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广西莪术挥发油抗肿瘤作用的谱效关系研究
引用本文:曾建红,莫炫永,戴平,黄凤香,廖迎,王建红,陈旭.广西莪术挥发油抗肿瘤作用的谱效关系研究[J].中国实验方剂学杂志,2012,18(13):91-94.
作者姓名:曾建红  莫炫永  戴平  黄凤香  廖迎  王建红  陈旭
作者单位:1. 桂林医学院药学院,广西桂林,541004
2. 东莞市国药质量检测中心,广州东莞,523010
基金项目:国家自然科学基金项目(30960498);广西教育厅科研项目(200810MS143)
摘    要:目的:探讨广西莪术挥发油气相色谱-质谱联用仪(GC-MS)指纹图谱所代表的“有效组分群”对药效贡献的大小,为中药的质量控制和药效评价标准的制定提供参考.方法:通过GC-MS分析技术建立广西莪术挥发油指纹图谱,并对广西莪术挥发油的抗肿瘤作用进行研究,在获得的挥发油GC-MS指纹图谱特征峰面积与对鼻咽癌细胞的增殖抑制作用的数据基础上,采用灰关联度分析技术,计算各特征峰与对肿瘤细胞的增殖抑制率的关联度和关联序.结果:依据关联度的大小,确定各成分对抗肿瘤作用贡献的大小顺序为莪术醇>莪术二酮>β-榄香烯>吉马酮>莪术酮> α-丁子香烯>δ-瑟林烯>β-石竹烯>β-榄香烯酮>a-蒎烯>α-石竹烯>桉油素>樟脑>2-壬醇>异龙脑莪术烯>δ-榄香烯>莰烯>D-柠檬烯>龙脑.结论:广西莪术抗肿瘤作用是其内“有效组分群”共同作用的结果,本研究确定了广西莪术挥发油“有效组分群”指纹特征对药效贡献的大小,为中药的“谱效关联”的指纹图谱的建立、中药的质量控制和药效评价标准的制定提供了新的思路和方法.

关 键 词:广西莪术  挥发油  指纹图谱  抗肿瘤作用  灰关联度  谱效关系
收稿时间:2012/2/21 0:00:00

Study on Spectrum-effect Relationship betweent Fingerprint of Essential Oil and of Anti-tumor Effect from Curcuma kwangsiensis
ZENG Jian-hong,MO Xuan-yong,DAI Ping,HUANG Feng-xiang,LIAO Ying,WANG Jian-hong and CHEN Xu.Study on Spectrum-effect Relationship betweent Fingerprint of Essential Oil and of Anti-tumor Effect from Curcuma kwangsiensis[J].China Journal of Experimental Traditional Medical Formulae,2012,18(13):91-94.
Authors:ZENG Jian-hong  MO Xuan-yong  DAI Ping  HUANG Feng-xiang  LIAO Ying  WANG Jian-hong and CHEN Xu
Affiliation:School of Pharmacy, Guilin Medical College, Guilin 541004, China;Guoyao Quality Inspection Center in Dongguan City, Dongguan 523010, China;School of Pharmacy, Guilin Medical College, Guilin 541004, China;School of Pharmacy, Guilin Medical College, Guilin 541004, China;School of Pharmacy, Guilin Medical College, Guilin 541004, China;School of Pharmacy, Guilin Medical College, Guilin 541004, China;School of Pharmacy, Guilin Medical College, Guilin 541004, China
Abstract:Objective:To investigate the effective component group represented by fingerprint contributed to pharmacodynamic by grey relational analysis,and provide a reference for traditional Chinese medicine quality control and develop evaluation criteria of pharmacodynamics.Method: The fingerprint of essential oil from Curcuma kwangsiensis was established by gas chromatography-mass spectrometry(GC-MS),and the anti-tumor effect of curcuma oil was studied.At the premise of the acquired GC-MS characteristic of fingerprint and pharmacodynamic data to inhibiting proliferation of nasopharyngeal carcinoma cell,the relational grade and relational sequence between fingerprint of essential oil and of inhibiting proliferation rate were calculated by grey relational analysis.Result: According to the relational grade the sequence of contribution to inhibiting proliferation to nasopharyngeal carcinoma cell was curcumol>curdione>β-elemene>germacrone>curzerenone>α-caryophyllene>δ-selinene>β-caryophyllene>β-elemenone>a-pinene>α-caryophyllene>eucalyptol>camphor>2-nonanol>isoborneol>curzerene>δ-elemene>camphene>D-limonene>borneol.Conclusion: The anti-tumor effect of C.kwangsiensis is the result of combined effect of ’effective component group.This study identifies the effective component group represented by fingerprint contributed to pharmacodynamic,and provides a reference for establishing fingerprint of traditional Chinese medicine’s spectrum-effect relationship,traditional Chinese medicine quality control and develop evaluation criteria of pharmacodynamics.
Keywords:Curcuma kwangsiensis  essential oil  fingerprint  anti-tumor effect  grey relational analysis  spectrum-effect relationship
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