| 高脂血症apoE基因敲除小鼠心脏中一氧化氮合酶含量及基因表达 |
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| 引用本文： | 曹晓娥,李晓波,殷莲华.高脂血症apoE基因敲除小鼠心脏中一氧化氮合酶含量及基因表达[J].复旦学报(医学版),2009,36(4):403-406. |
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| 作者姓名： | 曹晓娥 李晓波 殷莲华 |
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| 作者单位： | 1. 上海职工医学院生理教研室,上海,200237
2. 复旦大学上海医学院生理学与病理生理学系,上海,200032 |
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| 摘 要： | 目的 探讨高脂饲料对apoE基因敲除(apoE-/-)小鼠心肌组织中内皮型一氧化氮合酶（endothelial nitric-oxide synthase，eNOS）的含量及基因表达的影响。方法 将13只apoE-/-小鼠随机分为两组，即高脂饲料组（n=7）和普通饲料组（n=6）。喂养12周后，测血清总胆固醇（total cholesterol，TC）、三酰甘油（triglyceride，TG）、高密度脂蛋白（high density lipoprotein，HDL）和低密度脂蛋白（low density lipoprotein，LDL）含量，采用Real-Time RT-PCR法检测心肌组织中eNOS的mRNA表达水平，以免疫组化方法检测eNOS蛋白的表达。结果 高脂饲料组TC、TG和LDL明显高于普通饲料组，HDL则低于普通饲料组（P<0.01）；高脂饲料组心肌组织中eNOS的mRNA和蛋白水平均低于普通饲料组（P<0.01）。结论 高脂饲料导致apoE-/-小鼠发生高脂血症，并引起心肌组织中eNOS的mRNA表达的减少和相应蛋白水平的降低。
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| 关 键 词： | 高脂血症 内皮型一氧化氮合酶 apoE-/-小鼠 |
| 收稿时间： | 2008-11-18 |
Content and gene expression of eNOS in heart of hyperlipidemia apoE-/- mice |
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| CAO Xiao-e,LI Xiao-bo,YIN Lian-hua.Content and gene expression of eNOS in heart of hyperlipidemia apoE-/- mice[J].Fudan University Journal of Medical Sciences,2009,36(4):403-406. |
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| Authors: | CAO Xiao-e LI Xiao-bo YIN Lian-hua |
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| Affiliation: | 1Department of Physiology;Shanghai Medical Workers College;Shanghai 200237;China;2Department of Physiology and Pathophysiology;Shanghai Medical College;Fudan University;Shanghai 200032;China |
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| Abstract: | Objective To explore the content and gene expression of endothelial nitric-oxide synthase (eNOS) in heart of hyperlipidemia apoE-/-mice. Methods apoE-/-mice were fed with high fat diet as high-fat diet group (n=7) and normal fat diet as control group (n=6) for 12 weeks. Serum lipid profiles including total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected. eNOS mRNA expression in heart were determined by Real-Time RT-PCR,as well as protein expr... |
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| Keywords: | hyperlipidemia eNOS apoE-/-mice |
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