脑梗塞与红细胞胆固醇、血液超氧化物歧化酶、过氧化脂质水平关系探讨

检测１０９例脑梗塞患者和１００例健康对照者红细胞胆固醇（Ｅ－Ｃｈ）含量、血浆和红细胞超氧化物歧化酶（Ｐ－ＳＯＤ、Ｅ－ＳＯＤ）活性及血浆和红细胞过氧化脂质（Ｐ－ＬＰＯ、Ｅ－ＬＰＯ）含量的结果表明，患者组Ｅ－Ｃｈ、ＰＬＰＯ、Ｅ－ＬＰＯ平均含量皆显著高于对照组（Ｐ＜０．０５～０．００１），Ｐ－ＳＯＤ、Ｅ－ＳＯＤ平均活性皆显著低于对照组（Ｐ＜０．００１）；患者病情随Ｅ－Ｃｈ含量升高而加重，呈直线相关；患者Ｅ－Ｃｈ含量随Ｐ－ＳＯＤ、Ｅ－ＳＯＤ活性下降而升高，随Ｐ－ＬＰＯ、Ｅ－ＬＰＯ含量上升而升高，均呈直线相关。提示脑梗塞患者红细胞膜内脂质代谢异常与体内氧自由基反应和脂质过氧化反应病理性加剧有一定程度的相关性。     

脑梗塞与红细胞胆固醇,血液超氧化物歧化酶,过氧化脂质水平…

检测１０９例脑梗塞患者和１００例对照者红细胞胆固醇（Ｅ－Ｃｈ）含量、务 和红细胞超氧化物歧化酶（Ｐ－ＳＯＤ、Ｅ－ＳＯＤ）活性及血浆和红细胞过氧化脂质（Ｐ－ＰＯ、Ｅ－ＬＰＯ）含量的结果表明，患者组Ｅ－Ｃｈ、Ｐ－ＬＰＯ、Ｅ－ＬＰＯ平均含量皆显著高于对照组（Ｐ〈０．０５－０．００１），Ｐ－ＳＯＤ、Ｅ－ＳＯＤ平均活性皆显著低于对照组（Ｐ〈０．００１）；患者病情随Ｅ－Ｃｈ含量升高而加重，呈直线相关；患者Ｅ－     

多发性脑梗塞痴呆患者部分抗氧化指标检测及临床意义

检测配对设计的５４例多发性脑梗塞痴呆患者的５４例健康老年人血浆维生素Ｃ、血浆维生素Ｅ含量及血浆和红细胞超氧化物歧化酶活性、血浆和红细胞过氧化脂质、Ｅ－ＳＯＤ值均显著降低（Ｐ＜０．００１），平均Ｐ－ＬＰＯ和Ｅ－ＬＰＯ含量均显著升高；５４例患者的Ｐ－ＶＣ、Ｐ－ＶＥ含量均与Ｐ－ＳＯＤ、Ｅ－ＳＯＤ值呈直线正相关，均与Ｐ－ＬＰＯ、Ｅ－ＬＰＯ含量呈直线负相关；患者病程与Ｐ０ＶＣ、Ｐ－ＶＥ、Ｅ－ＳＯＤ值呈直线负     

脑中风与红细胞胆固醇,过氧化脂质和超氧化物歧化酶水平关系的研究

检测１０９例脑梗塞、１０５例脑出血患者和１００例健康对照者红细胞胆固醇（Ｅ－Ｃｈ）、血浆和红细胞过氧化脂质（Ｐ－ＬＰＯ、Ｅ－ＬＰＯ）含量及超氧休物歧化酶（Ｐ－ＳＯＤ、Ｅ－ＳＯＤ）活性的结果表明，与对照组比较，脑梗塞组Ｅ－Ｃｈ显著升高，脑出血组Ｅ－Ｃｈ显著降低；２患者组Ｐ－ＬＰＯ、Ｅ－ＬＰＯ显著升高，Ｐ－ＳＯＤ、Ｅ－ＳＯＤ显著降低；Ｅ－Ｃｈ与脑梗塞患者病情及Ｐ－ＬＰＯ、Ｅ－ＬＰＯ、Ｐ－ＳＯＤ、Ｅ－Ｓ     

吸烟者血浆VitC、VitE、β－CAR、LPO和E－SOD值的检测

检测３８４例健康老年吸烟者和１７６例健康老年非吸烟者血浆维生素Ｃ（Ｐ－ＶｉｔＣ）、维生素Ｅ（Ｐ－ＶｉｔＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）、过氧化脂质（Ｐ－ＬＰＯ）含量和红细胞超氧化物歧化酶（Ｅ－ＳＯＤ）活性的结果表明，与非吸烟组比较，吸烟组的Ｐ－ＶｉｔＣ、Ｐ－ＶｉｔＥ、Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ平均值显著降低（Ｐ＜０．００１），而Ｐ－ＬＰＯ平均值显著升高（Ｐ＜０．００１）；８２例６１岁男性吸烟者Ｐ－ＶｉｔＣ、Ｐ－ＶｉｔＥ、Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ均随吸烟史及吸烟量的增加而降低，Ｐ－ＬＰＯ随吸烟史及吸烟量的增加而升高，并均呈一定程度的直线相关（Ｐ＜０．０００１）。提示老年吸烟者体内的氧化和抗氧化平衡严重失调，氧自由基反应和脂质过氧化反应明显加剧。     

脑梗塞患者红细胞变形能力与红细胞膜微粘度,膜脂质及血浆脂质…

作者检测脑梗塞患者及健康对照组的ＲＢＣ变形能力、ＲＢＣ膜微粘度、膜脂质及血浆脂质后发现，脑梗塞患者ＲＢＣ变形性显著低于对照组，其变形能力与ＲＢＣ膜微粘度、膜胆固醇（Ｃｈ）／磷脂（ＰＬ）比值呈显著负相关，与血浆ＡｐｏＡ－Ⅰ呈显著正相关；ＲＢＣ膜胆固醇与血浆ＡｐｏＡ－Ⅰ及ＨＤＬ－Ｃｈ呈显著负相关，与ＡｐｏＢ呈显著正相关，但与血浆Ｃｈ无相关关系。     

健康成人增龄过程中血脂血糖变化的观察

对127例无代谢内分泌异常及其他相关疾病的健康成人空腹血糖、血清总胆固醇、甘油三脂及β脂蛋白测值的多元逐步回归分析表明，增龄为空腹血糖及血脂的独立影响因素，其间均呈正相关关系。同时，血糖尚与性别呈负相关，与收缩压以及甘油三脂呈正相关；而甘油三脂与性别呈负相关。故健康成人在增龄过程中，血脂血糖有随龄俱增的倾向。     

脑梗塞患者红细胞变形能力与红细胞膜微粘度、膜脂质及血浆脂质的关系

作者检测脑梗塞患者及健康对照组的ＲＢＣ变形能力、ＲＢＣ膜微粘度、膜脂质及血浆脂质后发现，脑梗塞患者ＲＢＣ变形性显著低于对照组，其变形能力与ＲＢＣ膜微粘度、膜胆固醇（Ｃｈ）／磷脂（ＰＬ）比值呈显著负相关，与血浆ＡｐｏＡ－Ｉ呈显著正相关；ＲＢＣ膜胆固醇与血浆ＡｐｏＡＩ及ＨＤＬ－Ｃｈ呈显著负相关，与ＡｐｏＢ呈显著正相关，但与血浆Ｃｈ无相关关系。     

老年人吸烟与血浆一氧化氮、维生素C和E、β-胡萝卜素浓度关系的探讨

目的探讨老年人吸烟与血浆一氧化氮（Ｐ－ＮＯ）、维生素Ｃ（Ｐ－ＶＣ）和Ｅ（Ｐ－ＶＥ）、β－胡萝卜素（Ｐ－β－ＣＡＲ）浓度的关系。方法检测３８４例健康老年吸烟者和１７６例健康老年非吸烟者的Ｐ－ＮＯ、Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ浓度，在Ｃｏｍｐａｑ５８６计算机上用ＳＰＳＳ／ＰＣ＋统计软件包进行对比分析。结果与非吸烟组比较，吸烟组的Ｐ－ＮＯ平均浓度显著升高及Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ平均浓度皆显著降低（均为Ｐ＜０．００１）；８２例６１岁男性健康吸烟者随吸烟史和日吸烟量的增加，其Ｐ－ＮＯ浓度升高，Ｐ－ＶＣ、Ｐ－ＶＥ、Ｐ－β－ＣＡＲ浓度均降低，均呈一定程度的直线相关（Ｐ＜０．０００１）。结论吸烟是导致老年人体内ＮＯ代谢异常及氧化和抗氧化平衡严重失调的重要原因之一。     

吸烟者血浆VitC,VitE,β—CAR,LPO和E—SOD值的检测

检测３８４例健康老年吸烟者和１７６例健康老年非吸烟者血浆维生素Ｃ，维生素Ｅ，β－胡萝卜素，过氧化脂质含量和红细胞超氧化物歧化酶活性的结果表明，与非吸烟组比较，吸烟组的Ｐ－ＶｉｔＣ，Ｐ－ＶｉｔＥ，Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ平均值显著降低，而Ｐ－ＬＰＯ平均值显著升高，８２例６１岁男性吸烟者Ｐ－ＶｉｔＣ，Ｐ－ＶｉｔＥ，Ｐ－β－ＣＡＲ和Ｅ－ＳＯＤ随吸烟史及吸烟量的增加而降低，Ｐ－ＬＰＯ随吸烟吸及吸烟量的增     

Determinant of postprandial triglyceride levels in healthy young adults

BackgroundFasting lipid profile does not necessarily illustrate the exact lipid dynamic in 24 h as human spends most of their time in postprandial state. Postprandial triglyceride (TG) has been reported to have advantages compared to fasting TG in terms of practicality and ability to predict cardiovascular events. This study aims to assess the determinant of postprandial TG in healthy young adults.MethodsThis is a quasy-experimental study that involved 200 healthy young adults. This study compared fasting with postprandial TG and analyzed the relationship between postprandial TG with various demographic and metabolic parameters after ingestion of standardized high fat liquid meal.ResultThere was an upward trend from fasting TG to 2 h postprandial TG and 4 h postprandial TG. There was strong correlation between fasting TG and 2 h postprandial TG with 4 h postprandial TG (r = 0.731; p < 0.0001 dan r = 0.669; p < 0.0001, respectively). Whereas body mass index (BMI) and age showed weak correlation with 4 h postprandial TG (r = 0.141; p < 0.0001 dan r = 0.0747; p < 0.0001), fasting TG was the strongest predictor of 4 h postprandial TG (r = 0.669, B = 1.722 (95% CI 1.552 to 1.892), p < 0.0001).ConclusionFasting TG was the strongest determinant of 4 h postprandial TG in healthy young adults. We also observed strong correlation between 4 h postprandial TG and fasting TG. Hence, 4 h postprandial TG might potentially replaced fasting TG when measurement of fasting TG is not feasible.     

Association of plasma B-type natriuretic peptide levels with shunt size in young adults with atrial septal defect

Objective: This study was designed to investigate the validity of brain natriuretic peptide (BNP) levels for the estimation of the shunt size in young adults with atrial septal defect (ASD), and to determine the relationship between BNP levels and echocardiographic parameters of right heart chambers. Methods: Fifty‐six patients with ASD (mean age 22.9 ± 2.0 years) were studied. The control group consisted of 31 age‐gender matched healthy volunteers (mean age 22.7 ± 1.9 years). Coventional echocardiography, tissue Doppler imaging (TDI) and plasma BNP level measurement was performed in all participants. The ratio of pulmonary to systemic blood flow (Qp/Qs) was measured noninvasively using transthoracic echocardiography. Results: Plasma BNP levels were significantly higher in ASD patients than in controls (42.9 ± 29.4 vs. 8.3 ± 2.6 pg/mL, P < 0.05). Pulmonary artery pressure (PAP) (P = 0.0001), right atrium (RA) volume (P = 0.0001), and right ventricular end‐diastolic volume (RVEDV) (P = 0.0001) values were higher in ASD patients. There was a powerful correlation between plasma BNP levels and Qp/Qs ratio (r = 0.71, P < 0.0001). The plasma BNP levels significantly correlated with PAP (r = 0.61, P < 0.0001), RA volume (r = 0.54, P < 0.0001), RVEDV (r = 0.55, P < 0.0001), and right ventricular myocardial performance index (r = 0.50, P < 0.0001). Conclusion: This study shows that there is a significant correlation between right heart echocardiographic parameters and concentrations of BNP in the plasma of young adults with ASD. BNP levels may provide a supplemental data to predict of shunt size in these patients. (Echocardiography 2011;28:243‐247)     

组织多普勒成像对正常成人左、右心功能对比观测

目的:探讨组织多普勒成像(tissue Doppler imaging,TDI)技术对正常成人左、右心功能的对比评价。方法:健康成人300例根据年龄分为6组,经胸超声心动图检查,采用心尖四腔切面,应用TDI技术,将取样点置于左室侧壁二尖瓣瓣环处及右室侧壁三尖瓣瓣环处,记录心肌运动速度频谱,测量收缩期峰值(Vs)、舒张早期运动速度峰值(Ve)、舒张晚期运动速度峰值(Va)及Ve/Va比值。结果:二、三尖瓣环处Ve/Va与年龄呈负相关(r1=-0.946,P0.01及r2=-0.925,P0.01),Vs值与年龄不相关;随着年龄增加,舒张早期Ve逐渐减低,Va逐渐增高,二者比值逐渐出现倒置,尤其三尖瓣瓣环出现更早,50岁左右出现,而二尖瓣环处比值倒置在60岁以后。结论:随着年龄增长舒张功能逐渐减低,并且右心室舒张功能减低早于左心室,收缩功能与年龄不相关。     

血清超敏C反应蛋白水平与急性脑梗死的相关性分析

目的探讨血清超敏C反应蛋白（hs-CRP）水平与急性脑梗死的相关性。方法100例72小时内的急性脑梗死病人分为A组（分脑血栓形成、脑栓塞、腔隙性脑梗死三个亚组），80例健康对照组为B组，分别测入院当天、1月后的hs-CRP水平，进行神经功能缺损评分（NIHSS）并两者比较，测A组病人入院当天的血糖、血脂、血压、D-二聚体水平，计算梗塞面积，并与hs-CRP水平进行相关性分析。结果（1）急性期脑梗死组的hs-CRP水平高于对照组（P〈0.0001）；急性期三亚组之间差异也有统计学意义（P〈0.0001），其中脑栓塞组hs-CRP水平最高，1月后仅栓寒组与腔梗组hs-CRP水平之间的差异具有统计学意义（P=0.028）；（2）脑梗死急性期hs-CRP水平与急性期NIHSS评分、梗塞面积、血糖、甘油三脂、低密度脂蛋白、D-二聚体、收缩压水平呈正相关，与高密度脂蛋白水平、1月后NIHSS评分下降百分比呈负相关（P〈0.0001）。其中NIHSS评分、D-二聚体、血糖三项可进入回归方程。结论麻清hs-CRP水平与急性脑梗死的发生、发展相关，与血糖、甘油三脂、高密度脂蛋白、低密度脂蛋白、收缩压等血管性危险因子及D-二聚体密切相关。     

Plasma levels of the soluble fraction of tumor necrosis factor receptors 1 and 2 are independent determinants of plasma cholesterol and LDL-cholesterol concentrations in healthy subjects.

In the last few years, it has been demonstrated that tumor necrosis alpha (TNF-alpha) has important effects on whole-body lipid metabolism. TNF-alpha administration has been found to produce an increase in serum cholesterol levels and increased hepatic hydro-3-methyl-glutaryl coenzyme A (HMG-CoA) reductase activity in mice. The purpose of this study was to test whether plasma levels of the soluble forms of the TNF-alpha receptors 1 and 2 (sTNFR1, sTNFR2) are associated with lipid abnormalities. A total of 36 healthy subjects (19 males, mean age 36.2 +/- 1.9, and 17 females, mean age 34.9 +/- 1.4) were studied. Plasma sTNFR1 levels correlated with total (r = 0.43, P = 0.01) and LDL-cholesterol (r = 0.52, P = 0.002) levels, but not with total or HDL2-HDL3 subfractions of HDL-cholesterol, total plasma triglycerides, VLDL-cholesterol or VLDL-triglycerides (all r < 0.11, P = NS). Plasma sTNFR2 levels also correlated with total (r = 0.44, P = 0.009) and LDL-cholesterol (r = 0.57, P < 0.0001) levels, and negatively with HDL2-cholesterol (r = -0.37, P = 0.029). A stepwise multiple linear regression was constructed to predict total cholesterol levels, with BMI, sex, age, sTNFR1 or sTNFR2 as independent variables. Both sTNFR1 and sTNFR2 were significantly associated with total cholesterol (P = 0.031 and 0.009), contributing to 26 and 19%, respectively, of its variance. In another model in which LDL-cholesterol was substituted for total cholesterol, sTNFR1 or sTNFR2 (P = 0.0084 and 0.0005) were significantly associated with LDL-cholesterol, contributing to 39 and 32% of its variance. In summary, plasma levels of sTNFR1 and sTNFR2 circulate in proportion to total and LDL-cholesterol in healthy subjects.     

Age-related changes in plasma lipid levels and tissue lipoprotein lipase activities of Fischer-344 rats

Fischer-344 rats of 6, 12, 18, 21 and 24 mth of age were investigated. The data were collected from groups of rats ranging from 5-10 rats per age group. Modest age-related increases were noted in body weight, plasma triglycerides, total cholesterol and free (unesterified) cholesterol. Much more significant changes were noted in tissue lipoprotein lipase activities where the adipose tissue lipases declined sharply (r = -0.90, P less than 0.0001; r = -0.86, P less than 0.0001) and the muscle tissue lipases decreased moderately (r = -0.86, P less than 0.0001; r = -0.58, P less than 0.0001) with age. These observed trends suggest that enzymatic parameters, specifically tissue lipoprotein lipase activities, may be considerably more accurate indicators of age-related physiological changes than levels of plasma lipids such as cholesterol and triglyceride.     

Serum adiponectin and leptin levels in relation to the metabolic syndrome, androgenic profile and somatotropic axis in healthy non-diabetic elderly men

OBJECTIVE: The relationships between adipocytokines, sex steroids and the GH/IGF-I axis is poorly studied and subject to controversy in healthy elderly male subjects. We investigated the association between both adiponectin and leptin, and the metabolic syndrome (MetS), lipid parameters, insulin sensitivity, sex steroids and IGF-I in healthy non-diabetic Lebanese men. DESIGN AND METHODS: In this cross-sectional study, a total of 153 healthy non-diabetic men aged 50 and above (mean age 59.3 +/- 7 years) had a detailed clinical and biological evaluation. Subjects were classified according to the National Cholesterol Education Program criteria of the MetS. Insulin sensitivity was determined by the Quantitative Insulin Sensitivity Check Index (QUICKI). RESULTS: Subjects with the MetS had lower adiponectin and higher leptin levels (P < 0.0001 for both variables) compared with individuals without the MetS. Adiponectin was significantly correlated with waist size, triglycerides, high-density lipoprotein (HDL) cholesterol and QUICKI (r = -0.33, -0.26, 0.45 and 0.36 respectively, P < 0.0001 for all variables). The relation between adiponectin and HDL cholesterol, triglycerides and QUICKI remained significant after adjustment for age and body mass index (BMI). Also, leptin was strongly correlated with waist size and QUICKI (r = 0.63 and -0.63 respectively, P < 0.001 for both variables). However, its relation to the lipid profile was weak (for cholesterol r = 0.16, P < 0.05; for triglycerides r = 0.17, P < 0.05) and disappeared after adjustment for BMI. Adiponectin was positively correlated with sex hormone-binding globulin (SHBG) (r = 0.39, P < 0.001) and inversely correlated with free-androgen index (r = -0.24, P < 0.01), estradiol and dehydroepiandrosterone sulfate (r = -0.165, P < 0.05; r = -0.21, P < 0.01 respectively). This difference remained significant for SHBG after adjustment for age and BMI (r = 0.20, P < 0.005). Finally, leptin was inversely correlated with total testosterone and SHBG (r = -0.44, P < 0.001; r = -0.30, P < 0.001 respectively); the relation with testosterone remained significant after adjustment for BMI. No significant relationship of either adiponectin or leptin with GH or IGF-I values was observed. In a stepwise multiple regression analysis, the independent predictors of adiponectin were HDL cholesterol, QUICKI, age and BMI (P < 0.0001, P = 0.005, P = 0.002 and P = 0.047 respectively) while for leptin, it was QUICKI, waist size and testosterone (P < 0.0001, P < 0.0001 and P = 0.004 respectively). The adjusted R2 values were 0.34 and 0.55. CONCLUSION: Our results show that in a healthy elderly male population, both adiponectin and leptin are related to insulin sensitivity, independent of age and BMI. While adiponectin is independently related to triglycerides and HDL cholesterol, the weak relationship of leptin to the lipid profile is completely mediated by BMI. In addition, and more interestingly, both adipocytokines are strongly associated with sex steroids. We speculate that SHBG is regulated by adiponectin and that there is an inhibitory effect of testosterone on the adiponectin gene. Further studies are needed to fully elucidate these relationships.     

Aortic pulse wave velocity is associated with the presence and quantity of coronary artery calcium: a community-based study

We investigated the relationship of aortic pulse wave velocity (aPWV), a measure of central arterial stiffness, with the presence and quantity of coronary artery calcium (CAC) in a community-based sample of adults without prior history of heart attack or stroke (n=401, mean age 59.8 years, 53% men). ECG-gated waveforms of the right carotid and right femoral artery were obtained by applanation tonometry, and aPWV was calculated using established methods. CAC was measured noninvasively by electron beam computed tomography, and CAC score was calculated. aPWV was significantly correlated with log(CAC +1; r=0.41; P<0.0001) and pulse pressure (r=0.47; P<0.0001). Multivariable logistic and linear regression models were used to identify independent predictors of the presence and quantity of CAC, respectively. In multivariable logistic regression analyses, aPWV was associated with the presence of CAC (P=0.011) after adjustment for age, male sex, total cholesterol, high-density lipoprotein cholesterol, diabetes, history of smoking, systolic blood pressure, body mass index, and use of hypertension and statin medications. In multivariable linear regression analyses, aPWV was significantly associated with log(CAC +1) after adjustment for the covariates enumerated above (P<0.0001). aPWV remained significantly associated with both the presence and quantity of CAC even after the additional adjustment for diastolic blood pressure. We conclude that aPWV is related to subclinical coronary atherosclerosis independent of conventional risk factors (including indices of blood pressure) and may be a biomarker of cardiovascular risk in asymptomatic individuals.