| miR-153下调FOXR2、CDK8和CDK13的表达抑制胶质母细胞瘤细胞增殖 |
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| 引用本文： | 胡克琦,周达全,陈,锋,周,毅,敖祥生.miR-153下调FOXR2、CDK8和CDK13的表达抑制胶质母细胞瘤细胞增殖[J].中国临床神经外科杂志,2020,0(11):767-770. |
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| 作者姓名： | 胡克琦 周达全 陈 锋 周 毅 敖祥生 |
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| 作者单位： | 441021 湖北，襄阳市中心医院神经外科（胡克琦、周达全、陈 锋、周 毅、敖祥生） |
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| 摘 要： | 目的 探讨miR-153对胶质母细胞瘤细胞增殖的影响。方法 体外培养胶质母细胞瘤细胞系U87、U251、SHG-44和T98G细胞，分别转染miR-153质粒（miR-153组）、空载质粒（载体组）和miR-153突变质粒（miR-153突变组），另设置空白对照组（不转染任何质粒）。RT-PCR检测miR-153、FOXR2、CDK8和CDK13的表达，MTT法检测细胞增殖能力。结果 miR-153组U87、U251、SHG-44和T98G细胞miR-153表达水平较载体组和空白对照组显著上升（P<0.05），细胞增殖水平较载体组和空白对照组均显著降低（P<0.05）。miR-153组U87、U251、SHG-44和T98G细胞FXOR2、CDK8和CDK13的mRNA水平较miR-153突变组、载体组和空白对照组均显著下降（P<0.05），而后三组之间均无统计学差异（P>0.05）。结论 miR-153抑制胶质母细胞瘤细胞增殖，其机制可能与抑制FXOR2、CDK8和CDK13表达有关。
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| 关 键 词： | 胶质母细胞瘤 微小核糖核酸 miR-153 细胞增殖 |
miR-153 inhibits proliferation of glioblastoma multiforme cell lines by down-regulation of FOXR2, CDK8 and CDK13 |
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| HU Ke-qi,ZHOU Da-quan,CHEN Feng,ZHOU Yi,AO Xiang-sheng..miR-153 inhibits proliferation of glioblastoma multiforme cell lines by down-regulation of FOXR2, CDK8 and CDK13[J].Chinese Journal of Clinical Neurosurgery,2020,0(11):767-770. |
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| Authors: | HU Ke-qi ZHOU Da-quan CHEN Feng ZHOU Yi AO Xiang-sheng |
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| Affiliation: | Department of Neurosurgery, Xiangyang Center Hospital, Xiangyang 441021, China |
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| Abstract: | Objective To study the effect of miR-153 on the cell proliferation of glioblastoma multiforme (GBM) cell lines and its mechanism. Methods GBM cell lines U87, U251, SHG-44 and T98G were cultured in vitro and transfected with miR-153 over-expression plasmid, vector and miR-153 mutant plasmid, respectively. RT-PCR was used to determine the mRNA level of FOXR2, CDK8 and CDK13. MTT assay was used to determine the proliferation of U87, U251, SHG-44 and T98G cells. Results The level of miR-153 and the mRNA level of FOXR2, CDK8 and CDK13 were significantly up-regulated in U87, U251, SHG-44 and T98G cells (P<0.05), and the proliferation of U87, U251, SHG-44 and T98G cells were inhibited after transfected with miR-153 over-expression (P<0.05). Conclusion miR-153 inhibits proliferation of glioblastoma multiforme cell lines, which may be related to the down-regulation of FOXR2, CDK8 and CDK13. |
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| Keywords: | Glioblastoma multiforme miR-153 Cell proliferation |
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