结直肠癌中差异性表达的外泌体lncRNA功能富集及通路分析 |
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引用本文: | 曾薇,刘翼,李文婷,朱金峰.结直肠癌中差异性表达的外泌体lncRNA功能富集及通路分析[J].中华全科医学,2022,20(1):141. |
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作者姓名: | 曾薇 刘翼 李文婷 朱金峰 |
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作者单位: | 1.新疆医科大学附属肿瘤医院肺内科,新疆 乌鲁木齐 830011 |
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基金项目: | 新疆维吾尔自治区自然科学基金面上项目(2018D01C255)。 |
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摘 要: | 目的 通过探讨差异性表达的外泌体lncRNA及其调控的信号通路在结直肠癌发生、发展中的作用,以期为结直肠癌的诊断和治疗提供新的靶点。 方法 使用R软件线性回归模型软件包limma包进行差异表达分析,根据筛选阈值得到显著差异表达的lncRNA和mRNA;通过计算差异表达lncRNA与mRNA皮尔逊相关性系数,获得其之间协同表达关系,通过相关系数筛选存在正向协同表达的lncRNA-mRNA关系对;分别对lncRNA的协同表达mRNA进行GO功能和KEGG通路富集分析,以间接预测lncRNA的功能。 结果 在32例健康志愿者和12例结直肠癌患者中筛选出差异性表达的外泌体lncRNA共202个、mRNA共1 014个。验证出差异性表达的lncRNA-mRNA关系对10个。对lncRNA协同表达的mRNA进行功能富集显示,差异性表达的外泌体lncRNA可能与B细胞受体信号通路、NF-κB信号通路、JAK-STAT信号通路、抗原处理与呈递信号通路和NOD样受体信号通路活化密切相关。 结论 深入探索外泌体lncRNA在结直肠癌中的差异性表达、功能富集及通路活化,有助于为结直肠癌的治疗提供新思路,为诊断提供新靶点。
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关 键 词: | 结直肠癌 外泌体 长链非编码RNA |
收稿时间: | 2021-02-03 |
Functional enrichment and pathway analysis of differential expression of exosome lncRNA in colorectal cancer |
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Authors: | ZENG Wei LIU Yi LI Wen-ting ZHU Jin-feng |
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Affiliation: | First Department of Lung Cancer Chemotherapy, the Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China |
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Abstract: | Objective To explore the role of differential expression of exosome lncRNA and its regulated signal pathway in the occurrence and development of colorectal cancer and provide a new target for the diagnosis and treatment of colorectal cancer.Methods The linear regression model package limma of R software was used to analyse the differential expression of lncRNA and mRNA.By calculating the Pearson correlation coefficient between differentially expressed lncRNA and mRNA,their co-expression was obtained.The positive co-expression of lncRNA-mRNA was screened by using a correlation coefficient.Go function and KEGG pathway enrichment of mRNA co-expressed with lncRNA were analysed to indirectly predict the function of lncRNA.Results A total of 202 differentially expressed exosome lncRNAs and 1014 exosome mRNA were screened from 32 healthy volunteers and 12 colorectal cancer patients.Ten pairs of lncRNA-mRNA co-expression were verified.Functional enrichment of mRNA co-expressed by lncRNA showed that differentially expressed exosome lncRNA may be closely related to B cell receptor signal pathway,NF-KB signal pathway,JAK-STAT signal pathway,antigen processing and presentation signal pathway and NOD-like receptor signal pathway activation.Conclusion In-depth exploration of differential expression,functional enrichment and pathway activation of exosome lncRNA in colorectal cancer is important to provide new ideas for the diagnosis and treatment of colorectal cancer. |
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Keywords: | Colorectal cancer Exosome Long non-coding RNA |
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