|
|||||
|
|
| 埃他卡林对大鼠肾脏缺血再灌注损伤的保护作用 | |
| 引用本文： | 吕昌迎,龙超良,高俊钰,汪海.埃他卡林对大鼠肾脏缺血再灌注损伤的保护作用[J].国际药学研究杂志,2008,35(2):81-86. |
| 作者姓名： | 吕昌迎 龙超良 高俊钰 汪海 |
| 作者单位： | 1. 军事医学科学院附属医院,北京100071;山东省莱芜市中医医院,山东莱芜271100;军事医学科学院毒物药物研究所,北京100850 2. 军事医学科学院毒物药物研究所,北京,100850 3. 山东省莱芜市中医医院,山东莱芜,271100 |
| 基金项目： | 国家高技术研究发展计划(863计划)重大专项基金资助项目 |
| 摘 要： | 目的 观察埃他卡林对在体大鼠肾脏缺血再灌注损伤的影响，并初步探讨其作用机制。方法 夹闭大鼠左侧肾动、静脉45 min再放开恢复血流24 h，造成急性缺血再灌注肾损伤模型（2周前已切除右肾）；再灌注前3 d及缺血前1 h以埃他卡林溶液灌胃；应用放免法检测血清内皮素（ET）-1水平,应用RT-PCR法观察ET-1 mRNA在肾组织的表达。结果 缺血再灌注导致明显的肾脏功能与结构损伤。组织病理示肾小管上皮细胞变性，核浓缩、破碎，刷状缘坏死脱落，管腔内有细胞及蛋白管型,间质充血、水肿；血清ET-1水平升高，肾组织ET-1 mRNA表达升高。与缺血再灌注组比较，埃他卡林能明显降低血清尿素氮和肌酐水平，改善上述病理变化，降低血清ET-1水平，降低肾组织ET-1 mRNA的高表达。结论 埃他卡林对大鼠肾脏缺血再灌注损伤具有明显的保护作用。 |
| 关 键 词： | 埃他卡林 再灌注损伤 内皮缩血管肽类 |
| 收稿时间： | 2007-11-15 |
Protection of iptakalim hydrochloride on renal ischemia-reperfusion injury in rats |
|
| L Chang-ying,LONG Chao-liang,GAO Jun-yu,WANG Hai.Protection of iptakalim hydrochloride on renal ischemia-reperfusion injury in rats[J].Foreign Medical Sciences(Section of Pharmarcy),2008,35(2):81-86. | |
| Authors: | L Chang-ying LONG Chao-liang GAO Jun-yu WANG Hai |
| Affiliation: | L(U) Chang-ying,LONG Chao-liang,GAO Jun-yu,WANG Hai |
| Abstract: | Objective To study the protection and mechanism of iptakalim hydrochloride ( Ipt ) on ischemia-reperfusion(I/R) injury in the kidneys of rats. Methods On the 15th day following right nephrectomy, under anesthesia, a latero-abdominal laparotomy was made, and using minimal dissection, the left renal pedicle was isolated. The left renal pedicle was occluded for 45 min to induce ischemia and then subjected for 24 h of reperfusion to make I/R induced renal injury model. Ipt was orally gavaged at the doses of 0.5,1.0,1.5 and 2.0 mg·kg-1·d-1 for 3 d and 1 h before ischemia. The concentration of serum endothelin-1 (ET-1 ) was evaluated by radioimmunoassay, the expression of ET-1 mRNA in the kidneys of rats was detected by RT-PCR. Results I/R injury caused a significant deterioration in the renal function, pretreatment with Ipt significantly improved the functional and histological parameters, decreased the concentration of ET-1 and inhibited the expression of ET-1 mRNA in kidneys induced by renal I/R injury. Conclusion Ipt has a protective effect on renal I/R injury in rats. |
| Keywords: | iptakalim hydrochloride reperfusion injury endothelins |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《国际药学研究杂志》浏览原始摘要信息 | |
| 点击此处可从《国际药学研究杂志》下载全文 | |
