Diesel exhaust particulates affect cell signaling,mucin profiles,and apoptosis in trachea explants of Balb/C mice |
| |
Authors: | Robson Seriani Mara de Souza Junqueira Alessandra Choqueta de Toledo Milton Arruda Martins Marcelo Seckler Adriano Mesquita Alencar Elnara Marcia Negri Luiz Fernando Ferraz Silva Thaís Mauad Paulo Hilário Nascimento Saldiva Mariangela Macchione |
| |
Affiliation: | 1. Laboratory of Experimental Air Pollution, Department of Pathology, School of Medicine, University of S?o Paulo, S?o Paulo, SP, Brazil;2. Central Biotery Laboratory, School of Medicine, University of S?o Paulo, S?o Paulo, SP, Brazil;3. Experimental Therapeutics Laboratory, Department of Medicine, School of Medicine, University of S?o Paulo, S?o Paulo, SP, Brazil;4. Department of Chemistry Engineering, Polytechnic School, University of S?o Paulo, S?o Paulo, SP, Brazil;5. Department of General Physics ‐ Institute of Physics, University of S?o Paulo, S?o Paulo, SP, Brazil |
| |
Abstract: | Particulate matter from diesel exhaust (DEP) has toxic properties and can activate intracellular signaling pathways and induce metabolic changes. This study was conducted to evaluate the activation of extracellular signal‐regulated kinase (ERK) and c‐Jun N‐terminal kinase (JNK) and to analyze the mucin profile (acid (AB+), neutral (PAS+), or mixed (AB/PAS+) mucus) and vacuolization (V) of tracheal explants after treatment with 50 or 100 μg/mL DEP for 30 or 60 min. Western blot analyses showed small increases in ERK1/2 and JNK phosphorylation after 30 min of 100 μg/mL DEP treatment compared with the control. An increase in JNK phosphorylation was observed after 60 min of treatment with 50 μg/mL DEP compared with the control. We did not observe any change in the level of ERK1/2 phosphorylation after treatment with 50 μg/mL DEP. Other groups of tracheas were subjected to histological sectioning and stained with periodic acid‐Schiff (PAS) reagent and Alcian Blue (AB). The stained tissue sections were then subjected to morphometric analysis. The results obtained were compared using ANOVA. Treatment with 50 μg/mL DEP for 30 min or 60 min showed a significant increase (p < 0.001) in the amount of acid mucus, a reduction in neutral mucus, a significant reduction in mixed mucus, and greater vacuolization. Our results suggest that compounds found in DEPs are able to activate acid mucus production and enhance vacuolization and cell signaling pathways, which can lead to airway diseases. © 2014 Wiley Periodicals, Inc. Environ Toxicol 30: 1297–1308, 2015. |
| |
Keywords: | histopathology DEP biomarkers signal transduction explants MAPK |
|
|