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PTH通过Notch信号通路促进骨髓间充质干细胞向成骨细胞分化
引用本文:王维东,万博文,周灵杰,凡 进,殷国勇.PTH通过Notch信号通路促进骨髓间充质干细胞向成骨细胞分化[J].南京医科大学学报,2015(11):1517-1521.
作者姓名:王维东  万博文  周灵杰  凡 进  殷国勇
作者单位:南京医科大学第一附属医院骨科,江苏 南京 210029,南京医科大学第一附属医院骨科,江苏 南京 210029,南京医科大学第一附属医院骨科,江苏 南京 210029,南京医科大学第一附属医院骨科,江苏 南京 210029,南京医科大学第一附属医院骨科,江苏 南京 210029
基金项目:国家自然科学基金(81271988)
摘    要:

关 键 词:PTH  Notch  骨髓间充质干细胞  成骨分化
收稿时间:2015/2/27 0:00:00

PTH induces differentiation of bone mesenchymal stem cells into osteoblasts through Notch signaling pathway
Wang Weidong,Wan Bowen,Zhou Lingjie,Fan Jing and Yin Guoyong.PTH induces differentiation of bone mesenchymal stem cells into osteoblasts through Notch signaling pathway[J].Acta Universitatis Medicinalis Nanjing,2015(11):1517-1521.
Authors:Wang Weidong  Wan Bowen  Zhou Lingjie  Fan Jing and Yin Guoyong
Affiliation:Department of Orthopedics,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Orthopedics,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Orthopedics,the First Affiliated Hospital of NJMU,Nanjing 210029,China,Department of Orthopedics,the First Affiliated Hospital of NJMU,Nanjing 210029,China and Department of Orthopedics,the First Affiliated Hospital of NJMU,Nanjing 210029,China
Abstract:Objective:To study whether parathyroid hormone regulates the differentiation of bone mesenchymal stem cells into osteoblasts through Notch signaling pathway. Methods:Six PTH+/+ and six PTH-/- mice of 8-week-old were used in this study. Bone mesenchymal stem cells obtained from mouse femurs were isolated and cultured,then osteogenic differentiation was induced. Western blot was performed to detect the expression of Notch signaling pathway at day 3,7,10,14. The alkaline phosphate (ALP)activity was detected to evaluate the early osteogentic differentiation at day 3 and 7. Western blot was performed to detect the expression of Runx2 and OCN proteins. Results:The receptor and ligand of Notch signal pathway and the osteogenic index was increased in the wild type group and PTH gene knockout group after osteogenic differentiation. The expression of Jagged1 and Notch1 protein in PTH gene knockout group was significantly lower than that of the wild type group (P < 0.01). Compared with the wild type group,Runx2 and OCN protein levels were lower in PTH gene knockout group,with the same trends as ALP results. Conclusion:The deficiency of endogenous PTH inhibited the differentiation of bone marrow mesenchymal stem cells into osteoblasts,by reducing the expression of Notch1 and Jagged1 proteins.
Keywords:PTH  Notch  bone mesenchymal stem cells  osteogenic differentiation
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