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猴艾滋病急性期肠粘膜相关淋巴组织NK细胞表型及功能
引用本文:吴芳新,王卫,刘克剑,丛喆,赵长城,熊竞,苏爱华,鞠斌,魏强.猴艾滋病急性期肠粘膜相关淋巴组织NK细胞表型及功能[J].中国比较医学杂志,2012(6):37-42.
作者姓名:吴芳新  王卫  刘克剑  丛喆  赵长城  熊竞  苏爱华  鞠斌  魏强
作者单位:中国医学科学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室
基金项目:国家科技重大专项课题(2012ZX10001-007-008和2012ZX10004-501);基本业务费课题(DWS201112)
摘    要:目的研究猴艾滋病毒感染急性期恒河猴肠道相关淋巴组织(mucosal associated lymphoid tissues,MALTs)NK细胞亚群和功能变化。方法 SIV静脉感染恒河猴后,定期进行动物感染指标测定,并在感染后不同时间点取肠组织,分离派氏淋巴结单个核细胞(peyer’s patch mononuclear cells,PPMC)和粘膜固有层单个核细胞(lamina propria mononuclear cells,LPMC),进行T细胞和NK细胞表面抗体染色,流式分析。结果 SIV感染急性期MALTs CD56CD16+NK细胞亚群比例增幅明显,同时细胞毒性功能增强;CD56-CD16-NK细胞亚群数量减少,功能无明显变化;CD56+CD16+和CD56+CD16-NK细胞数量比例略有增加趋势,但免疫调节功能显著降低。结论SIV感染急性期恒河猴肠道MALTs中NK细胞脱颗粒作用增强,表型功能呈现出较强可塑性。该研究对探索艾滋病粘膜免疫机理、抗病毒治疗及药物研发具有参考意义。

关 键 词:SIV感染  恒河猴  肠道粘膜相关淋巴组织  NK细胞  脱颗粒作用

Changes of Phenotype and Functions of NK Cells in Gut Associated Lymphoid Tissues from Rhesus Monkey Infected with SIV during Acute Phase
WU Fang-xin,WANG Wei,LIU Ke-jian,CONG Zhe,ZHAO Chang-cheng,XIONG Jing,SU Ai-hu,JU Bin and WEI Qiang.Changes of Phenotype and Functions of NK Cells in Gut Associated Lymphoid Tissues from Rhesus Monkey Infected with SIV during Acute Phase[J].Chinese Journal of Comparative Medicine,2012(6):37-42.
Authors:WU Fang-xin  WANG Wei  LIU Ke-jian  CONG Zhe  ZHAO Chang-cheng  XIONG Jing  SU Ai-hu  JU Bin and WEI Qiang
Affiliation:(Key Laboratory of Human Diseases Comparative Medicine,Ministry of Health;Institute of Medical Laboratory Animal Science,Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models,State administration of Traditional Chinese medicine,Beijing 100021,China)
Abstract:Objective To study the phenotype and functions of NK cells in mucosal associated lymphoid tissues(MALTs) from rhesus monkeys infected with SIV during acute phase.Methods four rhesus monkeys were inoculated with SIVmac239.Plasma viral load and CD4+/CD8+ ratio were detected at regular intervals.To obtain mucosal associated lymphoid tissues,ileum of animals was collected by operations at different times after inoculation.Then,mononuclear cells from this tissue were stained with antibodies,and detected with flow cytometry.Results CD56-CD16+ in MALTs were expanded in numbers and the degranulation functions were enhanced during acute phase of SIV infection.The ratio of DN NK cells were reduced but their functions showed unchangeable.The ratio of DP NK cells and CD56+CD16-NK cells increased slightly,but the immunoregulation functions were decreased.Conclusions The degranulation cytotoxicity potential of NK cells from gut associated lymphoid tissues(MALTs) from SIV infected rhesus monkey were enhanced during acute phase.This study plays an important role in the exploration of mucosal immunologic mechanism of SIV infection.
Keywords:SIV infection  Rhesus monkey  Mucosal associated lymphoid tissues  NK cells  Degranulation
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