| 二苯乙烯苷对大鼠主动脉舒张作用及其一氧化氮含量的影响 |
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| 引用本文： | 刘其礼,班翊,张俊红,叶少剑,王嘉陵.二苯乙烯苷对大鼠主动脉舒张作用及其一氧化氮含量的影响[J].中国药理学与毒理学杂志,2004,18(4):289-293. |
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| 作者姓名： | 刘其礼 班翊 张俊红 叶少剑 王嘉陵 |
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| 作者单位： | 华中科技大学同济医学院药理学系,湖北,武汉,430030 |
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| 摘 要： | 目的 研究中药何首乌中有效成分二苯乙烯苷 (2 ,3,5 ,4′ 四羟基二苯乙烯 2 O β D 葡萄糖苷 ,THSG)与结构相似的白黎芦醇 (resveratrol)是否有类似的血管舒张作用。方法 采用血管张力记录法及NO比色法 ,观察THSG对大鼠主动脉环张力及NO含量的影响。结果 ①在去氧肾上腺素预收缩的内皮完整及去内皮主动脉环上 ,THSG 0 .1～ 30 .0 μmol·L- 1浓度依赖性舒张主动脉环 ,其Emax分别为 75 %和5 2 % ,EC50 分别为 0 .86和 2 .70 μmol·L- 1。②在内皮完整的血管上 ,THSG 0 .1μmol·L- 1能增强乙酰胆碱1μmol·L- 1依血管内皮的舒张作用。③NO前体物L 精氨酸 1μmol·L- 1可增强THSG 1μmol·L- 1的血管舒张作用 ;而NOS抑制药Nω 硝基L 精氨酸甲酯0 .5 μmol·L- 1可部分削弱之。④鸟苷酸环化酶抑制药亚甲兰 1μmol·L- 1可部分削弱THSG 1μmol·L- 1的血管舒张作用。⑤THSG 30mg·kg- 1,iv ,可短暂降低麻醉大鼠动脉血压 ,但对心率无明显影响。⑥THSG 1μmol·L- 1显著提高大鼠胸主动脉血管组织NO含量。结论 THSG对大鼠主动脉具有舒张作用 ,此作用主要是依血管内皮的 ,并主要由NO介导
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| 关 键 词： | 二苯乙烯苷 主动脉 血管舒张 一氧化氮 |
| 收稿时间： | 2003-10-21 |
Influence of 2,3,5,4'-tetrahydroxystilbene-2- O-β-D-glucoside on vasodilation and nitric oxide content in rat aorta |
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| LIU Qi-Li, BAN Yi, ZHANG Jun-Hong, YE Shao-Jian, WANG Jia-Ling.Influence of 2,3,5,4''''-tetrahydroxystilbene-2- O-β-D-glucoside on vasodilation and nitric oxide content in rat aorta[J].Chinese Journal of Pharmacology and Toxicology,2004,18(4):289-293. |
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| Authors: | LIU Qi-Li BAN Yi ZHANG Jun-Hong YE Shao-Jian WANG Jia-Ling |
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| Affiliation: | LIU Qi-Li*, BAN Yi, ZHANG Jun-Hong, YE Shao-Jian, WANG Jia-Ling |
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| Abstract: | AIM To investigate if 2,3,5,4′-tetrahydroxystilbene-2-O-β-D- glucoside (THSG) has similar vasodilation as its structure analog resveratrol on the aorta. METHODS The vascular tone of isolated rat aortic rings with intact endothelium or denuded endothelium was recorded and the content of NO of aortic rings with intact endothelium was measured. RESULTS ① THSG 0.1－30.0 μmol•L-1 concentratio-dependently antagonized the phenylephrine-induced vasocontraction in both intact-endothelium and endothelium-denuded rat aorta with the Emax of 75% and 52%, and the EC50 value of 0.86 and 2.70 μmol•L-1, respectively. ② In rat aortic rings with intact endothelium, THSG 0.1 μmol•L-1 reinforced the aortic relaxation induced by acetylcholine 1 μmol•L-1. ③ L-Arginine, a NO precursor, 1 μmol•L-1 potentiated the relaxing effect of THSG 1 μmol•L-1. Moreover, Nω-nitro-L-arginine methyl ester(L-NAME), a NOS-inhibitor, 0.5 μmol•L-1 partially decreased the aortic relaxation induced by THSG 1 μmol•L-1. ④ Methylthioninium chloride, an inhibitor of soluble guanyl cyclase, 1 μmol•L-1 also partially extenuated the aortic relaxation effect evoked by THSG 1 μmol•L-1. ⑤ THSG 30 mg•kg-1，iv, temporarily decreased arterial blood pressure of anesthetized rats, but the heart rate was not obviously changed. ⑥ THSG 1 μmol•L-1 significantly enhanced the NO content of endothelium-intact aortic rings. CONCLUSION THSG possesses the vasodilator effect in vivo and in vitro, which partly depends on endothelium and is mediated by NO. |
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| Keywords: | 2 3 5 4′-tetrahydroxystilbene-2-O-β-D-glucoside aorta vasodilation nitric oxide |
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