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HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量
引用本文:古丽娜·沙比尔,阿吉艾克拜尔·艾萨,石明辉,贾晓光.HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量[J].中国中药杂志,2007,32(24):2619-2622.
作者姓名:古丽娜·沙比尔  阿吉艾克拜尔·艾萨  石明辉  贾晓光
作者单位:1. 新疆中药民族药研究所,新疆,乌鲁木齐,830002
2. 中国科学院,新疆理化技术研究所,新疆,乌鲁木齐,830011
基金项目:新疆维吾尔自治区少数民族科技人才特殊培养专项基金(2005-6)
摘    要:目的:建立同时测定苦豆子中槐定碱、氧化槐果碱及氧化苦参碱含量的HPLC测定法。方法:色谱柱为Phenomenex Gemini C18(46 mm×250 mm,5 μm),以甲醇(A)-02%磷酸水(B)为流动相,检测波长205 nm,流速1 mL·min-1,柱温30 ℃。结果:在线性范围内3个对照品的标准曲线呈良好的线性关系(r≥0999 7)。平均回收率分别为1000%,970%,970%。结论:可通过HPLC同时测定苦豆子中槐定碱、氧化槐果碱和氧化苦参碱的含量,并可用该方法进行苦豆子药材的质量控制。

关 键 词:苦豆子  HPLC法  槐定碱  氧化槐果碱  氧化苦参碱
文章编号:1001-5302(2007)24-2619-04
收稿时间:3/1/2007 12:00:00 AM
修稿时间:2007年3月1日

Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC
Gulnar Sabir; Haji Akber Aisa; Shi Ming-hui; JIA Xiao-guang.Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC[J].China Journal of Chinese Materia Medica,2007,32(24):2619-2622.
Authors:Gulnar Sabir; Haji Akber Aisa; Shi Ming-hui; JIA Xiao-guang
Affiliation:Xingjiang Institute of Chinese Material Medica and Ethica, Urumch 830002, China. gulnar_sabir@163.com
Abstract:OBJECTIVE: To develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides. METHOD: Chromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C. RESULT: All calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively. CONCLUSION: This method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed.
Keywords:Sophora alopecuroides seed  HPLC  sophoridine  oxysophocarpine  oxymatrine
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