| 胆固醇酯转移蛋白转基因家兔制作及生物学特性分析 |
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| 引用本文： | 高守翠,成大欣,赵四海,陈玉龙,王晓靖,白亮,范江霖,刘恩岐.胆固醇酯转移蛋白转基因家兔制作及生物学特性分析[J].实验动物与比较医学,2015,23(4):331-335. |
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| 作者姓名： | 高守翠 成大欣 赵四海 陈玉龙 王晓靖 白亮 范江霖 刘恩岐 |
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| 作者单位： | 西安交通大学,西安交通大学,西安交通大学,西安交通大学,西安交通大学,西安交通大学,西安交通大学,西安交通大学 |
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| 摘 要： | 摘要: 目的 制作肝脏特异性高表达人胆固醇酯转移蛋白（CETP）转基因家兔并对其生物学特性分析进行分析。方法 利用显微注射方法制作CETP转基因家兔，利用Western Blot、Real-time PCR和CETP活性鉴定试剂盒鉴定模型家兔中人CETP转基因的表达和活性。结果 PCR检验结果显示我们成功获得CETP转基因家兔，转基因主要在肝脏特异性表达，其血浆CETP活性相对于非转基因家兔明显升高。结论 本研究首次成功建立了高表达人CETP的转基因家兔，为研究CETP功能和其参与心血管疾病的机制提供了良好模型。
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| 关 键 词： | 胆固醇酯转移蛋白 转基因 家兔 |
| 收稿时间： | 2015/1/22 0:00:00 |
| 修稿时间： | 2015/1/31 0:00:00 |
Establishment of cholesteryl ester transfer protein transgenic rabbits by DNA microinjection and analysis of their biological properties |
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| GAO Shou-cui,CHENG Da-xin,ZHAO Si-hai,CHEN Yu-long,WANG Xiao-jing,BAI Liang,FAN Jiang-lin and LIU En-qi.Establishment of cholesteryl ester transfer protein transgenic rabbits by DNA microinjection and analysis of their biological properties[J].Laboratory Animal and Comparative Medicine,2015,23(4):331-335. |
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| Authors: | GAO Shou-cui CHENG Da-xin ZHAO Si-hai CHEN Yu-long WANG Xiao-jing BAI Liang FAN Jiang-lin and LIU En-qi |
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| Affiliation: | Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory Animal Center, Xi''an Jiaotong University medical School, Xi''an 710061, China;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Department of Molecular Pathology, Faculty of Medicine, Yamanashi University, Yamanashi 409-3898, Japan;Laboratory for Lipid Metabolism and Atherosclerosis, Xi''an Jiaotong University Cardiovascular Research Center;Laboratory Animal Center, Xi''an Jiaotong University medical School, Xi''an 710061, China |
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| Abstract: | Objective The aim of this study was to generate human cholesteryl ester transfer protein (CETP) transgenic rabbits and analyze their biological properties. Methods We generated human CETP transgenic rabbits by DNA microinjection, and detected the expression of human CETP by real-time PCR and Western blot assay. The activity of CETP was measured using an activity assay kit. Results Human CETP transgenic rabbits were successfully generated by DNA microinjection. Compared with wide type rabbits, the expression of human CETP was dramatically increased in the liver of the human CETP transgenic rabbits. The plasma CETP activity was also much higher in the liver of human CETP transgenic rabbits than that of control rabbits. Conclusions The model of human CETP transgenic rabbits is successfully established by DNA microinjection. It will provide a useful tool for the studies of CETP biological function and its involvement in the mechanisms of cardiovascular diseases. |
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| Keywords: | cholesteryl ester transfer protein transgene rabbits |
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