首页 | 官方网站   微博 | 高级检索  
     

miR-638对人肺腺癌细胞凋亡的影响
引用本文:曹 艳,王 鹏,娄鉴芳,李大千,吴 蕾,陈 丹,谢而付,顾 兵,徐华国,王 芳,徐 建,潘世扬.miR-638对人肺腺癌细胞凋亡的影响[J].南京医科大学学报,2014(3):287-290.
作者姓名:曹 艳  王 鹏  娄鉴芳  李大千  吴 蕾  陈 丹  谢而付  顾 兵  徐华国  王 芳  徐 建  潘世扬
作者单位:南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029;南京医科大学第一附属医院检验学部,江苏 南京 210029
基金项目:国家自然科学基金(81371894,81302531, 81272324,81201359,81101322);江苏省实验诊断学重点实验室(XK201114);江苏高校优势学科建设工程基金项目;教育部博导基金(20113234110012);国家临床重点专科建设项目
摘    要:目的:探讨miR-638在肺腺癌中的表达及其与肺腺癌细胞凋亡的关系?方法:应用real-time RT-PCR的方法检测了miR-638在肺腺癌细胞株SPC-A1中的表达情况,并采用脂质体法将miR-638 模拟物瞬时转染入SPC-A1?实验设置空白对照组?无关miRNA阴性对照组和miR-638转染组,转染后在荧光显微镜下观察转染效率,实时荧光定量RT-PCR检测miR-638的表达,流式细胞术检测各组细胞凋亡率?结果:与正常细胞相比,miR-638在肺腺癌细胞中低表达?SPC-A1细胞转染miR-638模拟物后,细胞凋亡率相对于空白组和阴性对照组显著升高(P < 0.05)?结论:miR-638在肺腺癌中低表达,且能够促进肺腺癌细胞凋亡,可作为后续肺癌生物治疗的新分子靶标?

关 键 词:凋亡  miR-638  肺腺癌
收稿时间:2013/12/19 0:00:00

Impact of miR-638 on apoptosis of lung adenocarcinoma cells
Cao Yan,Wang Peng,Lou Jianfang,Li Daqian,Wu lei,Chen Dan,Xie Erfu,Gu Bing,Xu Huaguo,Wang Fang,Xu Jian and Pan Shiyang.Impact of miR-638 on apoptosis of lung adenocarcinoma cells[J].Acta Universitatis Medicinalis Nanjing,2014(3):287-290.
Authors:Cao Yan  Wang Peng  Lou Jianfang  Li Daqian  Wu lei  Chen Dan  Xie Erfu  Gu Bing  Xu Huaguo  Wang Fang  Xu Jian and Pan Shiyang
Affiliation:Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China;Department of Laboratory Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China
Abstract:Objective:To investigate expression of miR-638 and effects of miR-638 on apoptosis of lung adenocarcinoma cell line(SPC-A1). Methods:The expression of miR-638 in lung adenocarcinoma cell line was detected by real-time RT-PCR. Mimics of mir-638 were transiently transfected into SPC-A1 by using lipofectamine method. SPC-A1 cells were transfected with miR-638 mimics or non-special oligonucleotides(as negative control)or nothing(as blank control). After transfection,transfection efficiency was observed by fluorescence microscope. MiR-638 levels were detected by real-time quantitative RT-PCR. Cell apoptosis was detected by flow cytometry. Results:We observed that miR-638 was significantly inhibited in lung adenocarcinoma cells compared with that in normal cells. Cell apoptosis rate was increased significantly in cells transfected with miR-638(P <0.05)compared with negative and blank control cells. Conclusion:MiR-638 was poorly expressed in lung adenocarcinoma cells,and it could dramatically promote apoptosis of lung adenocarcinoma cells,thus provides a new target for the use of miR-638 in lung cancer biotherapy.
Keywords:apoptosis  miR-638  lung adenocarcinoma
本文献已被 CNKI 等数据库收录!
点击此处可从《南京医科大学学报》浏览原始摘要信息
点击此处可从《南京医科大学学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号