| circ_0001178靶向miR-1179调控胃癌AGS细胞增殖和凋亡的机制探讨 |
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| 引用本文： | 王计,吴小微,晏妮.circ_0001178靶向miR-1179调控胃癌AGS细胞增殖和凋亡的机制探讨[J].蚌埠医学院学报,2022,47(11):1492-1496. |
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| 作者姓名： | 王计 吴小微 晏妮 |
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| 作者单位： | 武汉科技大学附属汉阳医院 消化内科, 湖北 武汉 430050 |
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| 基金项目： | 国家卫生计生委医药卫生科技发展研究中心课题W2015JZC30 |
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| 摘 要： | 目的探讨circ_0001178靶向miR-1179调控胃癌AGS细胞增殖和凋亡的机制。方法选取33例胃癌组织及癌旁组织标本，实时荧光定量PCR检测circ_0001178和miR-1179的表达水平；将胃癌细胞株AGS随机分为si-NC组、si-circ_0001178组、miR-NC组、miR-1179组、si-circ_0001178+anti-miR-NC组、si-circ_0001178+anti-miR-1179组；采用克隆形成实验检测克隆形成数，MTT法检测细胞活性，流式细胞术检测细胞凋亡，Western blotting法检测蛋白表达；采用双荧光素酶报告实验检测circ_0001178和miR-1179的靶向关系。结果胃癌组织中circ_0001178表达水平明显高于癌旁组织，而miR-1179表达水平明显低于癌旁组织（P < 0.01）。抑制circ_0001178表达或过表达miR-1179，AGS细胞克隆形成数减少，细胞活性降低，AGS细胞凋亡率升高，cleaved-caspase3和cleaved-caspase9表达水平均升高（P < 0.05）。结论抑制circ_0001178表达可能通过靶向上调miR-1179抑制胃癌AGS细胞增殖，诱导细胞凋亡。
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| 关 键 词： | 胃肿瘤 增殖 凋亡 circ_0001178 miR-1179 |
| 收稿时间： | 2021-04-10 |
Study on the mechanism of circ_0001178 targeting miR-1179 to regulate the proliferation and apoptosis of gastric cancer AGS cells |
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| Affiliation: | Department of Gastroenterology, Hanyang Hospital Affiliated to Wuhan University of Science and Technology, Wuhan Hubei 430050, China |
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| Abstract: | ObjectiveTo explore the mechanism of circ_0001178 targeting miR-1179 to regulate the proliferation and apoptosis of gastric cancer AGS cells.MethodsThe expression levels of circ_0001178 and miR-1179 in 33 samples of gastric cancer tissue and adjacent tissues were detected using the real-time fluorescent quantitative PCR.The gastric cancer cell line AGS was randomly divided into the si-NC group, si-circ_0001178 group, miR-NC group, miR-1179 group, si-circ_0001178+anti-miR-NC group and si-circ_0001178+anti-miR-1179 group.The number of clone formation was detected using the clone formation experiment, and the cell viability was detected using MTT assay.The apoptosis and protein expression were detected using the flow cytometry and Western blotting, respectively.The dual luciferase reporter assay was used to detect the targeting relationship between circ_0001178 and miR-1179.ResultsThe expression level of circ_0001178 in cancer tissue was higher than that in adjacent tissues, while the expression level of miR-1179 in cancer tissue was lower than that in adjacent tissues(P < 0.01).The inhibition of circ_0001178 expression or overexpression of miR-1179 could reduce the number of AGS cell clone formation, decrease the cell activity, increase the AGS cell apoptosis rate, and increase the expression levels of cleaved-caspase3 and cleaved-caspase9(P < 0.05).ConclusionsInhibiting the expression of circ_0001178 may inhibit the proliferation of gastric cancer AGS cells, and induce cell apoptosis by targeting up-regulation of miR-1179. |
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