| 苦参碱眼液调控TLR4/NF-κB通路影响实验性葡萄膜炎家兔相关细胞因子的表达 |
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| 引用本文： | 邓珍,吴一峰,姜蕾,唐丹燕,俞国孝,姚莉,吴坤林.苦参碱眼液调控TLR4/NF-κB通路影响实验性葡萄膜炎家兔相关细胞因子的表达[J].中华全科医学,2020,18(4):568. |
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| 作者姓名： | 邓珍 吴一峰 姜蕾 唐丹燕 俞国孝 姚莉 吴坤林 |
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| 作者单位： | 杭州市余杭区第一人民医院眼科, 浙江 杭州 311100 |
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| 基金项目： | 杭州市余杭区科学技术局项目(2015011)浙江省医药卫生科技计划项目(2017KY238) |
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| 摘 要： | 目的 探究苦参碱眼液调控Toll样受体4/核因子-κB(TLR4/NF-κB)通路对家兔实验性葡萄膜炎相关细胞因子水平的影响。 方法 建立实验性葡萄膜炎家兔模型,采用随机数字表的方法分为模型组、妥布霉素地塞米松滴眼液组、苦参碱眼液低(0.50 g/L)、中(0.75 g/L)、高(1.00 g/L)剂量组,每组8只,另取8只设为对照组。造模后妥布霉素地塞米松滴眼液组、苦参碱眼液低、中、高剂量组分别以妥布霉素地塞米松滴眼液、苦参碱眼液滴眼治疗,对照组、模型组家兔以相同剂量生理盐水滴眼,持续7 d。裂隙灯观察各组家兔眼前节炎症反应并对其进行临床评分,苏木精-伊红染色(HE)观察各组家兔视网膜组织病理形态并对其进行病理评分,酶联免疫吸附法(ELISA)检测各组家兔血清及房水中细胞因子肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1(IL-1)]水平,蛋白免疫印迹法(Western blotting)检测视网膜组织中TLR4/NF-κB通路蛋白TLR4、MyD88、NF-κB p65的表达。 结果 与对照组相比,模型组家兔临床评分、病理评分,血清和房水中IL-6、IL-1、TNF-α水平,视网膜组织中TLR4、MyD88、NF-κB p65表达均显著升高(均P<0.05);与模型组相比,苦参碱眼液各剂量组和妥布霉素地塞米松滴眼液组家兔临床评分、病理评分,血清和房水中IL-6、IL-1、TNF-α水平,视网膜组织中TLR4、MyD88、NF-κB p65表达均降低且苦参碱眼液各剂量组之间呈剂量依赖性(均P<0.05);苦参碱眼液高剂量组和妥布霉素地塞米松滴眼液组各指标相比,差异无统计学意义(P>0.05)。 结论 苦参碱眼液可降低血清及房水中细胞炎症因子水平,减轻炎症反应,改善家兔葡萄膜炎症状,可能是通过下调TLR4/NF-κB通路实现。
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| 关 键 词： | 苦参碱眼液 家兔 葡萄膜炎 TLR4/NF-κB通路 细胞因子 |
| 收稿时间： | 2019-06-26 |
Effects of matrine eye drops on levels of cytokines associated with experimental uveitis in rabbits by regulating TLR4/NF-κB pathway |
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| Affiliation: | Department of Ophthalmology, the First Peoples Hospital of Yuhang District, Hangzhou, Zhejiang 311100, China |
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| Abstract: | Objective To investigate the effect of matrine eye drops on the levels of cytokines associated with experimental uveitis in rabbits by regulating toll like receptor 4/nuclear factor-κB(TLR4/NF-κB) pathway. Methods The rabbit model of experimental uveitis was established and randomly divided into model group, tobramycin-dexamethasone eye drops group, low(0.50 g/L), medium(0.75 g/L) and high(1.00 g/L) dose matrine eye drops groups, with 8 rabbits in each group. Another 8 rabbits were selected as control group. After successfully modeling, tobramycin-dexamethasone eye drops group, low, medium and high dose matrine eye drops groups were treated with tobramycin-dexamethasone eye drops and matrine eye drops respectively, rabbits in all groups were given the same dose of saline eye drops for 7 days. Slit lamp was used to observe and evaluate the inflammation of anterior segment of rabbit eyes. Hematoxylin-eosin staining(HE) was used to observe the pathological morphology of the retina of rabbits. Enzyme-linked immunosorbent assay(ELISA) was used the levels of cytokines, tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1(IL-1) in serum and aqueous humor of rabbits, and Western blot was used to detect the expressions of TLR4/NF-κB pathway proteins TLR4, MyD88 and NF-κB p65 in retina. Results Compared with the control group, the clinical and pathological scores, the levels of IL-6, IL-1 and TNF-α in serum and aqueous humor, and the expressions of TLR4, MyD88 and NF-κB p65 in retina were significantly increased in the model group(all P<0.05). Compared with model group, the clinical and pathological scores, the levels of IL-6, IL-1 and TNF-α in serum and aqueous humor, and the expressions of TLR4, MyD88 and NF-κB p65 in retina tissue in all dose matrine eye drops groups and tobramycin-dexamethasone eye drops group were decreased, and there was a dose-dependent relationship among the dose groups of matrine eye drops(all P<0.05). There was no significant difference between the high dose matrine eye drops groups and tobramycin-dexamethasone eye drops group(P>0.05). Conclusion Matrine eye drops can reduce the levels of cytokines in serum and aqueous humor, alleviate inflammation and improve uveitis symptoms in rabbits, which may be achieved by down-regulating TLR4/NF-κB pathway. |
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