| 三七总皂苷对原代培养大鼠皮层神经细胞的保护作用 |
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| 引用本文： | 马丽焱,肖培根,梁发权,武军华.三七总皂苷对原代培养大鼠皮层神经细胞的保护作用[J].中国药学杂志,1998(3). |
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| 作者姓名： | 马丽焱 肖培根 梁发权 武军华 |
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| 作者单位： | 1. 北京 100094 中国医学科学院-中国协和医科大学药用植物研究所
2. 北京 100850 军事医学科学院毒物药物研究所 |
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| 摘 要： | 目的：研究三七总皂苷（ＰＮＳ）对神经细胞的保护作用。方法：采用原代培养的大鼠大脑皮层神经细胞，观察谷氨酸（５００μｍｏｌ·Ｌ－１，８ｍｉｎ），缺氧５ｈ再给氧不同时间（０，３，２４ｈ）对细胞的影响以及ＰＮＳ的作用。结果：ＰＮＳ２５，５０ｍｇ·Ｌ－１明显减少缺氧／再给氧损伤时细胞内酶的释放，减轻细胞形态学的改变，提高细胞的存活率。对谷氨酸介导的兴奋性毒性，ＰＮＳ也有一定的拮抗作用。结论：ＰＮＳ的脑保护作用机制可能与其抗兴奋性毒性和缺氧性损伤的作用有关。
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| 关 键 词： | 三七总皂苷 神经细胞培养 兴奋性毒性 缺氧／再给氧 |
Protective effects of Panax notoginseng saponins on primary cortical cultures of rat |
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| Abstract: | OBJECTIVE: To study the neuroprotective effects of saponins of Panax notoginseng (PNS). METHODS: Using primary cultured rat cortical neurons, we observed the influences of PNS on hypoxia (5 h)/ reoxygenation (0, 3, 24 h) and on excitotoxicity of glutamate (500 μmolL-1, 8 min). RESULTS: PNS 25, 50 mgL-1 attenuated neuronal damage during hypoxia/reoxygenation, which was reflected by decrease of enzyme release from cells into culture medium and reduction of changes in cell morphology. PNS, furthermore, prevented neurons from the excitotoxic effect of glutamate. CONCLUSION: The anticerebral ischemia effect of PNS partially contributed to its neuroprotective activities. |
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| Keywords: | saponins of Panax notoginseng neuron culture excitotoxicity hypoxia/reoxygeneration |
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