受精失败后补行ICSI的实验室指标和新生儿情况分析

目的：探讨常规体外受精(IVF)失败后补行卵胞浆内单精子显微注射(ICSI)的实验室指标和新生儿情况。方法：回顾性分析2011年1－12月在成都锦江妇幼保健院接受ICSI治疗的患者203例(对照组)和IVF失败后补行ICSI的患者117例(实验组)的实验室检查结果、临床数据和新生儿情况。结果：实验组患者多精受精率(3.00%)显著高于对照组(1.39%)(P<0.05)。对照组中有1例引产,1例早产儿在产后10d夭折。实验组与对照组的正常受精率、卵裂率、D3优胚率、囊胚形成率、冷冻周期率、移植周期率、胚胎着床率、妊娠率、多胎妊娠率、异位妊娠率、早期流产率、晚期流产率、活胎分娩率以及胎儿畸形率差异均无统计学意义(P＞0.05)。新生儿情况对比发现,两组间婴儿性别比、身长、体质量、Apgar评分差异亦无统计学意义(P＞0.05)。单、双胎妊娠新生儿中,实验组孕周、体质量、身长和Apgar评分与对照组比较差异均无统计学意义。结论：常规IVF受精失败后,及时补行ICSI能显著提高受精率和优胚率,进而改善临床结局。     

人精子穿去透明带金黄地鼠卵实验和精子DNA完整性检测在体外受精-胚胎移植中的应用

目的：探讨人精子穿去透明带金黄地鼠卵实验（SPA）和精子DNA完整性检测在体外受精-胚胎移植术（IVF-ET）助孕中的应用价值。方法：选择兰州大学第一医院生殖医学研究中心,行IVF-ET助孕的不孕不育夫妇中的41名男性,根据不孕不育原因分为完全男方因素引起的不孕不育组（n=20）和完全女方因素引起的不孕不育组（n=21）,比较分析两组男方年龄、穿透率、受精指数、IVF受精率、DNA碎片率、卵裂率、优质胚胎率、胚胎着床率及临床妊娠率的差别。结果：完全男方因素引起的不孕不育组和完全女方因素引起的不孕不育组之间,在男方年龄、IVF受精率、卵裂率、胚胎着床率、临床妊娠率等方面的差异均无统计学意义（P均〉0.05）,在穿透率、受精指数、DNA碎片率方面的差异均具有统计学意义（P〈0.05）;前者的SPA穿透率与IVF受精率、卵裂率和优质胚胎率之间均存在显著正相关（r=0.908,0.55,0.852,P均〈0.05）,DNA碎片率与IVF受精率和优质胚胎率均存在显著负相关（r=-0.636,-0.634,P均〈0.05）;后者SPA穿透率与IVF受精率、卵裂率和优质胚胎率之间均存在显著正相关（r=0.893,0.53,0.626,P均〈0.05）,DNA碎片率与IVF受精率和优质胚胎率之间均存在显著负相关（r=-0.636,-0.634,P均〈0.05）。结论：SPA和精子DNA完整性检测在辅助生殖技术中可能具有一定的应用价值。     

复脑苏对孕鼠致畸性的研究

背量与目的:评价复脑苏在妊娠期的毒性. 材料与方法:用82只SD大鼠孕鼠,分为3个不同剂量的复脑苏(7.5 ms/kg、15.0 mg/kg、30.0 mg/kg)实验组,同时设阴性对照组(0.85%NS),每组19～21只孕鼠,实验组和对照组给药容量为10 ml/kg体重.实验组和阴性对照组均采用尾静脉给药,给药时间为妊娠期第6 d～15 d.于妊娠第20 d处死孕鼠,检查妊娠母体与胎鼠畸形情况.结果:复脑苏在各剂量组的活胎率、死胎率和吸收胎率及致畸率与对照组相比较差异无统计学意义(P＞0.05);各实验组胎鼠体重、身长、尾长与对照组比较差异也无统计学意义(P＞0.05);实验组的平均胎盘重与对照组相比较差异均具有统计学意义(P＜0.05),但无明显的剂量-效应关系.各实验组除尾椎数和远端趾骨数与对照组相比差异具有统计学意义外(P＜0.05),其它骨骼发育与对照组比较差异均无统计学意义(P＞0.05).各实验组均未观察到母鼠和胎鼠明显的外观、脏器以及骨骼的畸形.结论:复脑苏在本实验剂量(≤30.0 mg/kg)下无明显的母鼠毒性和致畸作用,也无明显的胚胎毒性和胎鼠毒性.     

囊肿核摘除术联合促性腺激素释放激素激动剂对卵巢囊肿患者卵巢储备功能及妊娠结果的影响

目的探讨囊肿核摘除术联合促性腺激素释放激素激动剂(GnHR-a)对卵巢囊肿患者卵巢储备功能及妊娠结果的影响。方法选取2013年7月至2015年7月间临朐县人民医院收治的120例卵巢囊肿患者,按照是否接受手术联合治疗分为观察组和对照组,每组60例。再将两组患者按照手术类型分为双侧囊肿核摘除术组与单侧囊肿核摘除术组,每组各30例。对比各组窦卵泡数、GnRHa天数、促性腺激素(Gn)支数、人绒毛膜促性腺激素(HCG)注射日E_2、卵泡数、卵子数、好胚数、冷冻数、移植数、妊娠率、流产率、分娩率和着床率。结果双侧囊肿核摘除对照组与双侧囊肿摘除观察组相比,窦卵泡数减少、Gn支数增加、HCG注射日E_2水平降低、卵泡数、卵子数、好胚数、冷冻数、移植数均减少,差异均有统计学意义(均P<0.05)。双侧囊肿核摘除对照组与双侧囊肿摘除观察组妊娠率、流产率和分娩率比较,差异无统计学意义(P>0.05)。单侧囊肿核摘除联合GnHR-a治疗观察组与单侧囊肿核摘除对照组比较,窦卵泡数减少、Cn支数增加、卵泡数减少、卵子数减少,差异有统计学意义(P<0.05)。单侧囊肿核摘除联合GnHR-a治疗观察组与单侧囊肿摘除对照组比较,HCG注射日E_2水平、好胚数、冷冻数、移植数、妊娠率、流产率、分娩率的差异均无统计学意义(均P>0.05)。结论单侧或双侧囊肿核摘除术均可对患者的卵巢储备功能造成影响,联合GnHR-a治疗可明显改善生殖功能下降情况,且不影响生育能力,应引起临床工作者的重视。     

妊娠合并子宫肌瘤同期行剖宫产术和子宫肌瘤剔除术的疗效观察

目的探讨妊娠合并子宫肌瘤患者同期行剖宫产术和子宫肌瘤剔除术的效果。方法选取2017年11月至2019年11月间北京市平谷区医院收治的68例妊娠合并子宫肌瘤患者,根据治疗方法不同进行分组,采用剖宫术治疗的34例患者纳入对照组,采用剖宫产术联合子宫肌瘤剔除术治疗的34例患者纳入研究组。比较两种治疗方法实际应用效果的差异性。结果相较于对照组,研究组手术时间更长,差异有统计学意义(P <0. 05);两组患者疼痛评分、术中出血量、住院时间及肛门排气时间比较,差异无统计学意义(P>0. 05)。两组患者月经恢复时间、产后出血量、恶露持续时间及产后并发症比较,差异无统计学意义(P> 0. 05)。两组新生儿身长、5min Apgar评分、体质量及新生儿窒息比较,差异无统计学意义(P>0. 05)。结论在妊娠合并子宫肌瘤临床治疗中,可同期实施剖宫产术和子宫肌瘤剔除术,手术安全性较高,母婴健康状况较好。     

妊娠合并子宫肌瘤对妊娠及分娩结局的影响

目的探讨妊娠合并子宫肌瘤对妊娠及分娩结局的影响。方法选取2012年1月至2015年1月间收治的妊娠合并子宫肌瘤患者110例作为观察组,同时选择同期分娩无子宫肌瘤的112例产妇作为对照组,观察比较两组患者妊娠晚期并发症、产时和产后并发症、分娩方式及术中处理情况。结果观察组孕期合并先兆流产、早产和胎位异常的发生率分别为11.8%、13.6%和19.1%,均显著高于对照组的5.4%、3.6%和8.0%,差异均有统计学意义(P<0.05);观察组妊娠时胎盘早剥的发生率为6.4%,显著高于对照组的0.9%,差异有统计学意义(P<0.05);两组患者产后出血发生率差异无统计学意义(P>0.05);观察组新生儿宫内窘迫和新生儿窒息发生率分别为8.2%和13.6%,显著高于对照组的1.8%和4.5%,差异有统计学意义(P<0.05),且观察组患者新生儿Apgar评分显著低于对照组,差异有统计学意义(P<0.05);观察组产妇剖宫产率为51.8%,显著高于对照组的42.9%,差异有统计学意义(P<0.05)。结论妊娠合并子宫肌瘤对产妇及新生儿均有一定的不良影响,产科医师应根据不同孕期、肌瘤大小,在确保孕产妇及胎儿安全的前提下,施行个体化治疗。     

宫颈环状电切术对早期宫颈癌患者生育能力和妊娠率的影响

目的探讨宫颈环状电切术(LEEP)对早期宫颈癌患者生育能力和妊娠率的影响。方法选取2013年6月至2015年6月间临朐县人民医院收治的40例早期宫颈实施宫颈锥切术患者为观察组,另选取同期门诊收治的40例无宫颈锥切术史计划的妇女为对照组。随访2年,对比两组产妇的妊娠情况、分娩方式、围生儿结局。结果与对照组比较,观察组患者的妊娠率和分娩率降低,不孕率提高,差异均有统计学意义(均P<0.05)。观察组患者中,输卵管性不孕5例(12.5%),宫颈粘连不孕1例(2.5%),宫颈管狭窄不孕1例(2.5%)。对照组中输卵管性不孕2例(5.0%)。与对照组比较,观察组患者的平产率降低,剖宫产率升高,差异均有统计学意义(均P<0.05)。与对照组比较,观察组患者的胎膜早破发生率、早产率、新生儿窒息率和新生儿低体重率明显升高,差异均有统计学意义(均P<0.05)。结论采用LEEP刀锥切术对早期宫颈癌患者的生育能力可造成一定的影响,一方面降低了妊娠率,另一方面提高了胎膜早破、早产、新生儿窒息和新生儿低体重发生率,应引起临床医师的重视。     

阴式子宫肌瘤剔除术对患者术后妊娠分娩结局的影响

目的探讨阴式子宫肌瘤剔除术对患者术后妊娠分娩结局的影响。方法选取2012年5月至2013年5月间收治的67例行阴式子宫肌瘤剔除术后妊娠产妇为观察组,另选取同期分娩的70例正常孕妇为对照组。对两组的妊娠分娩结局(早产、剖宫产、产后出血情况以及新生儿窒息和病死率)进行比较。结果观察组患者的早产率、剖宫产率、产后出血率均高于对照组,其中早产率和产后出血率间差异无统计学意义,(均P>0.05),剖宫产率间差异有统计学意义(P<0.05)。观察组患者新生儿窒息率和病死率均高于对照组,但差异均无统计学意义(P>0.05)。结论阴式子宫肌瘤剔除术不会对患者的术后妊娠分娩结局产生明显影响,是一种安全可靠的治疗方式。     

年轻宫颈癌患者行腹腔镜下卵巢移位及阴道延长治疗的疗效分析

目的 探讨年轻宫颈癌患者腹腔镜下行卵巢移位及阴道延长治疗的临床优势.方法 按照手术方式不同将96例宫颈癌患者分为实验组(50例)和对照组(46例),实验组患者腹腔镜下行广泛全子宫切除术+盆腔淋巴结清扫术+卵巢异位+阴道延长术,对照组患者行腹式广泛全子宫切除术+盆腔淋巴结清扫术+卵巢异位+阴道延长术,比较两组治疗效果.结果 实验组患者术中出血量明显少于对照组,差异具有显著性(P＜0.05);两组患者手术时间和淋巴结切除个数比较,差异无统计学意义(P＞0.05);实验组患者肛门排气时间和术后住院时间均明显短于对照组,差异具有显著性(P＜0.05);实验组患者术后并发症发生率明显低于对照组,差异具有显著性(P＜0.05);两组患者手术前后FSH、LH、E2水平、阴道长度与性功能评分比较,差异无统计学意义(P＞0.05);组间比较,差异也无统计学意义(P＞0.05).结论 年轻宫颈癌患者行腹腔镜下卵巢移位及阴道延长治疗创伤小、术后恢复快、并发症少,对患者性功能和内分泌水平影响小,临床应用价值较高.     

不同类型黏膜下子宫肌瘤患者宫腔镜手术治疗对妊娠结局的影响

目的探讨不同类型黏膜下子宫肌瘤患者行宫腔镜手术治疗对妊娠结局的影响。方法选取2011年3月至2014年6月间深圳市龙岗区第六人民医院收治的162例黏膜下子宫肌瘤患者,均采用宫腔镜电切除术治疗。根据子宫肌瘤与肌层的关系,将患者分为0型组(78例)、Ⅰ型组(54例)、Ⅱ型组(30例),对3组患者的妊娠结局进行统计对比。结果 0型组患者的足月生产率、流产率、早产率和难产率与Ⅰ型和Ⅱ型组比较,差异均有统计学意义(均P<0.05)。Ⅰ型组与Ⅱ型组患者的足月生产率、流产率和早产率比较,差异无统计学意义(P>0.05)。但Ⅰ型组患者的难产率显著低于Ⅱ型组,差异有统计学意义(P<0.05)。3组患者均未发生子宫破裂(P>0.05)。结论不同类型黏膜下子宫肌瘤患者实施宫腔镜电切除术治疗的妊娠结局存在差异,带蒂肌瘤、肌层扩展越浅,患者的妊娠结局越好,提示黏膜下子宫肌瘤应当早诊断、早治疗。     

The Outcome of IVF/ICSI Cycles in Male Cancer Patients: Retrospective Analysis of Procedures from 2004 To 2018

IntroductionFertility preservation is an important aspect of quality of life in oncological patients, and in men is achieved by semen cryopreservation prior to treatment. Results of in vitro fertilization (IVF) procedures in healthy infertile couples are comparable, regardless of whether fresh or cryopreserved semen is used, but are scarce in male oncological patients.Patients and methodsWe performed a retrospective analysis of IVF/intracytoplasmic sperm injection (IVF/ICSI) procedures in infertile couples where men had been treated for cancer in the past. We additionally compared the results of IVF/ICSI procedures with respect to the type of semen used (fresh, cryopreserved).ResultsWe compared the success rates of 214 IVF/ICSI cycles performed in the years 2004–2018. Pregnancy (30.0% vs. 21.4%; p = 0.12) and live-birth rates (22.3% vs. 17.9%; p = 0.43) per oocyte aspiration were similar between the groups in fresh cycles; however embryo utilization (48.9% vs. 40.0%; p = 0.006) and embryo cryopreservation rates (17.3% vs. 12.7%; p = 0.048) were significantly higher in the cryopreserved semen group. The cumulative pregnancy rate (60.6% vs. 37.7%; p = 0.012) was significantly higher, and the live-birth rate (45.1% vs. 34.0%; p = 0.21) non-significantly higher, in the cryopreserved semen group.ConclusionsThe success of IVF/ICSI procedures in couples where the male partner was treated for cancer in the past are the same in terms of pregnancies and live-births in fresh cycles regardless of the type of semen used. However, embryo utilization and embryo cryopreservation rates are significantly higher when cryopreserved semen is used, leading to a significantly higher cumulative number of couples who achieved at least one pregnancy.Key words: assisted reproduction techniques, infertility, fertility preservation, sperm cryopreservation, pregnancy     

Twinning and the Incidence of Breast and Gynecological Cancers (United States)

Increasing epidemiological and experimental evidence indicates that the carcinogenic pathway in the breast and female reproductive organs is driven, at least in part, by factors associated with reproduction. We conducted a retrospective cohort study, comparing the risk of ovarian, breast, endometrial, and cervical cancers among women who had records of at least one twin pregnancy, compared with women who had given birth to only single children. Subjects were selected from the Utah Population Database, which consists of multiple linked datasets including genealogy, births and deaths and cancer registries. We used Poisson regression to calculate relative risks, adjusted for the number of pregnancies and the age of the mother at the birth of first and last children, with singleton mothers as the reference group in each case. The risks of breast and ovarian cancers did not differ between mothers of twins and mothers of single children. The risk of endometrial cancer was slightly lower in mothers of twins than in mothers of singleton children (RR = 0.90, 95% CI 0.67-1.21). Conversely the risk of cervical cancer was higher among twin mothers (RR = 1.78, 95% CI 0.88-3.52). This latter finding supports previous data suggesting that reproductive hormones act as cofactors in the etiology of cervical cancer.     

The risk of preterm birth and growth restriction in pregnancy after cancer

It is unclear whether cancer and its treatments increase the risk of adverse pregnancy outcomes. Our aim was to examine whether cancer survivors have higher risks of poor outcomes in pregnancies conceived after diagnosis than women without cancer, and whether these risks differ by cancer type and race. Diagnoses from cancer registries were linked to pregnancy outcomes from birth certificates in three U.S. states. Analyses were limited to the first, live singleton birth conceived after diagnosis. Births to women without a previous cancer diagnosis in the registry were matched to cancer survivors on age at delivery, parity, race/ethnicity and education. Log‐binomial regression was used to estimate risk ratios. Cervical cancer survivors had higher risks of preterm birth (Risk ratio = 2.8, 95% Confidence interval: 2.1, 3.7), as did survivors of invasive breast cancer (RR = 1.3, 95% CI: 1.1, 1.7) and leukemia (RR = 2.1, 95% CI: 1.3, 3.5). We observed a higher risk of small for gestational age (SGA) infants (<10% of weight for age based on a national distribution) in survivors of brain cancer (RR = 1.7, 95% CI: 1.1, 2.8) and extranodal non‐Hodgkin lymphoma (RR = 2.3, 95% CI: 1.5, 3.6). We did not see an increased risk of infants born preterm, low birth weight, or SGA in pregnancies conceived after ductal carcinoma in situ, thyroid cancer, melanoma, or Hodgkin lymphoma. While our results are reassuring for survivors of many cancers, some will need closer monitoring during pregnancy.     

The relationship between twin births and maternal risk of breast cancer: a meta-analysis

Women who undergo a greater number of menstrual cycles may be at increased risk of breast cancer, possibly due to cumulative exposure to ovarian hormones. Pregnancy reduces the lifetime number of menstrual cycles and also influences the levels of ovarian hormones. Twin pregnancies differ from singleton pregnancies in both hormone levels and perinatal changes. To date, a meta-analysis on the effects of twin birth on the risk of maternal breast cancer has not been conducted. Among 17 relevant publications identified in a systematic search, some suggest that twin births may be associated with lower breast cancer risk but others do not; therefore, the results are inconclusive. Although our pooled results of all 17 published studies did not show a reduced maternal risk of breast cancer for twin births (HR 0.94; 95% CI = 0.87-1.02; P = 0.127), a trend toward reduced maternal risk of breast cancer was identified in a subgroup analysis of cohort studies (HR 0.91; 95% CI = 0.83-1.01; P = 0.068). The results of this meta-analysis suggest that twin pregnancy does not significantly decrease the maternal risk of breast cancer.     

麦绿素的致畸和致突变作用

目的： 评价麦绿素有无致畸和致突变作用。方法：采用小鼠急性经口毒性试验、小鼠微核试验和大鼠致畸试验检测麦绿素有无致畸和致突变作用。急性经口毒性试验中选用最大耐受剂量法 (MTD),剂量为15 g/kg;微核试验中设麦绿素1.25、2.5和5.0 g/kg剂量组、阴性和阳性对照组,通过两次 (间隔24 h)给小鼠灌胃给予受试物后,取其股骨骨髓涂片,染色观察其嗜多染红细胞中微核发生率;致畸试验中设麦绿素1.25、2.5、5.0 g/kg组、阴性和阳性对照组,于Wistar大鼠受孕第7天给受试物,连续10 d,于受孕第20天处死孕鼠,观察胎鼠的外观,生长发育情况,固定后检测胎鼠骨骼和内脏畸形种类,计算畸形率。结果：急性毒性试验中麦绿素对雌雄小鼠经口MTD均大于15 g/kg,属无毒级;小鼠骨髓细胞微核试验中各剂量组微核率与阴性对照组比较,差异均无统计学意义(P>0.05),而阳性对照组微核率与阴性对照组及各剂量组比较差异均有统计学意义(P均<0.01);大鼠致畸试验中,与阴性对照组比较,各剂量组孕鼠与胎鼠的生长发育差异均无统计学意义(P>0.05),胎鼠的畸形率亦无统计学意义(P>0.05), 而阳性对照组胎鼠内脏、骨骼畸形率与阴性对照组及各剂量组比较差异均有统计学意义(P均<0.01)。结论：麦绿素对大鼠无致畸作用,对小鼠无致突变作用。     

The role of prophylactic cerclage in preventing preterm delivery after electrosurgical conization

Objective

To evaluate pregnancy outcomes after electrosurgical conization.

Methods

We retrospectively analyzed the outcomes of 56 singleton pregnancies after electrosurgical conization of the uterine cervix. Of the 56 cases, 25 women underwent prophylactic cerclage with McDonald procedure (cerclage group), and 31 were managed expectantly (expectant group). Pregnancy outcomes including rate of preterm delivery were compared, and the effect of potential risk factors such as depth of cone, interval between conization and pregnancy, and cervical length on the risk of preterm delivery was assessed.

Results

The rate of preterm delivery was significantly higher in women with a history of electrosurgical conization than those without (32.1% vs. 15.2%, p<0.001). However, preterm delivery rate was not different between the two groups (expectant group vs. cerclage group; <28 week, 6.5% vs. 8.0%, p=1.000; <34 week, 19.4% vs. 20.0%, p=1.000; <37 week, 29.0% vs. 36.0%, p=0.579). All obstetric and neonatal outcomes were similar in the two groups. Even when we confined the study subjects to 19 women (19/56, 33.9%) with cervical length less than 25 mm, the preterm delivery rate also was not significantly different between the expectant (n=7) and cerclage group (n=12). Finally, the potential risk factors for preterm delivery were not associated with risk of preterm delivery in patients with a history of electrosurgical conization.

Conclusion

The rate of preterm delivery was significantly higher in women with a history of electrosurgical conization before pregnancy. However, prophylactic cervical cerclage did not prevent preterm delivery in these patients.     

妊娠合并卵巢肿瘤的临床诊断和妊娠结局分析

目的:探讨妊娠合并卵巢肿瘤的临床诊断和妊娠结局,总结预防感染措施。方法:随机选取2011年2月-2013年4月75例妊娠合并卵巢肿瘤患者为研究对象,作为观察组,同时期的75例妊娠合并瘤样病变患者为对照组。从临床表现、辅助检查上比较妊娠合并卵巢肿瘤患者临床特征,并分析其妊娠结局,观察采取预防感染措施后感染发生情况。结果:妊娠合并卵巢肿瘤通常无明显的临床症状,有症状则以恶心呕吐、腹痛等为主。和瘤样病变比较,其肿瘤直径增大明显,肿瘤血流阻力指数下降,孕期肿块均较瘤样病变明显。观察组CA125增高46例,平均值为（31.53±14.73）U/ml,对照组CA125增高41例,平均值为（29.27±12.23）U/ml,两组比较无明显差异性（P>0.05）。两组患者CA199、CEA、AFP检测结果比较差异无统计学意义,均在正常范围内。妊娠合并卵巢肿瘤的妊娠结局以足月产为主,占全部的74.67%,早产和流产占全部的25.33%。在不同时间段有不同的妊娠结局,＜12周以流产为主,12-28周以早产和流产较多,>28周-足月则以足月产为主。感染发生率为4%。结论:妊娠合并卵巢肿瘤通常无临床症状,超声和实验室检查有助于鉴别诊断,早期干预对妊娠结局至关重要。     

应用显性致死试验检测西番莲果汁的遗传毒性

目的： 应用小鼠显性致死试验评价西番莲果汁的遗传毒性。方法：西番莲果汁7.2～28.8 g/kg剂量对NIH种雄性小鼠连续灌胃染毒5 d,再与雌性小鼠连续同笼交配7周,每批雌性小鼠在妊娠第13～14天处死,观察胚胎着床数、活胎数和死胎数。同时设置对照组。对所有计数、计量资料进行统计学处理。结果：环磷酰胺(CP)和丝裂霉素C(MMC)阳性对照小鼠除平均着床数正常外,其余各项指标在不同周次中均存在异常(P<0.05或<0.01)。阴性对照组和西番莲果汁各剂量组的小鼠交配率>133%,妊娠率在60%～93%,窝平均着床数>8.6只,平均死胎数<0.8只,平均活胎数>8.1只,均在正常数值范围内;各剂量西番莲果汁均未诱发有死胎孕鼠率、显性致死率和突变指数的增高及着床卵生存率的降低(P>0.05)。结论：西番莲果汁在本试验剂量(7.2～28.8 g/kg)范围内,小鼠显性致死试验为阴性结果,提示其无遗传毒性。     

阿利苯多对大鼠的围产期毒性

目的： 观察阿利苯多对大鼠妊娠晚期、分娩期、哺乳期及胚胎和胎仔出生后生长发育、学习能力以及生殖能力的影响。方法：从大鼠妊娠第15 天至哺乳第21天,分别连续灌胃给予50、100和200 mg/ (kg·d)剂量的阿利苯多,同时设0.5％ 羧甲基纤维素钠为溶剂对照组,观察各组大鼠和胎仔的生长、发育、生殖能力等指标。结果：F0代未见明显毒性反应。F1代,在出生第1天、第4天及雄性性成熟时,低剂量组仔鼠体质量与溶剂对照组比较显著增加 (P＜0.05);低剂量组张耳达标时间及中剂量组的痛阈时间与溶剂对照组比较差异显著 (P＜0.05),但均无量效关系;其余各指标及学习和运动能力与溶剂对照组比较差异无统计学意义(P＞0.05),另F1代的同笼交配率、妊娠率及胚胎发育全过程未见明显的生殖、发育等毒性变化。结论：在本实验条件下,阿利苯多对妊娠/哺乳的雌性动物以及胚胎和子代发育、行为及生殖无明显毒性。     

卵巢子宫内膜异位囊肿剥离术后囊肿复发与否对接受IVF-ET治疗疗效的影响

目的:探讨卵巢子宫内膜异位囊肿剥离术后囊肿复发与否对IVF-ET治疗疗效的影响。方法:选取2016年01月至2018年12月在我院妇产科生殖中心就诊,有过一次卵巢子宫内膜异位囊肿剥离术史、准备接受IVF-ET治疗的92例患者,根据术后囊肿是否复发分为复发组41例,未复发组51例。分别比较两组患者体质量指数、卵泡刺激素、促黄体素、雌二醇、窦卵泡计数、Gn启动量、Gn总使用量、hCG注射日内膜厚度、成熟卵泡个数、E2水平、获卵数、MII卵数、卵裂数、优质胚胎率、临床妊娠率、胚胎着床率、早期流产率、活产率、周期取消率等指标。结果:复发组患者hCG注射日成熟卵泡个数明显少于未复发组（P＜0.05）,两组之间Gn启动量、Gn总使用量、hCG日内膜厚度、hCG日E2水平以及IVF率、ICSI率均无明显差异（P＞0.05）。复发组患者获卵数、MII卵数、卵裂数、优质胚胎率均明显低于未复发组,而周期取消率明显高于未复发组（P＜0.05）,两组患者胚胎着床率、临床妊娠率、早期流产率、活产率无明显差异（P＞0.05）。结论:卵巢子宫内膜异位囊肿剥离术后囊肿复发对于不孕患者有不良影响,但是经IVF-ET治疗后,依然可以得到与非复发组患者相似的临床活产率,临床需个体化选择治疗方案。