手足口病并发脑干脑炎患儿血清NSE、NPY和TNF-α检测的临床意义

目的：探讨了手足口病并发脑干脑炎患儿血清NSE、NPY和TNF-α水平的变化及临床意义。方法：应用放射免疫分析对34例手足口病并发脑干脑炎患儿进行血清NSE、NPY和TNF-α检测,并与30名正常健康儿进行比较。结果：手足口病患儿血清NSE、NPY和TNF-α水平非常显著地高于正常儿组（P〈0.01）,且TNF-α与NSE、NPY水平呈正相关（r=0.4716、0.5184,P〈0.01）。结论：检测手足口病并发脑干脑炎患儿血清NSE、NPY和TNF-α水平的变化,对判断病情和评价疗效均有重要的临床价值。     

缺血缺氧性脑病患儿血清NSE和血浆NPY检测的临床意义

目的：探讨了缺血缺氧性脑病患儿血清NSE和血浆NPY水平的变化及意义。方法：应用放射免疫分析对30例缺血缺氧性脑病患儿进行了血清NSE和血浆NPY测定,并与30名正常新生儿作比较。结果：缺血缺氧性脑病患儿血清NSE和血浆NPY水平均非常显著地高于正常新生儿组（P〈0.01）,且两者呈明显的正相关（r=0.6021,P〈0.01）。结论：测定缺血缺氧性脑病患儿血清NSE和血浆NPY水平的变化与疾病的发生发展和预后密切相关。     

ACI患者治疗前后血浆ET-1和血清NSE、NPY联检的临床意义

目的:评估了急性脑梗死(ACI)患者治疗前后血浆内皮素-1(ET-1)和血清神经元特异性烯醇化酶(NSE)、神经肽-Y(NPY)水平的变化及临床意义.方法:应用放射免疫分析对32例ACI患者进行了治疗前后血浆ET-1和血清NSE、NPY检测,并与35名正常健康人作比较.结果:在治疗前血浆ET-1和血清NSE、NPY水平...     

急性脑梗死患者治疗前后血清NSE、血浆NPY水平检测的临床意义

目的：探讨了急性脑梗死患者血清神经元特异性烯醇化酶（NSE）和血浆神经肽Y（NPY）水平的变化及临床意义。方法：应用放射免疫分析对38例急性脑梗死患者进行了血清NSE和血浆NPY水平测定,并以35名正常健康人作比较。结果：急性脑梗死患者血清NSE和血浆NPY水平非常显著地高于正常人组（P〈0．01）,经治疗3个月后血清NSE和血浆NPY仍有差异（P〈0．05）。结论：急性脑梗死患者血清NSE和血浆NPY水平的变化与急性脑梗死的发病机理有密切的关系。     

手足口病并发脑干脑炎患儿血清NPY、Hcy和S100β测定的临床意义

目的：探讨手足口病并发脑干脑炎患儿NPY、Hcy和S100β水平的变化及临床意义。方法：应用放射免疫分析、免疫化学法和酶联法对32例手足口病并发脑干脑炎患儿进行了血清NPY、Hcy和S100β检测,并与35例正常健康儿进行比较。结果：手足口病患儿血清NPY、Hcy和S100β水平均非常显著地高于正常儿组（P〈0.01）,且血清NPY水平与Hcy和S100β水平呈正相关（r=0.4812、0.5984,P〈0.01）。结论：检测手足口病并发脑干脑炎患儿NPY、Hcy和S100β水平,对判断病情和评价疗效均有重要的临床价值。     

慢性肾炎患儿治疗前后血清IL-18、IL-10、TNF-α和血浆VEGF水平的变化

目的:探讨了慢性肾炎患儿治疗前后血清白细胞介素-18(IL-18)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)和血浆血管内皮生长因子(VEGF)水平的变化及意义.方法:分别采用放射免疫分析和ELISA检测了30例慢性肾炎患儿血清IL-18、IL-10、TNF-α和血浆VEGF含量,并与35例正常健康儿作比较.结果:慢性肾炎患儿在治疗前血清IL-18、IL-10、TNF-α和血浆VEGF含量均显著高于正常儿组(P＜0.01),经中西医结合治疗3个月后与正常儿比较,仍有显著差异(P＜0.05).结论:检测慢性肾炎患儿血清IL-18、IL-10、TNF-α和血浆VEGF水平的变化对观察病情和预后判定具有重要的临床价值.     

AD患者血浆ET和血清NSE、NPY检测的临床意义

目的：探讨了阿尔茨海默病（AD）患者血浆ET和血清NSE、NPY水平的变化及意义。方法：应用放射免疫分析对31例AD患者进行了血浆ET和血清NSE、NPY测定,并与30名正常健康人作比较。结果：AD患者血浆ET和血清NSE、NPY水平均非常显著地高于正常人组（P〈0.01）,且ET水平与NSE、NPY水平呈正相关（r=0.4895、0.6014,P〈0.01）。结论：检测AD患者血浆ET和血清NSE、NPY水平的变化对判断病情和评价疗效均具有重要的临床价值。     

新生儿缺血、缺氧性脑病患儿血浆INS、NPY检测的临床意义

目的:探讨了新生儿缺血、缺氧性脑病患儿血浆胰岛素(INS)、神经肽Y(NPY)水平的变化及意义.方法:应用放射免疫分析测定了94例新生儿缺血、缺氧性脑病患儿血浆INS和NPY水平及相关性研究,并与30名正常新生儿作比较.结果:新生儿缺血、缺氧性脑病患儿血浆INS和NPY水平均非常显著地高于正常新生儿组(P＜0.01),且两者呈明显正相关(r=0.6112,P＜0.01).结论:测定新生儿缺血、缺氧性脑病患儿血浆INS和NPY水平的变化与疾病的危重程度、发生发展和预后密切相关.     

支气管哮喘患儿治疗前后血清GM-CSF、CGRP检测的临床意义

目的:探讨了支气管哮喘患儿血清巨噬细胞集落刺激因子(GM-CSF)和降钙素基因相关肽(CGRP)水平的变化及意义.方法:应用放射免疫分析对33例支气管哮喘患儿进行了血清GM-CSF和CGRP测定,并与35名正常健康儿作比较.结果:支气管哮喘患儿在治疗前血清GM-CSF水平非常显著地高于正常儿组(P＜0.01),血清CGRP水平则显著地低于正常儿组(P＜0.01),经治疗3个月后则与正常儿组比较无显著性差异(P＞0.05).结论:血清GM-CSF水平的升高和CGRP水平的降低是支气管哮喘患儿发病的病理因素之一,有重要临床价值.     

急性脑梗死患者血浆NPY、CGRP及血清IGF-Ⅰ水平测定

目的:探讨脑梗死患者血浆NPY、CGRP及血清IGF-Ⅰ水平的变化及其临床意义.方法:随机选择了32例脑梗死患者,采用放射免疫分析分别测定了患者治疗前后的血浆NPY、CGRP及血清IGF-Ⅰ水平,并将组间的差异进行了统计学处理及分析.结果:32例脑梗死患者治疗前NPY水平较对照组升高极显著(P＜0.01),CGRP及IGF-Ⅰ水平则较对照组下降均非常显著(P均＜0.01);经治疗患者血浆NPY水平较治疗前已显著降低(P＜0.05),血浆CGRp及血清IGF-Ⅰ水平较治疗前则均明显上升(P均＜0.05).结论:脑梗死患者血浆NPY、CGRP及血清IGF-Ⅰ水平的变化与其发病关系密切,其测定对于了解患者的病情程度及制订治疗方案有重要临床价值.     

Studies on the structure and function of the apolipoprotein(a) gene

Lp(a) is an LDL-like lipoprotein that is a major inherited risk factor for atherosclerosis. It is distinguished from Lp(a) by the addition of apolipoprotein(a). The gene structure of apolipoprotein(a) is homologous to plasminogen, and competition with plasminogen activity may account for some of the pathophysiology associated with Lp(a). Six highly related genes have now been identified, and at least four are found in close proximity in overlapping genomic clones. Studies have begun on the regulation of apolipoprotein (a) gene expression, and the human apolipoprotein(a) gene has been inserted into transgenic mice, where it leads to the development of arterial lesions.     

Vacuum Ultraviolet Photolysis of Hydrocarbon Polymers

Summary: The wavelength‐dependent vacuum ultraviolet (VUV) photolysis of several polymers, low density polyethylene (LDPE), biaxially oriented poly(propylene) (BOPP), atactic polystyrene (PS), and poly(methyl methacrylate) (PMMA), was studied by irradiation in vacuum with the well‐characterized emissions from four different resonant or excimer VUV sources. These lamps comprise radiofrequency (r.f.) discharges in different noble gases, such as krypton, xenon (at low pressures, producing near‐monochromatic resonant line radiations), xenon excimer (at “high” pressure), and a deuterium/argon mixture (producing a broad‐band emission). VUV‐induced mass loss (ablation or etching) was monitored in situ by quartz crystal microbalance measurements. Following irradiation, samples were analysed by ATR‐FTIR and XPS, to evaluate near‐surface structural changes (e.g., creation of unsaturation, cross‐linking) resulting from the VUV‐initiated bond scissions and radical‐creation reactions. PMMA was the most readily ablatable polymer, whereas the mass loss of BOPP was higher than that of LDPE, regardless of the irradiation wavelength, λ. All polymers were found to form double bonds, with the exception of PS, which is rather stable, probably due to energy dissipation by fluorescence.

Formation of double bonds in a) vinyl‐, b) vinylidene‐, and c) vinylene‐like unsaturated groups, as a function of the radiation dose, D, for KrL (?), XeL (?), and D₂Ar‐irradiated (?) PMMA.     

新生儿缺氧缺血性脑病患者血浆ET、SS测定的临床意义

目的:探讨了新生儿缺氧缺血性脑病患者血浆内皮素(ET)和生长抑素(SS)水平的变化及临床意义.方法:应用放射免疫分析对63例新生儿缺氧缺血性脑病患者进行了血浆ET和SS检测,并与35名正常健康新生儿作比较.结果:病儿组血浆ET水平显著高于正常儿组,而SS水平则显著低于正常儿组(P＜0.01).结论:新生儿缺氧缺血性脑病的发生发展与ET和SS水平的变化密切相关.     

Distribution of six neuropeptides in the nucleus tractus solitarii of the rat: an immunohistochemical analysis

Distribution of substance P-, [Leu]enkephalin-, cholecystokinin-8-, neurotensin-, avian pancreatic polypeptide- and gamma-melanocyte stimulating hormone-like immunoreactive structures were investigated in the nucleus tractus solitarii of the rat by means of the indirect immunofluorescence method. The density of the immunoreactive structures varied markedly according to neuropeptides or subnuclei, with the medial and commissural nuclei containing the highest density. This suggests that the peptides examined play a role in cardiovascular function. However, as seen in the substance P- and [Leu]enkephalin-like immunoreactive structures, these peptides were widely distributed in the nucleus tractus solitarii in addition to the commissural and medial nuclei; a high density of immunoreactive fibers in the ventral, dorsolateral and intermediate subnuclei. In addition to the immunoreactive fiber plexus, a group of immunoreactive cells was also identified in the subnuclei mentioned above. These findings strongly suggest that substance P- and [Leu]enkephalin-like immunoreactive structures are involved not only in cardiovascular function but also in other functions such as respiration, at least in the rat. Finally, the present study demonstrated that the area postrema, particularly its lateral portion, contains various neuropeptide-like structures, both neurons and fibers, substance P-, [Leu]enkephalin-, cholecystokinin-8- and neurotensin-like immunoreactive neurons and fibers, and avian pancreatic polypeptide- and gamma-melanocyte stimulating hormone-like immunoreactive fibers.     

脑血栓形成患者LPA、MDA、SOD检测的临床分析

目的:探讨溶血磷脂酸(LPA)和氧自由基在脑血栓形成的病理过程中的作用.方法:通过检测90例脑血栓形成患者(CT组)血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、LPA的浓度情况,并与短暂性脑缺血发作(TIA)组(45例)、对照组(45例)比较.结果:血清中SOD的浓度,CT组(治疗前)低于TIA组和对照组(P<0...     

聚羟基烷酸酯、聚丁二酸丁二酯及其共混材料的细胞相容性评价比较

取家兔的骨髓间充质干细胞(MSCs)分别种植到聚羟基烷酸酯(PHBV)、聚丁二酸丁二酯(PBS)、及其二者共混材料(PHBV/PBS)上,每组材料又分为有孔和实心两种构型。用MTT实验、乳酸脱氢酶(LDH)实验、甲苯胺蓝染色和电镜等方法,观察MSCs在材料上的生长情况。比较三种材料六种构型分别对MSCs黏附和增殖的影响。结果以共混物实心材料的毒性最低,细胞增殖率最高。甲苯胺蓝染色结果显示兔MSCs在共混实心材料上的生长最佳,扫描电镜结果显示兔MSCs与共混实心材料黏附良好。共混物实心材料很有希望成为制备组织工程血管新型的支架材料。     

The regulation of non-coding RNA expression in the liver of mice fed DDC

Mallory-Denk bodies (MDBs) are found in the liver of patients with alcoholic and chronic nonalcoholic liver disease, and hepatocellular carcinoma (HCC). Diethyl 1,4-dihydro-2,4,6,-trimethyl-3,5-pyridinedicarboxylate (DDC) is used as a model to induce the formation of MDBs in mouse liver. Previous studies in this laboratory showed that DDC induced epigenetic modifications in DNA and histones. The combination of these modifications changes the phenotype of the MDB forming hepatocytes, as indicated by the marker FAT10. These epigenetic modifications are partially prevented by adding to the diet S-adenosylmethionine (SAMe) or betaine, both methyl donors. The expression of three imprinted ncRNA genes was found to change in MDB forming hepatocytes, which is the subject of this report. NcRNA expression was quantitated by real-time PCR and RNA FISH in liver sections. Microarray analysis showed that the expression of three ncRNAs was regulated by DDC: up regulation of H19, antisense Igf2r (AIR), and down regulation of GTL2 (also called MEG3). S-adenosylmethionine (SAMe) feeding prevented these changes. Betaine, another methyl group donor, prevented only H19 and AIR up regulation induced by DDC, on microarrays. The results of the SAMe and betaine groups were confirmed by real-time PCR, except for AIR expression. After 1 month of drug withdrawal, the expression of the three ncRNAs tended toward control levels of expression. Liver tumors that developed also showed up regulation of H19 and AIR. The RNA FISH approach showed that the MDB forming cells' phenotype changed the level of expression of AIR, H19 and GTL2, compared to the surrounding cells. Furthermore, over expression of H19 and AIR was demonstrated in tumors formed in mice withdrawn for 9 months. The dysregulation of ncRNA in MDB forming liver cells has been observed for the first time in drug-primed mice associated with liver preneoplastic foci and tumors.     

血清C肽浓度测定的临床意义探讨

目的： 研究血清C肽浓度及胰岛素与C肽比值的临床意义。方法：用放射免疫分析测定2型糖尿病和肾功能不全患者血清C肽、胰岛素浓度,并计算胰岛素与C肽比值。结果：肾功能不全组病人血清C肽、胰岛素水平显著高于糖尿病各组,且胰岛素与C肽比值显著低于糖尿病各组（P〈0．01）;在糖尿病各年龄组中,胰岛素水平无明显变化（P〉0．05）;但血清C肽水平随年龄增大而升高,而胰岛素与C肽比值则显著下降（P〈0．01）。结论：测定血清C肽水平及胰岛素与C肽比值有助于了解糖尿病患者的肾功能状况,值得进一步深入探讨。     

妊高征患者血浆ET-1和血清INH、D-D检测的临床意义

目的:探讨了血浆内皮素-1(ET-1)和血清抑制素(INH)、D-二聚体(D-D)水平在妊高征患者发病中的变化和临床意义.方法:采用放射免疫分析、酶联法和生化法对32例妊高征患者进行了血浆ET-1和血清INH、D-D检测,并与35名正常孕妇作比较.结果:妊高征患者血浆ET-1和血清INH、D-D水平均非常显著地高于正常...     

仿生型BG-COL-HYA-PS复合支架细胞相容性

目的:研究仿生型BG-COL-PS-HYA复合支架材料与成骨细胞的相容性。方法:将小鼠胚胎成骨细胞种植于BG-COL-HYA-PS、BG-COL复合材料、58SBG支架上,用MTT法、ALP活性测定等观察细胞在材料中的生长情况。通过体外实验方法,观察其生物相容性。结果:成骨细胞在BG-COL-HYA-PS材料上能良好粘附、增殖,而在58SBG材料上粘附差、细胞逐渐死亡。MTT法结果显示:联合培养后,BG-COL-HYA-PS组的OD值为0.314±0.004,5天时达到0.621±0.002,分别为58SBG组的1.49倍和1.44倍,P<0.05。结论:仿生型BG-COL-PS-HYA复合支架具有天然骨分级结构和有良好的生物相容性,在诱导成骨细胞增殖方面性能优越,可作为骨组织工程支架材料,临床应用前景广阔。