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| 四物汤对放射线致血虚证小鼠骨髓蛋白质表达的影响 | |
| 引用本文： | 郭平,马增春,李鹰飞,梁乾德,王继峰,王升启.四物汤对放射线致血虚证小鼠骨髓蛋白质表达的影响[J].中国中药杂志,2004,29(9):893-896. |
| 作者姓名： | 郭平 马增春 李鹰飞 梁乾德 王继峰 王升启 |
| 作者单位： | 1. 军事医学科学院,放射医学研究所,北京,100850;北京中医药大学,北京100029 2. 军事医学科学院,放射医学研究所,北京,100850 3. 北京中医药大学,北京100029 |
| 基金项目： | 国家自然科学基金，军队科技攻关项目，首都创新工程项目(248项目) |
| 摘 要： | 目的 :观察四物汤对血虚证小鼠骨髓蛋白质表达的影响 ,为阐明四物汤补血作用的分子机理提供理论和实验依据。方法 :采用 3.5Gy60Coγ射线全身 1次性照射 ,制备小鼠血虚证模型。利用双向电泳、图像分析、胶内酶切、质谱鉴定等蛋白质组学技术 ,结合生物信息学 ,分离、分析、鉴定蛋白质。结果 :四物汤可以逆转放射线致血虚证小鼠骨髓 10个上调和 5个下调的蛋白质。其中 8个蛋白质可能分别是淋巴细胞特异性蛋白质 1、蛋白酶体26SATP酶亚组分 4、造血细胞蛋白质酪氨酸磷酸酶、H-ras、3-磷酸甘油醛脱氢酶、生长因子受体结合蛋白 14及Lgals12。结论 :四物汤能调节骨髓蛋白质表达 ,并可能由此促进造血细胞的生长和分化 ,发挥补血作用。 |
| 关 键 词： | 四物汤 辐射 血虚证 功能蛋白质组学 骨髓 |
| 文章编号： | 1001-5302(2004)09-0893-04 |
| 收稿时间： | 2004/4/15 0:00:00 |
Effects of Siwu Tang on protein expression of bone marrow of blood deficiency mice induced by irradiation |
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| GUO Ping ;MA Zeng-chun ;LI Ying-fei ;LIANG Qian-de ;W ANG Ji-feng ;WANG Sheng-qi.Effects of Siwu Tang on protein expression of bone marrow of blood deficiency mice induced by irradiation[J].China Journal of Chinese Materia Medica,2004,29(9):893-896. | |
| Authors: | GUO Ping ;MA Zeng-chun ;LI Ying-fei ;LIANG Qian-de ;W ANG Ji-feng ;WANG Sheng-qi |
| Affiliation: | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. |
| Abstract: | OBJECTIVE: To observe the effects of siwu tang on protein expression of bone marrow of blood deficiency mice and provide the theoretical and experimental basis for understanding the molecular mechanism of blood enriching function of siwu tang. METHOD: Blood deficiency mice were established by using 3.5 Gy 60Co gamma-ray. With proteomic technologies including two-dimensional electrophoresis, image analysis, in-gel digestion, peptide mass fingerprinting and bioinformatics the proteins of bone marrow of blood deficiency mice were isolated, analyzed, and identified. RESULT: Siwu tang could reverse 10 up-regulated and 4 down-regulated proteins of blood deficiency mice bone marrow. Seven of the proteins were likely to be lymphocyte specific protein 1, proteasome 26S ATPase subunit 4, hematopoietic cell protein-tyrosine phosphatase, glyceraldehyde-3-phosphate dehydrogenase, growth factor receptor binding protein 14 and lgals12 respectively. CONCLUSION: Siwu tang can regulate the protein expression of bone marrow of blood deficiency mice and thus promote the growth and differentiation of hematopoietic cells and then exert its effects on blood enriching function. |
| Keywords: | Siwu Tang irradiation blood deficiency functional proteomics bone morrow |
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