Rutaecarpine attenuates osteoclastogenesis by impairing macrophage colony stimulating factor and receptor activator of nuclear factor κ‐B ligand‐stimulated signalling pathways |
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| Authors: | Yutaka Fukuma Eiko Sakai Shunsuke Komaki Kazuhisa Nishishita Kuniaki Okamoto Takayuki Tsukuba |
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| Affiliation: | 1. Department of Dental Pharmacology, Institute of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Department of Dental Pharmacology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Okayama, Japan |
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| Abstract: | Rutaecarpine is a major alkaloid isolated from Evodia rutaecarpa. Here, we investigated the effects of rutaecarpine on osteoclast differentiation induced by macrophage colony stimulating factor (M‐CSF) and receptor activator of nuclear factor κ‐B ligand (RANKL) in bone marrow‐derived macrophages (BMMs). Treatment with rutaecarpine significantly inhibited osteoclastogenesis and prevented bone resorption of BMM‐derived osteoclasts. Mechanistically, rutaecarpine decreased the protein level of nuclear factor of activated T cells cytoplasmic‐1 (NFATc1) and the phosphorylation of other signalling pathways during the osteoclast differentiation. Thus, rutaecarpine may be useful as a therapeutic agent for the treatment of bone diseases. |
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| Keywords: | bone marrow‐derived macrophages osteoclast differentiation rutaecarpine |
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