| Ti和Ri质粒对栝楼双转化的研究 |
| |
| 引用本文： | 雷和田,宋经元,祁荫麟,张荫麟,杨峻山,郭志刚.Ti和Ri质粒对栝楼双转化的研究[J].中国中药杂志,2001,26(3):162-165. |
| |
| 作者姓名： | 雷和田 宋经元 祁荫麟 张荫麟 杨峻山 郭志刚 |
| |
| 作者单位： | 1. 中国医学科学院 协和医科大学 药用植物研究所，北京 100094
2. 清华大学 化学工程系，北京 100084 |
| |
| 摘 要： | 目的 :建立栝楼双转化毛状根培养系统。方法 :①利用根癌农杆菌Agrobacterium tumefaciens菌株C58感染栝楼Trichosanthes kirilowii.无菌苗诱导冠瘿瘤 ,然后用发根农杆菌Agrobacterium rhizogenes菌株 15834感染其再生植株 ,诱导出毛状根 ;②用纸电泳检测Ti和Ri质粒是否转化成功 ;③运用分光光度计和SDS PAGE检测栝楼组织中所含蛋白。结果 :Ti和Ri质粒转化成功并建立了栝楼双转化毛状根培养系统。结论 :双转化毛状根与一般毛状根相比 ,具有生长更迅速等特点而蛋白含量基本一样 ;因此 ,利用它来生产天花粉蛋白更具有开发潜力。
|
| 关 键 词： | 栝楼 冠瘿瘤 双转化毛状根 |
| 文章编号： | 1001-5302(2001)03-0162-04 |
| 收稿时间： | 2000/5/11 0:00:00 |
| 修稿时间： | 2000年5月11日 |
Genetic Transformation of Hairy Roots in Trichosanthes kirilowii Maxim.by Ti and Ri Plasmids |
| |
| LEI He-tian;SONG Jang-juan;QI Jian-jun;ZHANG Yin-lin;YANG Jun-shan;GUO Zhi-gang.Genetic Transformation of Hairy Roots in Trichosanthes kirilowii Maxim.by Ti and Ri Plasmids[J].China Journal of Chinese Materia Medica,2001,26(3):162-165. |
| |
| Authors: | LEI He-tian;SONG Jang-juan;QI Jian-jun;ZHANG Yin-lin;YANG Jun-shan;GUO Zhi-gang |
| |
| Affiliation: | Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China. |
| |
| Abstract: | OBJECTIVE: To establish a hairy root culture system by double transformation for Trichosanthes kirilowii. METHOD: 1. Crown galls were induced by direct infection of sterile seedlings of T. kirilonii with Agrobacterium tumefaciens C58, and then the hairy roots were obtained from the regenerated plants by infection with A. rhzogenes 15834; 2. Transformation of Ti and Ri plasmids was inspected by high-pressure-paper electrophoresis; 3. The protein contents in the tissues of T. kirilowii were inspected by spectrophotometer and SDS-PAGE. RESULT: A hairy root culture system has been established successfully by double transformation with Ti and Ri plasmids in T. kirilowii. CONCLUSION: Compared with the ordinary hairy roots, the double transformed hairy roots grow faster but retain similar protein contents. |
| |
| Keywords: | T kirilowii hairy roots double transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
