| 新诊断2型糖尿病患者血清非甲基化胰岛素DNA水平的意义及其影响因素 |
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| 引用本文： | 王少波,包利文,尹丽明,邓黎,付文金.新诊断2型糖尿病患者血清非甲基化胰岛素DNA水平的意义及其影响因素[J].国际医药卫生导报,2022,28(9):1222-1226. |
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| 作者姓名： | 王少波 包利文 尹丽明 邓黎 付文金 |
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| 作者单位： | 1东莞市厚街医院内分泌科，东莞 523945；
2东莞市厚街医院中心实验室，东莞 523945 |
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| 基金项目： | 2020东莞市社会科技发展重点项目（202050715023196） |
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| 摘 要： | 目的 用病例对照研究血非甲基化胰岛素DNA（INSDNA）在新诊断2型糖尿病（NT2DM）中的意义及影响因素。方法 选择2018年10月至2020年5月在东莞市厚街医院内分泌科住院的NT2DM患者45例，其中男30例、女15例，年龄（47.0±9.6岁）；长病程2型糖尿病（LT2DM）患者23例，其中男15例、女8例，年龄（48.6±8.6岁）；糖耐量正常（NGT）20例，其中男13例、女7例，年龄（40.2±5.5岁）。用液滴数字PCR技术（ddPCR）检测血清非甲基化INSDNA及ELSA检测人组织蛋白酶S（Cath-S）和白介素6（IL-6）水平，采用单因素方差分析各组非甲基化INSDNA水平差异及用Spearman相关分析空腹C肽（FC）、Cath-S、IL-6与非甲基化INSDNA水平的相关性。统计学方法采用独立样本t检验、χ2检验。结果 NT2DM组患者的血清非甲基化INSDNA水平明显高于NGT组和LT2DM组［3.01（4.64）copies/μl比0.59（0.19）copies/μl、1.77（2.80）copies/μl］，差异均有统计学意义（均P<0.01）。NT2DM组的患者Cath-S水平［683.37（283.90）nmol/L比479.70（111.30）nmol/L］、IL-6水平［28.95（9.99）pg/ml比3.42（6.90）pg/ml）］均明显高于NGT组（均P<0.01）。相关分析显示，非甲基化INSDNA水平在NT2DM患者与Cath-S、IL-6和FC呈正相关（r=0.354，P=0.017；r=0.320，P=0.032；r=0.434，P=0.003）。结论 血清非甲基化INSDNA水平在NT2DM疾病早期水平较高，随着病程延长而降低，血Cath-S、IL-6和FC可能是影响NT2DM血清非甲基化INSDNA水平的重要因素。
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| 关 键 词： | 血清非甲基化INSDNA 新诊断T2DM Cath-S IL-6 |
| 收稿时间： | 2021-12-07 |
Significance and influencing factors of serum level of ummethylation INSDNA in patients with newly diagnosed type 2 diabetes mellitus |
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| Wang Shaobo,Bao Liwen,Yin Liming,Deng Li,Fu Wenjin.Significance and influencing factors of serum level of ummethylation INSDNA in patients with newly diagnosed type 2 diabetes mellitus[J].International Medicine & Health Guidance News,2022,28(9):1222-1226. |
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| Authors: | Wang Shaobo Bao Liwen Yin Liming Deng Li Fu Wenjin |
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| Affiliation: | 1 Department of Endocrinology, Dongguan Houjie Hospital, Dongguan
523945, China;
2 Central Laboratory, Dongguan Houjie Hospital,
Dongguan 523945, China |
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| Abstract: | Objective To investigate the significance and influencing factors of serum level
of ummethylation insulin DNA (INSDNA) in patients with newly diagnosed type 2
diabetes mellitus (NT2DM) by case control study. Methods Forty-five patients with NT2DM hospitalized at Department of
Endocrinology, Dongguan Houjie Hospital from October 2018 to May 2020 were
selected, including 30 males and 15 females, and they were (47.0±9.6) years
old. There were 23 patients with long course T2DM (LT2DM), including 15 males and
8 females, and they were (48.6±8.6) years old. There were 20 patients with
normal glucose tolerance (NGT), including 13 males and 7 females, and they were
(40.2±5.5) years old. The serum level of unmethylated INSDNA was detected by
drop digital polymerase chain reaction (ddPCR), and the levels of human
cathepsin S (Cath-S) and interleukin 6 (IL-6) by enzyme linked immunosorbent
assay (ELISA). One-way ANOVA was used to analyze the difference in the
unmethylated INSDNA level between the three groups, and Spearman correlation
analysis was used to analyze the correlation of unmethylated INSDNA level with
fasting C peptide (FC), Cath-S, and IL-6. Independent-sample t test and χ2 test were applied. Results The serum level of unmethylated INSDNA in the patients with NT2DM was
significantly higher than those in the patients with NGT and the patients with
LT2DM 3.01 (4.64) copies/μl vs. 0.59 (0.19) copies/μl and 1.77 (2.80)
copies/μl], with statistical differences (both P<0.01). The levels of Cath-S and IL-6 in the patients with
NT2DM were significantly higher than those in the patients with NGT 683.37
(283.90) nmol/L vs. 479.70 (111.30) nmol/L and 28.95 (9.99) pg/ml vs. 3.42
(6.90) pg/ml; both P<0.01]. The
correlation analysis showed that the level of unmethylated INSDNA was
positively correlated with the levels of FC, Cath-S, and IL-6 in the patients
with NT2DM (r=0.354, P=0.017; r=0.320, P=0.032; r=0.434, P=0.003). Conclusions The serum level of
unmethylated INSDNA is higher in patients with T2DM during early stage and
decreases as the disease course prolongs. Serum Cath-S, IL-6, and FC may be
important factors affecting the level of unmethylated INSDNA. |
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| Keywords: | Serum unmethylated INSDNA Newly diagnosed T2DM Cath-S IL-6 |
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