| 苦参碱和氧化苦参碱对SMMC-7721细胞增殖及Stat3,Stat5基因表达的影响 |
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| 引用本文： | 郑艳敏,李轩,赵红艳,赵军艳.苦参碱和氧化苦参碱对SMMC-7721细胞增殖及Stat3,Stat5基因表达的影响[J].中国中药杂志,2008,33(19):2234-2237. |
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| 作者姓名： | 郑艳敏 李轩 赵红艳 赵军艳 |
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| 作者单位： | 武警医学院,附属医院,消化内科,天津,300162 |
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| 摘 要： | 目的：观察苦参碱和氧化苦参碱对肝癌细胞SMMC-7721增殖及Stat3, Stat5基因表达的影响。方法：苦参碱和氧化苦参碱干预肝癌细胞SMMC-7721，MTT法检测其对肝癌细胞增殖的影响，双荧光染色观察细胞凋亡率，RT-PCR法检测其对SMMC-7721细胞Stat3, Stat5 mRNA的影响。结果：苦参碱和氧化苦参碱作用于肝癌细胞后，能显著抑制肝癌细胞增殖，促进其凋亡，并呈时间剂量依赖性；二者均能下调Stat3, Stat5 mRNA表达水平。以相同浓度苦参碱和氧化苦参碱比较，苦参碱对肝癌细胞Stat3, Stat5 mRNA下调作用更显著(P<0.05或P<0.01)。结论：苦参碱和氧化苦参碱能显著抑制SMMC-7721细胞增殖，其机制可能与下调Stat3, Stat5的基因表达，抑制细胞信号传导通路有关。
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| 关 键 词： | 苦参碱 氧化苦参碱 信号传导 Stat3 Stat5 |
| 收稿时间： | 2007/11/10 0:00:00 |
Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line |
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| ZHENG Yan-min; LI Xuan; ZHAO Hong-yan; ZHAO Jun-yan.Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line[J].China Journal of Chinese Materia Medica,2008,33(19):2234-2237. |
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| Authors: | ZHENG Yan-min; LI Xuan; ZHAO Hong-yan; ZHAO Jun-yan |
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| Abstract: | Objective: To study the effect of matrine and oxymatrine on proliferation and the expression of Stat3, Stat5 mRNA in SMMC-7721 cell line. Method: Treated with matrine and oxymatrine, the inhibitory effect on SMMC-7721 cell proliferation was detected by MTT, double fluorescence labeling was applied to measure the apotosis ratios of SMMC-7721cells, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell line were assessed with RT-PCR. Result: Matrine and oxymatrine could inhibit the proliferation and induce the apoptosis of SMMC-7721 cells and it was time and dose dependent, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell with matrine and oxymatrine were significantly lower than those in control group (P<0.05 or P<0.01). Compared with the same dose of matrine and oxymatrine, matrine showed stronger effect on cell proliferation, apoptosis, and Stat3 and Stat5 mRNA (P<0.05 or P<0.01). Conclusion: Matrine and oxymatrine inhibited the proliferation and induced the of SMMC-7721 cells significantly, the mechanism of which might be related to the down-regulation of stat3 and stat5 mRNA and inhibition of the signaling transduction pathway. |
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| Keywords: | matrine oxymatrine signaling pathway Stat3 Stat5 |
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